Gene:
Gene Symbol:
KCND1
Gene Name:
potassium voltage-gated channel, Shal-related subfamily, member 1
Original Clone ID:
FLH271348.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
This clone is identical to HsCD00617888
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HsCD00300497
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGGCAGGCCTGGCCACGTGGCTGCCTTTTGCTCGGGCAGCAGCAGTGGGCTGGCTG
CCCCTGGCCCAGCAACCCCTGCCCCCGGCACCGGGGGTGAAGGCATCTCGAGGAGATGAG
GTTCTGGTGGTGAACGTGAGCGGACGGCGCTTTGAGACTTGGAAGAATACGCTGGACCGC
TACCCAGACACCTTGCTGGGCAGCTCGGAGAAGGAATTCTTCTACGATGCTGACTCAGGC
GAGTACTTCTTCGATCGCGACCCTGACATGTTCCGCCATGTGCTGAACTTCTACCGAACG
GGGCGGCTGCATTGCCCACGGCAGGAGTGCATCCAGGCCTTCGACGAAGAGCTGGCTTTC
TACGGCCTGGTTCCCGAGCTAGTCGGTGACTGCTGCCTTGAAGAGTATCGGGACCGAAAG
AAGGAGAATGCCGAGCGCCTGGCAGAGGATGAGGAGGCAGAGCAGGCCGGGGACGGCCCA
GCCCTGCCAGCAGGCAGCTCCCTGCGGCAGCGGCTCTGGCGGGCCTTCGAGAATCCACAC
ACGAGCACCGCAGCCCTCGTTTTCTACTATGTGACCGGCTTCTTCATCGCCGTGTCGGTC
ATCGCCAATGTGGTGGAGACCATCCCATGCCGCGGCTCTGCACGCAGGTCCTCAAGGGAG
CAGCCCTGTGGCGAACGCTTCCCACAGGCCTTTTTCTGCATGGACACAGCCTGTGTACTC
ATATTCACAGGTGAATACCTCCTGCGGCTGTTTGCCGCCCCCAGCCGTTGCCGCTTCCTG
CGGAGTGTCATGAGCCTCATCGACGTGGTGGCCATCCTGCCCTACTACATTGGGCTTTTG
GTGCCCAAGAACGACGATGTCTCTGGCGCCTTTGTCACCCTGCGTGTGTTCCGGGTGTTT
CGCATCTTCAAGTTCTCCAGGCACTCACAGGGCTTGAGGATTCTGGGCTACACACTCAAG
AGCTGTGCCTCTGAGCTGGGCTTTCTCCTCTTTTCCCTAACCATGGCCATCATCATCTTT
GCCACTGTCATGTTTTATGCTGAGAAGGGCACAAACAAGACCAACTTTACAAGCATCCCT
GCGGCCTTCTGGTATACCATTGTCACCATGACCACGCTTGGCTACGGAGACATGGTGCCC
AGCACCATTGCTGGCAAGATTTTCGGGTCCATCTGCTCACTCAGTGGCGTCTTGGTCATT
GCCCTGCCTGTGCCAGTCATTGTGTCCAACTTTAGCCGCATCTACCACCAGAACCAGCGG
GCTGACAAGCGCCGAGCACAGCAGAAGGTGCGCTTGGCAAGGATCCGATTGGCAAAGAGT
GGTACCACCAATGCCTTCCTGCAGTACAAGCAGAATGGGGGCCTTGAGGACAGCGGCAGT
GGCGAGGAACAGGCTCTTTGTGTCAGGAACCGTTCTGCCTTTGAACAGCAACATCACCAC
TTGCTGCACTGTCTAGAGAAGACAACGTGCCATGAGTTCACAGATGAGCTCACCTTCAGT
GAAGCCCTGGGAGCCGTCTCGCCGGGTGGCCGCACCAGCCGTAGCACCTCTGTGTCTTCC
CAGCCAGTGGGACCCGGAAGCCTGCTGTCTTCTTGCTGCCCTCGCAGGGCCAAGCGCCGC
GCCATCCGCCTTGCCAACTCCACTGCCTCAGTCAGCCGTGGCAGCATGCAGGAGCTGGAC
ATGCTGGCAGGGCTGCGCAGGAGCCATGCCCCTCAGAGCCGCTCCAGCCTCAATGCCAAG
CCCCATGACAGCCTTGACCTGAACTGCGACAGCCGGGACTTCGTGGCTGCCATTATCAGC
ATCCCTACCCCTCCTGCCAACACCCCAGATGAGAGCCAACCTTCCTCCCCTGGCGGCGGT
GGCAGGGCCGGCAGCACCCTCAGGAACTCCAGCCTGGGTACCCCTTGCCTCTTCCCCGAG
ACTGTCAAGATCTCATCCCTGTAG
Protein sequence predicted based on nucleotide sequence
MAAGLATWLPFARAAAVGWLPLAQQPLPPAPGVKASRGDEVLVVNVSGRRFETWKNTLDR YPDTLLGSSEKEFFYDADSGEYFFDRDPDMFRHVLNFYRTGRLHCPRQECIQAFDEELAF YGLVPELVGDCCLEEYRDRKKENAERLAEDEEAEQAGDGPALPAGSSLRQRLWRAFENPH TSTAALVFYYVTGFFIAVSVIANVVETIPCRGSARRSSREQPCGERFPQAFFCMDTACVL IFTGEYLLRLFAAPSRCRFLRSVMSLIDVVAILPYYIGLLVPKNDDVSGAFVTLRVFRVF RIFKFSRHSQGLRILGYTLKSCASELGFLLFSLTMAIIIFATVMFYAEKGTNKTNFTSIP AAFWYTIVTMTTLGYGDMVPSTIAGKIFGSICSLSGVLVIALPVPVIVSNFSRIYHQNQR ADKRRAQQKVRLARIRLAKSGTTNAFLQYKQNGGLEDSGSGEEQALCVRNRSAFEQQHHH LLHCLEKTTCHEFTDELTFSEALGAVSPGGRTSRSTSVSSQPVGPGSLLSSCCPRRAKRR AIRLANSTASVSRGSMQELDMLAGLRRSHAPQSRSSLNAKPHDSLDLNCDSRDFVAAIIS IPTPPANTPDESQPSSPGGGGRAGSTLRNSSLGTPCLFPETVKISSL*
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1572
End on reference sequence
3515
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
