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CBX5 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CBX5
Gene Name:  chromobox homolog 5
Sequence             
Original Clone ID: FLH268389.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329499

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 576nts         Open reading frame : 1 to 576
Reference Sequence Alignment

ATGGGAAAGAAAACCAAGCGGACAGCTGACAGTTCTTCTTCAGAGGATGAGGAGGAGTAT
GTTGTGGAGAAGGTGCTAGACAGGCGCGTGGTTAAGGGACAAGTGGAATATCTACTGAAG
TGGAAAGGCTTTTCTGAGGAGCACAATACTTGGGAACCTGAGAAAAACTTGGATTGCCCT
GAGCTAATTTCTGAATTTATGAAAAAGTATAAGAAGATGAAGGAGGGTGAAAATAATAAA
CCCAGGGAGAAGTCAGAAAGTAACAAGAGGAAATCCAATTTCTCAAACAGTGCCGATGAC
ATCAAATCTAAAAAAAAGAGAGAGCAGAGCAATGATATCGCTCGGGGCTTTGAGAGAGGA
CTGGAACCAGAAAAGATCATTGGGGCAACAGATTCCTGTGGTGATTTAATGTTCCTAATG
AAATGGAAAGACACAGATGAAGCTGACCTGGTTCTTGCAAAAGAAGCTAATGTGAAATGT
CCACAAATTGTGATAGCATTTTATGAAGAGAGACTGACATGGCATGCATATCCTGAGGAT
GCGGAAAACAAAGAGAAAGAAACAGCAAAGAGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 644
Start on reference sequence 69
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CBX5
Symbol Nomenclature : CBX5
SYNONYM : HEL25; HP1; HP1A
Designation : HP1 alpha homolog|HP1-ALPHA|HP1Hs alpha|antigen p25|chromobox homolog 5 (HP1 alpha homolog, Drosophila)|chromobox protein homolog 5|epididymis luminal protein 25|heterochromatin protein 1 homolog alpha|heterochromatin protein 1-alpha
Full Nomenclature : chromobox homolog 5
GENEID : 23468
HGNC : 1555
MIM : 604478
Vega : OTTHUMG00000169700
Target GenBank: BC006821

 

Reference Sequence Alignment

  

HsCD00329499        ATGGGAAAGAAAACCAAGCGGACAGCTGACAGTTCTTCTTCAGAGGATGAGGAGGAGTAT
NM_001127321.1      ATGGGAAAGAAAACCAAGCGGACAGCTGACAGTTCTTCTTCAGAGGATGAGGAGGAGTAT
NM_001127322.1      ATGGGAAAGAAAACCAAGCGGACAGCTGACAGTTCTTCTTCAGAGGATGAGGAGGAGTAT
NM_012117.2         ATGGGAAAGAAAACCAAGCGGACAGCTGACAGTTCTTCTTCAGAGGATGAGGAGGAGTAT
                    ************************************************************

HsCD00329499        GTTGTGGAGAAGGTGCTAGACAGGCGCGTGGTTAAGGGACAAGTGGAATATCTACTGAAG
NM_001127321.1      GTTGTGGAGAAGGTGCTAGACAGGCGCGTGGTTAAGGGACAAGTGGAATATCTACTGAAG
NM_001127322.1      GTTGTGGAGAAGGTGCTAGACAGGCGCGTGGTTAAGGGACAAGTGGAATATCTACTGAAG
NM_012117.2         GTTGTGGAGAAGGTGCTAGACAGGCGCGTGGTTAAGGGACAAGTGGAATATCTACTGAAG
                    ************************************************************

HsCD00329499        TGGAAAGGCTTTTCTGAGGAGCACAATACTTGGGAACCTGAGAAAAACTTGGATTGCCCT
NM_001127321.1      TGGAAAGGCTTTTCTGAGGAGCACAATACTTGGGAACCTGAGAAAAACTTGGATTGCCCT
NM_001127322.1      TGGAAAGGCTTTTCTGAGGAGCACAATACTTGGGAACCTGAGAAAAACTTGGATTGCCCT
NM_012117.2         TGGAAAGGCTTTTCTGAGGAGCACAATACTTGGGAACCTGAGAAAAACTTGGATTGCCCT
                    ************************************************************

HsCD00329499        GAGCTAATTTCTGAATTTATGAAAAAGTATAAGAAGATGAAGGAGGGTGAAAATAATAAA
NM_001127321.1      GAGCTAATTTCTGAATTTATGAAAAAGTATAAGAAGATGAAGGAGGGTGAAAATAATAAA
NM_001127322.1      GAGCTAATTTCTGAATTTATGAAAAAGTATAAGAAGATGAAGGAGGGTGAAAATAATAAA
NM_012117.2         GAGCTAATTTCTGAATTTATGAAAAAGTATAAGAAGATGAAGGAGGGTGAAAATAATAAA
                    ************************************************************

HsCD00329499        CCCAGGGAGAAGTCAGAAAGTAACAAGAGGAAATCCAATTTCTCAAACAGTGCCGATGAC
NM_001127321.1      CCCAGGGAGAAGTCAGAAAGTAACAAGAGGAAATCCAATTTCTCAAACAGTGCCGATGAC
NM_001127322.1      CCCAGGGAGAAGTCAGAAAGTAACAAGAGGAAATCCAATTTCTCAAACAGTGCCGATGAC
NM_012117.2         CCCAGGGAGAAGTCAGAAAGTAACAAGAGGAAATCCAATTTCTCAAACAGTGCCGATGAC
                    ************************************************************

HsCD00329499        ATCAAATCTAAAAAAAAGAGAGAGCAGAGCAATGATATCGCTCGGGGCTTTGAGAGAGGA
NM_001127321.1      ATCAAATCTAAAAAAAAGAGAGAGCAGAGCAATGATATCGCTCGGGGCTTTGAGAGAGGA
NM_001127322.1      ATCAAATCTAAAAAAAAGAGAGAGCAGAGCAATGATATCGCTCGGGGCTTTGAGAGAGGA
NM_012117.2         ATCAAATCTAAAAAAAAGAGAGAGCAGAGCAATGATATCGCTCGGGGCTTTGAGAGAGGA
                    ************************************************************

HsCD00329499        CTGGAACCAGAAAAGATCATTGGGGCAACAGATTCCTGTGGTGATTTAATGTTCCTAATG
NM_001127321.1      CTGGAACCAGAAAAGATCATTGGGGCAACAGATTCCTGTGGTGATTTAATGTTCCTAATG
NM_001127322.1      CTGGAACCAGAAAAGATCATTGGGGCAACAGATTCCTGTGGTGATTTAATGTTCCTAATG
NM_012117.2         CTGGAACCAGAAAAGATCATTGGGGCAACAGATTCCTGTGGTGATTTAATGTTCCTAATG
                    ************************************************************

HsCD00329499        AAATGGAAAGACACAGATGAAGCTGACCTGGTTCTTGCAAAAGAAGCTAATGTGAAATGT
NM_001127321.1      AAATGGAAAGACACAGATGAAGCTGACCTGGTTCTTGCAAAAGAAGCTAATGTGAAATGT
NM_001127322.1      AAATGGAAAGACACAGATGAAGCTGACCTGGTTCTTGCAAAAGAAGCTAATGTGAAATGT
NM_012117.2         AAATGGAAAGACACAGATGAAGCTGACCTGGTTCTTGCAAAAGAAGCTAATGTGAAATGT
                    ************************************************************

HsCD00329499        CCACAAATTGTGATAGCATTTTATGAAGAGAGACTGACATGGCATGCATATCCTGAGGAT
NM_001127321.1      CCACAAATTGTGATAGCATTTTATGAAGAGAGACTGACATGGCATGCATATCCTGAGGAT
NM_001127322.1      CCACAAATTGTGATAGCATTTTATGAAGAGAGACTGACATGGCATGCATATCCTGAGGAT
NM_012117.2         CCACAAATTGTGATAGCATTTTATGAAGAGAGACTGACATGGCATGCATATCCTGAGGAT
                    ************************************************************

HsCD00329499        GCGGAAAACAAAGAGAAAGAAACAGCAAAGAGCTTG
NM_001127321.1      GCGGAAAACAAAGAGAAAGAAACAGCAAAGAGCTAA
NM_001127322.1      GCGGAAAACAAAGAGAAAGAAACAGCAAAGAGCTAA
NM_012117.2         GCGGAAAACAAAGAGAAAGAAACAGCAAAGAGCTAA
                    **********************************:.

Pathways

NCI : Aurora B signaling
NCI : E2F transcription factor network
Reactome : Factors involved in megakaryocyte development and platelet production
Reactome : Hemostasis

Protein

SMART domain : CHROMO : Chromatin organization modifier domain
SMART domain : ChSh : Chromo Shadow Domain
UniProt : P45973
HPRD : 05131

Clone

Cloning Information : 268389
HIP Master Clone ID : 194868
Original Clone ID : FLH268389.01L

Organism


TAX_ID : 9606
Species Specific ID: 23468

Genome

Chromosome : 12
Map Location : 12q13.13
Ensembl : ENSG00000094916

Reagents

Labome : CBX5-antibody