Gene:
Gene Symbol:
SLC9A7
Gene Name:
solute carrier family 9, subfamily A (NHE7, cation proton antiporter 7), member 7
Original Clone ID:
FLH269905.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297137
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGCCTGGTGACGCGGCGCGCCCTGGCTCGGGTCGGGCTACCGGGGCGCCGCCGCCG
CGGCTGCTGCTGCTGCCGCTGCTGCTGGGTTGGGGGCTGCGAGTCGCGGCCGCGGCCTCG
GCCTCCTCCTCTGGGGCGGCGGCGGAGGACAGCAGCGCCATGGAGGAGCTCGCTACTGAG
AAGGAGGCGGAGGAGAGCCACCGGCAAGACAGCGTGAGCCTGCTCACCTTCATCCTGCTG
CTCACGCTCACCATCCTCACCATCTGGCTCTTCAAGCACCGCCGGGTGCGCTTTCTGCAC
GAGACCGGGCTGGCCATGATCTATGGGCTCATCGTTGGGGTGATCCTGAGGTATGGTACC
CCTGCTACCAGTGGCCGTGACAAATCACTCAGCTGCACTCAGGAAGACAGGGCCTTCAGT
ACCTTATTAGTGAATGTCAGCGGAAAGTTCTTCGAATACACTCTGAAAGGAGAAATCAGT
CCTGGCAAGATCAACAGCGTAGAGCAGAATGATATGCTACGGAAGGTAACATTCGATCCA
GAAGTATTTTTCAACATTCTTCTGCCTCCAATTATTTTTCATGCTGGATACAGCTTAAAG
AAGAGACACTTTTTCAGAAATCTTGGATCTATACTGGCCTATGCCTTCTTGGGGACTGCT
GTTTCATGCTTCATTATTGGAAATCTCATGTATGGTGTGGTGAAGCTCATGAAGATTATG
GGACAGCTCTCAGATAAATTTTACTACACAGATTGTCTCTTTTTTGGAGCAATCATCTCT
GCCACTGACCCAGTGACTGTGCTGGCGATATTTAATGAATTGCATGCAGACGTGGATCTT
TACGCACTTCTTTTTGGAGAGAGCGTCCTAAATGATGCTGTTGCCATTGTACTGTCCTCG
TCTATTGTTGCCTACCAGCCAGCGGGACTGAACACTCACGCCTTTGATGCTGCTGCCTTT
TTTAAGTCAGTTGGCATTTTTCTAGGTATATTTAGTGGCTCTTTTACCATGGGAGCTGTG
ACTGGTGTTAATGCCAACGTGACTAAGTTTACCAAACTGCACTGCTTCCCCCTGCTGGAG
ACGGCGCTGTTCTTCCTCATGTCCTGGAGCACGTTTCTCTTGGCAGAAGCCTGCGGATTT
ACAGGTGTTGTAGCTGTCCTTTTCTGTGGAATCACACAAGCTCATTACACCTACAACAAT
CTGTCGGTGGAATCAAGAAGTCGAACCAAGCAGCTCTTTGAGGTGTTACATTTCCTGGCA
GAGAACTTCATCTTCTCCTACATGGGCCTGGCACTGTTTACCTTCCAGAAGCACGTTTTC
AGCCCCATTTTCATCATCGGAGCTTTTGTTGCCATCTTCCTGGGCAGAGCCGCGCACATC
TACCCGCTCTCCTTCTTCCTCAACTTGGGCAGAAGGCATAAGATTGGCTGGAATTTTCAA
CACATGATGATGTTTTCAGGCCTCAGGGGAGCAATGGCATTTGCGTTGGCCATCCGTGAC
ACGGCATCCTATGCTCGCCAGATGATGTTCACGACCACCCTTCTCATTGTGTTCTTCACT
GTCTGGATCATTGGAGGAGGCACGACACCCATGTTGTCATGGCTTAACATCAGAGTTGGC
GTCGAGGAGCCCTCCGAAGAGGACCAGAATGAACACCACTGGCAGTACTTCAGAGTTGGT
GTTGACCCCGATCAAGACCCACCACCCAACAACGACAGCTTTCAAGTCTTACAAGGGGAC
GGCCCAGATTCTGCCAGAGGAAACCGGACAAAACAGGAGAGCGCATGGATATTCAGGCTG
TGGTACAGCTTTGATCACAATTACCTGAAGCCCATCCTCACACACAGTGGTCCCCCACTA
ACCACCACGCTCCCCGCCTGGTGTGGCTTACTAGCTCGATGTCTGACCAGTCCCCAGGTG
TACGATAACCAAGAGCCACTGAGAGAGGAAGACTCTGATTTCATCCTGACCGAAGGCGAC
CTGACATTGACCTACGGGGACAGCACAGTGACTGCAAATGGCTCCTCAAGTTCGCACACC
GCCTCCACGAGTCTGGAGGGCAGCCGGAGAACGAAGAGCAGCTCGGAGGAAGTGCTGGAG
CGAGACCTGGGAATGGGAGACCAGAAGGTTTCGAGCCGGGGCACCCGCCTAGTGTTTCCC
CTGGAAGATAATGCT
Protein sequence predicted based on nucleotide sequence
MEPGDAARPGSGRATGAPPPRLLLLPLLLGWGLRVAAAASASSSGAAAEDSSAMEELATE KEAEESHRQDSVSLLTFILLLTLTILTIWLFKHRRVRFLHETGLAMIYGLIVGVILRYGT PATSGRDKSLSCTQEDRAFSTLLVNVSGKFFEYTLKGEISPGKINSVEQNDMLRKVTFDP EVFFNILLPPIIFHAGYSLKKRHFFRNLGSILAYAFLGTAVSCFIIGNLMYGVVKLMKIM GQLSDKFYYTDCLFFGAIISATDPVTVLAIFNELHADVDLYALLFGESVLNDAVAIVLSS SIVAYQPAGLNTHAFDAAAFFKSVGIFLGIFSGSFTMGAVTGVNANVTKFTKLHCFPLLE TALFFLMSWSTFLLAEACGFTGVVAVLFCGITQAHYTYNNLSVESRSRTKQLFEVLHFLA ENFIFSYMGLALFTFQKHVFSPIFIIGAFVAIFLGRAAHIYPLSFFLNLGRRHKIGWNFQ HMMMFSGLRGAMAFALAIRDTASYARQMMFTTTLLIVFFTVWIIGGGTTPMLSWLNIRVG VEEPSEEDQNEHHWQYFRVGVDPDQDPPPNNDSFQVLQGDGPDSARGNRTKQESAWIFRL WYSFDHNYLKPILTHSGPPLTTTLPAWCGLLARCLTSPQVYDNQEPLREEDSDFILTEGD LTLTYGDSTVTANGSSSSHTASTSLEGSRRTKSSSEEVLERDLGMGDQKVSSRGTRLVFP LEDNA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2186
Start on reference sequence
9
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
