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GTF2H3 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  GTF2H3
Gene Name:  general transcription factor IIH, polypeptide 3, 34kDa
Sequence             
Original Clone ID: FLH268411.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00329521

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 927nts         Open reading frame : 1 to 927
Reference Sequence Alignment

ATGGTTTCAGACGAAGATGAATTGAATCTTCTGGTTATTGTAGTTGATGCCAACCCAATT
TGGTGGGGAAAGCAAGCATTACAGGAATCTCAGTTCACTTTATCCAAATGCATAGATGCC
GTGATGGTGCTGGGAAATTCGCATTTATTCATGAATCGTTCCAACAAACTTGCTGTGATA
GCAAGTCACATTCAAGAAAGCCGATTCTTATATCCTGGAAAGAATGGCAGACTTGGAGAC
TTCTTCGGAGACCCTGGCAACCCTCCTGAATTTAATCCCTCTGGGAGTAAAGATGGAAAA
TACGAACTTTTAACCTCAGCAAATGAAGTTATTGTTGAAGAGATTAAAGATCTAATGACC
AAAAGTGACATAAAGGGTCAACATACAGAAACTTTGCTGGCAGGATCCCTGGCCAAAGCC
CTTTGCTACATTCATAGAATGAACAAGGAAGTTAAAGACAATCAGGAAATGAAATCAAGG
ATATTGGTGATTAAGGCTGCAGAAGACAGTGCGTTGCAGTATATGAACTTCATGAATGTC
ATCTTTGCAGCACAGAAACAGAATATTTTGATTGATGCCTGTGTTTTAGACTCCGACTCA
GGGCTCCTCCAACAGGCTTGTGACATCACGGGAGGACTGTACCTGAAGGTGCCTCAGATG
CCTTCTCTTCTGCAGTATTTGCTGTGGGTGTTTCTTCCCGATCAAGATCAGAGATCTCAG
TTAATCCTCCCACCCCCGGTTCATGTTGACTACAGGGCTGCTTGCTTCTGTCATCGAAAT
CTCATTGAAATTGGTTATGTCTGTTCTGTGTGTTTGTCAATATTCTGCAATTTCAGCCCC
ATTTGTACTACGTGCGAGACAGCCTTTAAAATTTCTCTGCCTCCAGTGCTGAAAGCCAAG
AAAAAGAAACTGAAAGTGTCTGCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 941
Start on reference sequence 15
Mutations:
None
Discrepancy:
a82c,K28Q
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GTF2H3
Symbol Nomenclature : GTF2H3
SYNONYM : BTF2; P34; TFB4; TFIIH
Designation : TFIIH basal transcription factor complex p34 subunit|basic transcription factor 2 34 kDa subunit|general transcription factor IIH subunit 3
Full Nomenclature : general transcription factor IIH, polypeptide 3, 34kDa
GENEID : 2967
HGNC : 4657
MIM : 601750
Vega : OTTHUMG00000168697
Target GenBank: BC065250

 

Reference Sequence Alignment

  

HsCD00329521      ATGGTTTCAGACGAAGATGAATTGAATCTTCTGGTTATTGTAGTTGATGCCAACCCAATT
NM_001516.4       ATGGTTTCAGACGAAGATGAATTGAATCTTCTGGTTATTGTAGTTGATGCCAACCCAATT
                  ************************************************************

HsCD00329521      TGGTGGGGAAAGCAAGCATTACAGGAATCTCAGTTCACTTTATCCAAATGCATAGATGCC
NM_001516.4       TGGTGGGGAAAGCAAGCATTAAAGGAATCTCAGTTCACTTTATCCAAATGCATAGATGCC
                  *********************.**************************************

HsCD00329521      GTGATGGTGCTGGGAAATTCGCATTTATTCATGAATCGTTCCAACAAACTTGCTGTGATA
NM_001516.4       GTGATGGTGCTGGGAAATTCGCATTTATTCATGAATCGTTCCAACAAACTTGCTGTGATA
                  ************************************************************

HsCD00329521      GCAAGTCACATTCAAGAAAGCCGATTCTTATATCCTGGAAAGAATGGCAGACTTGGAGAC
NM_001516.4       GCAAGTCACATTCAAGAAAGCCGATTCTTATATCCTGGAAAGAATGGCAGACTTGGAGAC
                  ************************************************************

HsCD00329521      TTCTTCGGAGACCCTGGCAACCCTCCTGAATTTAATCCCTCTGGGAGTAAAGATGGAAAA
NM_001516.4       TTCTTCGGAGACCCTGGCAACCCTCCTGAATTTAATCCCTCTGGGAGTAAAGATGGAAAA
                  ************************************************************

HsCD00329521      TACGAACTTTTAACCTCAGCAAATGAAGTTATTGTTGAAGAGATTAAAGATCTAATGACC
NM_001516.4       TACGAACTTTTAACCTCAGCAAATGAAGTTATTGTTGAAGAGATTAAAGATCTAATGACC
                  ************************************************************

HsCD00329521      AAAAGTGACATAAAGGGTCAACATACAGAAACTTTGCTGGCAGGATCCCTGGCCAAAGCC
NM_001516.4       AAAAGTGACATAAAGGGTCAACATACAGAAACTTTGCTGGCAGGATCCCTGGCCAAAGCC
                  ************************************************************

HsCD00329521      CTTTGCTACATTCATAGAATGAACAAGGAAGTTAAAGACAATCAGGAAATGAAATCAAGG
NM_001516.4       CTTTGCTACATTCATAGAATGAACAAGGAAGTTAAAGACAATCAGGAAATGAAATCAAGG
                  ************************************************************

HsCD00329521      ATATTGGTGATTAAGGCTGCAGAAGACAGTGCGTTGCAGTATATGAACTTCATGAATGTC
NM_001516.4       ATATTGGTGATTAAGGCTGCAGAAGACAGTGCGTTGCAGTATATGAACTTCATGAATGTC
                  ************************************************************

HsCD00329521      ATCTTTGCAGCACAGAAACAGAATATTTTGATTGATGCCTGTGTTTTAGACTCCGACTCA
NM_001516.4       ATCTTTGCAGCACAGAAACAGAATATTTTGATTGATGCCTGTGTTTTAGACTCCGACTCA
                  ************************************************************

HsCD00329521      GGGCTCCTCCAACAGGCTTGTGACATCACGGGAGGACTGTACCTGAAGGTGCCTCAGATG
NM_001516.4       GGGCTCCTCCAACAGGCTTGTGACATCACGGGAGGACTGTACCTGAAGGTGCCTCAGATG
                  ************************************************************

HsCD00329521      CCTTCTCTTCTGCAGTATTTGCTGTGGGTGTTTCTTCCCGATCAAGATCAGAGATCTCAG
NM_001516.4       CCTTCTCTTCTGCAGTATTTGCTGTGGGTGTTTCTTCCCGATCAAGATCAGAGATCTCAG
                  ************************************************************

HsCD00329521      TTAATCCTCCCACCCCCGGTTCATGTTGACTACAGGGCTGCTTGCTTCTGTCATCGAAAT
NM_001516.4       TTAATCCTCCCACCCCCAGTTCATGTTGACTACAGGGCTGCTTGCTTCTGTCATCGAAAT
                  *****************.******************************************

HsCD00329521      CTCATTGAAATTGGTTATGTCTGTTCTGTGTGTTTGTCAATATTCTGCAATTTCAGCCCC
NM_001516.4       CTCATTGAAATTGGTTATGTCTGTTCTGTGTGTTTGTCAATATTCTGCAATTTCAGCCCC
                  ************************************************************

HsCD00329521      ATTTGTACTACGTGCGAGACAGCCTTTAAAATTTCTCTGCCTCCAGTGCTGAAAGCCAAG
NM_001516.4       ATTTGTACTACGTGCGAGACAGCCTTTAAAATTTCTCTGCCTCCAGTGCTGAAAGCCAAG
                  ************************************************************

HsCD00329521      AAAAAGAAACTGAAAGTGTCTGCCTTG
NM_001516.4       AAAAAGAAACTGAAAGTGTCTGCCTGA
                  ************************* .

Pathways

Panther : General transcription regulation
Panther : Transcription regulation by bZIP transcription factor
Reactome : DNA Repair
Reactome : Disease
Reactome : Dual incision reaction in GG-NER
Reactome : Dual incision reaction in TC-NER
Reactome : Epigenetic regulation of gene expression
Reactome : Formation of HIV elongation complex in the absence of HIV Tat
Reactome : Formation of HIV-1 elongation complex containing HIV-1 Tat
Reactome : Formation of RNA Pol II elongation complex
Reactome : Formation of incision complex in GG-NER
Reactome : Formation of the Early Elongation Complex
Reactome : Formation of the HIV-1 Early Elongation Complex
Reactome : Formation of transcription-coupled NER (TC-NER) repair complex
Reactome : Gene Expression
Reactome : Global Genomic NER (GG-NER)
Reactome : HIV Infection
Reactome : HIV Life Cycle
Reactome : HIV Transcription Elongation
Reactome : HIV Transcription Initiation
Reactome : Late Phase of HIV Life Cycle
Reactome : Negative epigenetic regulation of rRNA expression
Reactome : NoRC negatively regulates rRNA expression
Reactome : Nucleotide Excision Repair
Reactome : RNA Pol II CTD phosphorylation and interaction with CE
Reactome : RNA Polymerase I Chain Elongation
Reactome : RNA Polymerase I Promoter Clearance
Reactome : RNA Polymerase I Promoter Escape
Reactome : RNA Polymerase I Transcription
Reactome : RNA Polymerase I Transcription Initiation
Reactome : RNA Polymerase I Transcription Termination
Reactome : RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
Reactome : RNA Polymerase II HIV Promoter Escape
Reactome : RNA Polymerase II Pre-transcription Events
Reactome : RNA Polymerase II Promoter Escape
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Elongation
Reactome : RNA Polymerase II Transcription Initiation
Reactome : RNA Polymerase II Transcription Initiation And Promoter Clearance
Reactome : RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
Reactome : Tat-mediated elongation of the HIV-1 transcript
Reactome : Transcription
Reactome : Transcription of the HIV genome
Reactome : Transcription-coupled NER (TC-NER)
Reactome : mRNA Capping
WikiPathway : Eukaryotic Transcription Initiation

Protein

UniProt : Q13889
HPRD : 07531

Clone

Cloning Information : 268411
HIP Master Clone ID : 194908
Original Clone ID : FLH268411.01L

Organism


TAX_ID : 9606
Species Specific ID: 2967

Genome

Chromosome : 12
Map Location : 12q24.31
Ensembl : ENSG00000111358

Reagents

Labome : GTF2H3-antibody