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C19orf28 (H. sapiens ) in pLenti6.2/V5-DEST

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Explanation of Terms


Gene: Gene Symbol:  MFSD12
Gene Name:  major facilitator superfamily domain containing 12
Sequence             
Original Clone ID: FLH269154.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329419

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1416nts         Open reading frame : 1 to 1416
Reference Sequence Alignment

ATGGGCCCGGGACCCCCAGCGGCCGGAGCGGCGCCGTCCCCGCGGCCGCTGTCCCTGGTG
GCGCGGCTGAGCTACGCCGTGGGCCACTTCCTCAACGACCTGTGCGCGTCCATGTGGTTC
ACCTACCTGCTGCTCTACCTGCACTCGGTGCGCGCCTACAGCTCCCGCGGCGCGGGGCTG
CTGCTGCTGCTGGGCCAGGTGGCCGACGGGCTGTGCACACCGCTCGTGGGCTACGAGGCC
GACCGCGCCGCCAGCTGCTGCGCCCGCTACGGCACCGTCTGCGTCCTGCTGTCCTTCCCC
TTCATCTTCAGCCCCTGCCTGGGCTGTGGGGCGGCCACGCCCGAGTGGGCTGCCCTCCTC
TACTACGGCCCGTTCATCGTGATCTTCCAGTTTGGCTGGGCCTCCACACAGATCTCCCAC
CTCAGCCTCATCCCGGAGCTCGTCACCAACGACCATGAGAAGGTGGAGCTCACGGCACTC
AGGTATGCGTTCACCGTGGTGGCCAACATCACCGTCTACGGCGCCGCCTGGCTCCTGCTG
CACCTGCAGGGCTCGTCGCGGGTGGAGCCCACCCAAGACATCAGCATCAGCGACCAGCTG
GGGGGCCAGGACGTGCCCGTGTTCCGGAACCTGTCCCTGCTGGTGGTGGGTGTCGGCGCC
GTGTTCTCACTGCTATTCCACCTGGGCACCCGGGAGAGGCGCCGGCCGCATGCGGAGGAG
CCAGGCGAGCACACCCCCCTGTTGGCCCCTGCCACGGCCCAGCCCCTGCTGCTCTGGAAG
CACTGGCTCCGGGAGCCGGCTTTCTACCAGGTGGGCATACTGTACATGACCACCAGGCTC
ATCGTGAACCTGTCCCAGACCTACATGGCCATGTACCTCACCTACTCGCTCCACCTGCCC
AAGAAGTTCATCGCGACCATTCCCCTGGTGATGTACCTCAGCGGCTTCTTGTCCTCCTTC
CTCATGAAGCCCATCAACAAGTGCATTGGGAGGAACATGACCTACTTCTCAGGCCTCCTG
GTGATCCTGGCCTTTGCCGCCTGGGTGGCGCTGGCGGAGGGACTGGGTGTGGCCGTGTAC
GCAGCGGCTGTGCTGCTGGGTGCTGGCTGTGCCACCATCCTCGTCACCTCGCTGGCCATG
ACGGCCGACCTCATCGGTCCCCACACGAACAGCGGAGCGTTCGTGTACGGCTCCATGAGC
TTCTTGGATAAGGTGGCCAATGGGCTGGCAGTCATGGCCATCCAGAGCCTGCACCCTTGC
CCCTCAGAGCTCTGCTGCAGGGCCTGCGTGAGCTTTTACCACTGGGCGATGGTGGCTGTG
ACGGGCGGCGTGGGCGTGGCCGCTGCCCTGTGTCTCTGTAGCCTCCTGCTGTGGCCGACC
CGCCTGCGACGCTGGGACCGTGATGCCCGGCCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1576
Start on reference sequence 161
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MFSD12
Symbol Nomenclature : MFSD12
SYNONYM : C19orf28; PP3501
Designation : major facilitator superfamily domain-containing protein 12
Full Nomenclature : major facilitator superfamily domain containing 12
GENEID : 126321
HGNC : 28299
Vega : OTTHUMG00000180766
Target GenBank: BC036706

 

Reference Sequence Alignment

  

HsCD00329419        ATGGGCCCGGGACCCCCAGCGGCCGGAGCGGCGCCGTCCCCGCGGCCGCTGTCCCTGGTG
NM_001287529.1      ATGGGCCCGGGACCCCCAGCGGCCGGAGCGGCGCCGTCCCCGCGGCCGCTGTCCCTGGTG
NM_174983.4         ATGGGCCCGGGACCCCCAGCGGCCGGAGCGGCGCCGTCCCCGCGGCCGCTGTCCCTGGTG
                    ************************************************************

HsCD00329419        GCGCGGCTGAGCTACGCCGTGGGCCACTTCCTCAACGACCTGTGCGCGTCCATGTGGTTC
NM_001287529.1      GCGCGGCTGAGCTACGCCGTGGGCCACTTCCTCAACGACCTGTGCGCGTCCATGTGGTTC
NM_174983.4         GCGCGGCTGAGCTACGCCGTGGGCCACTTCCTCAACGACCTGTGCGCGTCCATGTGGTTC
                    ************************************************************

HsCD00329419        ACCTACCTGCTGCTCTACCTGCACTCGGTGCGCGCCTACAGCTCCCGCGGCGCGGGGCTG
NM_001287529.1      ACCTACCTGCTGCTCTACCTGCACTCGGTGCGCGCCTACAGCTCCCGCGGCGCGGGGCTG
NM_174983.4         ACCTACCTGCTGCTCTACCTGCACTCGGTGCGCGCCTACAGCTCCCGCGGCGCGGGGCTG
                    ************************************************************

HsCD00329419        CTGCTGCTGCTGGGCCAGGTGGCCGACGGGCTGTGCACACCGCTCGTGGGCTACGAGGCC
NM_001287529.1      CTGCTGCTGCTGGGCCAGGTGGCCGACGGGCTGTGCACACCGCTCGTGGGCTACGAGGCC
NM_174983.4         CTGCTGCTGCTGGGCCAGGTGGCCGACGGGCTGTGCACACCGCTCGTGGGCTACGAGGCC
                    ************************************************************

HsCD00329419        GACCGCGCCGCCAGCTGCTGCGCCCGCTACG---------------------------GC
NM_001287529.1      GACCGCGCCGCCAGCTGCTGCGCCCGCTACG---------------------------GC
NM_174983.4         GACCGCGCCGCCAGCTGCTGCGCCCGCTACGGCCCGCGCAAGGCCTGGCACCTGGTCGGC
                    *******************************                           **

HsCD00329419        ACCGTCTGCGTCCTGCTGTCCTTCCCCTTCATCTTCAGCCCCTGCCTGGGCTGTGGGGCG
NM_001287529.1      ACCGTCTGCGTCCTGCTGTCCTTCCCCTTCATCTTCAGCCCCTGCCTGGGCTGTGGGGCG
NM_174983.4         ACCGTCTGCGTCCTGCTGTCCTTCCCCTTCATCTTCAGCCCCTGCCTGGGCTGTGGGGCG
                    ************************************************************

HsCD00329419        GCCACGCCCGAGTGGGCTGCCCTCCTCTACTACGGCCCGTTCATCGTGATCTTCCAGTTT
NM_001287529.1      GCCACGCCCGAGTGGGCTGCCCTCCTCTACTACGGCCCGTTCATCGTGATCTTCCAGTTT
NM_174983.4         GCCACGCCCGAGTGGGCTGCCCTCCTCTACTACGGCCCGTTCATCGTGATCTTCCAGTTT
                    ************************************************************

HsCD00329419        GGCTGGGCCTCCACACAGATCTCCCACCTCAGCCTCATCCCGGAGCTCGTCACCAACGAC
NM_001287529.1      GGCTGGGCCTCCACACAGATCTCCCACCTCAGCCTCATCCCGGAGCTCGTCACCAACGAC
NM_174983.4         GGCTGGGCCTCCACACAGATCTCCCACCTCAGCCTCATCCCGGAGCTCGTCACCAACGAC
                    ************************************************************

HsCD00329419        CATGAGAAGGTGGAGCTCACGGCACTCAGGTATGCGTTCACCGTGGTGGCCAACATCACC
NM_001287529.1      CATGAGAAGGTGGAGCTCACGGCACTCAGGTATGCGTTCACCGTGGTGGCCAACATCACC
NM_174983.4         CATGAGAAGGTGGAGCTCACGGCACTCAGGTATGCGTTCACCGTGGTGGCCAACATCACC
                    ************************************************************

HsCD00329419        GTCTACGGCGCCGCCTGGCTCCTGCTGCACCTGCAGGGCTCGTCGCGGGTGGAGCCCACC
NM_001287529.1      GTCTACGGCGCCGCCTGGCTCCTGCTGCACCTGCAGGGCTCGTCGCGGGTGGAGCCCACC
NM_174983.4         GTCTACGGCGCCGCCTGGCTCCTGCTGCACCTGCAGGGCTCGTCGCGGGTGGAGCCCACC
                    ************************************************************

HsCD00329419        CAAGACATCAGCATCAGCGACCAGCTGGGGGGCCAGGACGTGCCCGTGTTCCGGAACCTG
NM_001287529.1      CAAGACATCAGCATCAGCGACCAGCTGGGGGGCCAGGACGTGCCCGTGTTCCGGAACCTG
NM_174983.4         CAAGACATCAGCATCAGCGACCAGCTGGGGGGCCAGGACGTGCCCGTGTTCCGGAACCTG
                    ************************************************************

HsCD00329419        TCCCTGCTGGTGGTGGGTGTCGGCGCCGTGTTCTCACTGCTATTCCACCTGGGCACCCGG
NM_001287529.1      TCCCTGCTGGTGGTGGGTGTCGGCGCCGTGTTCTCACTGCTATTCCACCTGGGCACCCGG
NM_174983.4         TCCCTGCTGGTGGTGGGTGTCGGCGCCGTGTTCTCACTGCTATTCCACCTGGGCACCCGG
                    ************************************************************

HsCD00329419        GAGAGGCGCCGGCCGCATGCGGAGGAGCCAGGCGAGCACACCCCCCTGTTGGCCCCTGCC
NM_001287529.1      GAGAGGCGCCGGCCGCATGCGGAGGAGCCAGGCGAGCACACCCCCCTGTTGGCCCCTGCC
NM_174983.4         GAGAGGCGCCGGCCGCATGCGGAGGAGCCAGGCGAGCACACCCCCCTGTTGGCCCCTGCC
                    ************************************************************

HsCD00329419        ACGGCCCAGCCCCTGCTGCTCTGGAAGCACTGGCTCCGGGAGCCGGCTTTCTACCAGGTG
NM_001287529.1      ACGGCCCAGCCCCTGCTGCTCTGGAAGCACTGGCTCCGGGAGCCGGCTTTCTACCAGGTG
NM_174983.4         ACGGCCCAGCCCCTGCTGCTCTGGAAGCACTGGCTCCGGGAGCCGGCTTTCTACCAGGTG
                    ************************************************************

HsCD00329419        GGCATACTGTACATGACCACCAGGCTCATCGTGAACCTGTCCCAGACCTACATGGCCATG
NM_001287529.1      GGCATACTGTACATGACCACCAGGCTCATCGTGAACCTGTCCCAGACCTACATGGCCATG
NM_174983.4         GGCATACTGTACATGACCACCAGGCTCATCGTGAACCTGTCCCAGACCTACATGGCCATG
                    ************************************************************

HsCD00329419        TACCTCACCTACTCGCTCCACCTGCCCAAGAAGTTCATCGCGACCATTCCCCTGGTGATG
NM_001287529.1      TACCTCACCTACTCGCTCCACCTGCCCAAGAAGTTCATCGCGACCATTCCCCTGGTGATG
NM_174983.4         TACCTCACCTACTCGCTCCACCTGCCCAAGAAGTTCATCGCGACCATTCCCCTGGTGATG
                    ************************************************************

HsCD00329419        TACCTCAGCGGCTTCTTGTCCTCCTTCCTCATGAAGCCCATCAACAAGTGCATTGGGAGG
NM_001287529.1      TACCTCAGCGGCTTCTTGTCCTCCTTCCTCATGAAGCCCATCAACAAGTGCATTGGGAGG
NM_174983.4         TACCTCAGCGGCTTCTTGTCCTCCTTCCTCATGAAGCCCATCAACAAGTGCATTGGGAGG
                    ************************************************************

HsCD00329419        AACATGACCTACTTCTCAGGCCTCCTGGTGATCCTGGCCTTTGCCGCCTGGGTGGCGCTG
NM_001287529.1      AACATGACCTACTTCTCAGGCCTCCTGGTGATCCTGGCCTTTGCCGCCTGGGTGGCGCTG
NM_174983.4         AACATGACCTACTTCTCAGGCCTCCTGGTGATCCTGGCCTTTGCCGCCTGGGTGGCGCTG
                    ************************************************************

HsCD00329419        GCGGAGGGACTGGGTGTGGCCGTGTACGCAGCGGCTGTGCTGCTGGGTGCTGGCTGTGCC
NM_001287529.1      GCGGAGGGACTGGGTGTGGCCGTGTACGCAGCGGCTGTGCTGCTGGGTGCTGGCTGTGCC
NM_174983.4         GCGGAGGGACTGGGTGTGGCCGTGTACGCAGCGGCTGTGCTGCTGGGTGCTGGCTGTGCC
                    ************************************************************

HsCD00329419        ACCATCCTCGTCACCTCGCTGGCCATGACGGCCGACCTCATCGGTCCCCACACGAACAGC
NM_001287529.1      ACCATCCTCGTCACCTCGCTGGCCATGACGGCCGACCTCATCGGTCCCCACACGAACAGC
NM_174983.4         ACCATCCTCGTCACCTCGCTGGCCATGACGGCCGACCTCATCGGTCCCCACACGAACAGC
                    ************************************************************

HsCD00329419        GGAGCGTTCGTGTACGGCTCCATGAGCTTCTTGGATAAGGTGGCCAATGGGCTGGCAGTC
NM_001287529.1      GGAGCGTTCGTGTACGGCTCCATGAGCTTCTTGGATAAGGTGGCCAATGGGCTGGCAGTC
NM_174983.4         GGAGCGTTCGTGTACGGCTCCATGAGCTTCTTGGATAAGGTGGCCAATGGGCTGGCAGTC
                    ************************************************************

HsCD00329419        ATGGCCATCCAGAGCCTGCACCCTTGCCCCTCAGAGCTCTGCTGCAGGGCCTGCGTGAGC
NM_001287529.1      ATGGCCATCCAGAGCCTGCACCCTTGCCCCTCAGAGCTCTGCTGCAGGGCCTGCGTGAGC
NM_174983.4         ATGGCCATCCAGAGCCTGCACCCTTGCCCCTCAGAGCTCTGCTGCAGGGCCTGCGTGAGC
                    ************************************************************

HsCD00329419        TTTTACCACTGGGCGATGGTGGCTGTGACGGGCGGCGTGGGCGTGGCCGCTGCCCTGTGT
NM_001287529.1      TTTTACCACTGGGCGATGGTGGCTGTGACGGGCGGCGTGGGCGTGGCCGCTGCCCTGTGT
NM_174983.4         TTTTACCACTGGGCGATGGTGGCTGTGACGGGCGGCGTGGGCGTGGCCGCTGCCCTGTGT
                    ************************************************************

HsCD00329419        CTCTGTAGCCTCCTGCTGTGGCCGACCCGCCTGCGACGCTGGGACCGTGATGCCCGGCCC
NM_001287529.1      CTCTGTAGCCTCCTGCTGTGGCCGACCCGCCTGCGACGCTGGGACCGTGATGCCCGGCCC
NM_174983.4         CTCTGTAGCCTCCTGCTGTGGCCGACCCGCCTGCGACGCTGGGACCGTGATGCCCGGCCC
                    ************************************************************

HsCD00329419        TTG
NM_001287529.1      TGA
NM_174983.4         TGA
                    * .

Protein

UniProt : Q3U481
HPRD : 12704

Clone

Cloning Information : 269154
HIP Master Clone ID : 159790
Original Clone ID : FLH269154.01L

Organism


TAX_ID : 9606
Species Specific ID: 73822

Genome

Chromosome : 19
Map Location : 19p13.3
Ensembl : ENSG00000161091

Reagents

Labome : MFSD12-antibody