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SNAPC3 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SNAPC3
Gene Name:  small nuclear RNA activating complex, polypeptide 3, 50kDa
Sequence             
Original Clone ID: FLH268365.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329477

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1236nts         Open reading frame : 1 to 1236
Reference Sequence Alignment

ATGGCTGAAGGAAGCCGAGGTGGCCCTACGTGTAGCGGGGTGGGTGGCAGGCAGGACCCA
GTCTCCGGCAGTGGCGGCTGCAACTTTCCAGAGTATGAGCTTCCCGAGCTAAATACGCGC
GCTTTCCATGTGGGCGCCTTTGGGGAGCTGTGGCGGGGCCGTCTGCGCGGGGCCGGGGAC
TTGTCGCTGAGGGAGCCGCCGGCATCCGCTCTGCCTGGGAGCCAGGCAGCTGACTCCGAC
CGGGAGGATGCCGCGGTGGCCAGGGATCTGGACTGCAGCCTGGAGGCGGCGGCTGAGCTG
AGGGCGGTGTGCGGCCTTGATAAACTGAAATGCCTTGAGGACGGTGAGGATCCAGAAGTC
ATTCCGGAGAATACTGACCTGGTGACTTTGGGGGTTAGAAAAAGGTTCTTGGAACATCGG
GAAGAAACCATTACAATAGATCGAGCCTGCAGACAAGAAACATTCGTTTATGAGATGGAG
TCACATGCCATAGGAAAAAAGCCTGAAAATTCAGCAGACATGATTGAAGAAGGGGAGCTT
ATCCTATCTGTGAATATCTTGTACCCTGTTATATTTCATAAGCACAAAGAACACAAACCA
TACCAAACAATGCTGGTGTTGGGCAGTCAAAAACTCACACAACTGAGGGATTCAATTCGA
TGTGTCAGTGACCTCCAGATTGGTGGTGAATTCAGCAACACTCCTGACCAAGCCCCTGAG
CACATCAGCAAAGACCTATACAAATCAGCCTTCTTTTATTTTGAAGGAACATTTTATAAT
GATAAAAGATACCCAGAATGCAGAGATTTGAGCAGAACTATCATTGAGTGGTCAGAGTCC
CATGATAGAGGCTATGGAAAGTTTCAGACTGCTAGAATGGAAGATTTCACCTTCAATGAC
TTGTGTATTAAACTGGGTTTTCCTTACTTATACTGTCATCAGGGAGACTGTGAACATGTC
ATTGTCATTACTGACATAAGGCTTGTGCATCATGATGACTGCTTGGATAGGACATTGTAT
CCCCTCCTTATCAAGAAGCATTGGCTATGGACCAGAAAATGTTTTGTTTGTAAAATGTAT
ACAGCCAGATGGGTGACGAACAATGACAGTTTTGCACCAGAGGACCCATGCTTCTTTTGT
GATGTTTGCTTCCGAATGCTGCACTATGATTCAGAAGGCAACAAACTGGGGGAATTCCTT
GCTTATCCTTATGTTGATCCTGGAACCTTTAATTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1243
Start on reference sequence 8
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SNAPC3
Symbol Nomenclature : SNAPC3
SYNONYM : PTFbeta; SNAP50
Designation : PSE-binding factor subunit beta|PTF subunit beta|SNAPc 50 kDa subunit|SNAPc subunit 3|proximal sequence element-binding transcription factor subunit beta|small nuclear RNA-activating complex polypeptide 3|snRNA-activating protein complex 50 kDa subunit|snRNA-activating protein complex subunit 3
Full Nomenclature : small nuclear RNA activating complex, polypeptide 3, 50kDa
GENEID : 6619
Locus Tag : RP11-307K19.1
HGNC : 11136
MIM : 602348
Vega : OTTHUMG00000019583
Target GenBank: BC108743

 

Reference Sequence Alignment

  

HsCD00329477        ATGGCTGAAGGAAGCCGAGGTGGCCCTACGTGTAGCGGGGTGGGTGGCAGGCAGGACCCA
NM_001039697.1      ATGGCTGAAGGAAGCCGAGGTGGCCCTACGTGTAGCGGGGTGGGTGGCAGGCAGGACCCA
                    ************************************************************

HsCD00329477        GTCTCCGGCAGTGGCGGCTGCAACTTTCCAGAGTATGAGCTTCCCGAGCTAAATACGCGC
NM_001039697.1      GTCTCCGGCAGTGGCGGCTGCAACTTTCCAGAGTATGAGCTTCCCGAGCTAAATACGCGC
                    ************************************************************

HsCD00329477        GCTTTCCATGTGGGCGCCTTTGGGGAGCTGTGGCGGGGCCGTCTGCGCGGGGCCGGGGAC
NM_001039697.1      GCTTTCCATGTGGGCGCCTTTGGGGAGCTGTGGCGGGGCCGTCTGCGCGGGGCCGGGGAC
                    ************************************************************

HsCD00329477        TTGTCGCTGAGGGAGCCGCCGGCATCCGCTCTGCCTGGGAGCCAGGCAGCTGACTCCGAC
NM_001039697.1      TTGTCGCTGAGGGAGCCGCCGGCATCCGCTCTGCCTGGGAGCCAGGCAGCTGACTCCGAC
                    ************************************************************

HsCD00329477        CGGGAGGATGCCGCGGTGGCCAGGGATCTGGACTGCAGCCTGGAGGCGGCGGCTGAGCTG
NM_001039697.1      CGGGAGGATGCCGCGGTGGCCAGGGATCTGGACTGCAGCCTGGAGGCGGCGGCTGAGCTG
                    ************************************************************

HsCD00329477        AGGGCGGTGTGCGGCCTTGATAAACTGAAATGCCTTGAGGACGGTGAGGATCCAGAAGTC
NM_001039697.1      AGGGCGGTGTGCGGCCTTGATAAACTGAAATGCCTTGAGGACGGTGAGGATCCAGAAGTC
                    ************************************************************

HsCD00329477        ATTCCGGAGAATACTGACCTGGTGACTTTGGGGGTTAGAAAAAGGTTCTTGGAACATCGG
NM_001039697.1      ATTCCGGAGAATACTGACCTGGTGACTTTGGGGGTTAGAAAAAGGTTCTTGGAACATCGG
                    ************************************************************

HsCD00329477        GAAGAAACCATTACAATAGATCGAGCCTGCAGACAAGAAACATTCGTTTATGAGATGGAG
NM_001039697.1      GAAGAAACCATTACAATAGATCGAGCCTGCAGACAAGAAACATTCGTTTATGAGATGGAG
                    ************************************************************

HsCD00329477        TCACATGCCATAGGAAAAAAGCCTGAAAATTCAGCAGACATGATTGAAGAAGGGGAGCTT
NM_001039697.1      TCACATGCCATAGGAAAAAAGCCTGAAAATTCAGCAGACATGATTGAAGAAGGGGAGCTT
                    ************************************************************

HsCD00329477        ATCCTATCTGTGAATATCTTGTACCCTGTTATATTTCATAAGCACAAAGAACACAAACCA
NM_001039697.1      ATCCTATCTGTGAATATCTTGTACCCTGTTATATTTCATAAGCACAAAGAACACAAACCA
                    ************************************************************

HsCD00329477        TACCAAACAATGCTGGTGTTGGGCAGTCAAAAACTCACACAACTGAGGGATTCAATTCGA
NM_001039697.1      TACCAAACAATGCTGGTGTTGGGCAGTCAAAAACTCACACAACTGAGGGATTCAATTCGA
                    ************************************************************

HsCD00329477        TGTGTCAGTGACCTCCAGATTGGTGGTGAATTCAGCAACACTCCTGACCAAGCCCCTGAG
NM_001039697.1      TGTGTCAGTGACCTCCAGATTGGTGGTGAATTCAGCAACACTCCTGACCAAGCCCCTGAG
                    ************************************************************

HsCD00329477        CACATCAGCAAAGACCTATACAAATCAGCCTTCTTTTATTTTGAAGGAACATTTTATAAT
NM_001039697.1      CACATCAGCAAAGACCTATACAAATCAGCCTTCTTTTATTTTGAAGGAACATTTTATAAT
                    ************************************************************

HsCD00329477        GATAAAAGATACCCAGAATGCAGAGATTTGAGCAGAACTATCATTGAGTGGTCAGAGTCC
NM_001039697.1      GATAAAAGATACCCAGAATGCAGAGATTTGAGCAGAACTATCATTGAGTGGTCAGAGTCC
                    ************************************************************

HsCD00329477        CATGATAGAGGCTATGGAAAGTTTCAGACTGCTAGAATGGAAGATTTCACCTTCAATGAC
NM_001039697.1      CATGATAGAGGCTATGGAAAGTTTCAGACTGCTAGAATGGAAGATTTCACCTTCAATGAC
                    ************************************************************

HsCD00329477        TTGTGTATTAAACTGGGTTTTCCTTACTTATACTGTCATCAGGGAGACTGTGAACATGTC
NM_001039697.1      TTGTGTATTAAACTGGGTTTTCCTTACTTATACTGTCATCAGGGAGACTGTGAACATGTC
                    ************************************************************

HsCD00329477        ATTGTCATTACTGACATAAGGCTTGTGCATCATGATGACTGCTTGGATAGGACATTGTAT
NM_001039697.1      ATTGTCATTACTGACATAAGGCTTGTGCATCATGATGACTGCTTGGATAGGACATTGTAT
                    ************************************************************

HsCD00329477        CCCCTCCTTATCAAGAAGCATTGGCTATGGACCAGAAAATGTTTTGTTTGTAAAATGTAT
NM_001039697.1      CCCCTCCTTATCAAGAAGCATTGGCTATGGACCAGAAAATGTTTTGTTTGTAAAATGTAT
                    ************************************************************

HsCD00329477        ACAGCCAGATGGGTGACGAACAATGACAGTTTTGCACCAGAGGACCCATGCTTCTTTTGT
NM_001039697.1      ACAGCCAGATGGGTGACGAACAATGACAGTTTTGCACCAGAGGACCCATGCTTCTTTTGT
                    ************************************************************

HsCD00329477        GATGTTTGCTTCCGAATGCTGCACTATGATTCAGAAGGCAACAAACTGGGGGAATTCCTT
NM_001039697.1      GATGTTTGCTTCCGAATGCTGCACTATGATTCAGAAGGCAACAAACTGGGGGAATTCCTT
                    ************************************************************

HsCD00329477        GCTTATCCTTATGTTGATCCTGGAACCTTTAATTTG
NM_001039697.1      GCTTATCCTTATGTTGATCCTGGAACCTTTAATTAA
                    **********************************:.

Pathways

Reactome : Gene Expression
Reactome : RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
Reactome : RNA Polymerase III Abortive And Retractive Initiation
Reactome : RNA Polymerase III Transcription
Reactome : RNA Polymerase III Transcription Initiation
Reactome : RNA Polymerase III Transcription Initiation From Type 3 Promoter
Reactome : Transcription

Protein

UniProt : Q92966
HPRD : 03826

Clone

Cloning Information : 268365
HIP Master Clone ID : 193227
Original Clone ID : FLH268365.01L

Organism


TAX_ID : 9606
Species Specific ID: 6619

Genome

Chromosome : 9
Map Location : 9p22.3
Ensembl : ENSG00000164975

Reagents

Labome : SNAPC3-antibody