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RBL1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RBL1
Gene Name:  retinoblastoma-like 1 (p107)
Sequence             
Original Clone ID: FLH269187.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329452

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 3045nts         Open reading frame : 1 to 3045
Reference Sequence Alignment

ATGTTCGAGGACAAGCCCCACGCTGAGGGGGCGGCGGTGGTCGCCGCAGCCGGGGAGGCG
CTACAGGCCCTGTGCCAGGAGCTGAACCTGGACGAGGGGAGCGCGGCCGAAGCCCTGGAC
GACTTTACTGCCATCCGAGGCAACTACAGCCTAGAGGGAGAAGTTACACACTGGTTGGCA
TGTTCATTATATGTTGCATGCCGCAAAAGCATTATTCCCACGGTTGGAAAGGGTATCATG
GAAGGCAACTGTGTTTCACTTACCAGAATACTACGTTCAGCTAAATTAAGTTTAATACAA
TTTTTTAGTAAAATGAAGAAATGGATGGACATGTCAAATCTACCACAAGAATTTCGTGAA
CGTATAGAAAGGCTAGAGAGAAATTTTGAGGTGTCTACTGTAATATTCAAAAAATATGAG
CCAATTTTTTTAGATATATTTCAAAATCCATATGAAGAACCACCAAAGTTACCACGAAGC
CGGAAGCAGAGGAGGATTCCTTGCAGTGTTAAGGATCTGTTTAATTTCTGTTGGACACTT
TTTGTTTATACTAAGGGTAATTTTCGGATGATTGGGGATGACTTAGTAAACTCTTATCAT
TTACTTCTATGCTGCTTGGATCTGATTTTTGCCAATGCGATTATGTGCCCAAATAGACAA
GACTTGCTAAATCCATCATTTAAAGGTTTACCATCTGATTTTCATACTGCTGACTTTACG
GCTTCTGAAGAGCCACCCTGCATCATTGCTGTACTGTGTGAACTGCATGATGGACTTCTC
GTAGAAGCAAAAGGAATAAAGGAGCACTACTTTAAGCCATATATTTCAAAACTCTTTGAC
AGGAAGATATTAAAAGGAGAATGCCTCCTGGACCTTTCAAGTTTTACTGATAATAGCAAA
GCAGTGAATAAGGAGTATGAAGAGTATGTTCTAACTGTTGGTGATTTTGATGAGAGGATC
TTTTTGGGAGCAGACGCAGAAGAGGAAATTGGAACACCTCGAAAGTTCACTCGTGACACC
CCATTAGGGAAACTGACAGCACAGGCTAATGTGGAGTATAACCTTCAACAGCACTTTGAA
AAAAAAAGGTCATTTGCACCTTCTACCCCACTGACCGGACGGAGATATTTACGAGAAAAA
GAAGCAGTCATTACTCCTGTTGCATCAGCCACCCAAAGTGTGAGCCGGTTACAGAGTATT
GTGGCTGGTCTGAAAAATGCACCAAGTGACCAACTTATAAATATTTTTGAATCTTGTGTG
CGTAATCCTGTGGAAAACATTATGAAAATACTAAAAGGAATAGGAGAGACTTTCTGTCAA
CACTATACTCAATCAACAGATGAACAGCCAGGATCTCACATAGACTTTGCTGTAAACAGA
CTAAAGCTGGCAGAAATTTTGTATTATAAAATACTAGAGACTGTAATGGTTCAGGAAACA
CGAAGACTTCATGGAATGGACATGTCAGTTCTTTTAGAGCAAGATATATTTCATCGTTCC
TTGATGGCTTGTTGTTTGGAAATTGTGCTCTTTGCCTATAGCTCACCTCGTACTTTTCCT
TGGATTATTGAAGTTCTCAACTTGCAACCATTTTACTTTTATAAGGTTATTGAGGTGGTG
ATCCGCTCAGAAGAGGGGCTCTCAAGGGACATGGTGAAACACCTAAACAGCATTGAAGAA
CAGATTTTGGAGAGTTTAGCATGGAGTCACGATTCTGCACTGTGGGAGGCTCTCCAGGTT
TCTGCAAACAAAGTTCCTACCTGTGAAGAAGTTATATTCCCAAATAACTTTGAAACAGGA
AATGGAGGAAATGTGCAGGGACATCTTCCCCTGATGCCAATGTCTCCTCTAATGCACCCA
AGAGTCAAGGAAGTTCGAACTGACAGTGGGAGTCTTCGAAGAGATATGCAACCATTGTCT
CCAATTTCTGTCCATGAACGCTACAGTTCTCCTACCGCAGGGAGTGCTAAGAGAAGACTC
TTTGGAGAGGACCCCCCAAAGGAAATGCTTATGGACAAGATCATAACAGAAGGAACAAAA
TTGAAAATCGCTCCTTCTTCAAGCATTACTGCTGAAAATGTATCAATTTTACCTGGTCAA
ACTCTTCTAACAATGGCCACAGCCCCAGTAACAGGAACAACAGGACATAAAGTTACAATT
CCATTACATGGTGTCGCAAATGATGCTGGAGAGATCACACTGATACCTCTTTCCATGAAT
ACAAATCAGGAGTCCAAAGTCAAGAGTCCTGTATCACTTACTGCTCATTCATTAATTGGT
GCTTCTCCAAAACAGACCAATCTGACTAAAGCACAAGAGGTACATTCAACTGGAATAAAC
AGGCCAAAGAGAACTGGGTCCTTAGCACTATTTTACAGAAAGGTCTATCATTTGGCAAGT
GTACGCTTACGTGATCTATGTCTAAAACTGGATGTTTCAAATGAGTTACGAAGGAAGATA
TGGACGTGTTTTGAATTCACTTTAGTTCACTGTCCTGATCTAATGAAAGACAGGCATTTG
GATCAGCTCCTCCTTTGTGCCTTTTATATCATGGCAAAGGTAACAAAAGAAGAAAGAACT
TTTCAAGAAATTATGAAAAGTTATAGGAATCAGCCCCAAGCTAATAGTCACGTATATAGA
AGTGTTCTGCTGAAAAGTATTCCAAGAGAAGTTGTGGCATATAATAAAAATATAAATGAT
GACTTTGAAATGATAGATTGTGACTTAGAAGATGCTACAAAAACACCTGACTGTTCCAGT
GGACCAGTGAAAGAGGAAAGAGGTGATCTTATAAAATTTTACAATACAATATATGTAGGA
AGAGTGAAGTCATTTGCACTGAAATACGACTTGGCGAATCAGGACCATATGATGGATGCT
CCACCACTCTCTCCTTTTCCACATATTAAACAACAGCCAGGCTCACCACGCCGCATTTCC
CAGCAGCACTCCATTTATATTTCCCCGCACAAGAATGGGTCAGGCCTTACACCAAGAAGC
GCTCTGCTGTACAAGTTCAATGGCAGCCCTTCTAAGGTAAGGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 3115
Start on reference sequence 71
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RBL1
Symbol Nomenclature : RBL1
SYNONYM : CP107; PRB1; p107
Designation : 107 kDa retinoblastoma-associated protein|cellular protein 107|retinoblastoma-like protein 1
Full Nomenclature : retinoblastoma-like 1 (p107)
GENEID : 5933
Locus Tag : RP11-382A12.1
HGNC : 9893
MIM : 116957
Vega : OTTHUMG00000032406
Target GenBank: BC032247

 

Reference Sequence Alignment

  

HsCD00329452      ATGTTCGAGGACAAGCCCCACGCTGAGGGGGCGGCGGTGGTCGCCGCAGCCGGGGAGGCG
NM_002895.3       ATGTTCGAGGACAAGCCCCACGCTGAGGGGGCGGCGGTGGTCGCCGCAGCCGGGGAGGCG
NM_183404.2       ATGTTCGAGGACAAGCCCCACGCTGAGGGGGCGGCGGTGGTCGCCGCAGCCGGGGAGGCG
                  ************************************************************

HsCD00329452      CTACAGGCCCTGTGCCAGGAGCTGAACCTGGACGAGGGGAGCGCGGCCGAAGCCCTGGAC
NM_002895.3       CTACAGGCCCTGTGCCAGGAGCTGAACCTGGACGAGGGGAGCGCGGCCGAAGCCCTGGAC
NM_183404.2       CTACAGGCCCTGTGCCAGGAGCTGAACCTGGACGAGGGGAGCGCGGCCGAAGCCCTGGAC
                  ************************************************************

HsCD00329452      GACTTTACTGCCATCCGAGGCAACTACAGCCTAGAGGGAGAAGTTACACACTGGTTGGCA
NM_002895.3       GACTTTACTGCCATCCGAGGCAACTACAGCCTAGAGGGAGAAGTTACACACTGGTTGGCA
NM_183404.2       GACTTTACTGCCATCCGAGGCAACTACAGCCTAGAGGGAGAAGTTACACACTGGTTGGCA
                  ************************************************************

HsCD00329452      TGTTCATTATATGTTGCATGCCGCAAAAGCATTATTCCCACGGTTGGAAAGGGTATCATG
NM_002895.3       TGTTCATTATATGTTGCATGCCGCAAAAGCATTATTCCCACGGTTGGAAAGGGTATCATG
NM_183404.2       TGTTCATTATATGTTGCATGCCGCAAAAGCATTATTCCCACGGTTGGAAAGGGTATCATG
                  ************************************************************

HsCD00329452      GAAGGCAACTGTGTTTCACTTACCAGAATACTACGTTCAGCTAAATTAAGTTTAATACAA
NM_002895.3       GAAGGCAACTGTGTTTCACTTACCAGAATACTACGTTCAGCTAAATTAAGTTTAATACAA
NM_183404.2       GAAGGCAACTGTGTTTCACTTACCAGAATACTACGTTCAGCTAAATTAAGTTTAATACAA
                  ************************************************************

HsCD00329452      TTTTTTAGTAAAATGAAGAAATGGATGGACATGTCAAATCTACCACAAGAATTTCGTGAA
NM_002895.3       TTTTTTAGTAAAATGAAGAAATGGATGGACATGTCAAATCTACCACAAGAATTTCGTGAA
NM_183404.2       TTTTTTAGTAAAATGAAGAAATGGATGGACATGTCAAATCTACCACAAGAATTTCGTGAA
                  ************************************************************

HsCD00329452      CGTATAGAAAGGCTAGAGAGAAATTTTGAGGTGTCTACTGTAATATTCAAAAAATATGAG
NM_002895.3       CGTATAGAAAGGCTAGAGAGAAATTTTGAGGTGTCTACTGTAATATTCAAAAAATATGAG
NM_183404.2       CGTATAGAAAGGCTAGAGAGAAATTTTGAGGTGTCTACTGTAATATTCAAAAAATATGAG
                  ************************************************************

HsCD00329452      CCAATTTTTTTAGATATATTTCAAAATCCATATGAAGAACCACCAAAGTTACCACGAAGC
NM_002895.3       CCAATTTTTTTAGATATATTTCAAAATCCATATGAAGAACCACCAAAGTTACCACGAAGC
NM_183404.2       CCAATTTTTTTAGATATATTTCAAAATCCATATGAAGAACCACCAAAGTTACCACGAAGC
                  ************************************************************

HsCD00329452      CGGAAGCAGAGGAGGATTCCTTGCAGTGTTAAGGATCTGTTTAATTTCTGTTGGACACTT
NM_002895.3       CGGAAGCAGAGGAGGATTCCTTGCAGTGTTAAGGATCTGTTTAATTTCTGTTGGACACTT
NM_183404.2       CGGAAGCAGAGGAGGATTCCTTGCAGTGTTAAGGATCTGTTTAATTTCTGTTGGACACTT
                  ************************************************************

HsCD00329452      TTTGTTTATACTAAGGGTAATTTTCGGATGATTGGGGATGACTTAGTAAACTCTTATCAT
NM_002895.3       TTTGTTTATACTAAGGGTAATTTTCGGATGATTGGGGATGACTTAGTAAACTCTTATCAT
NM_183404.2       TTTGTTTATACTAAGGGTAATTTTCGGATGATTGGGGATGACTTAGTAAACTCTTATCAT
                  ************************************************************

HsCD00329452      TTACTTCTATGCTGCTTGGATCTGATTTTTGCCAATGCGATTATGTGCCCAAATAGACAA
NM_002895.3       TTACTTCTATGCTGCTTGGATCTGATTTTTGCCAATGCGATTATGTGCCCAAATAGACAA
NM_183404.2       TTACTTCTATGCTGCTTGGATCTGATTTTTGCCAATGCGATTATGTGCCCAAATAGACAA
                  ************************************************************

HsCD00329452      GACTTGCTAAATCCATCATTTAAAGGTTTACCATCTGATTTTCATACTGCTGACTTTACG
NM_002895.3       GACTTGCTAAATCCATCATTTAAAGGTTTACCATCTGATTTTCATACTGCTGACTTTACG
NM_183404.2       GACTTGCTAAATCCATCATTTAAAGGTTTACCATCTGATTTTCATACTGCTGACTTTACG
                  ************************************************************

HsCD00329452      GCTTCTGAAGAGCCACCCTGCATCATTGCTGTACTGTGTGAACTGCATGATGGACTTCTC
NM_002895.3       GCTTCTGAAGAGCCACCCTGCATCATTGCTGTACTGTGTGAACTGCATGATGGACTTCTC
NM_183404.2       GCTTCTGAAGAGCCACCCTGCATCATTGCTGTACTGTGTGAACTGCATGATGGACTTCTC
                  ************************************************************

HsCD00329452      GTAGAAGCAAAAGGAATAAAGGAGCACTACTTTAAGCCATATATTTCAAAACTCTTTGAC
NM_002895.3       GTAGAAGCAAAAGGAATAAAGGAGCACTACTTTAAGCCATATATTTCAAAACTCTTTGAC
NM_183404.2       GTAGAAGCAAAAGGAATAAAGGAGCACTACTTTAAGCCATATATTTCAAAACTCTTTGAC
                  ************************************************************

HsCD00329452      AGGAAGATATTAAAAGGAGAATGCCTCCTGGACCTTTCAAGTTTTACTGATAATAGCAAA
NM_002895.3       AGGAAGATATTAAAAGGAGAATGCCTCCTGGACCTTTCAAGTTTTACTGATAATAGCAAA
NM_183404.2       AGGAAGATATTAAAAGGAGAATGCCTCCTGGACCTTTCAAGTTTTACTGATAATAGCAAA
                  ************************************************************

HsCD00329452      GCAGTGAATAAGGAGTATGAAGAGTATGTTCTAACTGTTGGTGATTTTGATGAGAGGATC
NM_002895.3       GCAGTGAATAAGGAGTATGAAGAGTATGTTCTAACTGTTGGTGATTTTGATGAGAGGATC
NM_183404.2       GCAGTGAATAAGGAGTATGAAGAGTATGTTCTAACTGTTGGTGATTTTGATGAGAGGATC
                  ************************************************************

HsCD00329452      TTTTTGGGAGCAGACGCAGAAGAGGAAATTGGAACACCTCGAAAGTTCACTCGTGACACC
NM_002895.3       TTTTTGGGAGCAGACGCAGAAGAGGAAATTGGAACACCTCGAAAGTTCACTCGTGACACC
NM_183404.2       TTTTTGGGAGCAGACGCAGAAGAGGAAATTGGAACACCTCGAAAGTTCACTCGTGACACC
                  ************************************************************

HsCD00329452      CCATTAGGGAAACTGACAGCACAGGCTAATGTGGAGTATAACCTTCAACAGCACTTTGAA
NM_002895.3       CCATTAGGGAAACTGACAGCACAGGCTAATGTGGAGTATAACCTTCAACAGCACTTTGAA
NM_183404.2       CCATTAGGGAAACTGACAGCACAGGCTAATGTGGAGTATAACCTTCAACAGCACTTTGAA
                  ************************************************************

HsCD00329452      AAAAAAAGGTCATTTGCACCTTCTACCCCACTGACCGGACGGAGATATTTACGAGAAAAA
NM_002895.3       AAAAAAAGGTCATTTGCACCTTCTACCCCACTGACCGGACGGAGATATTTACGAGAAAAA
NM_183404.2       AAAAAAAGGTCATTTGCACCTTCTACCCCACTGACCGGACGGAGATATTTACGAGAAAAA
                  ************************************************************

HsCD00329452      GAAGCAGTCATTACTCCTGTTGCATCAGCCACCCAAAGTGTGAGCCGGTTACAGAGTATT
NM_002895.3       GAAGCAGTCATTACTCCTGTTGCATCAGCCACCCAAAGTGTGAGCCGGTTACAGAGTATT
NM_183404.2       GAAGCAGTCATTACTCCTGTTGCATCAGCCACCCAAAGTGTGAGCCGGTTACAGAGTATT
                  ************************************************************

HsCD00329452      GTGGCTGGTCTGAAAAATGCACCAAGTGACCAACTTATAAATATTTTTGAATCTTGTGTG
NM_002895.3       GTGGCTGGTCTGAAAAATGCACCAAGTGACCAACTTATAAATATTTTTGAATCTTGTGTG
NM_183404.2       GTGGCTGGTCTGAAAAATGCACCAAGTGACCAACTTATAAATATTTTTGAATCTTGTGTG
                  ************************************************************

HsCD00329452      CGTAATCCTGTGGAAAACATTATGAAAATACTAAAAGGAATAGGAGAGACTTTCTGTCAA
NM_002895.3       CGTAATCCTGTGGAAAACATTATGAAAATACTAAAAGGAATAGGAGAGACTTTCTGTCAA
NM_183404.2       CGTAATCCTGTGGAAAACATTATGAAAATACTAAAAGGAATAGGAGAGACTTTCTGTCAA
                  ************************************************************

HsCD00329452      CACTATACTCAATCAACAGATGAACAGCCAGGATCTCACATAGACTTTGCTGTAAACAGA
NM_002895.3       CACTATACTCAATCAACAGATGAACAGCCAGGATCTCACATAGACTTTGCTGTAAACAGA
NM_183404.2       CACTATACTCAATCAACAGATGAACAGCCAGGATCTCACATAGACTTTGCTGTAAACAGA
                  ************************************************************

HsCD00329452      CTAAAGCTGGCAGAAATTTTGTATTATAAAATACTAGAGACTGTAATGGTTCAGGAAACA
NM_002895.3       CTAAAGCTGGCAGAAATTTTGTATTATAAAATACTAGAGACTGTAATGGTTCAGGAAACA
NM_183404.2       CTAAAGCTGGCAGAAATTTTGTATTATAAAATACTAGAGACTGTAATGGTTCAGGAAACA
                  ************************************************************

HsCD00329452      CGAAGACTTCATGGAATGGACATGTCAGTTCTTTTAGAGCAAGATATATTTCATCGTTCC
NM_002895.3       CGAAGACTTCATGGAATGGACATGTCAGTTCTTTTAGAGCAAGATATATTTCATCGTTCC
NM_183404.2       CGAAGACTTCATGGAATGGACATGTCAGTTCTTTTAGAGCAAGATATATTTCATCGTTCC
                  ************************************************************

HsCD00329452      TTGATGGCTTGTTGTTTGGAAATTGTGCTCTTTGCCTATAGCTCACCTCGTACTTTTCCT
NM_002895.3       TTGATGGCTTGTTGTTTGGAAATTGTGCTCTTTGCCTATAGCTCACCTCGTACTTTTCCT
NM_183404.2       TTGATGGCTTGTTGTTTGGAAATTGTGCTCTTTGCCTATAGCTCACCTCGTACTTTTCCT
                  ************************************************************

HsCD00329452      TGGATTATTGAAGTTCTCAACTTGCAACCATTTTACTTTTATAAGGTTATTGAGGTGGTG
NM_002895.3       TGGATTATTGAAGTTCTCAACTTGCAACCATTTTACTTTTATAAGGTTATTGAGGTGGTG
NM_183404.2       TGGATTATTGAAGTTCTCAACTTGCAACCATTTTACTTTTATAAGGTTATTGAGGTGGTG
                  ************************************************************

HsCD00329452      ATCCGCTCAGAAGAGGGGCTCTCAAGGGACATGGTGAAACACCTAAACAGCATTGAAGAA
NM_002895.3       ATCCGCTCAGAAGAGGGGCTCTCAAGGGACATGGTGAAACACCTAAACAGCATTGAAGAA
NM_183404.2       ATCCGCTCAGAAGAGGGGCTCTCAAGGGACATGGTGAAACACCTAAACAGCATTGAAGAA
                  ************************************************************

HsCD00329452      CAGATTTTGGAGAGTTTAGCATGGAGTCACGATTCTGCACTGTGGGAGGCTCTCCAGGTT
NM_002895.3       CAGATTTTGGAGAGTTTAGCATGGAGTCACGATTCTGCACTGTGGGAGGCTCTCCAGGTT
NM_183404.2       CAGATTTTGGAGAGTTTAGCATGGAGTCACGATTCTGCACTGTGGGAGGCTCTCCAGGTT
                  ************************************************************

HsCD00329452      TCTGCAAACAAAGTTCCTACCTGTGAAGAAGTTATATTCCCAAATAACTTTGAAACAGGA
NM_002895.3       TCTGCAAACAAAGTTCCTACCTGTGAAGAAGTTATATTCCCAAATAACTTTGAAACAGGA
NM_183404.2       TCTGCAAACAAAGTTCCTACCTGTGAAGAAGTTATATTCCCAAATAACTTTGAAACAGGA
                  ************************************************************

HsCD00329452      AATGGAGGAAATGTGCAGGGACATCTTCCCCTGATGCCAATGTCTCCTCTAATGCACCCA
NM_002895.3       AATGGAGGAAATGTGCAGGGACATCTTCCCCTGATGCCAATGTCTCCTCTAATGCACCCA
NM_183404.2       AATGGAGGAAATGTGCAGGGACATCTTCCCCTGATGCCAATGTCTCCTCTAATGCACCCA
                  ************************************************************

HsCD00329452      AGAGTCAAGGAAGTTCGAACTGACAGTGGGAGTCTTCGAAGAGATATGCAACCATTGTCT
NM_002895.3       AGAGTCAAGGAAGTTCGAACTGACAGTGGGAGTCTTCGAAGAGATATGCAACCATTGTCT
NM_183404.2       AGAGTCAAGGAAGTTCGAACTGACAGTGGGAGTCTTCGAAGAGATATGCAACCATTGTCT
                  ************************************************************

HsCD00329452      CCAATTTCTGTCCATGAACGCTACAGTTCTCCTACCGCAGGGAGTGCTAAGAGAAGACTC
NM_002895.3       CCAATTTCTGTCCATGAACGCTACAGTTCTCCTACCGCAGGGAGTGCTAAGAGAAGACTC
NM_183404.2       CCAATTTCTGTCCATGAACGCTACAGTTCTCCTACCGCAGGGAGTGCTAAGAGAAGACTC
                  ************************************************************

HsCD00329452      TTTGGAGAGGACCCCCCAAAGGAAATGCTTATGGACAAGATCATAACAGAAGGAACAAAA
NM_002895.3       TTTGGAGAGGACCCCCCAAAGGAAATGCTTATGGACAAGATCATAACAGAAGGAACAAAA
NM_183404.2       TTTGGAGAGGACCCCCCAAAGGAAATGCTTATGGACAAGATCATAACAGAAGGAACAAAA
                  ************************************************************

HsCD00329452      TTGAAAATCGCTCCTTCTTCAAGCATTACTGCTGAAAATGTATCAATTTTACCTGGTCAA
NM_002895.3       TTGAAAATCGCTCCTTCTTCAAGCATTACTGCTGAAAATGTATCAATTTTACCTGGTCAA
NM_183404.2       TTGAAAATCGCTCCTTCTTCAAGCATTACTGCTGAAAATGTATCAATTTTACCTGGTCAA
                  ************************************************************

HsCD00329452      ACTCTTCTAACAATGGCCACAGCCCCAGTAACAGGAACAACAGGACATAAAGTTACAATT
NM_002895.3       ACTCTTCTAACAATGGCCACAGCCCCAGTAACAGGAACAACAGGACATAAAGTTACAATT
NM_183404.2       ACTCTTCTAACAATGGCCACAGCCCCAGTAACAGGAACAACAGGACATAAAGTTACAATT
                  ************************************************************

HsCD00329452      CCATTACATGGTGTCGCAAATGATGCTGGAGAGATCACACTGATACCTCTTTCCATGAAT
NM_002895.3       CCATTACATGGTGTCGCAAATGATGCTGGAGAGATCACACTGATACCTCTTTCCATGAAT
NM_183404.2       CCATTACATGGTGTCGCAAATGATGCTGGAGAGATCACACTGATACCTCTTTCCATGAAT
                  ************************************************************

HsCD00329452      ACAAATCAGGAGTCCAAAGTCAAGAGTCCTGTATCACTTACTGCTCATTCATTAATTGGT
NM_002895.3       ACAAATCAGGAGTCCAAAGTCAAGAGTCCTGTATCACTTACTGCTCATTCATTAATTGGT
NM_183404.2       ACAAATCAGGAGTCCAAAGTCAAGAGTCCTGTATCACTTACTGCTCATTCATTAATTGGT
                  ************************************************************

HsCD00329452      GCTTCTCCAAAACAGACCAATCTGACTAAAGCACAAGAGGTACATTCAACTGGAATAAAC
NM_002895.3       GCTTCTCCAAAACAGACCAATCTGACTAAAGCACAAGAGGTACATTCAACTGGAATAAAC
NM_183404.2       GCTTCTCCAAAACAGACCAATCTGACTAAAGCACAAGAGGTACATTCAACTGGAATAAAC
                  ************************************************************

HsCD00329452      AGGCCAAAGAGAACTGGGTCCTTAGCACTATTTTACAGAAAGGTCTATCATTTGGCAAGT
NM_002895.3       AGGCCAAAGAGAACTGGGTCCTTAGCACTATTTTACAGAAAGGTCTATCATTTGGCAAGT
NM_183404.2       AGGCCAAAGAGAACTGGGTCCTTAGCACTATTTTACAGAAAGGTCTATCATTTGGCAAGT
                  ************************************************************

HsCD00329452      GTACGCTTACGTGATCTATGTCTAAAACTGGATGTTTCAAATGAGTTACGAAGGAAGATA
NM_002895.3       GTACGCTTACGTGATCTATGTCTAAAACTGGATGTTTCAAATGAGTTACGAAGGAAGATA
NM_183404.2       GTACGCTTACGTGATCTATGTCTAAAACTGGATGTTTCAAATGAGTTACGAAGGAAGATA
                  ************************************************************

HsCD00329452      TGGACGTGTTTTGAATTCACTTTAGTTCACTGTCCTGATCTAATGAAAGACAGGCATTTG
NM_002895.3       TGGACGTGTTTTGAATTCACTTTAGTTCACTGTCCTGATCTAATGAAAGACAGGCATTTG
NM_183404.2       TGGACGTGTTTTGAATTCACTTTAGTTCACTGTCCTGATCTAATGAAAGACAGGCATTTG
                  ************************************************************

HsCD00329452      GATCAGCTCCTCCTTTGTGCCTTTTATATCATGGCAAAGGTAACAAAAGAAGAAAGAACT
NM_002895.3       GATCAGCTCCTCCTTTGTGCCTTTTATATCATGGCAAAGGTAACAAAAGAAGAAAGAACT
NM_183404.2       GATCAGCTCCTCCTTTGTGCCTTTTATATCATGGCAAAGGTAACAAAAGAAGAAAGAACT
                  ************************************************************

HsCD00329452      TTTCAAGAAATTATGAAAAGTTATAGGAATCAGCCCCAAGCTAATAGTCACGTATATAGA
NM_002895.3       TTTCAAGAAATTATGAAAAGTTATAGGAATCAGCCCCAAGCTAATAGTCACGTATATAGA
NM_183404.2       TTTCAAGAAATTATGAAAAGTTATAGGAATCAGCCCCAAGCTAATAGTCACGTATATAGA
                  ************************************************************

HsCD00329452      AGTGTTCTGCTGAAAAGTATTCCAAGAGAAGTTGTGGCATATAATAAAAATATAAATGAT
NM_002895.3       AGTGTTCTGCTGAAAAGTATTCCAAGAGAAGTTGTGGCATATAATAAAAATATAAATGAT
NM_183404.2       AGTGTTCTGCTGAAAAGTATTCCAAGAGAAGTTGTGGCATATAATAAAAATATAAATGAT
                  ************************************************************

HsCD00329452      GACTTTGAAATGATAGATTGTGACTTAGAAGATGCTACAAAAACACCTGACTGTTCCAGT
NM_002895.3       GACTTTGAAATGATAGATTGTGACTTAGAAGATGCTACAAAAACACCTGACTGTTCCAGT
NM_183404.2       GACTTTGAAATGATAGATTGTGACTTAGAAGATGCTACAAAAACACCTGACTGTTCCAGT
                  ************************************************************

HsCD00329452      GGACCAGTGAAAGAGGAAAGAGGTGATCTTATAAAATTTTACAATACAATATATGTAGGA
NM_002895.3       GGACCAGTGAAAGAGGAAAGAGGTGATCTTATAAAATTTTACAATACAATATATGTAGGA
NM_183404.2       GGACCAGTGAAAGAGGAAAGAGGTGATCTTATAAAATTTTACAATACAATATATGTAGGA
                  ************************************************************

HsCD00329452      AGAGTGAAGTCATTTGCACTGAAATACGACTTGGCGAATCAGGACCATATGATGGATGCT
NM_002895.3       AGAGTGAAGTCATTTGCACTGAAATACGACTTGGCGAATCAGGACCATATGATGGATGCT
NM_183404.2       AGAGTGAAGTCATTTGCACTGAAATACGACTTGGCGAATCAGGACCATATGATGGATGCT
                  ************************************************************

HsCD00329452      CCACCACTCTCTCCTTTTCCACATATTAAACAACAGCCAGGCTCACCACGCCGCATTTCC
NM_002895.3       CCACCACTCTCTCCTTTTCCACATATTAAACAACAGCCAGGCTCACCACGCCGCATTTCC
NM_183404.2       CCACCACTCTCTCCTTTTCCACATATTAAACAACAGCCAGGCTCACCACGCCGCATTTCC
                  ************************************************************

HsCD00329452      CAGCAGCACTCCATTTATATTTCCCCGCACAAGAATGGGTCAGGCCTTACACCAAGAAGC
NM_002895.3       CAGCAGCACTCCATTTATATTTCCCCGCACAAGAATGGGTCAGGCCTTACACCAAGAAGC
NM_183404.2       CAGCAGCACTCCATTTATATTTCCCCGCACAAGAATGGGTCAGGCCTTACACCAAGAAGC
                  ************************************************************

HsCD00329452      GCTCTGCTGTACAAGTTCAATGGCAGCCCTTCTAAGGTAAGGTTG---------------
NM_002895.3       GCTCTGCTGTACAAGTTCAATGGCAGCCCTTCTAAGAGTTTGAAAGATATCAACAACATG
NM_183404.2       GCTCTGCTGTACAAGTTCAATGGCAGCCCTTCTAAGGTAAGGTGA---------------
                  ************************************. :: *: .               

HsCD00329452      ------------------------------------------------------------
NM_002895.3       ATAAGGCAAGGTGAGCAGAGAACCAAGAAGCGAGTAATAGCCATCGATAGTGATGCAGAA
NM_183404.2       ------------------------------------------------------------
                                                                              

HsCD00329452      ------------------------------------------------------------
NM_002895.3       TCCCCTGCCAAACGCGTCTGTCAAGAAAATGATGACGTTTTACTGAAACGACTACAGGAT
NM_183404.2       ------------------------------------------------------------
                                                                              

HsCD00329452      ---------------------------
NM_002895.3       GTTGTCAGTGAAAGAGCAAATCATTAA
NM_183404.2       ---------------------------
                                             

Pathways

NCI : E2F transcription factor network
NCI : Regulation of nuclear SMAD2/3 signaling
NCI : Validated targets of C-MYC transcriptional repression
Panther : p53 pathway feedback loops 2
Reactome : Cell Cycle
Reactome : Cell Cycle, Mitotic
Reactome : Cyclin D associated events in G1
Reactome : Disease
Reactome : G0 and Early G1
Reactome : G1 Phase
Reactome : Gene Expression
Reactome : Generic Transcription Pathway
Reactome : Loss of Function of SMAD2/3 in Cancer
Reactome : Loss of Function of SMAD4 in Cancer
Reactome : Loss of Function of TGFBR1 in Cancer
Reactome : Loss of Function of TGFBR2 in Cancer
Reactome : Mitotic G1-G1/S phases
Reactome : SMAD2/3 MH2 Domain Mutants in Cancer
Reactome : SMAD2/3 Phosphorylation Motif Mutants in Cancer
Reactome : SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription
Reactome : SMAD4 MH2 Domain Mutants in Cancer
Reactome : Signal Transduction
Reactome : Signaling by TGF-beta Receptor Complex
Reactome : Signaling by TGF-beta Receptor Complex in Cancer
Reactome : TGFBR1 KD Mutants in Cancer
Reactome : TGFBR1 LBD Mutants in Cancer
Reactome : TGFBR2 Kinase Domain Mutants in Cancer
Reactome : TGFBR2 MSI Frameshift Mutants in Cancer
Reactome : Transcriptional activity of SMAD2/SMAD3:SMAD4 heterotrimer
WikiPathway : Adipogenesis
WikiPathway : Cell cycle
WikiPathway : G1 to S cell cycle control
WikiPathway : ID signaling pathway
WikiPathway : TGF beta Signaling Pathway

Clone

Cloning Information : 269187
HIP Master Clone ID : 142632
Original Clone ID : FLH269187.01L

Organism


TAX_ID : 9606
Species Specific ID: 5933

Genome

Chromosome : 20
Map Location : 20q11.2
Ensembl : ENSG00000080839

Reagents

Labome : p107-antibody