Prkab1 (R. norvegicus ) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Prkab1
Gene Name:  protein kinase, AMP-activated, beta 1 non-catalytic subunit
Original Clone ID:
1234.1.77_GO.59083
Keyword:
full-length cds
Species:
Rattus norvegicus
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
This is a GATEWAY entry clone having a TEV protease cleavage site fused to the open reading frame with the initiation codon replaced with TCC.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
RnCD00313260
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 831nts
Open reading frame: 51 to 881

ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCGGCAATACGAGCAGCGAGCGCGCCGCGCTGGAGCGGCAGGCTGGCCATA
AGACGCCGCGGAGGGACAGCTCGGGGGGCACCAAGGATGGGGACAGGCCCAAGATCCTGA
TGGACAGCCCCGAAGACGCCGACATCTTCCACACCGAGGAAATGAAGGCTCCAGAGAAGG
AGGAGTTCCTGGCGTGGCAGCACGACCTCGAGGTGAATGAGAAAGCCCCCGCCCAGGCTC
GGCCCACCGTATTTCGATGGACAGGGGGTGGAAAGGAGGTCTACTTGTCTGGATCCTTCA
ACAACTGGAGCAAATTGCCCCTCACTAGAAGCCAAAACAACTTCGTAGCCATCCTGGACC
TGCCGGAAGGAGAGCATCAGTACAAGTTCTTTGTGGATGGCCAGTGGACCCACGATCCTT
CCGAGCCAATAGTAACCAGCCAGCTTGGCACAGTTAACAACATCATTCAAGTGAAGAAAA
CTGACTTTGAAGTATTTGATGCTTTAATGGTGGATTCCCAAAAGTGCTCCGATGTATCTG
AGCTGTCCAGTTCCCCCCCAGGACCCTACCACCAGGAGCCTTACATCTCTAAACCAGAGG
AGCGGTTCAAGGCCCCGCCCATCCTCCCGCCTCACCTGCTGCAGGTCATCTTGAACAAGG
ACACGGGCATCTCTTGTGATCCAGCGCTGCTTCCGGAGCCCAACCACGTCATGCTGAACC
ACCTCTATGCACTCTCTATCAAGGATGGAGTGATGGTGCTCAGTGCGACCCATCGGTACA
AGAAAAAGTACGTCACCACCCTCCTCTACAAGCCCATATGATAGGACCCAGCTTTCTTGT
ACAAAGTTGGCATTATAAGAAAGCATTGCTTA

Protein sequence predicted based on nucleotide sequence

ENLYFQSGNTSSERAALERQAGHKTPRRDSSGGTKDGDRPKILMDSPEDADIFHTEEMKA
PEKEEFLAWQHDLEVNEKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSQNNFVA
ILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCS
DVSELSSSPPGPYHQEPYISKPEERFKAPPILPPHLLQVILNKDTGISCDPALLPEPNHV
MLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
CCAACTTTGTACAAAAAAGCAGGCTCC
3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTTGGC

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.59083
Recommended expression in:
Not Applicable