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P2RY13 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  P2RY13
Gene Name:  purinergic receptor P2Y, G-protein coupled, 13
Sequence              Map: pANT7_cGST.doc
Original Clone ID: FLH272417.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: HIP
Description: None
Comments: Clone insert was fully sequence-verified by the clone submitter but the sequence is not available.
Mutation/
Discrepancy :
Unknown / Unknown
Publications: None
Authors:
HsCD00305487

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1002nts         Open reading frame : 1 to 1002
Reference Sequence Alignment

ATGAACACCACAGTGATGCAAGGCTTCAACAGATCTGAGCGGTGCCCCAGAGACACTCGG
ATAGTACAGCTGGTATTCCCAGCCCTCTACACAGTGGTTTTCTTGACCGGCATCCTGCTG
AATACTTTGGCTCTGTGGGTGTTTGTTCACATCCCCAGCTCCTCCACCTTCATCATCTAC
CTCAAAAACACTTTGGTGGCCGACTTGATAATGACACTCATGCTTCCTTTCAAAATCCTC
TCTGACTCACACCTGGCACCCTGGCAGCTCAGAGCTTTTGTGTGTCGTTTTTCTTCGGTG
ATATTTTATGAGACCATGTATGTGGGCATCGTGCTGTTAGGGCTCATAGCCTTTGACAGA
TTCCTCAAGATCATCAGACCTTTGAGAAATATTTTTCTAAAAAAACCTGTTTTTGCAAAA
ACGGTCTCAATCTTCATCTGGTTCTTTTTGTTCTTCATCTCCCTGCCAAATACGATCTTG
AGCAACAAGGAAGCAACACCATCGTCTGTGAAAAAGTGTGCTTCCTTAAAGGGGCCTCTG
GGGCTGAAATGGCATCAAATGGTAAATAACATATGCCAGTTTATTTTCTGGACTGTTTTT
ATCCTAATGCTTGTGTTTTATGTGGTTATTGCAAAAAAAGTATATGATTCTTATAGAAAG
TCCAAAAGTAAGGACAGAAAAAACAACAAAAAGCTGGAAGGCAAAGTATTTGTTGTCGTG
GCTGTCTTCTTTGTGTGTTTTGCTCCATTTCATTTTGCCAGAGTTCCATATACTCACAGT
CAAACCAACAATAAGACTGACTGTAGACTGCAAAATCAACTGTTTATTGCTAAAGAAACA
ACTCTCTTTTTGGCAGCAACTAACATTTGTATGGATCCCTTAATATACATATTCTTATGT
AAAAAATTCACAGAAAAGCTACCATGTATGCAAGGGAGAAAGACCACAGCATCAAGCCAA
GAAAATCATAGCAGTCAGACAGACAACATAACCTTAGGCTGA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1175
Start on reference sequence 174
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
(goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : P2RY13
Symbol Nomenclature : P2RY13
SYNONYM : GPCR1; GPR86; GPR94; P2Y13; SP174
Designation : G protein-coupled receptor 86|G-protein coupled receptor 86|G-protein coupled receptor 94|P2Y purinoceptor 13
Full Nomenclature : purinergic receptor P2Y, G-protein coupled, 13
GENEID : 53829
Locus Tag : FKSG77
HGNC : 4537
MIM : 606380
Vega : OTTHUMG00000155745
Target GenBank: NM_023914

 

Reference Sequence Alignment

  

HsCD00305487      ------------------------------------------------------------
NM_176894.2       ATGACTGCCGCCATAAGAAGACAGAGAGAACTGAGTATCCTCCCAAAGGTGACACTGGAA
                                                                              

HsCD00305487      ---ATGAACACCACAGTGATGCAAGGCTTCAACAGATCTGAGCGGTGCCCCAGAGACACT
NM_176894.2       GCAATGAACACCACAGTGATGCAAGGCTTCAACAGATCTGAGCGGTGCCCCAGAGACACT
                     *********************************************************

HsCD00305487      CGGATAGTACAGCTGGTATTCCCAGCCCTCTACACAGTGGTTTTCTTGACCGGCATCCTG
NM_176894.2       CGGATAGTACAGCTGGTATTCCCAGCCCTCTACACAGTGGTTTTCTTGACCGGCATCCTG
                  ************************************************************

HsCD00305487      CTGAATACTTTGGCTCTGTGGGTGTTTGTTCACATCCCCAGCTCCTCCACCTTCATCATC
NM_176894.2       CTGAATACTTTGGCTCTGTGGGTGTTTGTTCACATCCCCAGCTCCTCCACCTTCATCATC
                  ************************************************************

HsCD00305487      TACCTCAAAAACACTTTGGTGGCCGACTTGATAATGACACTCATGCTTCCTTTCAAAATC
NM_176894.2       TACCTCAAAAACACTTTGGTGGCCGACTTGATAATGACACTCATGCTTCCTTTCAAAATC
                  ************************************************************

HsCD00305487      CTCTCTGACTCACACCTGGCACCCTGGCAGCTCAGAGCTTTTGTGTGTCGTTTTTCTTCG
NM_176894.2       CTCTCTGACTCACACCTGGCACCCTGGCAGCTCAGAGCTTTTGTGTGTCGTTTTTCTTCG
                  ************************************************************

HsCD00305487      GTGATATTTTATGAGACCATGTATGTGGGCATCGTGCTGTTAGGGCTCATAGCCTTTGAC
NM_176894.2       GTGATATTTTATGAGACCATGTATGTGGGCATCGTGCTGTTAGGGCTCATAGCCTTTGAC
                  ************************************************************

HsCD00305487      AGATTCCTCAAGATCATCAGACCTTTGAGAAATATTTTTCTAAAAAAACCTGTTTTTGCA
NM_176894.2       AGATTCCTCAAGATCATCAGACCTTTGAGAAATATTTTTCTAAAAAAACCTGTTTTTGCA
                  ************************************************************

HsCD00305487      AAAACGGTCTCAATCTTCATCTGGTTCTTTTTGTTCTTCATCTCCCTGCCAAATACGATC
NM_176894.2       AAAACGGTCTCAATCTTCATCTGGTTCTTTTTGTTCTTCATCTCCCTGCCAAATACGATC
                  ************************************************************

HsCD00305487      TTGAGCAACAAGGAAGCAACACCATCGTCTGTGAAAAAGTGTGCTTCCTTAAAGGGGCCT
NM_176894.2       TTGAGCAACAAGGAAGCAACACCATCGTCTGTGAAAAAGTGTGCTTCCTTAAAGGGGCCT
                  ************************************************************

HsCD00305487      CTGGGGCTGAAATGGCATCAAATGGTAAATAACATATGCCAGTTTATTTTCTGGACTGTT
NM_176894.2       CTGGGGCTGAAATGGCATCAAATGGTAAATAACATATGCCAGTTTATTTTCTGGACTGTT
                  ************************************************************

HsCD00305487      TTTATCCTAATGCTTGTGTTTTATGTGGTTATTGCAAAAAAAGTATATGATTCTTATAGA
NM_176894.2       TTTATCCTAATGCTTGTGTTTTATGTGGTTATTGCAAAAAAAGTATATGATTCTTATAGA
                  ************************************************************

HsCD00305487      AAGTCCAAAAGTAAGGACAGAAAAAACAACAAAAAGCTGGAAGGCAAAGTATTTGTTGTC
NM_176894.2       AAGTCCAAAAGTAAGGACAGAAAAAACAACAAAAAGCTGGAAGGCAAAGTATTTGTTGTC
                  ************************************************************

HsCD00305487      GTGGCTGTCTTCTTTGTGTGTTTTGCTCCATTTCATTTTGCCAGAGTTCCATATACTCAC
NM_176894.2       GTGGCTGTCTTCTTTGTGTGTTTTGCTCCATTTCATTTTGCCAGAGTTCCATATACTCAC
                  ************************************************************

HsCD00305487      AGTCAAACCAACAATAAGACTGACTGTAGACTGCAAAATCAACTGTTTATTGCTAAAGAA
NM_176894.2       AGTCAAACCAACAATAAGACTGACTGTAGACTGCAAAATCAACTGTTTATTGCTAAAGAA
                  ************************************************************

HsCD00305487      ACAACTCTCTTTTTGGCAGCAACTAACATTTGTATGGATCCCTTAATATACATATTCTTA
NM_176894.2       ACAACTCTCTTTTTGGCAGCAACTAACATTTGTATGGATCCCTTAATATACATATTCTTA
                  ************************************************************

HsCD00305487      TGTAAAAAATTCACAGAAAAGCTACCATGTATGCAAGGGAGAAAGACCACAGCATCAAGC
NM_176894.2       TGTAAAAAATTCACAGAAAAGCTACCATGTATGCAAGGGAGAAAGACCACAGCATCAAGC
                  ************************************************************

HsCD00305487      CAAGAAAATCATAGCAGTCAGACAGACAACATAACCTTAGGCTGA
NM_176894.2       CAAGAAAATCATAGCAGTCAGACAGACAACATAACCTTAGGCTGA
                  *********************************************

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (i) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Nucleotide-like (purinergic) receptors
Reactome : P2Y receptors
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : GPCRs, Class A Rhodopsin-like
WikiPathway : GPCRs, Other

Protein

HPRD : 16210

Clone

Cloning Information : 272417
HIP Master Clone ID : 202362
Original Clone ID : FLH272417.01L

Organism


TAX_ID : 9606
Species Specific ID: 53829

Genome

Chromosome : 3
Map Location : 3q24
Ensembl : ENSG00000181631

Reagents

Labome : P2RY13-antibody