DNASU Plasmid | PSMD2 (H. sapiens )<br>In pLDNT7_nFLAG (Flag-tagged<br>In vitro expression vector)

Explanation of Terms

Gene:

Gene Symbol: PSMD2

Gene Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 2

Original Clone ID:

FLH230518.01L

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pLDNT7_nFLAG

Format:

FUSION

Source:

HIP

Description:

None

Comments:

This clone is identical to HsCD00616892

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

HsCD00298487

Price:

UNAVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2727nts

Open reading frame: 1 to 2727

Reference Sequence Alignment

ATGGAGGAGGGAGGCCGGGACAAGGCGCCGGTGCAGCCCCAGCAGTCTCCAGCGGCGGCC
CCCGGCGGCACGGACGAGAAGCCGAGCGGCAAGGAGCGGCGGGATGCCGGGGACAAGGAC
AAAGAACAGGAGCTGTCTGAAGAGGATAAACAGCTTCAAGATGAACTGGAGATGCTCGTG
GAACGACTAGGGGAGAAGGATACATCCCTGTATCGACCAGCGCTGGAGGAATTGCGAAGG
CAGATTCGTTCTTCTACAACTTCCATGACTTCAGTGCCCAAGCCTCTCAAATTTCTGCGT
CCACACTATGGCAAACTGAAGGAAATCTATGAGAACATGGCCCCTGGGGAGAATAAGCGT
TTTGCTGCTGACATCATCTCCGTTTTGGCCATGACCATGAGTGGGGAGCGTGAGTGCCTC
AAGTATCGGCTAGTGGGCTCCCAGGAGGAATTGGCATCATGGGGTCATGAGTATGTCAGG
CATCTGGCAGGAGAAGTGGCTAAGGAGTGGCAGGAGCTGGATGACGCAGAGAAGGTCCAG
CGGGAGCCTCTGCTCACTCTGGTGAAGGAAATCGTCCCCTATAACATGGCCCACAATGCA
GAGCATGAGGCTTGCGACCTGCTTATGGAAATTGAGCAGGTGGACATGCTGGAGAAGGAC
ATTGATGAAAATGCATATGCAAAGGTCTGCCTTTATCTCACCAGTTGTGTGAATTACGTG
CCTGAGCCTGAGAACTCAGCCCTACTGCGTTGTGCCCTGGGTGTGTTCCGAAAGTTTAGC
CGCTTCCCTGAAGCTCTGAGATTGGCATTGATGCTCAATGACATGGAGTTGGTAGAAGAC
ATCTTCACCTCCTGCAAGGATGTGGTAGTACAGAAACAGATGGCATTCATGCTAGGCCGG
CATGGGGTGTTCCTGGAGCTGAGTGAAGATGTCGAGGAGTATGAGGACCTGACAGAGATC
ATGTCCAATGTACAGCTCAACAGCAACTTCTTGGCCTTAGCTCGGGAGCTGGACATCATG
GAGCCCAAGGTGCCTGATGACATCTACAAAACCCACCTAGAGAACAACAGGTTTGGGGGC
AGTGGCTCTCAGGTGGACTCTGCCCGCATGAACCTGGCCTCCTCTTTTGTGAATGGCTTT
GTGAATGCAGCTTTTGGCCAAGACAAGCTGCTAACAGATGATGGCAACAAATGGCTTTAC
AAGAACAAGGACCACGGAATGTTGAGTGCAGCTGCATCTCTTGGGATGATTCTGCTGTGG
GATGTGGATGGTGGCCTCACCCAGATTGACAAGTACCTGTACTCCTCTGAGGACTACATT
AAGTCAGGAGCTCTTCTTGCCTGTGGCATAGTGAACTCTGGGGTCCGGAATGAGTGTGAC
CCTGCTCTGGCACTGCTCTCAGACTATGTTCTCCACAACAGCAACACCATGAGACTTGGT
TCCATCTTTGGGCTAGGCTTGGCTTATGCTGGCTCAAATCGTGAAGATGTCCTAACACTG
CTGCTGCCTGTGATGGGAGATTCAAAGTCCAGCATGGAGGTGGCAGGTGTCACAGCTTTA
GCCTGTGGAATGATAGCAGTAGGGTCCTGCAATGGAGATGTAACTTCCACTATCCTTCAG
ACCATCATGGAGAAGTCAGAGACTGAGCTCAAGGATACTTATGCTCGTTGGCTTCCTCTT
GGACTGGGTCTCAACCACCTGGGGAAGGGTGAGGCCATCGAGGCAATCCTGGCTGCACTG
GAGGTTGTGTCAGAGCCATTCCGCAGTTTTGCCAACACACTGGTGGATGTGTGTGCATAT
GCAGGCTCTGGGAATGTGCTGAAGGTGCAGCAGCTGCTCCACATTTGTAGCGAACACTTT
GACTCCAAAGAGAAGGAGGAAGACAAAGACAAGAAGGAAAAGAAAGACAAGGACAAGAAG
GAAGCCCCTGCTGACATGGGAGCACATCAGGGAGTGGCTGTTCTGGGGATTGCCCTTATT
GCTATGGGGGAGGAGATTGGTGCAGAGATGGCATTACGAACCTTTGGCCACTTGCTGAGA
TATGGGGAGCCTACACTCCGGAGGGCTGTACCTTTAGCACTGGCCCTCATCTCTGTTTCA
AATCCACGACTCAACATCCTGGATACCCTAAGCAAATTCTCTCATGATGCTGATCCAGAA
GTTTCCTATTACTCCATTTTTGCCATGGGCATGGTGGGCAGTGGTACCAATAATGCCCGT
CTGGCTGCAATGCTGCGCCAGTTAGCTCAATATCATGCCAAGGACCCAAACAACCTCTTC
ATGGTGCGCTTGGCACAGGGCCTGACACATTTAGGGAAGGGCACCCTTACCCTCTGCCCC
TACCACAGCGACCGGCAGCTTATGAGCCAGGTGGCCGTGGCTGGACTGCTCACTGTGCTT
GTCTCTTTCCTGGATGTTCGAAACATTATTCTAGGCAAATCACACTATGTATTGTATGGG
CTGGTGGCTGCCATGCAGCCCCGAATGCTGGTTACGTTTGATGAGGAGCTGCGGCCATTG
CCAGTGTCTGTCCGTGTGGGCCAGGCAGTGGATGTGGTGGGCCAGGCTGGCAAGCCGAAG
ACTATCACAGGGTTCCAGACGCATACAACCCCAGTGTTGTTGGCCCACGGGGAACGGGCA
GAATTGGCCACTGAGGAGTTTCTTCCTGTTACCCCCATTCTGGAAGGTTTTGTTATCCTT
CGGAAGAACCCCAATTATGATCTCTTG

Protein sequence predicted based on nucleotide sequence

MEEGGRDKAPVQPQQSPAAAPGGTDEKPSGKERRDAGDKDKEQELSEEDKQLQDELEMLV
ERLGEKDTSLYRPALEELRRQIRSSTTSMTSVPKPLKFLRPHYGKLKEIYENMAPGENKR
FAADIISVLAMTMSGERECLKYRLVGSQEELASWGHEYVRHLAGEVAKEWQELDDAEKVQ
REPLLTLVKEIVPYNMAHNAEHEACDLLMEIEQVDMLEKDIDENAYAKVCLYLTSCVNYV
PEPENSALLRCALGVFRKFSRFPEALRLALMLNDMELVEDIFTSCKDVVVQKQMAFMLGR
HGVFLELSEDVEEYEDLTEIMSNVQLNSNFLALARELDIMEPKVPDDIYKTHLENNRFGG
SGSQVDSARMNLASSFVNGFVNAAFGQDKLLTDDGNKWLYKNKDHGMLSAAASLGMILLW
DVDGGLTQIDKYLYSSEDYIKSGALLACGIVNSGVRNECDPALALLSDYVLHNSNTMRLG
SIFGLGLAYAGSNREDVLTLLLPVMGDSKSSMEVAGVTALACGMIAVGSCNGDVTSTILQ
TIMEKSETELKDTYARWLPLGLGLNHLGKGEAIEAILAALEVVSEPFRSFANTLVDVCAY
AGSGNVLKVQQLLHICSEHFDSKEKEEDKDKKEKKDKDKKEAPADMGAHQGVAVLGIALI
AMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVSNPRLNILDTLSKFSHDADPE
VSYYSIFAMGMVGSGTNNARLAAMLRQLAQYHAKDPNNLFMVRLAQGLTHLGKGTLTLCP
YHSDRQLMSQVAVAGLLTVLVSFLDVRNIILGKSHYVLYGLVAAMQPRMLVTFDEELRPL
PVSVRVGQAVDVVGQAGKPKTITGFQTHTTPVLLAHGERAELATEEFLPVTPILEGFVIL
RKNPNYDLL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Insert was fully sequenced in parent vector

Reference Sequence Annotations:

Start on reference sequence 10

End on reference sequence 2736

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol

Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.

Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pLDNT7_nFLAG

Synonyms:

pLDNT7-Flag

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning

Comments:

None

Size (bp):

4557

Parent Vector:

None

Properties:

Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker

Author Name:

Daniel Schiwek

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

GGGCGAATTAATTCCGGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCACCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGATCGGATCCGAATTCGAGCTCCGTCATCAACAAGTTTGTACAAAAAAGCAGGCTCCACCATGGCCGACTACAAGGACCACGACGGCGACTACAAGGACCACGACATCGACTACAAGGACGACGACGACAAGAGATCTATAACTTCGTATAGCATACATTATACGAAGTTATTGATCCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTTTGGACCCAGCTTTCTTGTACAAAGTGGTTGATGACAAGCTTGCGGCCGCACTCGAGCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTTGAAGATCTGACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTTAAACACTAGTCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE1 ori

ColE1 origin of replication

1842

2524

promoter

T7 promoter

4462

4479

recombination site

LoxP

LoxP site for recombinational cloning

657

690

selectable marker

AmpR

ampicillin resistance gene

2622

3281

ssDNA origin

f1 ori

f1 origin for ssDNA production

3928

4368

tag

Flag

3xFlag epitope tag ORF and LoxP region

585

650

trxn termination sequence

T7 term

T7 terminator sequence

1614

1660

Gene

Pathways

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

PSMD2

Symbol Nomenclature :

PSMD2

SYNONYM :

P97; RPN1; S2; TRAP2

Designation :

26S proteasome non-ATPase regulatory subunit 2|55.11 protein|TNFR-associated protein 2|protein 55.11|tumor necrosis factor type 1 receptor-associated protein 2

Full Nomenclature :

proteasome (prosome, macropain) 26S subunit, non-ATPase, 2

GENEID :

5708

SYNONYM :

HsCD00616892

HGNC :

9559

MIM :

606223

Vega :

OTTHUMG00000156796

Target GenBank:

BC007897

Reference Sequence Alignment

  

HsCD00298487      ATGGAGGAGGGAGGCCGGGACAAGGCGCCGGTGCAGCCCCAGCAGTCTCCAGCGGCGGCC
NM_002808.4       ATGGAGGAGGGAGGCCGGGACAAGGCGCCGGTGCAGCCCCAGCAGTCTCCAGCGGCGGCC
                  ************************************************************

HsCD00298487      CCCGGCGGCACGGACGAGAAGCCGAGCGGCAAGGAGCGGCGGGATGCCGGGGACAAGGAC
NM_002808.4       CCCGGCGGCACGGACGAGAAGCCGAGCGGCAAGGAGCGGCGGGATGCCGGGGACAAGGAC
                  ************************************************************

HsCD00298487      AAAGAACAGGAGCTGTCTGAAGAGGATAAACAGCTTCAAGATGAACTGGAGATGCTCGTG
NM_002808.4       AAAGAACAGGAGCTGTCTGAAGAGGATAAACAGCTTCAAGATGAACTGGAGATGCTCGTG
                  ************************************************************

HsCD00298487      GAACGACTAGGGGAGAAGGATACATCCCTGTATCGACCAGCGCTGGAGGAATTGCGAAGG
NM_002808.4       GAACGACTAGGGGAGAAGGATACATCCCTGTATCGACCAGCGCTGGAGGAATTGCGAAGG
                  ************************************************************

HsCD00298487      CAGATTCGTTCTTCTACAACTTCCATGACTTCAGTGCCCAAGCCTCTCAAATTTCTGCGT
NM_002808.4       CAGATTCGTTCTTCTACAACTTCCATGACTTCAGTGCCCAAGCCTCTCAAATTTCTGCGT
                  ************************************************************

HsCD00298487      CCACACTATGGCAAACTGAAGGAAATCTATGAGAACATGGCCCCTGGGGAGAATAAGCGT
NM_002808.4       CCACACTATGGCAAACTGAAGGAAATCTATGAGAACATGGCCCCTGGGGAGAATAAGCGT
                  ************************************************************

HsCD00298487      TTTGCTGCTGACATCATCTCCGTTTTGGCCATGACCATGAGTGGGGAGCGTGAGTGCCTC
NM_002808.4       TTTGCTGCTGACATCATCTCCGTTTTGGCCATGACCATGAGTGGGGAGCGTGAGTGCCTC
                  ************************************************************

HsCD00298487      AAGTATCGGCTAGTGGGCTCCCAGGAGGAATTGGCATCATGGGGTCATGAGTATGTCAGG
NM_002808.4       AAGTATCGGCTAGTGGGCTCCCAGGAGGAATTGGCATCATGGGGTCATGAGTATGTCAGG
                  ************************************************************

HsCD00298487      CATCTGGCAGGAGAAGTGGCTAAGGAGTGGCAGGAGCTGGATGACGCAGAGAAGGTCCAG
NM_002808.4       CATCTGGCAGGAGAAGTGGCTAAGGAGTGGCAGGAGCTGGATGACGCAGAGAAGGTCCAG
                  ************************************************************

HsCD00298487      CGGGAGCCTCTGCTCACTCTGGTGAAGGAAATCGTCCCCTATAACATGGCCCACAATGCA
NM_002808.4       CGGGAGCCTCTGCTCACTCTGGTGAAGGAAATCGTCCCCTATAACATGGCCCACAATGCA
                  ************************************************************

HsCD00298487      GAGCATGAGGCTTGCGACCTGCTTATGGAAATTGAGCAGGTGGACATGCTGGAGAAGGAC
NM_002808.4       GAGCATGAGGCTTGCGACCTGCTTATGGAAATTGAGCAGGTGGACATGCTGGAGAAGGAC
                  ************************************************************

HsCD00298487      ATTGATGAAAATGCATATGCAAAGGTCTGCCTTTATCTCACCAGTTGTGTGAATTACGTG
NM_002808.4       ATTGATGAAAATGCATATGCAAAGGTCTGCCTTTATCTCACCAGTTGTGTGAATTACGTG
                  ************************************************************

HsCD00298487      CCTGAGCCTGAGAACTCAGCCCTACTGCGTTGTGCCCTGGGTGTGTTCCGAAAGTTTAGC
NM_002808.4       CCTGAGCCTGAGAACTCAGCCCTACTGCGTTGTGCCCTGGGTGTGTTCCGAAAGTTTAGC
                  ************************************************************

HsCD00298487      CGCTTCCCTGAAGCTCTGAGATTGGCATTGATGCTCAATGACATGGAGTTGGTAGAAGAC
NM_002808.4       CGCTTCCCTGAAGCTCTGAGATTGGCATTGATGCTCAATGACATGGAGTTGGTAGAAGAC
                  ************************************************************

HsCD00298487      ATCTTCACCTCCTGCAAGGATGTGGTAGTACAGAAACAGATGGCATTCATGCTAGGCCGG
NM_002808.4       ATCTTCACCTCCTGCAAGGATGTGGTAGTACAGAAACAGATGGCATTCATGCTAGGCCGG
                  ************************************************************

HsCD00298487      CATGGGGTGTTCCTGGAGCTGAGTGAAGATGTCGAGGAGTATGAGGACCTGACAGAGATC
NM_002808.4       CATGGGGTGTTCCTGGAGCTGAGTGAAGATGTCGAGGAGTATGAGGACCTGACAGAGATC
                  ************************************************************

HsCD00298487      ATGTCCAATGTACAGCTCAACAGCAACTTCTTGGCCTTAGCTCGGGAGCTGGACATCATG
NM_002808.4       ATGTCCAATGTACAGCTCAACAGCAACTTCTTGGCCTTAGCTCGGGAGCTGGACATCATG
                  ************************************************************

HsCD00298487      GAGCCCAAGGTGCCTGATGACATCTACAAAACCCACCTAGAGAACAACAGGTTTGGGGGC
NM_002808.4       GAGCCCAAGGTGCCTGATGACATCTACAAAACCCACCTAGAGAACAACAGGTTTGGGGGC
                  ************************************************************

HsCD00298487      AGTGGCTCTCAGGTGGACTCTGCCCGCATGAACCTGGCCTCCTCTTTTGTGAATGGCTTT
NM_002808.4       AGTGGCTCTCAGGTGGACTCTGCCCGCATGAACCTGGCCTCCTCTTTTGTGAATGGCTTT
                  ************************************************************

HsCD00298487      GTGAATGCAGCTTTTGGCCAAGACAAGCTGCTAACAGATGATGGCAACAAATGGCTTTAC
NM_002808.4       GTGAATGCAGCTTTTGGCCAAGACAAGCTGCTAACAGATGATGGCAACAAATGGCTTTAC
                  ************************************************************

HsCD00298487      AAGAACAAGGACCACGGAATGTTGAGTGCAGCTGCATCTCTTGGGATGATTCTGCTGTGG
NM_002808.4       AAGAACAAGGACCACGGAATGTTGAGTGCAGCTGCATCTCTTGGGATGATTCTGCTGTGG
                  ************************************************************

HsCD00298487      GATGTGGATGGTGGCCTCACCCAGATTGACAAGTACCTGTACTCCTCTGAGGACTACATT
NM_002808.4       GATGTGGATGGTGGCCTCACCCAGATTGACAAGTACCTGTACTCCTCTGAGGACTACATT
                  ************************************************************

HsCD00298487      AAGTCAGGAGCTCTTCTTGCCTGTGGCATAGTGAACTCTGGGGTCCGGAATGAGTGTGAC
NM_002808.4       AAGTCAGGAGCTCTTCTTGCCTGTGGCATAGTGAACTCTGGGGTCCGGAATGAGTGTGAC
                  ************************************************************

HsCD00298487      CCTGCTCTGGCACTGCTCTCAGACTATGTTCTCCACAACAGCAACACCATGAGACTTGGT
NM_002808.4       CCTGCTCTGGCACTGCTCTCAGACTATGTTCTCCACAACAGCAACACCATGAGACTTGGT
                  ************************************************************

HsCD00298487      TCCATCTTTGGGCTAGGCTTGGCTTATGCTGGCTCAAATCGTGAAGATGTCCTAACACTG
NM_002808.4       TCCATCTTTGGGCTAGGCTTGGCTTATGCTGGCTCAAATCGTGAAGATGTCCTAACACTG
                  ************************************************************

HsCD00298487      CTGCTGCCTGTGATGGGAGATTCAAAGTCCAGCATGGAGGTGGCAGGTGTCACAGCTTTA
NM_002808.4       CTGCTGCCTGTGATGGGAGATTCAAAGTCCAGCATGGAGGTGGCAGGTGTCACAGCTTTA
                  ************************************************************

HsCD00298487      GCCTGTGGAATGATAGCAGTAGGGTCCTGCAATGGAGATGTAACTTCCACTATCCTTCAG
NM_002808.4       GCCTGTGGAATGATAGCAGTAGGGTCCTGCAATGGAGATGTAACTTCCACTATCCTTCAG
                  ************************************************************

HsCD00298487      ACCATCATGGAGAAGTCAGAGACTGAGCTCAAGGATACTTATGCTCGTTGGCTTCCTCTT
NM_002808.4       ACCATCATGGAGAAGTCAGAGACTGAGCTCAAGGATACTTATGCTCGTTGGCTTCCTCTT
                  ************************************************************

HsCD00298487      GGACTGGGTCTCAACCACCTGGGGAAGGGTGAGGCCATCGAGGCAATCCTGGCTGCACTG
NM_002808.4       GGACTGGGTCTCAACCACCTGGGGAAGGGTGAGGCCATCGAGGCAATCCTGGCTGCACTG
                  ************************************************************

HsCD00298487      GAGGTTGTGTCAGAGCCATTCCGCAGTTTTGCCAACACACTGGTGGATGTGTGTGCATAT
NM_002808.4       GAGGTTGTGTCAGAGCCATTCCGCAGTTTTGCCAACACACTGGTGGATGTGTGTGCATAT
                  ************************************************************

HsCD00298487      GCAGGCTCTGGGAATGTGCTGAAGGTGCAGCAGCTGCTCCACATTTGTAGCGAACACTTT
NM_002808.4       GCAGGCTCTGGGAATGTGCTGAAGGTGCAGCAGCTGCTCCACATTTGTAGCGAACACTTT
                  ************************************************************

HsCD00298487      GACTCCAAAGAGAAGGAGGAAGACAAAGACAAGAAGGAAAAGAAAGACAAGGACAAGAAG
NM_002808.4       GACTCCAAAGAGAAGGAGGAAGACAAAGACAAGAAGGAAAAGAAAGACAAGGACAAGAAG
                  ************************************************************

HsCD00298487      GAAGCCCCTGCTGACATGGGAGCACATCAGGGAGTGGCTGTTCTGGGGATTGCCCTTATT
NM_002808.4       GAAGCCCCTGCTGACATGGGAGCACATCAGGGAGTGGCTGTTCTGGGGATTGCCCTTATT
                  ************************************************************

HsCD00298487      GCTATGGGGGAGGAGATTGGTGCAGAGATGGCATTACGAACCTTTGGCCACTTGCTGAGA
NM_002808.4       GCTATGGGGGAGGAGATTGGTGCAGAGATGGCATTACGAACCTTTGGCCACTTGCTGAGA
                  ************************************************************

HsCD00298487      TATGGGGAGCCTACACTCCGGAGGGCTGTACCTTTAGCACTGGCCCTCATCTCTGTTTCA
NM_002808.4       TATGGGGAGCCTACACTCCGGAGGGCTGTACCTTTAGCACTGGCCCTCATCTCTGTTTCA
                  ************************************************************

HsCD00298487      AATCCACGACTCAACATCCTGGATACCCTAAGCAAATTCTCTCATGATGCTGATCCAGAA
NM_002808.4       AATCCACGACTCAACATCCTGGATACCCTAAGCAAATTCTCTCATGATGCTGATCCAGAA
                  ************************************************************

HsCD00298487      GTTTCCTATTACTCCATTTTTGCCATGGGCATGGTGGGCAGTGGTACCAATAATGCCCGT
NM_002808.4       GTTTCCTATAACTCCATTTTTGCCATGGGCATGGTGGGCAGTGGTACCAATAATGCCCGT
                  *********:**************************************************

HsCD00298487      CTGGCTGCAATGCTGCGCCAGTTAGCTCAATATCATGCCAAGGACCCAAACAACCTCTTC
NM_002808.4       CTGGCTGCAATGCTGCGCCAGTTAGCTCAATATCATGCCAAGGACCCAAACAACCTCTTC
                  ************************************************************

HsCD00298487      ATGGTGCGCTTGGCACAGGGCCTGACACATTTAGGGAAGGGCACCCTTACCCTCTGCCCC
NM_002808.4       ATGGTGCGCTTGGCACAGGGCCTGACACATTTAGGGAAGGGCACCCTTACCCTCTGCCCC
                  ************************************************************

HsCD00298487      TACCACAGCGACCGGCAGCTTATGAGCCAGGTGGCCGTGGCTGGACTGCTCACTGTGCTT
NM_002808.4       TACCACAGCGACCGGCAGCTTATGAGCCAGGTGGCCGTGGCTGGACTGCTCACTGTGCTT
                  ************************************************************

HsCD00298487      GTCTCTTTCCTGGATGTTCGAAACATTATTCTAGGCAAATCACACTATGTATTGTATGGG
NM_002808.4       GTCTCTTTCCTGGATGTTCGAAACATTATTCTAGGCAAATCACACTATGTATTGTATGGG
                  ************************************************************

HsCD00298487      CTGGTGGCTGCCATGCAGCCCCGAATGCTGGTTACGTTTGATGAGGAGCTGCGGCCATTG
NM_002808.4       CTGGTGGCTGCCATGCAGCCCCGAATGCTGGTTACGTTTGATGAGGAGCTGCGGCCATTG
                  ************************************************************

HsCD00298487      CCAGTGTCTGTCCGTGTGGGCCAGGCAGTGGATGTGGTGGGCCAGGCTGGCAAGCCGAAG
NM_002808.4       CCAGTGTCTGTCCGTGTGGGCCAGGCAGTGGATGTGGTGGGCCAGGCTGGCAAGCCGAAG
                  ************************************************************

HsCD00298487      ACTATCACAGGGTTCCAGACGCATACAACCCCAGTGTTGTTGGCCCACGGGGAACGGGCA
NM_002808.4       ACTATCACAGGGTTCCAGACGCATACAACCCCAGTGTTGTTGGCCCACGGGGAACGGGCA
                  ************************************************************

HsCD00298487      GAATTGGCCACTGAGGAGTTTCTTCCTGTTACCCCCATTCTGGAAGGTTTTGTTATCCTT
NM_002808.4       GAATTGGCCACTGAGGAGTTTCTTCCTGTTACCCCCATTCTGGAAGGTTTTGTTATCCTT
                  ************************************************************

HsCD00298487      CGGAAGAACCCCAATTATGATCTCTTG
NM_002808.4       CGGAAGAACCCCAATTATGATCTCTAA
                  *************************:.

Pathways

Panther :

Ubiquitin proteasome pathway

Reactome :

Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins

Reactome :

Activation of NF-kappaB in B cells

Reactome :

Adaptive Immune System

Reactome :

Antigen processing-Cross presentation

Reactome :

Antigen processing: Ubiquitination & Proteasome degradation

Reactome :

APC/C-mediated degradation of cell cycle proteins

Reactome :

APC/C:Cdc20 mediated degradation of mitotic proteins

Reactome :

APC/C:Cdc20 mediated degradation of Securin

Reactome :

APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1

Reactome :

Apoptosis

Reactome :

Assembly of the pre-replicative complex

Reactome :

Asymmetric localization of PCP proteins

Reactome :

AUF1 (hnRNP D0) destabilizes mRNA

Reactome :

Autodegradation of Cdh1 by Cdh1:APC/C

Reactome :

Autodegradation of the E3 ubiquitin ligase COP1

Reactome :

beta-catenin independent WNT signaling

Reactome :

Cdc20:Phospho-APC/C mediated degradation of Cyclin A

Reactome :

CDK-mediated phosphorylation and removal of Cdc6

Reactome :

CDT1 association with the CDC6:ORC:origin complex

Reactome :

Cell Cycle

Reactome :

Cell Cycle Checkpoints

Reactome :

Cell Cycle, Mitotic

Reactome :

Class I MHC mediated antigen processing & presentation

Reactome :

Cross-presentation of soluble exogenous antigens (endosomes)

Reactome :

Cyclin A:Cdk2-associated events at S phase entry

Reactome :

Cyclin E associated events during G1/S transition

Reactome :

degradation of AXIN

Reactome :

degradation of DVL

Reactome :

Disease

Reactome :

DNA Replication

Reactome :

DNA Replication Pre-Initiation

Reactome :

Downstream signaling events of B Cell Receptor (BCR)

Reactome :

ER-Phagosome pathway

Reactome :

G1/S DNA Damage Checkpoints

Reactome :

G1/S Transition

Reactome :

Gene Expression

Reactome :

HIV Infection

Reactome :

Host Interactions of HIV factors

Reactome :

Immune System

Reactome :

M Phase

Reactome :

M/G1 Transition

Reactome :

Metabolism

Reactome :

Metabolism of amino acids and derivatives

Reactome :

Metabolism of mRNA

Reactome :

Metabolism of RNA

Reactome :

Mitotic Anaphase

Reactome :

Mitotic G1-G1/S phases

Reactome :

Mitotic M-M/G1 phases

Reactome :

Mitotic Metaphase and Anaphase

Reactome :

Orc1 removal from chromatin

Reactome :

p53-Dependent G1 DNA Damage Response

Reactome :

p53-Dependent G1/S DNA damage checkpoint

Reactome :

p53-Independent DNA Damage Response

Reactome :

p53-Independent G1/S DNA damage checkpoint

Reactome :

PCP/CE pathway

Reactome :

Regulation of activated PAK-2p34 by proteasome mediated degradation

Reactome :

Regulation of APC/C activators between G1/S and early anaphase

Reactome :

Regulation of Apoptosis

Reactome :

Regulation of DNA replication

Reactome :

Regulation of mitotic cell cycle

Reactome :

Regulation of mRNA stability by proteins that bind AU-rich elements

Reactome :

Regulation of ornithine decarboxylase (ODC)

Reactome :

Removal of licensing factors from origins

Reactome :

S Phase

Reactome :

SCF(Skp2)-mediated degradation of p27/p21

Reactome :

SCF-beta-TrCP mediated degradation of Emi1

Reactome :

Separation of Sister Chromatids

Reactome :

Signal Transduction

Reactome :

Signaling by the B Cell Receptor (BCR)

Reactome :

Signaling by Wnt

Reactome :

Stabilization of p53

Reactome :

Switching of origins to a post-replicative state

Reactome :

Synthesis of DNA

Reactome :

TCF dependent signaling in response to WNT

Reactome :

Ubiquitin Mediated Degradation of Phosphorylated Cdc25A

Reactome :

Ubiquitin-dependent degradation of Cyclin D

Reactome :

Ubiquitin-dependent degradation of Cyclin D1

Reactome :

Vif-mediated degradation of APOBEC3G

Reactome :

Vpu mediated degradation of CD4

WikiPathway :

Proteasome Degradation

WikiPathway :

TNF alpha Signaling Pathway

Protein

UniProt :

Q13200

HPRD :

05870

PDBe Mol* Structure Viewer

Clone

Cloning Information :

230518

HIP Master Clone ID :

53223

Original Clone ID :

FLH230518.01L

Organism

Species Specific ID:

5708

Genome

Chromosome :

Map Location :

3q27.1

Ensembl :

ENSG00000175166

Reagents

Labome :

PSMD2-antibody

PSMD2 (H. sapiens )In pLDNT7_nFLAG (Flag-taggedIn vitro expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

PDBe Mol* Structure Viewer

Clone

Organism

Genome

Reagents

PSMD2 (H. sapiens )
In pLDNT7_nFLAG (Flag-tagged
In vitro expression vector)