Gene:
Gene Symbol:
ANO2
Gene Name:
anoctamin 2
Original Clone ID:
FLH269783.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297111
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCTGAAGATATACCCCTGCTCCCTGGCTCCCCACGCCGGCTGAGCCCTCAGGCAGGG
TCCAGAGGGGGCCAGGGCCCCAAACATGGACAGCAGTGTCTCAAGATGCCAGGTCCCCGG
GCCCCAGGTCTGCAGGGCGGTTCCAACAGAGATCCTGGCCAGCCCTGCGGTGGAGAGAGC
ACCCGCAGCAGCTCTGTCATCAACAACTATCTGGATGCCAATGAGCCTGTGTCCTTGGAG
GCCCGTCTTAGCCGCATGCACTTCCATGACAGTCAGAGGAAGGTCGACTATGTACTTGCC
TACCACTACCGGAAACGCGGGGTGCACCTGGCCCAAGGCTTCCCTGGCCACTCGCTGGCT
ATCGTCTCCAATGGGGAGACAGGCAAGGAGCCTCATGCTGGGGGCCCAGGTGACATTGAG
CTGGGACCGCTCGATGCCCTGGAGGAGGAGAGGAAGGAGCAGCGGGAGGAATTTGAGCAC
AATCTGATGGAGGCTGGACTGGAGCTTGAGAAGGACTTGGAGAATAAAAGCCAGGGATCC
ATCTTTGTCCGGATACACGCCCCGTGGCAGGTGCTGGCCAGAGAGGCAGAATTCTTGAAG
ATCAAAGTTCCTACCAAGAAAGAGATGTACGAGATCAAAGCAGGAGGCAGCATTGCAAAG
AAGTTCAGCGCGGCTCTGCAGAAGCTGAGCTCGCACCTGCAGCCCCGAGTTCCAGAACAC
AGCAACAACAAGATGAAAAACCTCTCCTACCCATTCTCCAGGGAGAAGATGTACCTGTAC
AACATCCAGGAAAAGGACACCTTCTTTGATAATGCCACCCGTAGCCGCATTGTGCACGAG
ATCCTGAAGCGCACAGCCTGCTCCCGAGCCAACAACACGATGGGTATTAACTCTCTGATC
GCAAACAATATCTATGAGGCTGCCTACCCTCTTCATGACGGTGAATACGATAGTCCAGAG
GACGATATGAATGACAGGAAGCTGCTATATCAAGAATGGGCGCGCTATGGAGTGTTCTAT
AAGTTCCAACCTATTGACCTCATCAGAAAGTATTTTGGAGAAAAAATTGGACTGTATTTT
GCCTGGCTGGGATTATATACATCATTCCTCATCCCATCTTCTGTAATTGGAGTGATTGTG
TTTCTTTATGGATGTGCAACAATTGAAGAAGATATTCCCAGCAGAGAGATGTGTGACCAG
CAGAATGCCTTCACCATGTGTCCCCTGTGTGACAAGTCCTGTGATTACTGGAACCTCAGC
TCAGCCTGTGGGACCGCGCAGGCCAGCCACCTGTTTGACAACCCTGCCACCGTCTTCTTC
TCTATCTTCATGGCTCTGTGGGCTACCATGTTCCTGGAAAACTGGAAGAGGCTACAGATG
CGACTGGGCTACTTTTGGGACCTGACTGGCATAGAAGAGGAAGAAGAACGTGCCCAGGAA
CATTCCAGGCCTGAGTATGAAACCAAAGTTCGAGAGAAAATGCTAAAGGAGAGCAACCAG
TCTGCTGTCCAGAAATTGGAAACAAACACGACGGAGTGTGGCGATGAGGATGATGAAGAT
AAACTGACCTGGAAGGATCGTTTCCCAGGTTACCTGATGAACTTTGCCTCCATCTTATTC
ATGATTGCCCTGACATTCTCAATCGTCTTTGGGGTGATAGTGTATCGAATAACAACTGCA
GCCGCTCTGTCTCTCAATAAGGCTACACGCTCCAATGTCCGGGTGACAGTGACAGCAACA
GCAGTCATCATCAACCTCGTGGTCATCCTCATCCTGGACGAGATCTACGGCGCTGTGGCC
AAGTGGCTCACCAAAATTGAGGTTCCGAAAACAGAACAGACTTTTGAAGAGCGCCTGATC
CTCAAAGCTTTCTTGCTCAAGTTTGTCAATGCCTACTCCCCCATCTTCTATGTGGCCTTT
TTCAAAGGGAGGTTTGTGGGCAGGCCTGGAAGCTACGTCTATGTATTCGATGGTTACCGC
ATGGAAGAGTGTGCTCCAGGGGGCTGTCTCATGGAGCTCTGCATTCAGCTCAGCATCATC
ATGTTGGGGAAGCAGTTGATCCAGAACAACATCTTTGAGATTGGAGTCCCGAAGCTAAAG
AAACTATTTCGAAAGCTGAAAGATGAGACCGAAGCTGGAGAAACTGACTCTGCCCATTCG
AAACATCCAGAGCAGTGGGACCTAGACTACAGCTTGGAACCATACACAGGACTGACTCCG
GAGTACATGGAAATGATCATCCAGTTTGGTTTTGTCACCCTCTTCGTGGCCTCCTTTCCC
CTGGCACCTGTGTTTGCCCTCCTCAACAACGTCATTGAAGTGCGGCTCGATGCAAAGAAG
TTTGTTACAGAGCTGAGACGGCCGGATGCTGTAAGAACCAAAGATATCGGAATCTGGTTT
GACATTCTCTCTGGAATTGGCAAGTTCTCTGTTATCAGCAACGCTTTTGTCATTGCGATC
ACCTCCGACTTTATCCCCCGCCTGGTGTACCAGTACTCCTACAGTCACAATGGGACTCTG
CACGGCTTTGTCAACCACACCCTCTCCTTTTTCAACGTCAGCCAGCTGAAGGAGGGGACG
CAGCCAGAAAACTCACAGTTTGACCAGGAGGTTCAGTTCTGCAGGTTTAAGGATTACCGA
GAGCCGCCATGGGCCCCGAACCCTTATGAGTTTTCGAAACAGTACTGGTTTATTCTGTCT
GCCCGTCTGGCTTTTGTCATAATCTTCCAGAACCTCGTGATGTTCCTGAGCGTCCTCGTG
GACTGGATGATTCCAGACATCCCCACGGACATCAGCGACCAGATCAAGAAAGAGAAGAGC
TTATTAGTGGATTTCTTCCTGAAAGAGGAGCATGAGAAGCTCAAGCTGATGGATGAGCCG
GCTCTGAGGAGCCCAGGAGGTGGGGATCGAAGCAGGAGCCGGGCAGCCAGCTCAGCACCT
TCAGGCCAAAGCCAGCTGGGCAGCATGATGTCGTCAGGCTCTCAGCACACCAATGTG
Protein sequence predicted based on nucleotide sequence
MPEDIPLLPGSPRRLSPQAGSRGGQGPKHGQQCLKMPGPRAPGLQGGSNRDPGQPCGGES TRSSSVINNYLDANEPVSLEARLSRMHFHDSQRKVDYVLAYHYRKRGVHLAQGFPGHSLA IVSNGETGKEPHAGGPGDIELGPLDALEEERKEQREEFEHNLMEAGLELEKDLENKSQGS IFVRIHAPWQVLAREAEFLKIKVPTKKEMYEIKAGGSIAKKFSAALQKLSSHLQPRVPEH SNNKMKNLSYPFSREKMYLYNIQEKDTFFDNATRSRIVHEILKRTACSRANNTMGINSLI ANNIYEAAYPLHDGEYDSPEDDMNDRKLLYQEWARYGVFYKFQPIDLIRKYFGEKIGLYF AWLGLYTSFLIPSSVIGVIVFLYGCATIEEDIPSREMCDQQNAFTMCPLCDKSCDYWNLS SACGTAQASHLFDNPATVFFSIFMALWATMFLENWKRLQMRLGYFWDLTGIEEEEERAQE HSRPEYETKVREKMLKESNQSAVQKLETNTTECGDEDDEDKLTWKDRFPGYLMNFASILF MIALTFSIVFGVIVYRITTAAALSLNKATRSNVRVTVTATAVIINLVVILILDEIYGAVA KWLTKIEVPKTEQTFEERLILKAFLLKFVNAYSPIFYVAFFKGRFVGRPGSYVYVFDGYR MEECAPGGCLMELCIQLSIIMLGKQLIQNNIFEIGVPKLKKLFRKLKDETEAGETDSAHS KHPEQWDLDYSLEPYTGLTPEYMEMIIQFGFVTLFVASFPLAPVFALLNNVIEVRLDAKK FVTELRRPDAVRTKDIGIWFDILSGIGKFSVISNAFVIAITSDFIPRLVYQYSYSHNGTL HGFVNHTLSFFNVSQLKEGTQPENSQFDQEVQFCRFKDYREPPWAPNPYEFSKQYWFILS ARLAFVIIFQNLVMFLSVLVDWMIPDIPTDISDQIKKEKSLLVDFFLKEEHEKLKLMDEP ALRSPGGGDRSRSRAASSAPSGQSQLGSMMSSGSQHTNV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3072
Start on reference sequence
73
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
