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GNAT2 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GNAT2
Gene Name:  guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 2
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH110170.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00002969

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1065nts         Open reading frame : 1 to 1065
Reference Sequence Alignment

ATGGGAAGTGGAGCCAGTGCTGAGGACAAAGAACTGGCCAAGAGGTCCAAGGAGCTAGAA
AAGAAGCTGCAGGAGGATGCTGATAAGGAAGCCAAGACTGTCAAGCTGCTACTGCTGGGT
GCTGGGGAGTCAGGAAAGAGCACCATCGTCAAACAGATGAAGATCATTCACCAGGATGGC
TATTCACCAGAAGAATGCCTGGAGTTCAAGGCTATCATCTATGGAAATGTGCTGCAGTCC
ATCCTGGCTATCATCCGGGCCATGACCACACTGGGCATCGATTATGCTGAACCAAGCTGT
GCGGATGACGGGCGACAGCTCAACAACCTGGCTGACTCCATTGAGGAGGGAACCATGCCT
CCTGAGCTCGTGGAGGTCATTAGGAGGTTGTGGAAGGATGGTGGGGTGCAAGCCTGCTTC
GAGAGAGCTGCAGAATACCAGCTTAATGACTCCGCATCTTACTACCTGAACCAATTAGAA
CGAATTACAGACCCTGAGTACCTCCCTAGTGAGCAAGATGTGCTCCGATCCAGAGTCAAA
ACCACAGGCATCATTGAAACCAAGTTTTCCGTCAAAGACTTGAATTTCAGGATGTTTGAT
GTGGGAGGGCAGAGATCCGAGAGAAAGAAGTGGATCCACTGCTTCGAGGGAGTCACCTGC
ATCATTTTCTGTGCAGCCCTCAGTGCCTATGATATGGTGCTGGTGGAAGATGACGAAGTG
AATCGTATGCATGAGTCTTTGCATCTGTTCAACAGCATATGTAACCACAAATTCTTTGCG
GCTACTTCCATTGTCCTCTTTCTCAACAAGAAGGACCTCTTTGAGGAAAAAATCAAGAAA
GTCCATCTCAGCATTTGTTTTCCAGAGTATGATGGTAACAACTCCTATGATGATGCGGGG
AATTACATAAAGAGCCAGTTCCTTGACCTCAATATGCGAAAAGATGTCAAAGAAATCTAC
AGTCACATGACCTGTGCTACAGATACACAGAATGTCAAATTTGTGTTTGATGCAGTTACA
GATATTATCATCAAAGAAAACCTCAAGGACTGCGGCCTCTTCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1246
Start on reference sequence 182
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GNAT2
Symbol Nomenclature : GNAT2
SYNONYM : ACHM4; GNATC
Designation : cone-type transducin alpha subunit|guanine nucleotide-binding protein G(t) subunit alpha-2|transducin alpha-2 chain|transducin, cone-specific, alpha polypeptide
Full Nomenclature : guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 2
GENEID : 2780
Locus Tag : RP5-1160K1.4
GI : 60655274
GenBank Accession : AY889410
HGNC : 4394
MIM : 139340
Vega : OTTHUMG00000011639
Target GenBank: BC000233

 

Reference Sequence Alignment

  

HsCD00002969      ATGGGAAGTGGAGCCAGTGCTGAGGACAAAGAACTGGCCAAGAGGTCCAAGGAGCTAGAA
NM_005272.3       ATGGGAAGTGGAGCCAGTGCTGAGGACAAAGAACTGGCCAAGAGGTCCAAGGAGCTAGAA
                  ************************************************************

HsCD00002969      AAGAAGCTGCAGGAGGATGCTGATAAGGAAGCCAAGACTGTCAAGCTGCTACTGCTGGGT
NM_005272.3       AAGAAGCTGCAGGAGGATGCTGATAAGGAAGCCAAGACTGTCAAGCTGCTACTGCTGGGT
                  ************************************************************

HsCD00002969      GCTGGGGAGTCAGGAAAGAGCACCATCGTCAAACAGATGAAGATCATTCACCAGGATGGC
NM_005272.3       GCTGGGGAGTCAGGAAAGAGCACCATCGTCAAACAGATGAAGATCATTCACCAGGATGGC
                  ************************************************************

HsCD00002969      TATTCACCAGAAGAATGCCTGGAGTTCAAGGCTATCATCTATGGAAATGTGCTGCAGTCC
NM_005272.3       TATTCACCAGAAGAATGCCTGGAGTTCAAGGCTATCATCTATGGAAATGTGCTGCAGTCC
                  ************************************************************

HsCD00002969      ATCCTGGCTATCATCCGGGCCATGACCACACTGGGCATCGATTATGCTGAACCAAGCTGT
NM_005272.3       ATCCTGGCTATCATCCGGGCCATGACCACACTGGGCATCGATTATGCTGAACCAAGCTGT
                  ************************************************************

HsCD00002969      GCGGATGACGGGCGACAGCTCAACAACCTGGCTGACTCCATTGAGGAGGGAACCATGCCT
NM_005272.3       GCGGATGACGGGCGACAGCTCAACAACCTGGCTGACTCCATTGAGGAGGGAACCATGCCT
                  ************************************************************

HsCD00002969      CCTGAGCTCGTGGAGGTCATTAGGAGGTTGTGGAAGGATGGTGGGGTGCAAGCCTGCTTC
NM_005272.3       CCTGAGCTCGTGGAGGTCATTAGGAGGTTGTGGAAGGATGGTGGGGTGCAAGCCTGCTTC
                  ************************************************************

HsCD00002969      GAGAGAGCTGCAGAATACCAGCTTAATGACTCCGCATCTTACTACCTGAACCAATTAGAA
NM_005272.3       GAGAGAGCTGCAGAATACCAGCTTAATGACTCCGCATCTTACTACCTGAACCAATTAGAA
                  ************************************************************

HsCD00002969      CGAATTACAGACCCTGAGTACCTCCCTAGTGAGCAAGATGTGCTCCGATCCAGAGTCAAA
NM_005272.3       CGAATTACAGACCCTGAGTACCTCCCTAGTGAGCAAGATGTGCTCCGATCCAGAGTCAAA
                  ************************************************************

HsCD00002969      ACCACAGGCATCATTGAAACCAAGTTTTCCGTCAAAGACTTGAATTTCAGGATGTTTGAT
NM_005272.3       ACCACGGGCATCATTGAAACCAAGTTTTCCGTCAAAGACTTGAATTTCAGGATGTTTGAT
                  *****.******************************************************

HsCD00002969      GTGGGAGGGCAGAGATCCGAGAGAAAGAAGTGGATCCACTGCTTCGAGGGAGTCACCTGC
NM_005272.3       GTGGGAGGGCAGAGATCCGAGAGAAAGAAGTGGATCCACTGCTTCGAGGGAGTCACCTGC
                  ************************************************************

HsCD00002969      ATCATTTTCTGTGCAGCCCTCAGTGCCTATGATATGGTGCTGGTGGAAGATGACGAAGTG
NM_005272.3       ATCATTTTCTGTGCAGCCCTCAGTGCCTATGATATGGTGCTGGTGGAAGATGACGAAGTG
                  ************************************************************

HsCD00002969      AATCGTATGCATGAGTCTTTGCATCTGTTCAACAGCATATGTAACCACAAATTCTTTGCG
NM_005272.3       AATCGTATGCATGAGTCTTTGCATCTGTTCAACAGCATATGTAACCACAAATTCTTTGCG
                  ************************************************************

HsCD00002969      GCTACTTCCATTGTCCTCTTTCTCAACAAGAAGGACCTCTTTGAGGAAAAAATCAAGAAA
NM_005272.3       GCTACTTCCATTGTCCTCTTTCTCAACAAGAAGGACCTCTTTGAGGAAAAAATCAAGAAA
                  ************************************************************

HsCD00002969      GTCCATCTCAGCATTTGTTTTCCAGAGTATGATGGTAACAACTCCTATGATGATGCGGGG
NM_005272.3       GTCCATCTCAGCATTTGTTTTCCAGAGTATGATGGTAACAACTCCTATGATGATGCGGGG
                  ************************************************************

HsCD00002969      AATTACATAAAGAGCCAGTTCCTTGACCTCAATATGCGAAAAGATGTCAAAGAAATCTAC
NM_005272.3       AATTACATAAAGAGCCAGTTCCTTGACCTCAATATGCGAAAAGATGTCAAAGAAATCTAC
                  ************************************************************

HsCD00002969      AGTCACATGACCTGTGCTACAGATACACAGAATGTCAAATTTGTGTTTGATGCAGTTACA
NM_005272.3       AGTCACATGACCTGTGCTACAGATACACAGAATGTCAAATTTGTGTTTGATGCAGTTACA
                  ************************************************************

HsCD00002969      GATATTATCATCAAAGAAAACCTCAAGGACTGCGGCCTCTTCTAG
NM_005272.3       GATATTATCATCAAAGAAAACCTCAAGGACTGCGGCCTCTTCTAA
                  ********************************************.

Pathways

NCI : Visual signal transduction: Cones
Panther : Metabotropic glutamate receptor group II pathway
Panther : Muscarinic acetylcholine receptor 2 and 4 signaling pathway
Reactome : Ca2+ pathway
Reactome : G alpha (i) signalling events
Reactome : G-protein activation
Reactome : G-protein mediated events
Reactome : GPCR downstream signaling
Reactome : Opioid Signalling
Reactome : PLC beta mediated events
Reactome : Signal Transduction
Reactome : Signaling by GPCR
Reactome : Signaling by Wnt
Reactome : beta-catenin independent WNT signaling

Protein

SMART domain : G_alpha : G protein alpha subunit
UniProt : P19087
UniProt : Q5T697
HPRD : 00762

Clone

Cloning Information : 110170
HIP Master Clone ID : 86453
Original Clone ID : FLH110170.01X

Organism


TAX_ID : 9606
Species Specific ID: 2780

Genome

Chromosome : 1
Map Location : 1p13.1
Ensembl : ENSG00000134183

Reagents

Labome : GNAT2-antibody