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ENC2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHL25
Gene Name:  kelch-like family member 25
Sequence             
Original Clone ID: FLH268407.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329517

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1770nts         Open reading frame : 1 to 1770
Reference Sequence Alignment

ATGTCGGTCAGTGTCCATGAGACCCGCAAGTCGCGGAGCAGCACGGGGTCCATGAACGTC
ACCCTCTTCCACAAGGCCTCCCACCCGGACTGTGTGCTGGCCCACCTCAACACGCTTCGC
AAGCACTGCATGTTCACCGACGTCACACTCTGGGCGGGCGACCGTGCCTTCCCCTGTCAC
CGTGCCGTGCTGGCCGCCTCTAGCCGCTATTTTGAGGCCATGTTCAGCCATGGCCTTCGG
GAGAGCCGGGATGACACTGTCAACTTCCAGGACAACCTGCACCCGGAGGTGCTGGAGCTG
CTGCTGGACTTTGCCTACTCCTCACGCATCGCCATCAACGAGGAGAACGCTGAGTCACTG
CTGGAGGCAGGCGACATGCTGCAGTTCCACGATGTGCGGGATGCTGCCGCCGAGTTCCTG
GAGAAGAACCTTTTCCCCTCCAACTGCCTGGGCATGATGCTGCTCTCGGACGCCCACCAG
TGCCGCCGGCTGTATGAGTTCTCCTGGCGCATGTGCCTGGTGCACTTTGAGACGGTGAGG
CAGAGCGAGGACTTCAACAGCCTGTCCAAGGACACACTGCTGGACCTCATCTCGAGTGAT
GAGCTGGAGACCGAGGACGAGCGGGTGGTCTTCGAGGCCATCCTCCAGTGGGTGAAGCAC
GACCTGGAGCCACGGAAGGTCCACTTGCCCGAGCTCCTCCGCAGCGTGCGTCTGGCCTTG
CTGCCGTCCGACTGCCTGCAGGAGGCCGTCTCCAGCGAGGCCCTCCTCATGGCAGACGAG
CGCACCAAGCTTATCATGGATGAGGCCCTGCGCTGCAAGACCAGGATCCTGCAGAATGAT
GGCGTGGTCACCAGCCCCTGTGCCCGGCCACGCAAGGCGGGCCACACGCTACTCATCCTG
GGGGGCCAGACCTTCATGTGTGACAAGATCTACCAGGTGGACCACAAGGCCAAGGAGATC
ATCCCCAAGGCCGACCTGCCCAGCCCCCGGAAGGAGTTCAGCGCCTCAGCGATCGGCTGC
AAGGTCTATGTGACGGGGGGCAGGGGCTCCGAGAACGGGGTCTCCAAGGATGTCTGGGTG
TACGACACCGTACATGAGGAATGGTCCAAGGCGGCGCCCATGCTGATTGCCCGCTTTGGC
CATGGCTCAGCTGAGCTGGAGAACTGCCTCTATGTGGTGGGGGGACACACATCCCTGGCA
GGGGTCTTCCCGGCCTCGCCTTCTGTCTCCCTGAAACAAGTGGAGAAATACGACCCTGGG
GCCAACAAGTGGATGATGGTGGCCCCCTTGCGGGATGGCGTCAGCAATGCCGCAGTGGTG
AGTGCCAAGCTGAAGCTCTTTGTTTTCGGAGGAACCAGCATCCACCGGGACATGGTGTCC
AAGGTCCAGTGCTATGACCCCTCGGAGAACAGGTGGACGATCAAGGCCGAGTGCCCCCAG
CCTTGGCGGTACACAGCCGCTGCCGTCCTGGGCAGCCAGATCTTCATCATGGGAGGTGAC
ACGGAATTCACAGCCGCCTCGGCCTACCGCTTTGACTGTGAGACCAACCAGTGGACGCGG
ATTGGGGACATGACTGCCAAGCGCATGTCCTGCCATGCCCTGGCTTCCGGCAACAAGCTC
TATGTGGTCGGGGGCTACTTTGGGACCCAGAGGTGTAAGACTCTGGACTGCTATGACCCC
ACTTCAGATACATGGAACTGCATCACCACAGTGCCCTACTCACTTATCCCCACGGCCTTT
GTCAGCACCTGGAAGCACCTGCCCGCGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1892
Start on reference sequence 123
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHL25
Symbol Nomenclature : KLHL25
SYNONYM : ENC-2; ENC2
Designation : BTB/POZ KELCH domain protein|ectoderm-neural cortex protein 2|ectodermal-neural cortex 2|kelch-like 25|kelch-like protein 25
Full Nomenclature : kelch-like family member 25
GENEID : 64410
HGNC : 25732
Vega : OTTHUMG00000148672
Target GenBank: BC028100

 

Reference Sequence Alignment

  

HsCD00329517      ATGTCGGTCAGTGTCCATGAGACCCGCAAGTCGCGGAGCAGCACGGGGTCCATGAACGTC
NM_022480.3       ATGTCGGTCAGTGTCCATGAGACCCGCAAGTCGCGGAGCAGCACGGGGTCCATGAACGTC
                  ************************************************************

HsCD00329517      ACCCTCTTCCACAAGGCCTCCCACCCGGACTGTGTGCTGGCCCACCTCAACACGCTTCGC
NM_022480.3       ACCCTCTTCCACAAGGCCTCCCACCCGGACTGTGTGCTGGCCCACCTCAACACGCTTCGC
                  ************************************************************

HsCD00329517      AAGCACTGCATGTTCACCGACGTCACACTCTGGGCGGGCGACCGTGCCTTCCCCTGTCAC
NM_022480.3       AAGCACTGCATGTTCACCGACGTCACACTCTGGGCGGGCGACCGTGCCTTCCCCTGTCAC
                  ************************************************************

HsCD00329517      CGTGCCGTGCTGGCCGCCTCTAGCCGCTATTTTGAGGCCATGTTCAGCCATGGCCTTCGG
NM_022480.3       CGTGCCGTGCTGGCCGCCTCTAGCCGCTATTTTGAGGCCATGTTCAGCCATGGCCTTCGG
                  ************************************************************

HsCD00329517      GAGAGCCGGGATGACACTGTCAACTTCCAGGACAACCTGCACCCGGAGGTGCTGGAGCTG
NM_022480.3       GAGAGCCGGGATGACACTGTCAACTTCCAGGACAACCTGCACCCGGAGGTGCTGGAGCTG
                  ************************************************************

HsCD00329517      CTGCTGGACTTTGCCTACTCCTCACGCATCGCCATCAACGAGGAGAACGCTGAGTCACTG
NM_022480.3       CTGCTGGACTTTGCCTACTCCTCACGCATCGCCATCAACGAGGAGAACGCTGAGTCACTG
                  ************************************************************

HsCD00329517      CTGGAGGCAGGCGACATGCTGCAGTTCCACGATGTGCGGGATGCTGCCGCCGAGTTCCTG
NM_022480.3       CTGGAGGCAGGCGACATGCTGCAGTTCCACGATGTGCGGGATGCTGCCGCCGAGTTCCTG
                  ************************************************************

HsCD00329517      GAGAAGAACCTTTTCCCCTCCAACTGCCTGGGCATGATGCTGCTCTCGGACGCCCACCAG
NM_022480.3       GAGAAGAACCTTTTCCCCTCCAACTGCCTGGGCATGATGCTGCTCTCGGACGCCCACCAG
                  ************************************************************

HsCD00329517      TGCCGCCGGCTGTATGAGTTCTCCTGGCGCATGTGCCTGGTGCACTTTGAGACGGTGAGG
NM_022480.3       TGCCGCCGGCTGTATGAGTTCTCCTGGCGCATGTGCCTGGTGCACTTTGAGACGGTGAGG
                  ************************************************************

HsCD00329517      CAGAGCGAGGACTTCAACAGCCTGTCCAAGGACACACTGCTGGACCTCATCTCGAGTGAT
NM_022480.3       CAGAGCGAGGACTTCAACAGCCTGTCCAAGGACACACTGCTGGACCTCATCTCGAGTGAT
                  ************************************************************

HsCD00329517      GAGCTGGAGACCGAGGACGAGCGGGTGGTCTTCGAGGCCATCCTCCAGTGGGTGAAGCAC
NM_022480.3       GAGCTGGAGACCGAGGACGAGCGGGTGGTCTTCGAGGCCATCCTCCAGTGGGTGAAGCAC
                  ************************************************************

HsCD00329517      GACCTGGAGCCACGGAAGGTCCACTTGCCCGAGCTCCTCCGCAGCGTGCGTCTGGCCTTG
NM_022480.3       GACCTGGAGCCACGGAAGGTCCACTTGCCCGAGCTCCTCCGCAGCGTGCGTCTGGCCTTG
                  ************************************************************

HsCD00329517      CTGCCGTCCGACTGCCTGCAGGAGGCCGTCTCCAGCGAGGCCCTCCTCATGGCAGACGAG
NM_022480.3       CTGCCGTCCGACTGCCTGCAGGAGGCCGTCTCCAGCGAGGCCCTCCTCATGGCAGACGAG
                  ************************************************************

HsCD00329517      CGCACCAAGCTTATCATGGATGAGGCCCTGCGCTGCAAGACCAGGATCCTGCAGAATGAT
NM_022480.3       CGCACCAAGCTTATCATGGATGAGGCCCTGCGCTGCAAGACCAGGATCCTGCAGAATGAT
                  ************************************************************

HsCD00329517      GGCGTGGTCACCAGCCCCTGTGCCCGGCCACGCAAGGCGGGCCACACGCTACTCATCCTG
NM_022480.3       GGCGTGGTCACCAGCCCCTGTGCCCGGCCACGCAAGGCGGGCCACACGCTACTCATCCTG
                  ************************************************************

HsCD00329517      GGGGGCCAGACCTTCATGTGTGACAAGATCTACCAGGTGGACCACAAGGCCAAGGAGATC
NM_022480.3       GGGGGCCAGACCTTCATGTGTGACAAGATCTACCAGGTGGACCACAAGGCCAAGGAGATC
                  ************************************************************

HsCD00329517      ATCCCCAAGGCCGACCTGCCCAGCCCCCGGAAGGAGTTCAGCGCCTCAGCGATCGGCTGC
NM_022480.3       ATCCCCAAGGCCGACCTGCCCAGCCCCCGGAAGGAGTTCAGCGCCTCAGCGATCGGCTGC
                  ************************************************************

HsCD00329517      AAGGTCTATGTGACGGGGGGCAGGGGCTCCGAGAACGGGGTCTCCAAGGATGTCTGGGTG
NM_022480.3       AAGGTCTATGTGACGGGGGGCAGGGGCTCCGAGAACGGGGTCTCCAAGGATGTCTGGGTG
                  ************************************************************

HsCD00329517      TACGACACCGTACATGAGGAATGGTCCAAGGCGGCGCCCATGCTGATTGCCCGCTTTGGC
NM_022480.3       TACGACACCGTACATGAGGAATGGTCCAAGGCGGCGCCCATGCTGATTGCCCGCTTTGGC
                  ************************************************************

HsCD00329517      CATGGCTCAGCTGAGCTGGAGAACTGCCTCTATGTGGTGGGGGGACACACATCCCTGGCA
NM_022480.3       CATGGCTCAGCTGAGCTGGAGAACTGCCTCTATGTGGTGGGGGGACACACATCCCTGGCA
                  ************************************************************

HsCD00329517      GGGGTCTTCCCGGCCTCGCCTTCTGTCTCCCTGAAACAAGTGGAGAAATACGACCCTGGG
NM_022480.3       GGGGTCTTCCCGGCCTCGCCTTCTGTCTCCCTGAAACAAGTGGAGAAATACGACCCTGGG
                  ************************************************************

HsCD00329517      GCCAACAAGTGGATGATGGTGGCCCCCTTGCGGGATGGCGTCAGCAATGCCGCAGTGGTG
NM_022480.3       GCCAACAAGTGGATGATGGTGGCCCCCTTGCGGGATGGCGTCAGCAATGCCGCAGTGGTG
                  ************************************************************

HsCD00329517      AGTGCCAAGCTGAAGCTCTTTGTTTTCGGAGGAACCAGCATCCACCGGGACATGGTGTCC
NM_022480.3       AGTGCCAAGCTGAAGCTCTTTGTTTTCGGAGGAACCAGCATCCACCGGGACATGGTGTCC
                  ************************************************************

HsCD00329517      AAGGTCCAGTGCTATGACCCCTCGGAGAACAGGTGGACGATCAAGGCCGAGTGCCCCCAG
NM_022480.3       AAGGTCCAGTGCTATGACCCCTCGGAGAACAGGTGGACGATCAAGGCCGAGTGCCCCCAG
                  ************************************************************

HsCD00329517      CCTTGGCGGTACACAGCCGCTGCCGTCCTGGGCAGCCAGATCTTCATCATGGGAGGTGAC
NM_022480.3       CCTTGGCGGTACACAGCCGCTGCCGTCCTGGGCAGCCAGATCTTCATCATGGGAGGTGAC
                  ************************************************************

HsCD00329517      ACGGAATTCACAGCCGCCTCGGCCTACCGCTTTGACTGTGAGACCAACCAGTGGACGCGG
NM_022480.3       ACGGAATTCACAGCCGCCTCGGCCTACCGCTTTGACTGTGAGACCAACCAGTGGACGCGG
                  ************************************************************

HsCD00329517      ATTGGGGACATGACTGCCAAGCGCATGTCCTGCCATGCCCTGGCTTCCGGCAACAAGCTC
NM_022480.3       ATTGGGGACATGACTGCCAAGCGCATGTCCTGCCATGCCCTGGCTTCCGGCAACAAGCTC
                  ************************************************************

HsCD00329517      TATGTGGTCGGGGGCTACTTTGGGACCCAGAGGTGTAAGACTCTGGACTGCTATGACCCC
NM_022480.3       TATGTGGTCGGGGGCTACTTTGGGACCCAGAGGTGTAAGACTCTGGACTGCTATGACCCC
                  ************************************************************

HsCD00329517      ACTTCAGATACATGGAACTGCATCACCACAGTGCCCTACTCACTTATCCCCACGGCCTTT
NM_022480.3       ACTTCAGATACATGGAACTGCATCACCACAGTGCCCTACTCACTTATCCCCACGGCCTTT
                  ************************************************************

HsCD00329517      GTCAGCACCTGGAAGCACCTGCCCGCGTTG
NM_022480.3       GTCAGCACCTGGAAGCACCTGCCCGCGTGA
                  **************************** .

Protein

SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : Kelch :
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
UniProt : Q9H0H3
HPRD : 07780

Clone

Cloning Information : 268407
HIP Master Clone ID : 194901
Original Clone ID : FLH268407.01L

Organism


TAX_ID : 9606
Species Specific ID: 64410

Genome

Chromosome : 15
Map Location : 15q25.3
Ensembl : ENSG00000183655

Reagents

Labome : KLHL25-antibody