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OPN3 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  OPN3
Gene Name:  opsin 3
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH272259.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: BMS
Description: None
Comments: Clone insert was fully sequence-verified by the clone submitter but the sequence is not available.
Mutation/
Discrepancy :
Unknown / Unknown
Publications: None
Authors: Bristol-Myers Squibb
HsCD00305162

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1209nts         Open reading frame : 1 to 1209
Reference Sequence Alignment

ATGTACTCGGGGAACCGCAGCGGCGGCCACGGCTACTGGGACGGCGGCGGGGCCGCGGGC
GCTGAGGGGCCGGCGCCGGCGGGGACACTGAGCCCCGCGCCCCTCTTCAGCCCCGGCACC
TACGAGCGCCTGGCGCTGCTGCTGGGCTCCATTGGGCTGCTGGGCGTCGGCAACAACCTG
CTGGTGCTCGTCCTCTACTACAAGTTCCAGCGGCTCCGCACTCCCACTCACCTCCTCCTG
GTCAACATCAGCCTCAGCGACCTGCTGGTGTCCCTCTTCGGGGTCACCTTTACCTTCGTG
TCCTGCCTGAGGAACGGCTGGGTGTGGGACACCGTGGGCTGCGTGTGGGACGGGTTTAGC
GGCAGCCTCTTCGGGATTGTTTCCATTGCCACCCTAACCGTGCTGGCCTATGAACGTTAC
ATTCGCGTGGTCCATGCCAGAGTGATCAATTTTTCCTGGGCCTGGAGGGCCATTACCTAC
ATCTGGCTCTACTCACTGGCGTGGGCAGGAGCACCTCTCCTGGGATGGAACAGGTACATC
CTGGACGTACACGGACTAGGCTGCACTGTGGACTGGAAATCCAAGGATGCCAACGATTCC
TCCTTTGTGCTTTTCTTATTTCTTGGCTGCCTGGTGGTGCCCCTGGGTGTCATAGCCCAT
TGCTATGGCCATATTCTATATTCCATTCGAATGCTTCGTTGTGTGGAAGATCTTCAGACA
ATTCAAGTGATCAAGATTTTAAAATATGAAAAGAAACTGGCCAAAATGTGCTTTTTAATG
ATATTCACCTTCCTGGTCTGTTGGATGCCTTATATCGTGATCTGCTTCTTGGTGGTTAAT
GGTCATGGTCACCTGGTCACTCCAACAATATCTATTGTTTCGTACCTCTTTGCTAAATCG
AACACTGTATACAATCCAGTGATTTATGTCTTCATGATCAGAAAGTTTCGAAGATCCCTT
TTGCAGCTTCTGTGCCTCCGACTGCTGAGGTGCCAGAGGCCTGCTAAAGACCTACCAGCA
GCTGGAAGTGAAATGCAGATCAGACCCATTGTGATGTCACAGAAAGATGGGGACAGGCCA
AAGAAAAAAGTGACTTTCAACTCTTCTTCCATCATTTTTATCATCACCAGTGATGAATCA
CTGTCAGTTGACGACAGCGACAAAACCAATGGGTCCAAAGTTGATGTAATCCAAGTTCGT
CCTTTGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1354
Start on reference sequence 146
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
(goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome

Gene

 

Gene Symbol : OPN3
Symbol Nomenclature : OPN3
SYNONYM : ECPN
Designation : encephalopsin|opsin 3 (encephalopsin, panopsin)|opsin-3
Full Nomenclature : opsin 3
GENEID : 23596
HGNC : 14007
MIM : 606695
Vega : OTTHUMG00000039691
Target GenBank: NM_014322

 

Reference Sequence Alignment

  

HsCD00305162      ATGTACTCGGGGAACCGCAGCGGCGGCCACGGCTACTGGGACGGCGGCGGGGCCGCGGGC
NM_014322.2       ATGTACTCGGGGAACCGCAGCGGCGGCCACGGCTACTGGGACGGCGGCGGGGCCGCGGGC
                  ************************************************************

HsCD00305162      GCTGAGGGGCCGGCGCCGGCGGGGACACTGAGCCCCGCGCCCCTCTTCAGCCCCGGCACC
NM_014322.2       GCTGAGGGGCCGGCGCCGGCGGGGACACTGAGCCCCGCGCCCCTCTTCAGCCCCGGCACC
                  ************************************************************

HsCD00305162      TACGAGCGCCTGGCGCTGCTGCTGGGCTCCATTGGGCTGCTGGGCGTCGGCAACAACCTG
NM_014322.2       TACGAGCGCCTGGCGCTGCTGCTGGGCTCCATTGGGCTGCTGGGCGTCGGCAACAACCTG
                  ************************************************************

HsCD00305162      CTGGTGCTCGTCCTCTACTACAAGTTCCAGCGGCTCCGCACTCCCACTCACCTCCTCCTG
NM_014322.2       CTGGTGCTCGTCCTCTACTACAAGTTCCAGCGGCTCCGCACTCCCACTCACCTCCTCCTG
                  ************************************************************

HsCD00305162      GTCAACATCAGCCTCAGCGACCTGCTGGTGTCCCTCTTCGGGGTCACCTTTACCTTCGTG
NM_014322.2       GTCAACATCAGCCTCAGCGACCTGCTGGTGTCCCTCTTCGGGGTCACCTTTACCTTCGTG
                  ************************************************************

HsCD00305162      TCCTGCCTGAGGAACGGCTGGGTGTGGGACACCGTGGGCTGCGTGTGGGACGGGTTTAGC
NM_014322.2       TCCTGCCTGAGGAACGGCTGGGTGTGGGACACCGTGGGCTGCGTGTGGGACGGGTTTAGC
                  ************************************************************

HsCD00305162      GGCAGCCTCTTCGGGATTGTTTCCATTGCCACCCTAACCGTGCTGGCCTATGAACGTTAC
NM_014322.2       GGCAGCCTCTTCGGGATTGTTTCCATTGCCACCCTAACCGTGCTGGCCTATGAACGTTAC
                  ************************************************************

HsCD00305162      ATTCGCGTGGTCCATGCCAGAGTGATCAATTTTTCCTGGGCCTGGAGGGCCATTACCTAC
NM_014322.2       ATTCGCGTGGTCCATGCCAGAGTGATCAATTTTTCCTGGGCCTGGAGGGCCATTACCTAC
                  ************************************************************

HsCD00305162      ATCTGGCTCTACTCACTGGCGTGGGCAGGAGCACCTCTCCTGGGATGGAACAGGTACATC
NM_014322.2       ATCTGGCTCTACTCACTGGCGTGGGCAGGAGCACCTCTCCTGGGATGGAACAGGTACATC
                  ************************************************************

HsCD00305162      CTGGACGTACACGGACTAGGCTGCACTGTGGACTGGAAATCCAAGGATGCCAACGATTCC
NM_014322.2       CTGGACGTACACGGACTAGGCTGCACTGTGGACTGGAAATCCAAGGATGCCAACGATTCC
                  ************************************************************

HsCD00305162      TCCTTTGTGCTTTTCTTATTTCTTGGCTGCCTGGTGGTGCCCCTGGGTGTCATAGCCCAT
NM_014322.2       TCCTTTGTGCTTTTCTTATTTCTTGGCTGCCTGGTGGTGCCCCTGGGTGTCATAGCCCAT
                  ************************************************************

HsCD00305162      TGCTATGGCCATATTCTATATTCCATTCGAATGCTTCGTTGTGTGGAAGATCTTCAGACA
NM_014322.2       TGCTATGGCCATATTCTATATTCCATTCGAATGCTTCGTTGTGTGGAAGATCTTCAGACA
                  ************************************************************

HsCD00305162      ATTCAAGTGATCAAGATTTTAAAATATGAAAAGAAACTGGCCAAAATGTGCTTTTTAATG
NM_014322.2       ATTCAAGTGATCAAGATTTTAAAATATGAAAAGAAACTGGCCAAAATGTGCTTTTTAATG
                  ************************************************************

HsCD00305162      ATATTCACCTTCCTGGTCTGTTGGATGCCTTATATCGTGATCTGCTTCTTGGTGGTTAAT
NM_014322.2       ATATTCACCTTCCTGGTCTGTTGGATGCCTTATATCGTGATCTGCTTCTTGGTGGTTAAT
                  ************************************************************

HsCD00305162      GGTCATGGTCACCTGGTCACTCCAACAATATCTATTGTTTCGTACCTCTTTGCTAAATCG
NM_014322.2       GGTCATGGTCACCTGGTCACTCCAACAATATCTATTGTTTCGTACCTCTTTGCTAAATCG
                  ************************************************************

HsCD00305162      AACACTGTATACAATCCAGTGATTTATGTCTTCATGATCAGAAAGTTTCGAAGATCCCTT
NM_014322.2       AACACTGTATACAATCCAGTGATTTATGTCTTCATGATCAGAAAGTTTCGAAGATCCCTT
                  ************************************************************

HsCD00305162      TTGCAGCTTCTGTGCCTCCGACTGCTGAGGTGCCAGAGGCCTGCTAAAGACCTACCAGCA
NM_014322.2       TTGCAGCTTCTGTGCCTCCGACTGCTGAGGTGCCAGAGGCCTGCTAAAGACCTACCAGCA
                  ************************************************************

HsCD00305162      GCTGGAAGTGAAATGCAGATCAGACCCATTGTGATGTCACAGAAAGATGGGGACAGGCCA
NM_014322.2       GCTGGAAGTGAAATGCAGATCAGACCCATTGTGATGTCACAGAAAGATGGGGACAGGCCA
                  ************************************************************

HsCD00305162      AAGAAAAAAGTGACTTTCAACTCTTCTTCCATCATTTTTATCATCACCAGTGATGAATCA
NM_014322.2       AAGAAAAAAGTGACTTTCAACTCTTCTTCCATCATTTTTATCATCACCAGTGATGAATCA
                  ************************************************************

HsCD00305162      CTGTCAGTTGACGACAGCGACAAAACCAATGGGTCCAAAGTTGATGTAATCCAAGTTCGT
NM_014322.2       CTGTCAGTTGACGACAGCGACAAAACCAATGGGTCCAAAGTTGATGTAATCCAAGTTCGT
                  ************************************************************

HsCD00305162      CCTTTGTAG
NM_014322.2       CCTTTGTAG
                  *********

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (i) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Opsins
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : GPCRs, Class A Rhodopsin-like

Protein

HPRD : 05985

Clone

Cloning Information : 272259
HIP Master Clone ID : 202622
Original Clone ID : FLH272259.01L

Organism


TAX_ID : 9606
Species Specific ID: 23596

Genome

Chromosome : 1
Map Location : 1q43
Ensembl : ENSG00000054277