Gene:
Gene Symbol:
CASK
Gene Name:
calcium/calmodulin-dependent serine protein kinase (MAGUK family)
Original Clone ID:
FLH230206.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
FUSION
Source:
HIP
Description:
None
Comments:
This clone is identical to HsCD00617921.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HsCD00298876
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCCGACGACGACGTGCTGTTCGAGGATGTGTACGAGCTGTGCGAGGTGATCGGAAAG
GGTCCCTTCAGTGTTGTACGACGATGTATCAACAGAGAAACTGGGCAACAATTTGCTGTA
AAAATTGTTGATGTAGCCAAGTTCACATCAAGTCCAGGGTTAAGTACAGAAGATCTAAAG
CGGGAAGCCAGTATCTGTCATATGCTGAAACATCCACACATTGTAGAGTTATTGGAGACA
TATAGCTCAGATGGAATGCTTTACATGGTTTTCGAATTTATGGATGGAGCAGATCTGTGT
TTTGAAATCGTAAAGCGAGCTGACGCTGGTTTTGTGTACAGTGAAGCTGTAGCCAGCCAT
TATATGAGACAGATACTGGAAGCTCTACGCTACTGCCATGATAATAACATAATTCACAGG
GATGTGAAGCCCCACTGTGTTCTCCTTGCCTCAAAAGAAAACTCGGCACCTGTTAAACTT
GGAGGCTTTGGGGTAGCTATTCAATTAGGGGAGTCTGGACTTGTAGCTGGAGGACGTGTT
GGAACACCTCATTTTATGGCACCAGAAGTGGTCAAAAGAGAGCCTTACGGAAAGCCTGTA
GACGTCTGGGGGTGCGGTGTGATCCTTTTTATCCTGCTCAGTGGTTGTTTGCCTTTTTAC
GGAACCAAGGAAAGATTGTTTGAAGGCATTATTAAAGGAAAATATAAGATGAATCCAAGG
CAGTGGAGCCATATCTCTGAAAGTGCCAAAGACCTAGTACGTCGCATGCTGATGCTGGAT
CCAGCTGAAAGGATCACTGTTTATGAAGCACTGAATCACCCATGGCTTAAGGAGCGGGAT
CGTTACGCCTACAAGATTCATCTTCCAGAAACAGTAGAGCAGCTGAGGAAATTCAATGCA
AGGAGGAAACTAAAGGGTGCAGTACTAGCCGCTGTGTCAAGTCACAAATTCAACTCATTC
TATGGGGATCCCCCTGAAGAGTTACCAGATTTCTCCGAAGACCCTACCTCCTCAGGAGCA
GTCTCACAGGTGCTGGACAGCCTGGAAGAGATTCATGCGCTTACAGACTGCAGTGAAAAG
GACCTAGATTTTCTACACAGTGTTTTCCAGGATCAGCATCTTCACACACTACTAGATCTG
TATGACAAAATTAACACAAAGTCTTCACCACAAATCAGGAATCCTCCAAGCGATGCAGTA
CAGAGAGCCAAAGAGGTATTGGAAGAAATTTCATGTTACCCTGAGAATAACGACGCAAAG
GAACTAAAGCGTATTTTAACACAACCTCATTTCATGGCCTTACTTCAGACTCACGACGTA
GTGGCACATGAAGTTTACAGTGATGAAGCATTGAGGGTCACACCTCCTCCCACCTCTCCC
TATTTAAACGGCGATTCTCCAGAAAGTGCTAACGGAGACATGGATATGGAGAATGTGACC
AGAGTTCGGCTGGTACAGTTTCAAAAGAACACAGATGAACCAATGGGAATCACTTTAAAA
ATGAATGAACTAAATCATTGTATTGTTGCAAGAATTATGCATGGGGGCATGATTCACAGG
CAAGGTACACTTCATGTTGGTGATGAAATTCGAGAAATCAATGGCATCAGTGTGGCTAAC
CAAACAGTGGAACAACTGCAAAAAATGCTTAGGGAAATGCGGGGGAGTATTACCTTCAAG
ATTGTGCCAAGTTACCGCACTCAGTCTTCGTCCTGTGAGGACTTGCCATCAACTACCCAA
CCAAAAGGACGACAGATCTATGTAAGAGCACAATTTGAATATGATCCAGCCAAGGATGAC
CTCATCCCCTGTAAAGAAGCTGGCATTCGATTCAGAGTTGGTGACATCATCCAGATTATT
AGTAAGGATGATCATAATTGGTGGCAGGGTAAACTGGAAAACTCCAAAAATGGAACTGCA
GGTCTCATTCCTTCTCCTGAACTTCAGGAATGGCGAGTAGCTTGCATTGCCATGGAGAAG
ACCAAACAGGAGCAGCAGGCCAGCTGTACTTGGTTTGGCAAGAAAAAGAAGCAGTACAAA
GATAAATATTTGGCAAAGCACAATGCAGTGTTTGATCAATTAGATCTTGTCACATATGAA
GAAGTAGTAAAACTGCCAGCATTCAAGAGGAAAACACTAGTCTTATTAGGCGCACATGGT
GTTGGGAGAAGACACATAAAAAACACTCTCATCACAAAGCACCCAGACCGGTTTGCGTAC
CCTATTCCACATACAACCAGACCTCCAAAGAAAGACGAAGAAAATGGAAAGAATTATTAC
TTTGTATCTCATGACCAAATGATGCAAGACATCTCTAATAACGAGTACTTGGAGTACGGC
AGCCACGAGGATGCGATGTATGGGACAAAACTGGAGACCATCCGGAAGATCCACGAGCAG
GGGCTGATTGCAATACTGGACGTGGAGCCTCAGGCACTGAAGGTCCTGAGAACTGCAGAG
TTTGCTCCTTTTGTTGTTTTCATTGCTGCACCAACTATTACTCCAGGTTTAAATGAGGAT
GAATCTCTTCAGCGTCTGCAGAAGGAGTCTGACATCTTACAGAGAACATATGCACACTAC
TTCGATCTCACAATTATCAACAATGAAATTGATGAGACAATCAGACATCTGGAGGAAGCT
GTTGAGCTCGTGTGCACAGCCCCACAGTGGGTCCTTGTCTCCTGGGTCTATTTG
Protein sequence predicted based on nucleotide sequence
MADDDVLFEDVYELCEVIGKGPFSVVRRCINRETGQQFAVKIVDVAKFTSSPGLSTEDLK REASICHMLKHPHIVELLETYSSDGMLYMVFEFMDGADLCFEIVKRADAGFVYSEAVASH YMRQILEALRYCHDNNIIHRDVKPHCVLLASKENSAPVKLGGFGVAIQLGESGLVAGGRV GTPHFMAPEVVKREPYGKPVDVWGCGVILFILLSGCLPFYGTKERLFEGIIKGKYKMNPR QWSHISESAKDLVRRMLMLDPAERITVYEALNHPWLKERDRYAYKIHLPETVEQLRKFNA RRKLKGAVLAAVSSHKFNSFYGDPPEELPDFSEDPTSSGAVSQVLDSLEEIHALTDCSEK DLDFLHSVFQDQHLHTLLDLYDKINTKSSPQIRNPPSDAVQRAKEVLEEISCYPENNDAK ELKRILTQPHFMALLQTHDVVAHEVYSDEALRVTPPPTSPYLNGDSPESANGDMDMENVT RVRLVQFQKNTDEPMGITLKMNELNHCIVARIMHGGMIHRQGTLHVGDEIREINGISVAN QTVEQLQKMLREMRGSITFKIVPSYRTQSSSCEDLPSTTQPKGRQIYVRAQFEYDPAKDD LIPCKEAGIRFRVGDIIQIISKDDHNWWQGKLENSKNGTAGLIPSPELQEWRVACIAMEK TKQEQQASCTWFGKKKKQYKDKYLAKHNAVFDQLDLVTYEEVVKLPAFKRKTLVLLGAHG VGRRHIKNTLITKHPDRFAYPIPHTTRPPKKDEENGKNYYFVSHDQMMQDISNNEYLEYG SHEDAMYGTKLETIRKIHEQGLIAILDVEPQALKVLRTAEFAPFVVFIAAPTITPGLNED ESLQRLQKESDILQRTYAHYFDLTIINNEIDETIRHLEEAVELVCTAPQWVLVSWVYL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence
2793
Start on reference sequence
28
Mutations:
None
Discrepancy:
del@1016,18;g1436a,G479D;
del@1737,69;t2023c,S675P;
ins@2153,15;g2324a,R775K;
c2747t,P916L;
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
