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GPR23 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  LPAR4
Gene Name:  lysophosphatidic acid receptor 4
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH272097.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: BMS
Description: None
Comments: Clone insert was fully sequence-verified by the clone submitter but the sequence is not available.
Mutation/
Discrepancy :
Unknown / Unknown
Publications: None
Authors: Bristol-Myers Squibb
HsCD00305000

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1113nts         Open reading frame : 1 to 1113
Reference Sequence Alignment

ATGGGTGACAGAAGATTCATTGACTTCCAATTCCAAGATTCAAATTCAAGCCTCAGACCC
AGGTTGGGCAATGCTACTGCCAATAATACTTGCATTGTTGATGATTCCTTCAAGTATAAT
CTCAATGGTGCTGTCTACAGTGTTGTATTCATCTTGGGTCTGATAACCAACAGTGTCTCT
CTGTTTGTCTTCTGTTTCCGCATGAAAATGAGAAGTGAGACTGCTATTTTTATCACCAAT
CTAGCTGTCTCTGATTTGCTTTTTGTCTGTACACTACCTTTTAAAATATTTTACAACTTC
AACCGCCACTGGCCTTTTGGTGACACCCTCTGCAAGATCTCTGGAACTGCATTCCTTACC
AACATCTATGGGAGCATGCTCTTTCTCACCTGTATTAGTGTGGATCGTTTCCTGGCCATT
GTCTATCCTTTTCGATCTCGTACTATTAGGACTAGGAGGAATTCTGCCATTGTGTGTGCT
GGTGTCTGGATCCTAGTCCTCAGTGGCGGTATTTCAGCCTCTTTGTTTTCCACCACTAAT
GTCAACAATGCAACCACCACCTGCTTTGAAGGCTTCTCCAAACGTGTCTGGAAGACTTAT
TTATCCAAGATCACAATATTTATTGAAGTTGTTGGGTTTATCATTCCTCTAATATTGAAT
GTCTCTTGCTCTTCTGTGGTGCTGAGAACTCTTCGCAAGCCTGCTACTCTGTCTCAAATT
GGGACCAATAAGAAAAAAGTACTGAAAATGATCACAGTACATATGGCAGTCTTTGTGGTA
TGCTTTGTACCCTACAACTCTGTCCTCTTCTTGTATGCCCTGGTGCGCTCCCAAGCTATT
ACTAATTGCTTTTTGGAAAGATTTGCAAAGATCATGTACCCAATCACCTTGTGCCTTGCA
ACTCTGAACTGTTGTTTTGACCCTTTCATCTATTACTTCACCCTTGAATCCTTTCAGAAG
TCCTTCTACATCAATGCCCACATCAGAATGGAGTCCCTGTTTAAGACTGAAACACCTTTG
ACCACAAAGCCTTCCCTTCCAGCTATTCAAGAGGAAGTGAGTGATCAAACAACAAATAAT
GGTGGTGAATTAATGCTAGAATCCACCTTTTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1179
Start on reference sequence 67
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
(goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome

Gene

 

Gene Symbol : LPAR4
Symbol Nomenclature : LPAR4
SYNONYM : GPR23; LPA4; P2RY9; P2Y5-LIKE; P2Y9
Designation : G protein-coupled receptor 23|G-protein coupled receptor 23|LPA receptor 4|LPA-4|P2Y purinoceptor 9|P2Y5-like receptor|purinergic receptor 9
Full Nomenclature : lysophosphatidic acid receptor 4
GENEID : 2846
HGNC : 4478
MIM : 300086
Vega : OTTHUMG00000021895
Target GenBank: NM_005296

 

Reference Sequence Alignment

  

HsCD00305000        ATGGGTGACAGAAGATTCATTGACTTCCAATTCCAAGATTCAAATTCAAGCCTCAGACCC
NM_005296.2         ATGGGTGACAGAAGATTCATTGACTTCCAATTCCAAGATTCAAATTCAAGCCTCAGACCC
XM_005262126.1      ATGGGTGACAGAAGATTCATTGACTTCCAATTCCAAGATTCAAATTCAAGCCTCAGACCC
NM_001278000.1      ATGGGTGACAGAAGATTCATTGACTTCCAATTCCAAGATTCAAATTCAAGCCTCAGACCC
                    ************************************************************

HsCD00305000        AGGTTGGGCAATGCTACTGCCAATAATACTTGCATTGTTGATGATTCCTTCAAGTATAAT
NM_005296.2         AGGTTGGGCAATGCTACTGCCAATAATACTTGCATTGTTGATGATTCCTTCAAGTATAAT
XM_005262126.1      AGGTTGGGCAATGCTACTGCCAATAATACTTGCATTGTTGATGATTCCTTCAAGTATAAT
NM_001278000.1      AGGTTGGGCAATGCTACTGCCAATAATACTTGCATTGTTGATGATTCCTTCAAGTATAAT
                    ************************************************************

HsCD00305000        CTCAATGGTGCTGTCTACAGTGTTGTATTCATCTTGGGTCTGATAACCAACAGTGTCTCT
NM_005296.2         CTCAATGGTGCTGTCTACAGTGTTGTATTCATCTTGGGTCTGATAACCAACAGTGTCTCT
XM_005262126.1      CTCAATGGTGCTGTCTACAGTGTTGTATTCATCTTGGGTCTGATAACCAACAGTGTCTCT
NM_001278000.1      CTCAATGGTGCTGTCTACAGTGTTGTATTCATCTTGGGTCTGATAACCAACAGTGTCTCT
                    ************************************************************

HsCD00305000        CTGTTTGTCTTCTGTTTCCGCATGAAAATGAGAAGTGAGACTGCTATTTTTATCACCAAT
NM_005296.2         CTGTTTGTCTTCTGTTTCCGCATGAAAATGAGAAGTGAGACTGCTATTTTTATCACCAAT
XM_005262126.1      CTGTTTGTCTTCTGTTTCCGCATGAAAATGAGAAGTGAGACTGCTATTTTTATCACCAAT
NM_001278000.1      CTGTTTGTCTTCTGTTTCCGCATGAAAATGAGAAGTGAGACTGCTATTTTTATCACCAAT
                    ************************************************************

HsCD00305000        CTAGCTGTCTCTGATTTGCTTTTTGTCTGTACACTACCTTTTAAAATATTTTACAACTTC
NM_005296.2         CTAGCTGTCTCTGATTTGCTTTTTGTCTGTACACTACCTTTTAAAATATTTTACAACTTC
XM_005262126.1      CTAGCTGTCTCTGATTTGCTTTTTGTCTGTACACTACCTTTTAAAATATTTTACAACTTC
NM_001278000.1      CTAGCTGTCTCTGATTTGCTTTTTGTCTGTACACTACCTTTTAAAATATTTTACAACTTC
                    ************************************************************

HsCD00305000        AACCGCCACTGGCCTTTTGGTGACACCCTCTGCAAGATCTCTGGAACTGCATTCCTTACC
NM_005296.2         AACCGCCACTGGCCTTTTGGTGACACCCTCTGCAAGATCTCTGGAACTGCATTCCTTACC
XM_005262126.1      AACCGCCACTGGCCTTTTGGTGACACCCTCTGCAAGATCTCTGGAACTGCATTCCTTACC
NM_001278000.1      AACCGCCACTGGCCTTTTGGTGACACCCTCTGCAAGATCTCTGGAACTGCATTCCTTACC
                    ************************************************************

HsCD00305000        AACATCTATGGGAGCATGCTCTTTCTCACCTGTATTAGTGTGGATCGTTTCCTGGCCATT
NM_005296.2         AACATCTATGGGAGCATGCTCTTTCTCACCTGTATTAGTGTGGATCGTTTCCTGGCCATT
XM_005262126.1      AACATCTATGGGAGCATGCTCTTTCTCACCTGTATTAGTGTGGATCGTTTCCTGGCCATT
NM_001278000.1      AACATCTATGGGAGCATGCTCTTTCTCACCTGTATTAGTGTGGATCGTTTCCTGGCCATT
                    ************************************************************

HsCD00305000        GTCTATCCTTTTCGATCTCGTACTATTAGGACTAGGAGGAATTCTGCCATTGTGTGTGCT
NM_005296.2         GTCTATCCTTTTCGATCTCGTACTATTAGGACTAGGAGGAATTCTGCCATTGTGTGTGCT
XM_005262126.1      GTCTATCCTTTTCGATCTCGTACTATTAGGACTAGGAGGAATTCTGCCATTGTGTGTGCT
NM_001278000.1      GTCTATCCTTTTCGATCTCGTACTATTAGGACTAGGAGGAATTCTGCCATTGTGTGTGCT
                    ************************************************************

HsCD00305000        GGTGTCTGGATCCTAGTCCTCAGTGGCGGTATTTCAGCCTCTTTGTTTTCCACCACTAAT
NM_005296.2         GGTGTCTGGATCCTAGTCCTCAGTGGCGGTATTTCAGCCTCTTTGTTTTCCACCACTAAT
XM_005262126.1      GGTGTCTGGATCCTAGTCCTCAGTGGCGGTATTTCAGCCTCTTTGTTTTCCACCACTAAT
NM_001278000.1      GGTGTCTGGATCCTAGTCCTCAGTGGCGGTATTTCAGCCTCTTTGTTTTCCACCACTAAT
                    ************************************************************

HsCD00305000        GTCAACAATGCAACCACCACCTGCTTTGAAGGCTTCTCCAAACGTGTCTGGAAGACTTAT
NM_005296.2         GTCAACAATGCAACCACCACCTGCTTTGAAGGCTTCTCCAAACGTGTCTGGAAGACTTAT
XM_005262126.1      GTCAACAATGCAACCACCACCTGCTTTGAAGGCTTCTCCAAACGTGTCTGGAAGACTTAT
NM_001278000.1      GTCAACAATGCAACCACCACCTGCTTTGAAGGCTTCTCCAAACGTGTCTGGAAGACTTAT
                    ************************************************************

HsCD00305000        TTATCCAAGATCACAATATTTATTGAAGTTGTTGGGTTTATCATTCCTCTAATATTGAAT
NM_005296.2         TTATCCAAGATCACAATATTTATTGAAGTTGTTGGGTTTATCATTCCTCTAATATTGAAT
XM_005262126.1      TTATCCAAGATCACAATATTTATTGAAGTTGTTGGGTTTATCATTCCTCTAATATTGAAT
NM_001278000.1      TTATCCAAGATCACAATATTTATTGAAGTTGTTGGGTTTATCATTCCTCTAATATTGAAT
                    ************************************************************

HsCD00305000        GTCTCTTGCTCTTCTGTGGTGCTGAGAACTCTTCGCAAGCCTGCTACTCTGTCTCAAATT
NM_005296.2         GTCTCTTGCTCTTCTGTGGTGCTGAGAACTCTTCGCAAGCCTGCTACTCTGTCTCAAATT
XM_005262126.1      GTCTCTTGCTCTTCTGTGGTGCTGAGAACTCTTCGCAAGCCTGCTACTCTGTCTCAAATT
NM_001278000.1      GTCTCTTGCTCTTCTGTGGTGCTGAGAACTCTTCGCAAGCCTGCTACTCTGTCTCAAATT
                    ************************************************************

HsCD00305000        GGGACCAATAAGAAAAAAGTACTGAAAATGATCACAGTACATATGGCAGTCTTTGTGGTA
NM_005296.2         GGGACCAATAAGAAAAAAGTACTGAAAATGATCACAGTACATATGGCAGTCTTTGTGGTA
XM_005262126.1      GGGACCAATAAGAAAAAAGTACTGAAAATGATCACAGTACATATGGCAGTCTTTGTGGTA
NM_001278000.1      GGGACCAATAAGAAAAAAGTACTGAAAATGATCACAGTACATATGGCAGTCTTTGTGGTA
                    ************************************************************

HsCD00305000        TGCTTTGTACCCTACAACTCTGTCCTCTTCTTGTATGCCCTGGTGCGCTCCCAAGCTATT
NM_005296.2         TGCTTTGTACCCTACAACTCTGTCCTCTTCTTGTATGCCCTGGTGCGCTCCCAAGCTATT
XM_005262126.1      TGCTTTGTACCCTACAACTCTGTCCTCTTCTTGTATGCCCTGGTGCGCTCCCAAGCTATT
NM_001278000.1      TGCTTTGTACCCTACAACTCTGTCCTCTTCTTGTATGCCCTGGTGCGCTCCCAAGCTATT
                    ************************************************************

HsCD00305000        ACTAATTGCTTTTTGGAAAGATTTGCAAAGATCATGTACCCAATCACCTTGTGCCTTGCA
NM_005296.2         ACTAATTGCTTTTTGGAAAGATTTGCAAAGATCATGTACCCAATCACCTTGTGCCTTGCA
XM_005262126.1      ACTAATTGCTTTTTGGAAAGATTTGCAAAGATCATGTACCCAATCACCTTGTGCCTTGCA
NM_001278000.1      ACTAATTGCTTTTTGGAAAGATTTGCAAAGATCATGTACCCAATCACCTTGTGCCTTGCA
                    ************************************************************

HsCD00305000        ACTCTGAACTGTTGTTTTGACCCTTTCATCTATTACTTCACCCTTGAATCCTTTCAGAAG
NM_005296.2         ACTCTGAACTGTTGTTTTGACCCTTTCATCTATTACTTCACCCTTGAATCCTTTCAGAAG
XM_005262126.1      ACTCTGAACTGTTGTTTTGACCCTTTCATCTATTACTTCACCCTTGAATCCTTTCAGAAG
NM_001278000.1      ACTCTGAACTGTTGTTTTGACCCTTTCATCTATTACTTCACCCTTGAATCCTTTCAGAAG
                    ************************************************************

HsCD00305000        TCCTTCTACATCAATGCCCACATCAGAATGGAGTCCCTGTTTAAGACTGAAACACCTTTG
NM_005296.2         TCCTTCTACATCAATGCCCACATCAGAATGGAGTCCCTGTTTAAGACTGAAACACCTTTG
XM_005262126.1      TCCTTCTACATCAATGCCCACATCAGAATGGAGTCCCTGTTTAAGACTGAAACACCTTTG
NM_001278000.1      TCCTTCTACATCAATGCCCACATCAGAATGGAGTCCCTGTTTAAGACTGAAACACCTTTG
                    ************************************************************

HsCD00305000        ACCACAAAGCCTTCCCTTCCAGCTATTCAAGAGGAAGTGAGTGATCAAACAACAAATAAT
NM_005296.2         ACCACAAAGCCTTCCCTTCCAGCTATTCAAGAGGAAGTGAGTGATCAAACAACAAATAAT
XM_005262126.1      ACCACAAAGCCTTCCCTTCCAGCTATTCAAGAGGAAGTGAGTGATCAAACAACAAATAAT
NM_001278000.1      ACCACAAAGCCTTCCCTTCCAGCTATTCAAGAGGAAGTGAGTGATCAAACAACAAATAAT
                    ************************************************************

HsCD00305000        GGTGGTGAATTAATGCTAGAATCCACCTTTTAG
NM_005296.2         GGTGGTGAATTAATGCTAGAATCCACCTTTTAG
XM_005262126.1      GGTGGTGAATTAATGCTAGAATCCACCTTTTAG
NM_001278000.1      GGTGGTGAATTAATGCTAGAATCCACCTTTTAG
                    *********************************

Pathways

NCI : LPA receptor mediated events
NCI : LPA4-mediated signaling events
Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (q) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Gastrin-CREB signalling pathway via PKC and MAPK
Reactome : Nucleotide-like (purinergic) receptors
Reactome : P2Y receptors
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : GPCRs, Class A Rhodopsin-like
WikiPathway : Nucleotide GPCRs

Protein

HPRD : 02099

Clone

Cloning Information : 272097
HIP Master Clone ID : 202460
Original Clone ID : FLH272097.01L

Organism


TAX_ID : 9606
Species Specific ID: 2846

Genome

Chromosome : X
Map Location : Xq21.1
Ensembl : ENSG00000147145