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AATF (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  AATF
Gene Name:  apoptosis antagonizing transcription factor
Sequence             
Original Clone ID: FLH269170.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329435

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1683nts         Open reading frame : 1 to 1683
Reference Sequence Alignment

ATGGCGGGGCCGCAGCCCCTGGCGCTGCAACTGGAACAGTTGTTGAACCCGCGACCAAGC
GAGGCGGACCCTGAAGCGGACCCCGAGGAAGCCACTGCTGCCAGGGTGATTGACAGGTTT
GATGAAGGGGAAGATGGGGAAGGTGATTTCCTAGTAGTGGGTAGCATTAGAAAACTGGCA
TCAGCCTCCCTCTTGGACACGGACAAAAGGTATTGCGGCAAAACCACCTCTAGAAAAGCA
TGGAATGAAGACCATTGGGAGCAGACTCTGCCAGGATCGTCTGATGAGGAAATATCTGAT
GAGGAAGGGTCTGGAGATGAAGATTCAGAGGGACTGGGTCTGGAGGAATATGATGAGGAC
GACCTGGGTGCTGCTGAGGAACAGGAGTGTGGTGATCACAGGGAGAGCAAGAAGAGCAGA
AGCCACTCTGCAAAAACACCGGGCTTCAGTGTCCAGAGTATCAGTGACTTTGAGAAATTT
ACCAAGGGAATGGATGACCTTGGGAGCAGTGAGGAGGAGGAAGACGAAGAGAGTGGCATG
GAAGAAGGGGATGACGCGGAAGACTCCCAAGGCGAGAGTGAGGAAGACAGGGCTGGAGAT
AGAAACAGTGAGGATGATGGTGTGGTGATGACCTTCTCTAGTGTCAAAGTTTCTGAGGAA
GTGGAGAAAGGAAGAGCCGTGAAGAACCAGATAGCACTGTGGGACCAGCTCTTGGAAGGA
AGGATCAAACTACAAAAAGCTCTGTTGACCACCAACCAGCTTCCTCAACCAGATGTTTTC
CCATTGTTCAAGGACAAAGGTGGCCCAGAATTTTCCAGTGCCCTGAAAAATAGTCACAAG
GCACTTAAAGCATTGTTGAGGTCATTGGTAGGTCTTCAGGAAGAGTTGCTTTTCCAGTAC
CCAGACACTAGATATCTAGTAGATGGGACAAAGCCCAATGCGGGAAGTGAGGAGATTTCT
AGTGAAGATGATGAGCTGGTAGAAGAGAAGAAGCAGCAACGAAGAAGGGTCCCTGCAAAG
AGGAAGCTGGAGATGGAGGACTATCCCAGCTTCATGGCAAAGCGCTTTGCCGACTTTACA
GTCTACAGGAACCGCACACTTCAGAAATGGCACGATAAGACCAAACTGGCTTCTGGAAAA
CTGGGGAAGGGTTTTGGTGCCTTTGAACGCTCAATCTTGACTCAGATCGACCATATTCTG
ATGGACAAAGAGAGATTACTTCGAAGGACACAGACCAAGCGCTCTGTCTATCGAGTTCTT
GGCAAACCTGAGCCAGCAGCTCAGCCTGTCCCAGAGAGTTTGCCAGGGGAACCGGAGATC
CTTCCTCAAGCCCCTGCTAATGCTCATCTGAAGGACTTGGATGAAGAAATCTTTGATGAT
GATGACTTTTACCACCAGCTCCTTCGAGAACTCATAGAACGGAAGACCAGCTCCTTGGAT
CCCAACGATCAGGTGGCCATGGGAAGGCAGTGGCTTGCAATCCAGAAGTTACGAAGCAAA
ATCCACAAAAAAGTAGATAGGAAAGCCAGCAAAGGCAGGAAACTTCGGTTTCATGTCCTT
AGCAAGCTACTGAGTTTCATGGCACCTATTGACCATACTACAATGAATGATGATGCCAGG
ACAGAACTGTACCGCTCTCTTTTTGGCCAGCTCCACCCTCCCGACGAAGGCCACGGGGAT
TTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1823
Start on reference sequence 141
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : AATF
Symbol Nomenclature : AATF
SYNONYM : BFR2; CHE-1; CHE1; DED
Designation : apoptosis-antagonizing transcription factor|protein AATF|rb-binding protein Che-1
Full Nomenclature : apoptosis antagonizing transcription factor
GENEID : 26574
Locus Tag : HSPC277
HGNC : 19235
MIM : 608463
Vega : OTTHUMG00000179145
Target GenBank: BC000591

 

Reference Sequence Alignment

  

HsCD00329435      ATGGCGGGGCCGCAGCCCCTGGCGCTGCAACTGGAACAGTTGTTGAACCCGCGACCAAGC
NM_012138.3       ATGGCGGGGCCGCAGCCCCTGGCGCTGCAACTGGAACAGTTGTTGAACCCGCGACCAAGC
                  ************************************************************

HsCD00329435      GAGGCGGACCCTGAAGCGGACCCCGAGGAAGCCACTGCTGCCAGGGTGATTGACAGGTTT
NM_012138.3       GAGGCGGACCCTGAAGCGGACCCCGAGGAAGCCACTGCTGCCAGGGTGATTGACAGGTTT
                  ************************************************************

HsCD00329435      GATGAAGGGGAAGATGGGGAAGGTGATTTCCTAGTAGTGGGTAGCATTAGAAAACTGGCA
NM_012138.3       GATGAAGGGGAAGATGGGGAAGGTGATTTCCTAGTAGTGGGTAGCATTAGAAAACTGGCA
                  ************************************************************

HsCD00329435      TCAGCCTCCCTCTTGGACACGGACAAAAGGTATTGCGGCAAAACCACCTCTAGAAAAGCA
NM_012138.3       TCAGCCTCCCTCTTGGACACGGACAAAAGGTATTGCGGCAAAACCACCTCTAGAAAAGCA
                  ************************************************************

HsCD00329435      TGGAATGAAGACCATTGGGAGCAGACTCTGCCAGGATCGTCTGATGAGGAAATATCTGAT
NM_012138.3       TGGAATGAAGACCATTGGGAGCAGACTCTGCCAGGATCGTCTGATGAGGAAATATCTGAT
                  ************************************************************

HsCD00329435      GAGGAAGGGTCTGGAGATGAAGATTCAGAGGGACTGGGTCTGGAGGAATATGATGAGGAC
NM_012138.3       GAGGAAGGGTCTGGAGATGAAGATTCAGAGGGACTGGGTCTGGAGGAATATGATGAGGAC
                  ************************************************************

HsCD00329435      GACCTGGGTGCTGCTGAGGAACAGGAGTGTGGTGATCACAGGGAGAGCAAGAAGAGCAGA
NM_012138.3       GACCTGGGTGCTGCTGAGGAACAGGAGTGTGGTGATCACAGGGAGAGCAAGAAGAGCAGA
                  ************************************************************

HsCD00329435      AGCCACTCTGCAAAAACACCGGGCTTCAGTGTCCAGAGTATCAGTGACTTTGAGAAATTT
NM_012138.3       AGCCACTCTGCAAAAACACCGGGCTTCAGTGTCCAGAGTATCAGTGACTTTGAGAAATTT
                  ************************************************************

HsCD00329435      ACCAAGGGAATGGATGACCTTGGGAGCAGTGAGGAGGAGGAAGACGAAGAGAGTGGCATG
NM_012138.3       ACCAAGGGAATGGATGACCTTGGGAGCAGTGAGGAGGAGGAAGACGAAGAGAGTGGCATG
                  ************************************************************

HsCD00329435      GAAGAAGGGGATGACGCGGAAGACTCCCAAGGCGAGAGTGAGGAAGACAGGGCTGGAGAT
NM_012138.3       GAAGAAGGGGATGACGCGGAAGACTCCCAAGGCGAGAGTGAGGAAGACAGGGCTGGAGAT
                  ************************************************************

HsCD00329435      AGAAACAGTGAGGATGATGGTGTGGTGATGACCTTCTCTAGTGTCAAAGTTTCTGAGGAA
NM_012138.3       AGAAACAGTGAGGATGATGGTGTGGTGATGACCTTCTCTAGTGTCAAAGTTTCTGAGGAA
                  ************************************************************

HsCD00329435      GTGGAGAAAGGAAGAGCCGTGAAGAACCAGATAGCACTGTGGGACCAGCTCTTGGAAGGA
NM_012138.3       GTGGAGAAAGGAAGAGCCGTGAAGAACCAGATAGCACTGTGGGACCAGCTCTTGGAAGGA
                  ************************************************************

HsCD00329435      AGGATCAAACTACAAAAAGCTCTGTTGACCACCAACCAGCTTCCTCAACCAGATGTTTTC
NM_012138.3       AGGATCAAACTACAAAAAGCTCTGTTGACCACCAACCAGCTTCCTCAACCAGATGTTTTC
                  ************************************************************

HsCD00329435      CCATTGTTCAAGGACAAAGGTGGCCCAGAATTTTCCAGTGCCCTGAAAAATAGTCACAAG
NM_012138.3       CCATTGTTCAAGGACAAAGGTGGCCCAGAATTTTCCAGTGCCCTGAAAAATAGTCACAAG
                  ************************************************************

HsCD00329435      GCACTTAAAGCATTGTTGAGGTCATTGGTAGGTCTTCAGGAAGAGTTGCTTTTCCAGTAC
NM_012138.3       GCACTTAAAGCATTGTTGAGGTCATTGGTAGGTCTTCAGGAAGAGTTGCTTTTCCAGTAC
                  ************************************************************

HsCD00329435      CCAGACACTAGATATCTAGTAGATGGGACAAAGCCCAATGCGGGAAGTGAGGAGATTTCT
NM_012138.3       CCAGACACTAGATATCTAGTAGATGGGACAAAGCCCAATGCGGGAAGTGAGGAGATTTCT
                  ************************************************************

HsCD00329435      AGTGAAGATGATGAGCTGGTAGAAGAGAAGAAGCAGCAACGAAGAAGGGTCCCTGCAAAG
NM_012138.3       AGTGAAGATGATGAGCTGGTAGAAGAGAAGAAGCAGCAACGAAGAAGGGTCCCTGCAAAG
                  ************************************************************

HsCD00329435      AGGAAGCTGGAGATGGAGGACTATCCCAGCTTCATGGCAAAGCGCTTTGCCGACTTTACA
NM_012138.3       AGGAAGCTGGAGATGGAGGACTATCCCAGCTTCATGGCAAAGCGCTTTGCCGACTTTACA
                  ************************************************************

HsCD00329435      GTCTACAGGAACCGCACACTTCAGAAATGGCACGATAAGACCAAACTGGCTTCTGGAAAA
NM_012138.3       GTCTACAGGAACCGCACACTTCAGAAATGGCACGATAAGACCAAACTGGCTTCTGGAAAA
                  ************************************************************

HsCD00329435      CTGGGGAAGGGTTTTGGTGCCTTTGAACGCTCAATCTTGACTCAGATCGACCATATTCTG
NM_012138.3       CTGGGGAAGGGTTTTGGTGCCTTTGAACGCTCAATCTTGACTCAGATCGACCATATTCTG
                  ************************************************************

HsCD00329435      ATGGACAAAGAGAGATTACTTCGAAGGACACAGACCAAGCGCTCTGTCTATCGAGTTCTT
NM_012138.3       ATGGACAAAGAGAGATTACTTCGAAGGACACAGACCAAGCGCTCTGTCTATCGAGTTCTT
                  ************************************************************

HsCD00329435      GGCAAACCTGAGCCAGCAGCTCAGCCTGTCCCAGAGAGTTTGCCAGGGGAACCGGAGATC
NM_012138.3       GGCAAACCTGAGCCAGCAGCTCAGCCTGTCCCAGAGAGTTTGCCAGGGGAACCGGAGATC
                  ************************************************************

HsCD00329435      CTTCCTCAAGCCCCTGCTAATGCTCATCTGAAGGACTTGGATGAAGAAATCTTTGATGAT
NM_012138.3       CTTCCTCAAGCCCCTGCTAATGCTCATCTGAAGGACTTGGATGAAGAAATCTTTGATGAT
                  ************************************************************

HsCD00329435      GATGACTTTTACCACCAGCTCCTTCGAGAACTCATAGAACGGAAGACCAGCTCCTTGGAT
NM_012138.3       GATGACTTTTACCACCAGCTCCTTCGAGAACTCATAGAACGGAAGACCAGCTCCTTGGAT
                  ************************************************************

HsCD00329435      CCCAACGATCAGGTGGCCATGGGAAGGCAGTGGCTTGCAATCCAGAAGTTACGAAGCAAA
NM_012138.3       CCCAACGATCAGGTGGCCATGGGAAGGCAGTGGCTTGCAATCCAGAAGTTACGAAGCAAA
                  ************************************************************

HsCD00329435      ATCCACAAAAAAGTAGATAGGAAAGCCAGCAAAGGCAGGAAACTTCGGTTTCATGTCCTT
NM_012138.3       ATCCACAAAAAAGTAGATAGGAAAGCCAGCAAAGGCAGGAAACTTCGGTTTCATGTCCTT
                  ************************************************************

HsCD00329435      AGCAAGCTACTGAGTTTCATGGCACCTATTGACCATACTACAATGAATGATGATGCCAGG
NM_012138.3       AGCAAGCTACTGAGTTTCATGGCACCTATTGACCATACTACAATGAATGATGATGCCAGG
                  ************************************************************

HsCD00329435      ACAGAACTGTACCGCTCTCTTTTTGGCCAGCTCCACCCTCCCGACGAAGGCCACGGGGAT
NM_012138.3       ACAGAACTGTACCGCTCTCTTTTTGGCCAGCTCCACCCTCCCGACGAAGGCCACGGGGAT
                  ************************************************************

HsCD00329435      TTG
NM_012138.3       TGA
                  * .

Pathways

NCI : Regulation of retinoblastoma protein
Reactome : Cell death signalling via NRAGE, NRIF and NADE
Reactome : NRAGE signals death through JNK
Reactome : Signal Transduction
Reactome : Signalling by NGF
Reactome : p75 NTR receptor-mediated signalling

Protein

UniProt : Q9NY61
HPRD : 12236

Clone

Cloning Information : 269170
HIP Master Clone ID : 163586
Original Clone ID : FLH269170.01L

Organism


TAX_ID : 9606
Species Specific ID: 26574

Genome

Chromosome : 17
Map Location : 17q12
Ensembl : ENSG00000108270

Reagents

Labome : AATF-antibody