Gene:
Gene Symbol:
RBL2
Gene Name:
retinoblastoma-like 2 (p130)
Original Clone ID:
FLH232446.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
FUSION
Source:
HIP
Description:
None
Comments:
This clone is identical to HsCD00616865
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HsCD00298945
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCGTCGGGAGGTGACCAGTCGCCACCGCCCCCGCCTCCCCCTCCGGCGGCGGCAGCC
TCGGATGAGGAGGAGGAGGACGACGGCGAGGCGGAAGACGCCGCGCCGCCTGCCGAGTCG
CCCACCCCTCAGATCCAGCAGCGGTTCGACGAGCTGTGCAGCCGCCTCAACATGGACGAG
GCGGCGCGGGCCGAGGCCTGGGACAGCTACCGCAGCATGAGCGAAAGCTACACGCTGGAG
GGAAATGATCTTCATTGGTTAGCATGTGCCTTATATGTGGCTTGCAGAAAATCTGTTCCA
ACTGTAAGCAAAGGGACAGTGGAAGGAAACTATGTATCTTTAACTAGAATCCTGAAATGT
TCAGAGCAGAGCTTAATCGAATTTTTTAATAAGATGAAGAAGTGGGAAGACATGGCAAAT
CTACCCCCACATTTCAGAGAACGTACTGAGAGATTAGAAAGAAACTTCACTGTTTCTGCT
GTAATTTTTAAGAAATATGAACCCATTTTTCAGGACATCTTTAAATACCCTCAAGAGGAG
CAACCTCGTCAGCAGCGAGGAAGGAAACAGCGGCGACAGCCCTGTACTGTGTCTGAAATT
TTCCATTTTTGTTGGGTGCTTTTTATATATGCAAAAGGTAATTTCCCCATGATTAGTGAT
GATTTGGTCAATTCTTATCACCTGCTGCTGTGTGCTTTGGACTTAGTTTATGGAAATGCA
CTTCAGTGTTCTAATCGTAAAGAACTTGTGAACCCTAATTTTAAAGGCTTATCTGAAGAT
TTTCATGCTAAAGATTCTAAACCTTCCTCTGACCCCCCTTGTATCATTGAGAAACTGTGT
TCCTTACATGATGGCCTAGTTTTGGAAGCAAAGGGGATAAAGGAACATTTCTGGAAACCC
TATATTAGGAAACTTTATGAAAAAAAGCTCCTTAAGGGAAAAGAAGAAAATCTCACTGGG
TTTCTAGAACCTGGGAACTTTGGAGAGAGTTTTAAAGCCATCAATAAGGCCTATGAGGAG
TATGTTTTATCTGTTGGGAATTTAGATGAGCGGATATTTCTTGGAGAGGATGCTGAGGAG
GAAATTGGGACTCTCTCAAGGTGTCTGAACGCTGGTTCAGGAACAGAGACTGCTGAAAGG
GTGCAGATGAAAAACATCTTACAGCAGCATTTTGACAAGTCCAAAGCACTTAGAATCTCC
ACACCACTAACTGGTGTTAGGTACATTAAGGAGAATAGCCCTTGTGTGACTCCAGTTTCT
ACAGCTACGCATAGCTTGAGTCGTCTTCACACCATGCTGACAGGCCTCAGGAATGCACCA
AGTGAGAAACTGGAACAGATTCTCAGGACATGTTCCAGAGATCCAACCCAGGCTATTGCT
AACAGACTGAAAGAAATGTTTGAAATATATTCTCAGCATTTCCAGCCAGACGAGGATTTC
AGTAATTGTGCTAAAGAAATTGCCAGCAAACATTTTCGTTTTGCGGAGATGCTTTACTAT
AAAGTATTAGAATCTGTTATTGAGCAGGAACAAAAAAGACTAGGAGACATGGATTTATCT
GGTATTCTGGAACAAGATGCGTTCCACAGATCTCTCTTGGCCTGCTGCCTTGAGGTCGTC
ACTTTTTCTTATAAGCCTCCTGGGAATTTTCCATTTATTACTGAAATATTTGATGTGCCT
CTTTATCATTTTTATAAGGTGATAGAAGTATTCATTAGAGCAGAAGATGGCCTTTGTAGA
GAGGTGGTAAAACACCTTAATCAGATTGAAGAACAGATCTTAGATCATTTGGCATGGAAA
CCAGAGTCTCCACTCTGGGAAAAAATTAGAGACAATGAAAACAGAGTTCCTACATGTGAA
GAGGTCATGCCACCTCAGAACCTGGAAAGGGCAGATGAAATTTGCATTGCTGGCTCCCCT
TTGACTCCCAGAAGGGTGACTGAAGTTCGTGCTGATACTGGAGGACTTGGAAGGAGCATA
ACATCTCCAACCACATTATACGATAGGTACAGCTCCCCACCAGCCAGCACTACCAGAAGG
CGGCTATTTGTTGAGAATGATAGCCCCTCTGATGGAGGGACGCCTGGGCGCATGCCCCCA
CAGCCCCTAGTCAATGCTGTCCCTGTGCAGAATGTATCTGGGGAGACTGTTTCTGTCACA
CCAGTTCCTGGACAGACTTTGGTCACCATGGCAACCGCCACTGTCACAGCCAACAATGGG
CAAACGGTAACCATTCCTGTGCAAGGTATTGCCAATGAAAATGGAGGGATAACATTCTTC
CCTGTCCAAGTCAATGTTGGGGGGCAGGCACAAGCTGTGACAGGCTCCATCCAGCCCCTC
AGTGCTCAGGCCCTGGCTGGAAGTCTGAGCTCTCAACAGGTGACAGGAACAACTTTGCAA
GTCCCTGGTCAAGTGGCCATTCAACAGATTTCCCCAGGTGGCCAACAGCAGAAGCAAGGC
CAGTCTGTAACCAGCAGTAGTAATAGACCCAGGAAGACCAGCTCTTTATCGCTTTTCTTT
AGAAAGGTATACCATTTAGCAGCTGTCCGCCTTCGGGATCTCTGTGCCAAACTAGATATT
TCAGATGAATTGAGGAAAAAAATCTGGACCTGCTTTGAATTCTCCATAATTCAGTGTCCT
GAACTTATGATGGACAGACATCTGGACCAGTTATTAATGTGTGCCATTTATGTGATGGCA
AAGGTCACAAAAGAAGATAAGTCCTTCCAGAACATTATGCGTTGTTATAGGACTCAGCCG
CAGGCCCGGAGCCAGGTGTATAGAAGTGTTTTGATAAAAGGAAAAAGAAAAAGAAGAAAT
TCTGGCAGCAGTGATAGCAGAAGCCATCAGAATTCTCCAACAGAACTAAACAAAGATAGA
ACCAGTAGAGACTCCAGTCCAGTTATGAGGTCAAGCAGCACCTTGCCAGTTCCACAGCCC
AGCAGTGCTCCTCCCACACCTACTCGCCTCACAGGTGCCAACAGTGACATGGAAGAAGAG
GAGAGGGGAGACCTCATTCAGTTCTACAACAACATCTACATCAAACAGATTAAGACATTT
GCCATGAAGTACTCACAGGCAAATATGGATGCTCCTCCACTCTCTCCCTATCCATTTGTA
AGAACAGGCTCCCCTCGCCGAATACAGTTGTCTCAAAATCATCCTGTCTACATTTCCCCA
CATAAAAATGAAACAATGCTTTCTCCTCGAGAAAAGATTTTCTATTACTTCAGCAACAGT
CCTTCAAAGAGACTGAGAGAAATTAATAGTATGATACACACAGGAGAAACTCCTACTAAA
AAGAGAGGAATTCTTTTGGAAGATGGAAGTGAATCACCTGCAAAAAGAATTTGCCCAGAA
AATCATTCTGCCTTATTACGCCGTCTCCAAGATGTAGCTAATGACCGTGGTTCCCACTTG
Protein sequence predicted based on nucleotide sequence
MPSGGDQSPPPPPPPPAAAASDEEEEDDGEAEDAAPPAESPTPQIQQRFDELCSRLNMDE AARAEAWDSYRSMSESYTLEGNDLHWLACALYVACRKSVPTVSKGTVEGNYVSLTRILKC SEQSLIEFFNKMKKWEDMANLPPHFRERTERLERNFTVSAVIFKKYEPIFQDIFKYPQEE QPRQQRGRKQRRQPCTVSEIFHFCWVLFIYAKGNFPMISDDLVNSYHLLLCALDLVYGNA LQCSNRKELVNPNFKGLSEDFHAKDSKPSSDPPCIIEKLCSLHDGLVLEAKGIKEHFWKP YIRKLYEKKLLKGKEENLTGFLEPGNFGESFKAINKAYEEYVLSVGNLDERIFLGEDAEE EIGTLSRCLNAGSGTETAERVQMKNILQQHFDKSKALRISTPLTGVRYIKENSPCVTPVS TATHSLSRLHTMLTGLRNAPSEKLEQILRTCSRDPTQAIANRLKEMFEIYSQHFQPDEDF SNCAKEIASKHFRFAEMLYYKVLESVIEQEQKRLGDMDLSGILEQDAFHRSLLACCLEVV TFSYKPPGNFPFITEIFDVPLYHFYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWK PESPLWEKIRDNENRVPTCEEVMPPQNLERADEICIAGSPLTPRRVTEVRADTGGLGRSI TSPTTLYDRYSSPPASTTRRRLFVENDSPSDGGTPGRMPPQPLVNAVPVQNVSGETVSVT PVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQVNVGGQAQAVTGSIQPL SAQALAGSLSSQQVTGTTLQVPGQVAIQQISPGGQQQKQGQSVTSSSNRPRKTSSLSLFF RKVYHLAAVRLRDLCAKLDISDELRKKIWTCFEFSIIQCPELMMDRHLDQLLMCAIYVMA KVTKEDKSFQNIMRCYRTQPQARSQVYRSVLIKGKRKRRNSGSSDSRSHQNSPTELNKDR TSRDSSPVMRSSSTLPVPQPSSAPPTPTRLTGANSDMEEEERGDLIQFYNNIYIKQIKTF AMKYSQANMDAPPLSPYPFVRTGSPRRIQLSQNHPVYISPHKNETMLSPREKIFYYFSNS PSKRLREINSMIHTGETPTKKRGILLEDGSESPAKRICPENHSALLRRLQDVANDRGSHL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
109
End on reference sequence
3528
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
