Gene:
Gene Symbol:
ITGB5
Gene Name:
integrin, beta 5
Original Clone ID:
FLH271473.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
FUSION
Source:
HIP
Description:
None
Comments:
This clone is identical to HsCD00617819
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HsCD00300264
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCGCGGGCCCCGGCGCCGCTGTACGCCTGCCTCCTGGGGCTCTGCGCGCTCCTGCCC
CGGCTCGCAGGTCTCAACATATGCACTAGTGGAAGTGCCACCTCATGTGAAGAATGTCTG
CTAATCCACCCAAAATGTGCCTGGTGCTCCAAAGAGGACTTCGGAAGCCCACGGTCCATC
ACCTCTCGGTGTGATCTGAGGGCAAACCTTGTCAAAAATGGCTGTGGAGGTGAGATAGAG
AGCCCAGCCAGCAGCTTCCATGTCCTGAGGAGCCTGCCCCTCAGCAGCAAGGGTTCGGGC
TCTGCAGGCTGGGACGTCATTCAGATGACACCACAGGAGATTGCCGTGAACCTCCGGCCC
GGTGACAAGACCACCTTCCAGCTACAGGTTCGCCAGGTGGAGGACTATCCTGTGGACCTG
TACTACCTGATGGACCTCTCCCTGTCCATGAAGGATGACTTGGACAATATCCGGAGCCTG
GGCACCAAACTCGCGGAGGAGATGAGGAAGCTCACCAGCAACTTCCGGTTGGGATTTGGG
TCTTTTGTTGATAAGGACATCTCTCCTTTCTCCTACACGGCACCGAGGTACCAGACCAAT
CCGTGCATTGGTTACAAGTTGTTTCCAAATTGCGTCCCCTCCTTTGGGTTCCGCCATCTG
CTGCCTCTCACAGACAGAGTGGACAGCTTCAATGAGGAAGTTCGGAAACAGAGGGTGTCC
CGGAACCGAGATGCCCCTGAGGGGGGCTTTGATGCAGTACTCCAGGCAGCCGTCTGCAAG
GAGAAGATTGGCTGGCGAAAGGATGCACTGCATTTGCTGGTGTTCACAACAGATGATGTG
CCCCACATCGCATTGGATGGAAAATTGGGAGGCCTGGTGCAGCCACACGATGGCCAGTGC
CACCTGAACGAGGCCAACGAGTACACTGCATCCAACCAGATGGACTATCCATCCCTTGCC
TTGCTTGGAGAGAAATTGGCAGAGAACAACATCAACCTCATCTTTGCAGTGACAAAAAAC
CATTATATGCTGTACAAGAATTTTACAGCCCTGATACCTGGAACAACGGTGGAGATTTTA
GATGGAGACTCCAAAAATATTATTCAACTGATTATTAATGCATACAATAGTATCCGGTCT
AAAGTGGAGTTGTCAGTCTGGGATCAGCCTGAGGATCTTAATCTCTTCTTTACTGCTACC
TGCCAAGATGGGGTATCCTATCCTGGTCAGAGGAAGTGTGAGGGTCTGAAGATTGGGGAC
ACGGCATCTTTTGAAGTATCATTGGAGGCCCGAAGCTGTCCCAGCAGACACACGGAGCAT
GTGTTTGCCCTGCGGCCGGTGGGATTCCGGGACAGCCTGGAGGTGGGGGTCACCTACAAC
TGCACGTGCGGCTGCAGCGTGGGGCTGGAACCCAACAGTGCCAGGTGCAACGGGAGCGGG
ACCTATGTCTGCGGCCTGTGTGAGTGCAGCCCCGGCTACCTGGGCACCAGGTGCGAGTGC
CAGGATGGGGAGAACCAGAGCGTGTACCAGAACCTGTGCCGGGAGGCAGAGGGCAAGCCA
CTGTGCAGCGGGCGTGGGGACTGCAGCTGCAACCAGTGCTCCTGCTTCGAGAGCGAGTTC
GGCAAGATCTATGGGCCTTTCTGTGAGTGCGACAACTTCTCCTGTGCCAGGAACAAGGGA
GTCCTCTGCTCAGGCCATGGCGAGTGTCACTGCGGGGAATGCAAGTGCCATGCAGGTTAC
ATCGGGGACAACTGTAACTGCTCGACAGACATCAGCACATGCCGGGGCAGAGATGGCCAG
ATCTGCAGCGAGCGTGGGCACTGTCTCTGTGGGCAGTGCCAATGCACGGAGCCGGGGGCC
TTTGGGGAGATGTGTGAGAAGTGCCCCACCTGCCCGGATGCATGCAGCACCAAGAGAGAT
TGCGTCGAGTGCCTGCTGCTCCACTCTGGGAAACCTGACAACCAGACCTGCCACAGCCTA
TGCAGGGATGAGGTGATCACATGGGTGGACACCATCGTGAAAGATGACCAGGAGGCTGTG
CTATGTTTCTACAAAACCGCCAAGGACTGCGTCATGATGTTCACCTATGTGGAGCTCCCC
AGTGGGAAGTCCAACCTGACCGTCCTCAGGGAGCCAGAGTGTGGAAACACCCCCAACGCC
ATGACCATCCTCCTGGCTGTGGTCGGTAGCATCCTCCTTGTTGGGCTTGCACTCCTGGCT
ATCTGGAAGCTGCTTGTCACCATCCACGACCGGAGGGAGTTTGCAAAGTTTCAGAGCGAG
CGATCCAGGGCCCGCTATGAAATGGCTTCAAATCCATTATACAGAAAGCCTATCTCCACG
CACACTGTGGACTTCACCTTCAACAAGTTCAACAAATCCTACAATGGCACTGTGGACTTG
Protein sequence predicted based on nucleotide sequence
MPRAPAPLYACLLGLCALLPRLAGLNICTSGSATSCEECLLIHPKCAWCSKEDFGSPRSI TSRCDLRANLVKNGCGGEIESPASSFHVLRSLPLSSKGSGSAGWDVIQMTPQEIAVNLRP GDKTTFQLQVRQVEDYPVDLYYLMDLSLSMKDDLDNIRSLGTKLAEEMRKLTSNFRLGFG SFVDKDISPFSYTAPRYQTNPCIGYKLFPNCVPSFGFRHLLPLTDRVDSFNEEVRKQRVS RNRDAPEGGFDAVLQAAVCKEKIGWRKDALHLLVFTTDDVPHIALDGKLGGLVQPHDGQC HLNEANEYTASNQMDYPSLALLGEKLAENNINLIFAVTKNHYMLYKNFTALIPGTTVEIL DGDSKNIIQLIINAYNSIRSKVELSVWDQPEDLNLFFTATCQDGVSYPGQRKCEGLKIGD TASFEVSLEARSCPSRHTEHVFALRPVGFRDSLEVGVTYNCTCGCSVGLEPNSARCNGSG TYVCGLCECSPGYLGTRCECQDGENQSVYQNLCREAEGKPLCSGRGDCSCNQCSCFESEF GKIYGPFCECDNFSCARNKGVLCSGHGECHCGECKCHAGYIGDNCNCSTDISTCRGRDGQ ICSERGHCLCGQCQCTEPGAFGEMCEKCPTCPDACSTKRDCVECLLLHSGKPDNQTCHSL CRDEVITWVDTIVKDDQEAVLCFYKTAKDCVMMFTYVELPSGKSNLTVLREPECGNTPNA MTILLAVVGSILLVGLALLAIWKLLVTIHDRREFAKFQSERSRARYEMASNPLYRKPIST HTVDFTFNKFNKSYNGTVDL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence
2706
Start on reference sequence
307
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
