DNASU Plasmid Repository • 480.965.5697 | Email

Detailed Vector Information: pVP68K

TOP

Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pVP68K              
Source: Center for Eukaryotic Structural Genomics
Description: Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette.
Comments: None
Publications: PMID: 18988018
Title: Flexi vector cloning.
PMID: 19645454
Title: Kinetic and Structural Investigations into the allosteric and pH effect on Substrate Specificity of Human Epithelial 15-Lipoxygenase-2
PMID: 23653358
Title: Fusion of dioxygenase and lignin-binding domains in a novel secreted enzyme from cellulolytic Streptomyces sp. SirexAA-E.
PMID: 25854603
Title: "Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies"
Authors: Center for Eukaryotic Structural Genomics
University of Wisconsin
EvNO00306669

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI,Promega

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pVP68K

pVP68K in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  MBP Forward
Reverse:  T7 Terminator
Description: Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette.
Comments: Vector contains a lethal BarCat cassette that requires the strain BR610 for growth. Lethal cassette is removed during cloning.
Size (bp): 6353
Parent Vector: None
Properties: bacterial expression
Author Name: Center for Eukaryotic Structural Genomics
University of Wisconsin
Publications: PMID: 18988018
Title: Flexi vector cloning.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin of replication 4150 5377
gene fragment Barnase Barnase 1448 1780
primer T7 term T7 terminator reverse primer 2599 2616
primer MBP forward MBP forward primer site 1271 1291
promoter T5 T5 promoter region 122 202
protease cleavage site 3C 3C protease cleavage site 1389 1412
repressor binding site LacI LacI coding region 2980 4071
selectable marker CmR chloramphenicol acetyltransferase gene 1885 2544
selectable marker KanR Kanamycin resistance gene 5385 6179
tag MBP N-terminal Maltose Binding Protein tag 267 1363
tag His N-terminal 8xHis tag 237 260
trxn termination sequence rrnB rrnB transcription terminator 2796 2892

 

Other Link: SBKB Technology Portal
Species Specific ID: None
Target GenBank: None