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ZNF317 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF317
Gene Name:  zinc finger protein 317
Sequence             
Original Clone ID: FLH268348.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329461

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1788nts         Open reading frame : 1 to 1788
Reference Sequence Alignment

ATGGCAGCTCTGTCCCCCACATTTGCCACGTCCACCCAGGATTCCACCTGTCTCCAGGAC
TCAGAATTTCCTGTTTCTTCAAAAGACCATTCCTGTCCCCAGAATTTGGACCTGTTCGTG
TGCAGTGGTCTGGAGCCTCACACACCCAGTGTTGGTTCCCAGGAATCGGTGACTTTCCAA
GATGTCGCTGTGGACTTTACCGAGAAGGAGTGGCCCTTGCTGGATTCTTCTCAGAGAAAA
CTGTACAAAGATGTAATGCTGGAGAACTACAGCAACCTCACTTCACTGGGGTATCAGGTC
GGCAAACCCAGCCTCATCTCACACTTGGAGCAGGAGGAGGAGCCGAGGACAGAGGAGAGG
GGCGCTCACCAGGGCGCTTGTGCAGATTGGGAGACTCCATCTAAAACCAAGTGGTCACTT
CTTATGGAAGATATTTTTGGGAAGGAAACGCCCAGTGGTGTGACGATGGAAAGAGCCGGT
CTTGGAGAGAAGTCCACTGAATACGCTCACTTGTTCGAAGTCTTTGGCATGGACCCTCAT
CTCACTCAGCCAATGGGAAGGCACGCTGGCAAGAGGCCCTATCACCGCCGCGACTATGGG
GTAGCGTTCAAGGGCAGGCCGCACCTCACTCAGCACATGAGCATGTACGACGGGAGAAAA
ATGCATGAATGTCATCAGTGCCAAAAAGCCTTCACCACGAGCGCGTCCCTCACACGGCAC
AGGAGAATCCACACCGGGGAGAAGCCTTACGAGTGCAGCGACTGCGGGAAAGCCTTCAAC
GACCCTTCAGCCCTTAGGAGCCACGCAAGAACTCACCTCAAAGAGAAGCCCTTTGACTGC
AGTCAGTGTGGAAATGCATTCCGGACCCTCTCGGCCCTGAAAATCCACATGCGAGTTCAC
ACTGGCGAGAGGCCTTACAAGTGTGATCAGTGCGGGAAGGCTTACGGCCGGAGCTGCCAC
CTCATCGCACACAAGAGAACGCACACCGGAGAGAGGCCCTACGAGTGTCACGACTGTGGG
AAAGCTTTCCAGCACCCCTCCCACCTCAAAGAGCACGTGAGGAATCACACGGGGGAGAAG
CCCTACGCGTGCACGCAGTGCGGCAAAGCCTTCCGCTGGAAGTCCAACTTTAATTTGCAC
AAGAAGAACCACATGGTGGAGAAGACCTACGAATGTAAAGAATGCGGGAAATCCTTTGGC
GATCTCGTGTCCCGGAGGAAACACATGAGGATTCACATCGTCAAGAAACCCGTGGAATGT
CGGCAGTGCGGGAAGACCTTCCGAAACCAGTCCATCCTTAAGACTCACATGAACTCTCAC
ACTGGAGAGAAACCATACGGGTGCGATCTCTGCGGGAAAGCTTTCAGCGCGAGTTCAAAC
CTCACCGCACACAGGAAGATACACACGCAAGAGAGACGCTACGAATGCGCCGCCTGCGGG
AAAGTCTTCGGTGACTATTTATCCCGGCGGAGGCACATGAGCGTTCACCTTGTAAAGAAA
CGAGTTGAGTGTAGGCAGTGTGGCAAGGCCTTCAGGAACCAGTCAACGCTGAAGACGCAC
ATGCGAAGCCACACGGGGGAGAAACCGTACGAATGCGATCACTGTGGGAAGGCCTTCAGC
ATAGGCTCCAACCTGAATGTGCACAGGCGGATCCACACCGGGGAGAAGCCCTACGAATGC
CTTGTCTGCGGGAAAGCCTTCAGCGACCACTCATCCCTCAGGAGCCACGTGAAAACTCAC
CGGGGAGAGAAGCTCTTTGTGTCATCCGTGTGGAAAAGGCTCCAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2063
Start on reference sequence 276
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF317
Symbol Nomenclature : ZNF317
Designation : KRAB-containing zinc finger protein 317
Full Nomenclature : zinc finger protein 317
GENEID : 57693
HGNC : 13507
MIM : 613864
Vega : OTTHUMG00000179932
Target GenBank: BC078154

 

Reference Sequence Alignment

  

HsCD00329461        ATGGCAGCTCTGTCCCCCACATTTGCCACGTCCACCCAGGATTCCACCTGTCTCCAGGAC
NM_001190791.1      ATGGCAGCTCTGTCCCCCACATTTGCCACGTCCACCCAGGATTCCACCTGTCTCCAGGAC
NM_020933.4         ATGGCAGCTCTGTCCCCCACATTTGCCACGTCCACCCAGGATTCCACCTGTCTCCAGGAC
                    ************************************************************

HsCD00329461        TCAGAATTTCCTGTTTCTTCAAAAGACCATTCCTGTCCCCAGAATTTGGACCTGTTCGTG
NM_001190791.1      TCAGAATTTCCTGTTTCTTCAAAAGACCATTCCTGTCCCCAGAATTTGGACCTGTTCGTG
NM_020933.4         TCAGAATTTCCTGTTTCTTCAAAAGACCATTCCTGTCCCCAGAATTTGGACCTGTTCGTG
                    ************************************************************

HsCD00329461        TGCAGTGGTCTGGAGCCTCACACACCCAGTGTTGGTTCCCAGGAATCGGTGACTTTCCAA
NM_001190791.1      TGCAGTGGTCTGGAGCCTCACACACCCAGTGTTGGTTCCCAGGAATCGGTGACTTTCCAA
NM_020933.4         TGCAGTGGTCTGGAGCCTCACACACCCAGTGTTGGTTCCCAGGAATCGGTGACTTTCCAA
                    ************************************************************

HsCD00329461        GATGTCGCTGTGGACTTTACCGAGAAGGAGTGGCCCTTGCTGGATTCTTCTCAGAGAAAA
NM_001190791.1      GATGTCGCTGTGGACTTTACCGAGAAGGAGTGGCCCTTGCTGGATTCTTCTCAGAGAAAA
NM_020933.4         GATGTCGCTGTGGACTTTACCGAGAAGGAGTGGCCCTTGCTGGATTCTTCTCAGAGAAAA
                    ************************************************************

HsCD00329461        CTGTACAAAGATGTAATGCTGGAGAACTACAGCAACCTCACTTCACTGGGGTATCAGGTC
NM_001190791.1      CTGTACAAAGATGTAATGCTGGAGAACTACAGCAACCTCACTTCACTGG-----------
NM_020933.4         CTGTACAAAGATGTAATGCTGGAGAACTACAGCAACCTCACTTCACTGGGGTATCAGGTC
                    *************************************************           

HsCD00329461        GGCAAACCCAGCCTCATCTCACACTTGGAGCAGGAGGAGGAGCCGAGGACAGAGGAGAGG
NM_001190791.1      ------------------------------------------------------------
NM_020933.4         GGCAAACCCAGCCTCATCTCACACTTGGAGCAGGAGGAGGAGCCGAGGACAGAGGAGAGG
                                                                                

HsCD00329461        GGCGCTCACCAGGGCGCTTGTGCAGATTGGGAGACTCCATCTAAAACCAAGTGGTCACTT
NM_001190791.1      -------------------------ATTGGGAGACTCCATCTAAAACCAAGTGGTCACTT
NM_020933.4         GGCGCTCACCAGGGCGCTTGTGCAGATTGGGAGACTCCATCTAAAACCAAGTGGTCACTT
                                             ***********************************

HsCD00329461        CTTATGGAAGATATTTTTGGGAAGGAAACGCCCAGTGGTGTGACGATGGAAAGAGCCGGT
NM_001190791.1      CTTATGGAAGATATTTTTGGGAAGGAAACGCCCAGTGGTGTGACGATGGAAAGAGCCGGT
NM_020933.4         CTTATGGAAGATATTTTTGGGAAGGAAACGCCCAGTGGTGTGACGATGGAAAGAGCCGGT
                    ************************************************************

HsCD00329461        CTTGGAGAGAAGTCCACTGAATACGCTCACTTGTTCGAAGTCTTTGGCATGGACCCTCAT
NM_001190791.1      CTTGGAGAGAAGTCCACTGAATACGCTCACTTGTTCGAAGTCTTTGGCATGGACCCTCAT
NM_020933.4         CTTGGAGAGAAGTCCACTGAATACGCTCACTTGTTCGAAGTCTTTGGCATGGACCCTCAT
                    ************************************************************

HsCD00329461        CTCACTCAGCCAATGGGAAGGCACGCTGGCAAGAGGCCCTATCACCGCCGCGACTATGGG
NM_001190791.1      CTCACTCAGCCAATGGGAAGGCACGCTGGCAAGAGGCCCTATCACCGCCGCGACTATGGG
NM_020933.4         CTCACTCAGCCAATGGGAAGGCACGCTGGCAAGAGGCCCTATCACCGCCGCGACTATGGG
                    ************************************************************

HsCD00329461        GTAGCGTTCAAGGGCAGGCCGCACCTCACTCAGCACATGAGCATGTACGACGGGAGAAAA
NM_001190791.1      GTAGCGTTCAAGGGCAGGCCGCACCTCACTCAGCACATGAGCATGTACGACGGGAGAAAA
NM_020933.4         GTAGCGTTCAAGGGCAGGCCGCACCTCACTCAGCACATGAGCATGTACGACGGGAGAAAA
                    ************************************************************

HsCD00329461        ATGCATGAATGTCATCAGTGCCAAAAAGCCTTCACCACGAGCGCGTCCCTCACACGGCAC
NM_001190791.1      ATGCATGAATGTCATCAGTGCCAAAAAGCCTTCACCACGAGCGCGTCCCTCACACGGCAC
NM_020933.4         ATGCATGAATGTCATCAGTGCCAAAAAGCCTTCACCACGAGCGCGTCCCTCACACGGCAC
                    ************************************************************

HsCD00329461        AGGAGAATCCACACCGGGGAGAAGCCTTACGAGTGCAGCGACTGCGGGAAAGCCTTCAAC
NM_001190791.1      AGGAGAATCCACACCGGGGAGAAGCCTTACGAGTGCAGCGACTGCGGGAAAGCCTTCAAC
NM_020933.4         AGGAGAATCCACACCGGGGAGAAGCCTTACGAGTGCAGCGACTGCGGGAAAGCCTTCAAC
                    ************************************************************

HsCD00329461        GACCCTTCAGCCCTTAGGAGCCACGCAAGAACTCACCTCAAAGAGAAGCCCTTTGACTGC
NM_001190791.1      GACCCTTCAGCCCTTAGGAGCCACGCAAGAACTCACCTCAAAGAGAAGCCCTTTGACTGC
NM_020933.4         GACCCTTCAGCCCTTAGGAGCCACGCAAGAACTCACCTCAAAGAGAAGCCCTTTGACTGC
                    ************************************************************

HsCD00329461        AGTCAGTGTGGAAATGCATTCCGGACCCTCTCGGCCCTGAAAATCCACATGCGAGTTCAC
NM_001190791.1      AGTCAGTGTGGAAATGCATTCCGGACCCTCTCGGCCCTGAAAATCCACATGCGAGTTCAC
NM_020933.4         AGTCAGTGTGGAAATGCATTCCGGACCCTCTCGGCCCTGAAAATCCACATGCGAGTTCAC
                    ************************************************************

HsCD00329461        ACTGGCGAGAGGCCTTACAAGTGTGATCAGTGCGGGAAGGCTTACGGCCGGAGCTGCCAC
NM_001190791.1      ACTGGCGAGAGGCCTTACAAGTGTGATCAGTGCGGGAAGGCTTACGGCCGGAGCTGCCAC
NM_020933.4         ACTGGCGAGAGGCCTTACAAGTGTGATCAGTGCGGGAAGGCTTACGGCCGGAGCTGCCAC
                    ************************************************************

HsCD00329461        CTCATCGCACACAAGAGAACGCACACCGGAGAGAGGCCCTACGAGTGTCACGACTGTGGG
NM_001190791.1      CTCATCGCACACAAGAGAACGCACACCGGAGAGAGGCCCTACGAGTGTCACGACTGTGGG
NM_020933.4         CTCATCGCACACAAGAGAACGCACACCGGAGAGAGGCCCTACGAGTGTCACGACTGTGGG
                    ************************************************************

HsCD00329461        AAAGCTTTCCAGCACCCCTCCCACCTCAAAGAGCACGTGAGGAATCACACGGGGGAGAAG
NM_001190791.1      AAAGCTTTCCAGCACCCCTCCCACCTCAAAGAGCACGTGAGGAATCACACGGGGGAGAAG
NM_020933.4         AAAGCTTTCCAGCACCCCTCCCACCTCAAAGAGCACGTGAGGAATCACACGGGGGAGAAG
                    ************************************************************

HsCD00329461        CCCTACGCGTGCACGCAGTGCGGCAAAGCCTTCCGCTGGAAGTCCAACTTTAATTTGCAC
NM_001190791.1      CCCTACGCGTGCACGCAGTGCGGCAAAGCCTTCCGCTGGAAGTCCAACTTTAATTTGCAC
NM_020933.4         CCCTACGCGTGCACGCAGTGCGGCAAAGCCTTCCGCTGGAAGTCCAACTTTAATTTGCAC
                    ************************************************************

HsCD00329461        AAGAAGAACCACATGGTGGAGAAGACCTACGAATGTAAAGAATGCGGGAAATCCTTTGGC
NM_001190791.1      AAGAAGAACCACATGGTGGAGAAGACCTACGAATGTAAAGAATGCGGGAAATCCTTTGGC
NM_020933.4         AAGAAGAACCACATGGTGGAGAAGACCTACGAATGTAAAGAATGCGGGAAATCCTTTGGC
                    ************************************************************

HsCD00329461        GATCTCGTGTCCCGGAGGAAACACATGAGGATTCACATCGTCAAGAAACCCGTGGAATGT
NM_001190791.1      GATCTCGTGTCCCGGAGGAAACACATGAGGATTCACATCGTCAAGAAACCCGTGGAATGT
NM_020933.4         GATCTCGTGTCCCGGAGGAAACACATGAGGATTCACATCGTCAAGAAACCCGTGGAATGT
                    ************************************************************

HsCD00329461        CGGCAGTGCGGGAAGACCTTCCGAAACCAGTCCATCCTTAAGACTCACATGAACTCTCAC
NM_001190791.1      CGGCAGTGCGGGAAGACCTTCCGAAACCAGTCCATCCTTAAGACTCACATGAACTCTCAC
NM_020933.4         CGGCAGTGCGGGAAGACCTTCCGAAACCAGTCCATCCTTAAGACTCACATGAACTCTCAC
                    ************************************************************

HsCD00329461        ACTGGAGAGAAACCATACGGGTGCGATCTCTGCGGGAAAGCTTTCAGCGCGAGTTCAAAC
NM_001190791.1      ACTGGAGAGAAACCATACGGGTGCGATCTCTGCGGGAAAGCTTTCAGCGCGAGTTCAAAC
NM_020933.4         ACTGGAGAGAAACCATACGGGTGCGATCTCTGCGGGAAAGCTTTCAGCGCGAGTTCAAAC
                    ************************************************************

HsCD00329461        CTCACCGCACACAGGAAGATACACACGCAAGAGAGACGCTACGAATGCGCCGCCTGCGGG
NM_001190791.1      CTCACCGCACACAGGAAGATACACACGCAAGAGAGACGCTACGAATGCGCCGCCTGCGGG
NM_020933.4         CTCACCGCACACAGGAAGATACACACGCAAGAGAGACGCTACGAATGCGCCGCCTGCGGG
                    ************************************************************

HsCD00329461        AAAGTCTTCGGTGACTATTTATCCCGGCGGAGGCACATGAGCGTTCACCTTGTAAAGAAA
NM_001190791.1      AAAGTCTTCGGTGACTATTTATCCCGGCGGAGGCACATGAGCGTTCACCTTGTAAAGAAA
NM_020933.4         AAAGTCTTCGGTGACTATTTATCCCGGCGGAGGCACATGAGCGTTCACCTTGTAAAGAAA
                    ************************************************************

HsCD00329461        CGAGTTGAGTGTAGGCAGTGTGGCAAGGCCTTCAGGAACCAGTCAACGCTGAAGACGCAC
NM_001190791.1      CGAGTTGAGTGTAGGCAGTGTGGCAAGGCCTTCAGGAACCAGTCAACGCTGAAGACGCAC
NM_020933.4         CGAGTTGAGTGTAGGCAGTGTGGCAAGGCCTTCAGGAACCAGTCAACGCTGAAGACGCAC
                    ************************************************************

HsCD00329461        ATGCGAAGCCACACGGGGGAGAAACCGTACGAATGCGATCACTGTGGGAAGGCCTTCAGC
NM_001190791.1      ATGCGAAGCCACACGGGGGAGAAACCGTACGAATGCGATCACTGTGGGAAGGCCTTCAGC
NM_020933.4         ATGCGAAGCCACACGGGGGAGAAACCGTACGAATGCGATCACTGTGGGAAGGCCTTCAGC
                    ************************************************************

HsCD00329461        ATAGGCTCCAACCTGAATGTGCACAGGCGGATCCACACCGGGGAGAAGCCCTACGAATGC
NM_001190791.1      ATAGGCTCCAACCTGAATGTGCACAGGCGGATCCACACCGGGGAGAAGCCCTACGAATGC
NM_020933.4         ATAGGCTCCAACCTGAATGTGCACAGGCGGATCCACACCGGGGAGAAGCCCTACGAATGC
                    ************************************************************

HsCD00329461        CTTGTCTGCGGGAAAGCCTTCAGCGACCACTCATCCCTCAGGAGCCACGTGAAAACTCAC
NM_001190791.1      CTTGTCTGCGGGAAAGCCTTCAGCGACCACTCATCCCTCAGGAGCCACGTGAAAACTCAC
NM_020933.4         CTTGTCTGCGGGAAAGCCTTCAGCGACCACTCATCCCTCAGGAGCCACGTGAAAACTCAC
                    ************************************************************

HsCD00329461        CGGGGAGAGAAGCTCTTTGTGTCATCCGTGTGGAAAAGGCTCCAGTTG
NM_001190791.1      CGGGGAGAGAAGCTCTTTGTGTCATCCGTGTGGAAAAGGCTCCAGTGA
NM_020933.4         CGGGGAGAGAAGCTCTTTGTGTCATCCGTGTGGAAAAGGCTCCAGTGA
                    ********************************************** .

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
SMART domain : KRAB : krueppel associated box
UniProt : Q96PQ6
HPRD : 11706

Clone

Cloning Information : 268348
HIP Master Clone ID : 193206
Original Clone ID : FLH268348.01L

Organism


TAX_ID : 9606
Species Specific ID: 57693

Genome

Chromosome : 19
Map Location : 19p13
Ensembl : ENSG00000130803

Reagents

Labome : ZNF317-antibody