DNASU Plasmid Repository • 480.965.5697 | Email

TCEAL2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  TCEAL2
Gene Name:  transcription elongation factor A (SII)-like 2
Sequence             
Original Clone ID: FLH268398.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329508

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 684nts         Open reading frame : 1 to 684
Reference Sequence Alignment

ATGGAAAAACTCTTCAATGAAAATGAAGGAATGCCTTCGAATCAAGGAAAGATAGACAAT
GAAGAACAGCCACCGCACGAGGGAAAGCCAGAAGTAGCTTGTATTCTGGAAGACAAGAAG
TTAGAAAACGAGGGAAACACAGAAAACACGGGCAAGAGAGTTGAGGAACCGTTAAAGGAT
AAAGAAAAGCCAGAGAGTGCGGGAAAGGCAAAAGGAGAAGGAAAGTCAGAGAGGAAGGGA
AAGTCAGAGATGCAGGGAGGATCAAAGACAGAGGGAAAGCCAGAGAGAGGGGGAAGGGCA
GAGGGTGAAGGAGAGCCAGACAGTGAAAGAGAGCCAGAGAGTGAGGGAGAGCCAGAAAGT
GAAACAAGGGCTGCAGGAAAGCGCCCAGCTGAGGATGATATACCCAGGAAAGCCAAAAGA
AAAACCAACAAGGGGCTGGCTCAGTACCTCAAGCAATATAAGGAAGCCATACATGATATG
AATTTCAGCAATGAGGACATGATAAGAGAATTTGACAACATGGCTAGGGTGGAGGATAAA
AGGAGAAAAAGCAAACAGAAATTGGGGGCGTTTTTGTGGATGCAAAGAAATTTACAGGAC
CCCTTCTATCCTAGGGGTCCAAGGGAATTCAGGGGTGGCTGCAGGGCCCCACGAAGGGAC
ACTGAAGACATTCCTTATGTGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 782
Start on reference sequence 99
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TCEAL2
Symbol Nomenclature : TCEAL2
SYNONYM : MY0876G05; my048
Designation : TCEA-like protein 2|transcription elongation factor A protein-like 2|transcription elongation factor S-II protein-like 2
Full Nomenclature : transcription elongation factor A (SII)-like 2
GENEID : 140597
Locus Tag : My048
HGNC : 29818
Vega : OTTHUMG00000022048
Target GenBank: BC104764

 

Reference Sequence Alignment

  

HsCD00329508      ATGGAAAAACTCTTCAATGAAAATGAAGGAATGCCTTCGAATCAAGGAAAGATAGACAAT
NM_080390.3       ATGGAAAAACTCTTCAATGAAAATGAAGGAATGCCTTCGAATCAAGGAAAGATAGACAAT
                  ************************************************************

HsCD00329508      GAAGAACAGCCACCGCACGAGGGAAAGCCAGAAGTAGCTTGTATTCTGGAAGACAAGAAG
NM_080390.3       GAAGAACAGCCACCGCACGAGGGAAAGCCAGAAGTAGCTTGTATTCTGGAAGACAAGAAG
                  ************************************************************

HsCD00329508      TTAGAAAACGAGGGAAACACAGAAAACACGGGCAAGAGAGTTGAGGAACCGTTAAAGGAT
NM_080390.3       TTAGAAAACGAGGGAAACACAGAAAACACGGGCAAGAGAGTTGAGGAACCGTTAAAGGAT
                  ************************************************************

HsCD00329508      AAAGAAAAGCCAGAGAGTGCGGGAAAGGCAAAAGGAGAAGGAAAGTCAGAGAGGAAGGGA
NM_080390.3       AAAGAAAAGCCAGAGAGTGCGGGAAAGGCAAAAGGAGAAGGAAAGTCAGAGAGGAAGGGA
                  ************************************************************

HsCD00329508      AAGTCAGAGATGCAGGGAGGATCAAAGACAGAGGGAAAGCCAGAGAGAGGGGGAAGGGCA
NM_080390.3       AAGTCAGAGATGCAGGGAGGATCAAAGACAGAGGGAAAGCCAGAGAGAGGGGGAAGGGCA
                  ************************************************************

HsCD00329508      GAGGGTGAAGGAGAGCCAGACAGTGAAAGAGAGCCAGAGAGTGAGGGAGAGCCAGAAAGT
NM_080390.3       GAGGGTGAAGGAGAGCCAGACAGTGAAAGAGAGCCAGAGAGTGAGGGAGAGCCAGAAAGT
                  ************************************************************

HsCD00329508      GAAACAAGGGCTGCAGGAAAGCGCCCAGCTGAGGATGATATACCCAGGAAAGCCAAAAGA
NM_080390.3       GAAACAAGGGCTGCAGGAAAGCGCCCAGCTGAGGATGATATACCCAGGAAAGCCAAAAGA
                  ************************************************************

HsCD00329508      AAAACCAACAAGGGGCTGGCTCAGTACCTCAAGCAATATAAGGAAGCCATACATGATATG
NM_080390.3       AAAACCAACAAGGGGCTGGCTCAGTACCTCAAGCAATATAAGGAAGCCATACATGATATG
                  ************************************************************

HsCD00329508      AATTTCAGCAATGAGGACATGATAAGAGAATTTGACAACATGGCTAGGGTGGAGGATAAA
NM_080390.3       AATTTCAGCAATGAGGACATGATAAGAGAATTTGACAACATGGCTAGGGTGGAGGATAAA
                  ************************************************************

HsCD00329508      AGGAGAAAAAGCAAACAGAAATTGGGGGCGTTTTTGTGGATGCAAAGAAATTTACAGGAC
NM_080390.3       AGGAGAAAAAGCAAACAGAAATTGGGGGCGTTTTTGTGGATGCAAAGAAATTTACAGGAC
                  ************************************************************

HsCD00329508      CCCTTCTATCCTAGGGGTCCAAGGGAATTCAGGGGTGGCTGCAGGGCCCCACGAAGGGAC
NM_080390.3       CCCTTCTATCCTAGGGGTCCAAGGGAATTCAGGGGTGGCTGCAGGGCCCCACGAAGGGAC
                  ************************************************************

HsCD00329508      ACTGAAGACATTCCTTATGTGTTG
NM_080390.3       ACTGAAGACATTCCTTATGTGTAG
                  **********************:*

Protein

UniProt : Q9H3H9
HPRD : 06761

Clone

Cloning Information : 268398
HIP Master Clone ID : 194882
Original Clone ID : FLH268398.01L

Organism


TAX_ID : 9606
Species Specific ID: 140597

Genome

Chromosome : X
Map Location : Xq22.1-q22.3
Ensembl : ENSG00000184905

Reagents

Labome : TCEAL2-antibody