DNASU Plasmid Repository • 480.965.5697 | Email

Detailed Vector Information: pSGP40

TOP

Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pSGP40              
Source: Center for High-Throughput Structural Biology
Description: Yeast expression vector with Gal1 promoter, adds C-terminal 3C-ZZ-His10 tag; amp resistance (URA for yeast); ligation independent cloning (LIC).
Comments: None
Publications: None
Authors: Center for High Throughput Structural Biology
Mark Dumont
Kathleen Clark
PSI
EvNO00304419

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  yeast    Marker:  URA
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pSGP40

pSGP40 in advanced viewed

Synonyms: pSGP-40
Sequencing Primer: Forward:  pSGP-Forward
Reverse:  pSGP-Reverse
Description: Yeast expression vector with Gal1 promoter, adds C-terminal 3C-ZZ-His10 tag; amp resistance (URA for yeast); ligation independent cloning (LIC).
Comments: None
Size (bp): 6564
Parent Vector: None
Properties: inducible expression, ligation independent cloning (LIC), with tag/fusion/marker, yeast expression, yeast shuttle
Author Name: Center for High Throughput Structural Biology
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 2499 3181
promoter T7 T7 promoter 4588 4608
promoter Ura3 promoter Ura3 promoter 6249 6278
protease cleavage site 3C 3C protease cleavage site 4122 4145
reporter gene LacZ LacZ alpha gene (beta-galactosidase) 4705 4773
selectable marker AmpR ampicillin resistance gene 1742 2401
selectable marker Ura3 URA3 nutritional marker 5360 6163
tag His 10xHis tag (LIC-3C-ZZ-His10) 4542 4571
trxn regulatory element lac operator lac operator 3521 3543

 

Species Specific ID: None
Target GenBank: None