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WWTR1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  WWTR1
Gene Name:  WW domain containing transcription regulator 1
Sequence             
Original Clone ID: FLH269178.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329443

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1203nts         Open reading frame : 1 to 1203
Reference Sequence Alignment

ATGAATCCGGCCTCGGCGCCCCCTCCGCTCCCGCCGCCTGGGCAGCAAGTGATCCACGTC
ACGCAGGACCTAGACACAGACCTCGAAGCCCTCTTCAACTCTGTCATGAATCCGAAGCCT
AGCTCGTGGCGGAAGAAGATCCTGCCGGAGTCTTTCTTTAAGGAGCCTGATTCGGGCTCG
CACTCGCGCCAGTCCAGCACCGACTCGTCGGGCGGCCACCCGGGGCCTCGACTGGCTGGG
GGTGCCCAGCATGTCCGCTCGCACTCGTCGCCCGCGTCCCTGCAGCTGGGCACCGGCGCG
GGTGCTGCGGGTAGCCCCGCGCAGCAGCACGCGCACCTCCGCCAGCAGTCCTACGACGTG
ACCGACGAGCTGCCACTGCCCCCGGGCTGGGAGATGACCTTCACGGCCACTGGCCAGAGG
TACTTCCTCAATCACATAGAAAAAATCACCACATGGCAAGACCCTAGGAAGGCGATGAAT
CAGCCTCTGAATCATATGAACCTCCACCCTGCCGTCAGTTCCACACCAGTGCCTCAGAGG
TCCATGGCAGTATCCCAGCCAAATCTCGTGATGAATCACCAACACCAGCAGCAGATGGCC
CCCAGTACCCTGAGCCAGCAGAACCACCCCACTCAGAACCCACCCGCAGGGCTCATGAGT
ATGCCCAATGCGCTGACCACTCAGCAGCAGCAGCAGCAGAAACTGCGGCTTCAGAGAATC
CAGATGGAGAGAGAAAGGATTCGAATGCGCCAAGAGGAGCTCATGAGGCAGGAAGCTGCC
CTCTGTCGACAGCTCCCCATGGAAGCTGAGACTCTTGCCCCAGTTCAGGCTGCTGTCAAC
CCACCCACGATGACCCCAGACATGAGATCCATCACTAATAATAGCTCAGATCCTTTCCTC
AATGGAGGGCCATATCATTCGAGGGAGCAGAGCACTGACAGTGGCCTGGGGTTAGGGTGC
TACAGTGTCCCCACAACTCCGGAGGACTTCCTCAGCAATGTGGATGAGATGGATACAGGA
GAAAACGCAGGACAAACACCCATGAACATCAATCCCCAACAGACCCGTTTCCCTGATTTC
CTTGACTGTCTTCCAGGAACAAACGTTGACTTAGGAACTTTGGAATCTGAAGACCTGATC
CCCCTCTTCAATGATGTAGAGTCTGCTCTGAACAAAAGTGAGCCCTTTCTAACCTGGCTG
TTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1446
Start on reference sequence 244
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : WWTR1
Symbol Nomenclature : WWTR1
SYNONYM : TAZ
Designation : WW domain-containing transcription regulator protein 1|transcriptional co-activator with PDZ-binding motif|transcriptional coactivator with PDZ-binding motif
Full Nomenclature : WW domain containing transcription regulator 1
GENEID : 25937
HGNC : 24042
MIM : 607392
Vega : OTTHUMG00000159614
Target GenBank: BC014052

 

Reference Sequence Alignment

  

HsCD00329443        ATGAATCCGGCCTCGGCGCCCCCTCCGCTCCCGCCGCCTGGGCAGCAAGTGATCCACGTC
NM_001168278.1      ATGAATCCGGCCTCGGCGCCCCCTCCGCTCCCGCCGCCTGGGCAGCAAGTGATCCACGTC
NM_015472.4         ATGAATCCGGCCTCGGCGCCCCCTCCGCTCCCGCCGCCTGGGCAGCAAGTGATCCACGTC
NM_001168280.1      ATGAATCCGGCCTCGGCGCCCCCTCCGCTCCCGCCGCCTGGGCAGCAAGTGATCCACGTC
                    ************************************************************

HsCD00329443        ACGCAGGACCTAGACACAGACCTCGAAGCCCTCTTCAACTCTGTCATGAATCCGAAGCCT
NM_001168278.1      ACGCAGGACCTAGACACAGACCTCGAAGCCCTCTTCAACTCTGTCATGAATCCGAAGCCT
NM_015472.4         ACGCAGGACCTAGACACAGACCTCGAAGCCCTCTTCAACTCTGTCATGAATCCGAAGCCT
NM_001168280.1      ACGCAGGACCTAGACACAGACCTCGAAGCCCTCTTCAACTCTGTCATGAATCCGAAGCCT
                    ************************************************************

HsCD00329443        AGCTCGTGGCGGAAGAAGATCCTGCCGGAGTCTTTCTTTAAGGAGCCTGATTCGGGCTCG
NM_001168278.1      AGCTCGTGGCGGAAGAAGATCCTGCCGGAGTCTTTCTTTAAGGAGCCTGATTCGGGCTCG
NM_015472.4         AGCTCGTGGCGGAAGAAGATCCTGCCGGAGTCTTTCTTTAAGGAGCCTGATTCGGGCTCG
NM_001168280.1      AGCTCGTGGCGGAAGAAGATCCTGCCGGAGTCTTTCTTTAAGGAGCCTGATTCGGGCTCG
                    ************************************************************

HsCD00329443        CACTCGCGCCAGTCCAGCACCGACTCGTCGGGCGGCCACCCGGGGCCTCGACTGGCTGGG
NM_001168278.1      CACTCGCGCCAGTCCAGCACCGACTCGTCGGGCGGCCACCCGGGGCCTCGACTGGCTGGG
NM_015472.4         CACTCGCGCCAGTCCAGCACCGACTCGTCGGGCGGCCACCCGGGGCCTCGACTGGCTGGG
NM_001168280.1      CACTCGCGCCAGTCCAGCACCGACTCGTCGGGCGGCCACCCGGGGCCTCGACTGGCTGGG
                    ************************************************************

HsCD00329443        GGTGCCCAGCATGTCCGCTCGCACTCGTCGCCCGCGTCCCTGCAGCTGGGCACCGGCGCG
NM_001168278.1      GGTGCCCAGCATGTCCGCTCGCACTCGTCGCCCGCGTCCCTGCAGCTGGGCACCGGCGCG
NM_015472.4         GGTGCCCAGCATGTCCGCTCGCACTCGTCGCCCGCGTCCCTGCAGCTGGGCACCGGCGCG
NM_001168280.1      GGTGCCCAGCATGTCCGCTCGCACTCGTCGCCCGCGTCCCTGCAGCTGGGCACCGGCGCG
                    ************************************************************

HsCD00329443        GGTGCTGCGGGTAGCCCCGCGCAGCAGCACGCGCACCTCCGCCAGCAGTCCTACGACGTG
NM_001168278.1      GGTGCTGCGGGTAGCCCCGCGCAGCAGCACGCGCACCTCCGCCAGCAGTCCTACGACGTG
NM_015472.4         GGTGCTGCGGGTAGCCCCGCGCAGCAGCACGCGCACCTCCGCCAGCAGTCCTACGACGTG
NM_001168280.1      GGTGCTGCGGGTAGCCCCGCGCAGCAGCACGCGCACCTCCGCCAGCAGTCCTACGACGTG
                    ************************************************************

HsCD00329443        ACCGACGAGCTGCCACTGCCCCCGGGCTGGGAGATGACCTTCACGGCCACTGGCCAGAGG
NM_001168278.1      ACCGACGAGCTGCCACTGCCCCCGGGCTGGGAGATGACCTTCACGGCCACTGGCCAGAGG
NM_015472.4         ACCGACGAGCTGCCACTGCCCCCGGGCTGGGAGATGACCTTCACGGCCACTGGCCAGAGG
NM_001168280.1      ACCGACGAGCTGCCACTGCCCCCGGGCTGGGAGATGACCTTCACGGCCACTGGCCAGAGG
                    ************************************************************

HsCD00329443        TACTTCCTCAATCACATAGAAAAAATCACCACATGGCAAGACCCTAGGAAGGCGATGAAT
NM_001168278.1      TACTTCCTCAATCACATAGAAAAAATCACCACATGGCAAGACCCTAGGAAGGCGATGAAT
NM_015472.4         TACTTCCTCAATCACATAGAAAAAATCACCACATGGCAAGACCCTAGGAAGGCGATGAAT
NM_001168280.1      TACTTCCTCAATCACATAGAAAAAATCACCACATGGCAAGACCCTAGGAAGGCGATGAAT
                    ************************************************************

HsCD00329443        CAGCCTCTGAATCATATGAACCTCCACCCTGCCGTCAGTTCCACACCAGTGCCTCAGAGG
NM_001168278.1      CAGCCTCTGAATCATATGAACCTCCACCCTGCCGTCAGTTCCACACCAGTGCCTCAGAGG
NM_015472.4         CAGCCTCTGAATCATATGAACCTCCACCCTGCCGTCAGTTCCACACCAGTGCCTCAGAGG
NM_001168280.1      CAGCCTCTGAATCATATGAACCTCCACCCTGCCGTCAGTTCCACACCAGTGCCTCAGAGG
                    ************************************************************

HsCD00329443        TCCATGGCAGTATCCCAGCCAAATCTCGTGATGAATCACCAACACCAGCAGCAGATGGCC
NM_001168278.1      TCCATGGCAGTATCCCAGCCAAATCTCGTGATGAATCACCAACACCAGCAGCAGATGGCC
NM_015472.4         TCCATGGCAGTATCCCAGCCAAATCTCGTGATGAATCACCAACACCAGCAGCAGATGGCC
NM_001168280.1      TCCATGGCAGTATCCCAGCCAAATCTCGTGATGAATCACCAACACCAGCAGCAGATGGCC
                    ************************************************************

HsCD00329443        CCCAGTACCCTGAGCCAGCAGAACCACCCCACTCAGAACCCACCCGCAGGGCTCATGAGT
NM_001168278.1      CCCAGTACCCTGAGCCAGCAGAACCACCCCACTCAGAACCCACCCGCAGGGCTCATGAGT
NM_015472.4         CCCAGTACCCTGAGCCAGCAGAACCACCCCACTCAGAACCCACCCGCAGGGCTCATGAGT
NM_001168280.1      CCCAGTACCCTGAGCCAGCAGAACCACCCCACTCAGAACCCACCCGCAGGGCTCATGAGT
                    ************************************************************

HsCD00329443        ATGCCCAATGCGCTGACCACTCAGCAGCAGCAGCAGCAGAAACTGCGGCTTCAGAGAATC
NM_001168278.1      ATGCCCAATGCGCTGACCACTCAGCAGCAGCAGCAGCAGAAACTGCGGCTTCAGAGAATC
NM_015472.4         ATGCCCAATGCGCTGACCACTCAGCAGCAGCAGCAGCAGAAACTGCGGCTTCAGAGAATC
NM_001168280.1      ATGCCCAATGCGCTGACCACTCAGCAGCAGCAGCAGCAGAAACTGCGGCTTCAGAGAATC
                    ************************************************************

HsCD00329443        CAGATGGAGAGAGAAAGGATTCGAATGCGCCAAGAGGAGCTCATGAGGCAGGAAGCTGCC
NM_001168278.1      CAGATGGAGAGAGAAAGGATTCGAATGCGCCAAGAGGAGCTCATGAGGCAGGAAGCTGCC
NM_015472.4         CAGATGGAGAGAGAAAGGATTCGAATGCGCCAAGAGGAGCTCATGAGGCAGGAAGCTGCC
NM_001168280.1      CAGATGGAGAGAGAAAGGATTCGAATGCGCCAAGAGGAGCTCATGAGGCAGGAAGCTGCC
                    ************************************************************

HsCD00329443        CTCTGTCGACAGCTCCCCATGGAAGCTGAGACTCTTGCCCCAGTTCAGGCTGCTGTCAAC
NM_001168278.1      CTCTGTCGACAGCTCCCCATGGAAGCTGAGACTCTTGCCCCAGTTCAGGCTGCTGTCAAC
NM_015472.4         CTCTGTCGACAGCTCCCCATGGAAGCTGAGACTCTTGCCCCAGTTCAGGCTGCTGTCAAC
NM_001168280.1      CTCTGTCGACAGCTCCCCATGGAAGCTGAGACTCTTGCCCCAGTTCAGGCTGCTGTCAAC
                    ************************************************************

HsCD00329443        CCACCCACGATGACCCCAGACATGAGATCCATCACTAATAATAGCTCAGATCCTTTCCTC
NM_001168278.1      CCACCCACGATGACCCCAGACATGAGATCCATCACTAATAATAGCTCAGATCCTTTCCTC
NM_015472.4         CCACCCACGATGACCCCAGACATGAGATCCATCACTAATAATAGCTCAGATCCTTTCCTC
NM_001168280.1      CCACCCACGATGACCCCAGACATGAGATCCATCACTAATAATAGCTCAGATCCTTTCCTC
                    ************************************************************

HsCD00329443        AATGGAGGGCCATATCATTCGAGGGAGCAGAGCACTGACAGTGGCCTGGGGTTAGGGTGC
NM_001168278.1      AATGGAGGGCCATATCATTCGAGGGAGCAGAGCACTGACAGTGGCCTGGGGTTAGGGTGC
NM_015472.4         AATGGAGGGCCATATCATTCGAGGGAGCAGAGCACTGACAGTGGCCTGGGGTTAGGGTGC
NM_001168280.1      AATGGAGGGCCATATCATTCGAGGGAGCAGAGCACTGACAGTGGCCTGGGGTTAGGGTGC
                    ************************************************************

HsCD00329443        TACAGTGTCCCCACAACTCCGGAGGACTTCCTCAGCAATGTGGATGAGATGGATACAGGA
NM_001168278.1      TACAGTGTCCCCACAACTCCGGAGGACTTCCTCAGCAATGTGGATGAGATGGATACAGGA
NM_015472.4         TACAGTGTCCCCACAACTCCGGAGGACTTCCTCAGCAATGTGGATGAGATGGATACAGGA
NM_001168280.1      TACAGTGTCCCCACAACTCCGGAGGACTTCCTCAGCAATGTGGATGAGATGGATACAGGA
                    ************************************************************

HsCD00329443        GAAAACGCAGGACAAACACCCATGAACATCAATCCCCAACAGACCCGTTTCCCTGATTTC
NM_001168278.1      GAAAACGCAGGACAAACACCCATGAACATCAATCCCCAACAGACCCGTTTCCCTGATTTC
NM_015472.4         GAAAACGCAGGACAAACACCCATGAACATCAATCCCCAACAGACCCGTTTCCCTGATTTC
NM_001168280.1      GAAAACGCAGGACAAACACCCATGAACATCAATCCCCAACAGACCCGTTTCCCTGATTTC
                    ************************************************************

HsCD00329443        CTTGACTGTCTTCCAGGAACAAACGTTGACTTAGGAACTTTGGAATCTGAAGACCTGATC
NM_001168278.1      CTTGACTGTCTTCCAGGAACAAACGTTGACTTAGGAACTTTGGAATCTGAAGACCTGATC
NM_015472.4         CTTGACTGTCTTCCAGGAACAAACGTTGACTTAGGAACTTTGGAATCTGAAGACCTGATC
NM_001168280.1      CTTGACTGTCTTCCAGGAACAAACGTTGACTTAGGAACTTTGGAATCTGAAGACCTGATC
                    ************************************************************

HsCD00329443        CCCCTCTTCAATGATGTAGAGTCTGCTCTGAACAAAAGTGAGCCCTTTCTAACCTGGCTG
NM_001168278.1      CCCCTCTTCAATGATGTAGAGTCTGCTCTGAACAAAAGTGAGCCCTTTCTAACCTGGCTG
NM_015472.4         CCCCTCTTCAATGATGTAGAGTCTGCTCTGAACAAAAGTGAGCCCTTTCTAACCTGGCTG
NM_001168280.1      CCCCTCTTCAATGATGTAGAGTCTGCTCTGAACAAAAGTGAGCCCTTTCTAACCTGGCTG
                    ************************************************************

HsCD00329443        TTG
NM_001168278.1      TAA
NM_015472.4         TAA
NM_001168280.1      TAA
                    *:.

Pathways

Reactome : Disease
Reactome : Fatty acid, triacylglycerol, and ketone body metabolism
Reactome : Gene Expression
Reactome : Generic Transcription Pathway
Reactome : Loss of Function of SMAD2/3 in Cancer
Reactome : Loss of Function of SMAD4 in Cancer
Reactome : Loss of Function of TGFBR1 in Cancer
Reactome : Loss of Function of TGFBR2 in Cancer
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : PPARA activates gene expression
Reactome : Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)
Reactome : SMAD2/3 MH2 Domain Mutants in Cancer
Reactome : SMAD2/3 Phosphorylation Motif Mutants in Cancer
Reactome : SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription
Reactome : SMAD4 MH2 Domain Mutants in Cancer
Reactome : Signal Transduction
Reactome : Signaling by Hippo
Reactome : Signaling by TGF-beta Receptor Complex
Reactome : Signaling by TGF-beta Receptor Complex in Cancer
Reactome : TGFBR1 KD Mutants in Cancer
Reactome : TGFBR1 LBD Mutants in Cancer
Reactome : TGFBR2 Kinase Domain Mutants in Cancer
Reactome : TGFBR2 MSI Frameshift Mutants in Cancer
Reactome : Transcriptional activity of SMAD2/SMAD3:SMAD4 heterotrimer
Reactome : YAP1- and WWTR1 (TAZ)-stimulated gene expression
WikiPathway : Adipogenesis

Protein

SMART domain : WW : Domain with 2 conserved Trp (W) residues
UniProt : Q9GZV5
HPRD : 09580

Clone

Cloning Information : 269178
HIP Master Clone ID : 174066
Original Clone ID : FLH269178.01L

Organism


TAX_ID : 9606
Species Specific ID: 25937

Genome

Chromosome : 3
Map Location : 3q23-q24
Ensembl : ENSG00000018408

Reagents

Labome : WWTR1-antibody