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Detailed Vector Information: pSGP46

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pSGP46              
Source: Center for High-Throughput Structural Biology
Description: Yeast expression vector with Adh2 promoter, adds C-terminal 3C-ZZ-10XHis tag; ampicillin resistance (bacteria); uracil resistance (yeast); ligation independent cloning (LIC).
Comments: None
Publications: PMID: 20045057
Title: Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography
Authors: PSI
Center for High Throughput Structural Biology
Mark Dumont
Kathleen Clark
EvNO00304416

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  yeast    Marker:  URA
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Vector Name: pSGP46

pSGP46 in advanced viewed

Synonyms: None
Description: Yeast expression vector with Adh2 promoter, adds C-terminal 3C-ZZ-10XHis tag; ampicillin resistance (bacteria); uracil resistance (yeast); ligation independent cloning (LIC).
Comments: S. cerevisiae expression strain
Size (bp): 6675
Parent Vector: None
Properties: ligation independent cloning (LIC), with tag/fusion/marker, yeast expression
Author Name: PSI
Center for High Throughput Structural Biology
Mark Dumont
Kathleen Clark
Publications: PMID: 20045057
Title: Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ligation independent cloning LIC Ligation independent cloning site 0 0
promoter Ura3 promoter Ura3 promoter 6360 6389
protease cleavage site 3C Rhinovirus 3C protease cleavage site 4233 4256
selectable marker AmpR Ampicillin resistance gene 1742 2401
selectable marker Ura3 Ura3 nutritional marker (yeast) 5471 6274
tag His 10x His tag 4653 4682

 

Species Specific ID: None
Target GenBank: None