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RNPS1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RNPS1
Gene Name:  RNA binding protein S1, serine-rich domain
Sequence             
Original Clone ID: FLH269158.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329423

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 918nts         Open reading frame : 1 to 918

ATGGATTTATCAGGAGTGAAAAAGAAGAGCTTGCTAGGAGTCAAAGAAAATAATAAAAAG
TCCAGCACTAGGGCTCCTTCACCTACCAAACGCAAAGACCGCTCAGATGAGAAGTCCAAG
GATCGCTCAAAAGATAAAGGGGCCACCAAGGAGTCGAGTGAGAAGGATCGCGGCCGGGAC
AAAACCCGAAAGAGGCGCAGCGCTTCCAGTGGTAGCAGCAGTACCAGGTCTCGGTCCAGC
TCGACTTCCAGCTCAGGCTCCAGCACCAGCACTGGCTCAAGCAGTGGCTCCAGCTCTTCC
TCAGCATCCAGCCGCTCAGGAAGCTCCAGCACCTCCCGCAGCTCCAGCTCTAGCAGCTCT
TCTGGCTCTCCAAGTCCTTCTCGGCGCAGACACGACAACAGGAGGCGCTCCCGCTCCAAA
TCCAAACCACCTAAAAGAGATGAAAAGGAGAGGAAAAGGCGGAGCCCATCTCCTAAGCCC
ACCAAAGTGCACATTGGGAGACTCACCCGGAATGTGACAAAGGATCACATCATGGAGATA
TTTTCCACCTATGGGAAAATTAAAATGATTGACATGCCCGTGGAAAGGATGCATCCCCAT
CTGTCCAAAGGCTATGCGTACGTAGAGTTTGAGAATCCAGATGAAGCCGAGAAGGCGCTG
AAGCACATGGATGGAGGACAAATTGATGGCCAGGAGATCACTGCCACCGCCGTGCTGGCC
CCCTGGCCTAGGCCACCCCCCAGGAGATTCAGCCCTCCCAGGAGAATGTTGCCACCACCG
CCTATGTGGCGCAGGTCTCCCCCACGGATGAGGAGAAGGTCCCGCTCCCCGAGGCGCAGG
TCCCCCGTGCGCCGGAGATCACGGTCCCCGGGCCGCCGCCGCCACAGGAGCCGCTCCAGC
TCCAACTCCTCCCGATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1092
Start on reference sequence 175
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RNPS1
Symbol Nomenclature : RNPS1
SYNONYM : E5.1
Designation : RNA-binding protein S1, serine-rich domain|RNA-binding protein with serine-rich domain 1|SR protein|SR-related protein LDC2
Full Nomenclature : RNA binding protein S1, serine-rich domain
GENEID : 10921
HGNC : 10080
MIM : 606447
Vega : OTTHUMG00000128828
Target GenBank: BC001659

 

Pathways

Reactome : Cleavage of Growing Transcript in the Termination Region
Reactome : Gene Expression
Reactome : Metabolism of RNA
Reactome : Metabolism of mRNA
Reactome : Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)
Reactome : Nonsense-Mediated Decay (NMD)
Reactome : Post-Elongation Processing of Intron-Containing pre-mRNA
Reactome : Post-Elongation Processing of the Transcript
Reactome : Processing of Capped Intron-Containing Pre-mRNA
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Termination
Reactome : Transcription
Reactome : Transport of Mature Transcript to Cytoplasm
Reactome : Transport of Mature mRNA derived from an Intron-Containing Transcript
Reactome : mRNA 3'-end processing
Reactome : mRNA Splicing
Reactome : mRNA Splicing - Major Pathway
WikiPathway : mRNA processing

Protein

SMART domain : RRM : RNA recognition motif
UniProt : Q15287
UniProt : D3DU92
HPRD : 07341

Clone

Cloning Information : 269158
HIP Master Clone ID : 160091
Original Clone ID : FLH269158.01L

Organism


TAX_ID : 9606
Species Specific ID: 10921

Genome

Chromosome : 16
Map Location : 16p13.3
Ensembl : ENSG00000205937

Reagents

Labome : RNPS1-antibody