Gene:
Gene Symbol:
Hira
Gene Name:
histone cell cycle regulation defective homolog A (S. cerevisiae)
Original Clone ID:
FLH270135.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297272
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3045nts
Open reading frame: 1 to 3045
ATGAAGCTCTTGAAGCCAACCTGGGTCAACCACAATGGGAAGCCAATTTTTTCAGTTGAT
ATTCACCCTGATGGGACCAAGTTTGCAACTGGAGGACAAGGGCAGGATTCTGGGAAGGTT
GTGATCTGGAATATGTCTCCAGTCCTCCAGGAGGATGACGAGAAGGATGAAAATATTCCC
AAGATGCTTTGCCAGATGGACAATCACTTAGCATGTGTGAACTGTGTGCGGTGGTCAAAC
AGTGGGATGTATTTAGCTTCTGGGGGAGATGACAAACTGATTATGGTGTGGAAGCGGGCT
ACGTACATTGGGCCCAGCACTGTGTTTGGTTCCAGTGGTAAGCTTGCCAATGTGGAGCAA
TGGCGGTGTGTCTCCATCCTCCGGAGTCACTCAGGCGATGTGATGGATGTAGCATGGTCT
CCCCACGACGCCTGGCTGGCCTCATGCAGCGTGGATAACACTGTTGTCATTTGGAATGCC
GTGAAGTTCCCAGAAATTCTTGCAACTCTGAGAGGTCATTCTGGCTTAGTAAAAGGTTTG
ACTTGGGATCCCGTTGGTAAATATATTGCCTCTCAAGCTGATGATCGAAGTTTGAAGGTA
TGGAGGACGCTGGACTGGCAGCTAGAGACTAGCATCACCAAGCCTTTTGATGAGTGTGGA
GGAACGACGCATGTTCTCCGGCTTAGTTGGTCACCTGATGGCCATTACCTGGTATCTGCC
CATGCCATGAATAATTCTGGCCCCACTGCTCAGATCATCGAAAGAGAGGGCTGGAAGACC
AACATGGACTTTGTGGGTCACCGGAAAGCTGTGACTGTTGTGAAATTCAACCCAAAAATC
TTCAAGAAGAAGCAGAAGAATGGGAGCTCTACAAAGCCCAGCTGCCCATACTGCTGCTGT
GCTGTTGGCAGCAAGGACCGCTCACTCTCTGTCTGGCTCACATGTTTGAAACGGCCTCTG
GTTGTCATCCATGAACTGTTTGACAAGTCCATCATGGATATTTCCTGGACTCTGAATGGG
TTGGGTATCCTGGTGTGCTCCATGGACGGCTCTGTGGCGTTCCTTGATTTCTCTCAGGAT
GAACTCGGAGACCCCCTGAGTGAGGAGGAAAAGAGCCGAATTCACCAGTCTACCTATGGC
AAGAGCCTGGCAATAATGACTGAGGCCCAGCTTTCCACAGCTGTTATTGAGAACCCTGAG
ATGCTCAAGTACCAGCGGAGACAGCAACAGCAGCAGCTGGATCAGAAGAATGCCACTACT
AGGGAGACAAGCTCAGCATCCTCAGTCACGGGTGTGGTCAATGGGGAAAGTCTAGAAGAT
ATCAGAAAGAATCTTTTGAAGAAACAAGTGGAAACTCGGACAGCAGATGGTCGGAGGAGA
ATCACGCCTCTTTGCATAGCACAGCTGGACACTGGGGACTTCTCCACGGCATTCTTCAAC
AGCATCCCACTCTCCAGCTCCCTAGCAGGCACCATGCTCTCCTCTCCTAGTGGTCAGCAG
CTACTACCACTGGACTCCAGTACCCCCTCCTTCGGCGCCTCAAAGCCTTGCACAGAACCA
GTGGCAGCCACCAGTGCCAGGCCTACAGGCGAATCTGTCAGTAAGGACAGTATGAATGCT
ACCTCTACTCCTGCTGCATCGTCACCCTCTGTGTTAACAACCCCATCCAAGATTGAACCC
ATGAAAGCATTTGATTCCCGGTTCACAGAACGGTCCAAAGCCACACCAGGTGCTCCTTCC
TTGACCAGTGTGATTCCAACAGCTGTTGAAAGGTTGAAAGAGCAGAACCTCGTCAAGGAG
CTGAGGTCCCGGGAACTGGAGAGCAGCAGTGACAGCGATGAGAAGGTCCACCTAGCCAAG
CCCTCTTCACTGTCCAAGCGCAAACTTGAGCTTGAGGTAGAGACGGTGGAAAAGAAGAAG
AAAGGTCGCCCTAGGAAGGATTCACGTCTTTTACCCATGTCTCTGTCCGTCCAGTCTCCA
GCTGCCCTGTCTACAGAGAAGGAGGCCATGTGTCTGTCTGCACCAGCACTTGCACTGAAG
CTGCCAATTCCAGGCCCACAGAGAGCGTTCACCCTCCAGGTGAGCTCTGACCCCTCCATG
TACATTGAGGTGGAGAATGAAGTGACCACCGTTGGGGGGATAAGGCTGAGTCGCCTGAAG
TGCAACCGTGAAGGGAAGGAGTGGGAGACAGTGCTCAGCAGTCGCGTCCTCACTGCTGCC
GGCAGCTGTGATGTGGTATGTGTTGCCTGTGAAAAGAGGATGCTGTCGGTGTTCTCTACC
TGTGGTCGCCGTCTCCTCCCTCCCATCCTCCTTCCATCTCCAATCTCTACTTTGCACTGC
ACGGGCCCCTACGTCATGGCACTCACCGCTGCAGCCACACTGTCTGTCTGGGATGTTCAC
AGACAGGTGGTTGTGGTGAAAGAAGAATCTCTACACTCCATCTTGTCAGGAAGTGATATG
ACGGTGTCACAGATCTTGCTAACACAGCATGGAATCCCAGTGATGAATTTGTCTGATGGG
AAAGCATACTGCTTTAATCCATCCCTCTCCACATGGAACCTGGTTTCTGACAAGCAAGAT
TCATTGGCCCAGTGTGCAGACTTCAGGAACAGCCTGCCGTCCCAGGATGCCATGCTTTGT
TCAGGACCGTTAGCCATAATTCAGGGCCGCACCTCCAACTCTGGAAGGCAAGCTGCCCGG
CTCTTCTCCGTGCCTCATGTGGTACAGCAGGAGACCACACTGGCCTACCTAGAGAACCAG
GTTGCTGCGGCACTTACCCTGCAGTCAAGCCACGAGTATCGTCACTGGCTCCTCCTTTAT
GCTCGGTACCTTGTGAATGAAGGGTTTGAATACCGCCTCCGTGAAATATGCAAGGACTTG
CTGGGTCCAGTTCACTGCTCCACTGGAAGTCAGTGGGAGTCAACAGTAGTGGGTCTGCGG
AAAAGGGAGCTGCTGAAGGAACTGTTGCCAGTCATTGGGCAGAATCTTCGATTCCAGCGC
CTCTTTACTGAGTGCCAGGAACAACTGGACATCCTGAGAGACAAG
Protein sequence predicted based on nucleotide sequence
MKLLKPTWVNHNGKPIFSVDIHPDGTKFATGGQGQDSGKVVIWNMSPVLQEDDEKDENIP KMLCQMDNHLACVNCVRWSNSGMYLASGGDDKLIMVWKRATYIGPSTVFGSSGKLANVEQ WRCVSILRSHSGDVMDVAWSPHDAWLASCSVDNTVVIWNAVKFPEILATLRGHSGLVKGL TWDPVGKYIASQADDRSLKVWRTLDWQLETSITKPFDECGGTTHVLRLSWSPDGHYLVSA HAMNNSGPTAQIIEREGWKTNMDFVGHRKAVTVVKFNPKIFKKKQKNGSSTKPSCPYCCC AVGSKDRSLSVWLTCLKRPLVVIHELFDKSIMDISWTLNGLGILVCSMDGSVAFLDFSQD ELGDPLSEEEKSRIHQSTYGKSLAIMTEAQLSTAVIENPEMLKYQRRQQQQQLDQKNATT RETSSASSVTGVVNGESLEDIRKNLLKKQVETRTADGRRRITPLCIAQLDTGDFSTAFFN SIPLSSSLAGTMLSSPSGQQLLPLDSSTPSFGASKPCTEPVAATSARPTGESVSKDSMNA TSTPAASSPSVLTTPSKIEPMKAFDSRFTERSKATPGAPSLTSVIPTAVERLKEQNLVKE LRSRELESSSDSDEKVHLAKPSSLSKRKLELEVETVEKKKKGRPRKDSRLLPMSLSVQSP AALSTEKEAMCLSAPALALKLPIPGPQRAFTLQVSSDPSMYIEVENEVTTVGGIRLSRLK CNREGKEWETVLSSRVLTAAGSCDVVCVACEKRMLSVFSTCGRRLLPPILLPSPISTLHC TGPYVMALTAAATLSVWDVHRQVVVVKEESLHSILSGSDMTVSQILLTQHGIPVMNLSDG KAYCFNPSLSTWNLVSDKQDSLAQCADFRNSLPSQDAMLCSGPLAIIQGRTSNSGRQAAR LFSVPHVVQQETTLAYLENQVAAALTLQSSHEYRHWLLLYARYLVNEGFEYRLREICKDL LGPVHCSTGSQWESTVVGLRKRELLKELLPVIGQNLRFQRLFTECQEQLDILRDK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3781
Start on reference sequence
734
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
