DNASU Plasmid | Ikzf4 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Ikzf4

Gene Name: IKAROS family zinc finger 4

Original Clone ID:

FLH481767.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00297448

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1761nts

Open reading frame: 35 to 1795

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGCACACACCACCCGCACTCCCTCG
CCGTTTCCAAGGCGGCGGCCGCGTTCGCACCCCAGGGTCTCACCGGCAAGGGAAGGATAA
TCTGGAGAGGGAGCTCTCAGGAGGGTGTGCTCCGGATTTCTTGCCTCAGGCCCAGGACTC
CAACCATTTTATAATGGAATCTTTATTTTGTGAAAGTAGCGGGGACTCATCTCTGGAGAA
GGAGTTCCTTGGGGCCCCAGTGGGGCCCTCGGTGAGCACCCCAAACAGCCAACACTCTTC
ACCCAGCCGCTCGCTCAGTGCCAACTCCATCAAGGTGGAGATGTACAGCGATGAGGAGTC
GAGCAGACTGCTGGGGCCGGATGAACGGCTCCTGGATAAGGATGACAGTGTGATTGTGGA
AGACTCATTGTCAGAGCCCTTAGGCTACTGCGATGGAAGTGGGCCAGAGCCTCACTCCCC
TGGCGGCATCCGGCTACCCAACGGCAAGCTCAAGTGCGACGTCTGCGGCATGGTCTGCAT
TGGGCCCAATGTGCTCATGGTACACAAGCGCAGCCACACTGGGGAGAGGCCCTTCCACTG
TAATCAGTGTGGTGCCTCCTTCACACAGAAGGGCAATCTGCTTCGCCACATCAAGCTGCA
CTCGGGGGAGAAGCCCTTCAAGTGCCCCTTCTGCAACTATGCCTGCCGCCGGCGTGACGC
ACTCACTGGCCACCTCCGCACACACTCAGTCTCCTCCCCCACCGTGGGCAAACCCTACAA
GTGCAACTACTGTGGCCGGAGCTACAAACAGCAAAGTACCCTGGAGGAGCACAAGGAGAG
GTGCCACAACTACCTACAGAGTCTCAGCACTGATGCCCAAGCTCTGACTGGCCAGCCAGG
TGATGAAATCCGTGACCTGGAGATGGTGCCTGACTCAATGCTGCACCCATCGACTGAACG
GCCAACTTTCATTGATCGTTTGGCCAACAGCCTCACCAAACGCAAGCGTTCCACCCCACA
GAAGTTTGTAGGTGAAAAGCAGATGCGCTTCAGCCTCTCAGACCTTCCCTATGATGTGAA
TGCCAGCGGTGGCTATGAAAAGGACGTAGAGTTGGTGGCACACCATGGCCTGGAGCCTGG
CTTTGGAGGGTCTCTAGCCTTTGTGGGTACAGAGCATCTGCGTCCCCTCCGCCTCCCACC
CACCAACTGCATCTCAGAACTCACACCTGTCATCAGCTCTGTGTACACCCAAATGCAGCC
CATCCCCAGCCGACTGGAGCTTCCAGGGTCCCGAGAAGCAGGTGAGGGACCGGAGGACCT
GGGAGATGGAGGTCCCCTCCTTTATCGGGCCCGAGGCTCTCTGACTGACCCTGGGGCATC
CCCCAGCAATGGCTGCCAGGACTCCACAGATACAGAGAGCAACCACGAAGACCGGATTGG
TGGGGTGGTATCCCTTCCTCAGGGTCCCCCACCCCAACCTCCTCCCACCATAGTGGTGGG
CCGGCACAGTCCCGCCTATGCCAAAGAGGACCCCAAACCACAGGAGGGGTTACTGCGGGG
CACCCCAGGCCCCTCCAAGGAAGTGCTTCGGGTGGTGGGTGAGAGTGGTGAGCCAGTGAA
GGCCTTTAAGTGTGAACACTGCCGCATCCTCTTTCTGGACCACGTCATGTTCACCATCCA
CATGGGCTGCCACGGCTTCAGAGACCCTTTTGAGTGTAACATCTGTGGTTATCACAGCCA
GGATCGGTATGAGTTCTCTTCCCACATCGTCCGGGGGGAACATAAGGTGGGCTAGGGCCT
CATGGGCCCAGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MHTPPALPRRFQGGGRVRTPGSHRQGKDNLERELSGGCAPDFLPQAQDSNHFIMESLFCE
SSGDSSLEKEFLGAPVGPSVSTPNSQHSSPSRSLSANSIKVEMYSDEESSRLLGPDERLL
DKDDSVIVEDSLSEPLGYCDGSGPEPHSPGGIRLPNGKLKCDVCGMVCIGPNVLMVHKRS
HTGERPFHCNQCGASFTQKGNLLRHIKLHSGEKPFKCPFCNYACRRRDALTGHLRTHSVS
SPTVGKPYKCNYCGRSYKQQSTLEEHKERCHNYLQSLSTDAQALTGQPGDEIRDLEMVPD
SMLHPSTERPTFIDRLANSLTKRKRSTPQKFVGEKQMRFSLSDLPYDVNASGGYEKDVEL
VAHHGLEPGFGGSLAFVGTEHLRPLRLPPTNCISELTPVISSVYTQMQPIPSRLELPGSR
EAGEGPEDLGDGGPLLYRARGSLTDPGASPSNGCQDSTDTESNHEDRIGGVVSLPQGPPP
QPPPTIVVGRHSPAYAKEDPKPQEGLLRGTPGPSKEVLRVVGESGEPVKAFKCEHCRILF
LDHVMFTIHMGCHGFRDPFECNICGYHSQDRYEFSSHIVRGEHKVG*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 187

End on reference sequence 1947

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Ikzf4

Symbol Nomenclature :

Ikzf4

SYNONYM :

A630026H08Rik; Eos; Zfpn1a4; Znfn1a4

Designation :

ikaros family zinc finger protein 4|zinc finger protein Eos|zinc finger protein, subfamily 1A, 4 (Eos)

Full Nomenclature :

IKAROS family zinc finger 4

GENEID :

22781

GI :

182888504

GenBank Accession :

BC160352

Mouse Genome Informatics :

1343139

Vega :

OTTMUSG00000025110

Target GenBank:

NM_011772.2

Protein

SMART domain :

ZnF_C2H2 : zinc finger

UniProt :

Q8C208

Clone

Cloning Information :

481767

HIP Master Clone ID :

399495

IMAGE ID :

100064096

Original Clone ID :

FLH481767.01X

Organism

Species Specific ID:

22781

Genome

Chromosome :

Map Location :

10 D3|10

Ensembl :

ENSMUSG00000002578

Reagents

Labome :

Eos-antibody