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Detailed Vector Information: pMIS2.1mv

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMIS2.1mv              
Source: Center for Structures of Membrane Proteins
Description: Gateway compatible bacterial expression destination vector with N-terminal 8xHis tag and Mistic (from B. subtilis) tag; contains ccdB death cassette that is removed after recombinational cloning; ampicillin resistance in bacteria; recombinational cloning.
Comments: None
Publications: PMID: 25155369
Title: Recombinant expression, purification and biophysical characterization of the transmembrane and membrane proximal domains of HIV-1 gp41.
Authors: Center for Structures of Membrane Proteins
Salk Institute
Mark Vega
PSI
EvNO00304415

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
       DyNA Vector Map

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Vector Name: pMIS2.1mv

pMIS2.1mv in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Gateway compatible bacterial expression destination vector with N-terminal 8xHis tag and Mistic (from B. subtilis) tag; contains ccdB death cassette that is removed after recombinational cloning; ampicillin resistance in bacteria; recombinational cloning.
Comments: Misticated vector with N-terminal HIS-tag based on pET vector with mistic from Bacilus subtilis.
Size (bp): 7562
Parent Vector: None
Properties: Gateway, acceptor (destination), bacterial expression, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: Center for Structures of Membrane Proteins
Salk Institute
Mark Vega
PSI
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS multiple cloning site 2289 2309
bacterial origin ColE ColE bacterial origin 4020 4702
death cassette ccdB ccdB death casette. Empty vector requires survival strain for growth 1883 2092
phage origin f1 f1 origin 2495 2801
protease cleavage site Thrombin thrombin cleavage site 145 162
recombination site attR1 attR1 recombinational cloning site 556 572
recombination site attR2 attR2 recombinational cloning site 2243 2258
repressor protein gene LacI Lac repressor gene 6114 7130
selectable marker AmpR Ampicillin resistance gene 3263 3922
tag MISTIC N-terminal Mistic protein (bacilus subtilis) tag. Used to increase membrane protein expression 247 498
tag His N-terminal 8xHis tag 2310 2327
trxn termination sequence lac operator lac operator 40 62
trxn termination sequence T7 T7 transcription terminator 2395 2441

 

Species Specific ID: None
Target GenBank: None