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TAF1A (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TAF1A
Gene Name:  TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kDa
Sequence             
Original Clone ID: FLH269047.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329549

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1353nts         Open reading frame : 1 to 1353
Reference Sequence Alignment

ATGAGTGATTTCAGTGAAGAATTAAAAGGGCCTGTGACAGATGATGAAGAAGTGGAAACA
TCTGTGCTCAGTGGTGCAGGAATGCATTTTCCTTGGCTTCAAACATACGTAGAAACTGTG
GCCATTGGAGGGAAAAGGAGGAAGGATTTTGCTCAGACAACAAGTGCTTGTTTAAGTTTT
ATCCAAGAAGCTCTGCTGAAGCACCAATGGCAGCAAGCTGCAGAATACATGTACAGTTAT
TTTCAGACCTTGGAAGATTCAGATAGCTACAAAAGGCAGGCTGCACCTGAGATTATTTGG
AAGCTCGGAAGTGAAATTCTATTTTATCATCCCAAAAGCAACATGGAGAGTTTCAATACT
TTTGCTAACCGGATGAAAAATATTGGCGTCATGAATTATTTAAAGATCTCCTTACAACAT
GCATTATACCTTCTGCATCATGGAATGCTTAAAGATGCTAAGAGAAATCTGAGTGAGGCA
GAGACATGGAGACATGGTGAAAATACGTCTTCCCGGGAAATATTAATCAACCTTATTCAG
GCCTATAAAGGGCTTTTACAGTATTATACCTGGTCTGAAAAGAAGATGGAATTGTCAAAG
CTTGATAAGGATGATTATGCTTACAATGCAGTAGCCCAGGATGTGTTCAACCACAGCTGG
AAGACATCTGCAAATATTTCTGCATTGATTAAAATTCCTGGAGTTTGGGACCCTTTTGTG
AAGAGTTATGTAGAAATGCTGGAATTCTATGGGGATCGAGATGGAGCCCAAGAGGTACTC
ACCAATTATGCATATGATGAAAAGTTTCCATCAAATCCAAATGCCCATATCTACTTATAC
AACTTTCTAAAGAGACAGAAGGCACCAAGATCAAAATTGATAAGTGTGCTTAAGATTTTG
TATCAGATTGTACCATCTCATAAATTGATGTTGGAATTCCATACATTACTTAGAAAATCA
GAAAAAGAAGAACACCGTAAACTGGGGTTGGAGGTATTATTTGGAGTCTTAGATTTTGCC
GGATGCACTAAGAATATAACTGCTTGGAAATACTTGGCAAAATATCTGAAAAATATCTTA
ATGGGAAACCACCTTGCGTGGGTTCAAGAAGAGTGGAACTCCAGGAAAAACTGGTGGCCA
GGGTTTCATTTCAGCTACTTTTGGGCAAAAAGTGATTGGAAGGAAGATACAGCTTTGGCC
TGTGAGAAAGCTTTTGTGGCTGGTTTACTGTTAGGAAAAGGTTGTAGATATTTCCGGTAT
ATTTTAAAGCAAGATCACCAAATCTTAGGGAAGAAAATTAAGCGGATGAAGAGATCTGTG
AAAAAATACAGTATTGTAAATCCAAGACTCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1542
Start on reference sequence 190
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TAF1A
Symbol Nomenclature : TAF1A
SYNONYM : MGC:17061; RAFI48; SL1; TAFI48
Designation : RNA polymerase I-specific TBP-associated factor 48 kDa|SL1, 48kD subunit|TATA box-binding protein-associated factor 1A|TATA box-binding protein-associated factor RNA polymerase I subunit A|TBP-associated factor 1A|transcription factor SL1|transcription initiation factor SL1/TIF-IB subunit A
Full Nomenclature : TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kDa
GENEID : 9015
Locus Tag : RP11-378J18.2
HGNC : 11532
MIM : 604903
Vega : OTTHUMG00000037544
Target GenBank: BC013808

 

Reference Sequence Alignment

  

HsCD00329549        ATGAGTGATTTCAGTGAAGAATTAAAAGGGCCTGTGACAGATGATGAAGAAGTGGAAACA
NM_005681.3         ATGAGTGATTTCAGTGAAGAATTAAAAGGGCCTGTGACAGATGATGAAGAAGTGGAAACA
NM_001201536.1      ATGAGTGATTTCAGTGAAGAATTAAAAGGGCCTGTGACAGATGATGAAGAAGTGGAAACA
                    ************************************************************

HsCD00329549        TCTGTGCTCAGTGGTGCAGGAATGCATTTTCCTTGGCTTCAAACATACGTAGAAACTGTG
NM_005681.3         TCTGTGCTCAGTGGTGCAGGAATGCATTTTCCTTGGCTTCAAACATACGTAGAAACTGTG
NM_001201536.1      TCTGTGCTCAGTGGTGCAGGAATGCATTTTCCTTGGCTTCAAACATACGTAGAAACTGTG
                    ************************************************************

HsCD00329549        GCCATTGGAGGGAAAAGGAGGAAGGATTTTGCTCAGACAACAAGTGCTTGTTTAAGTTTT
NM_005681.3         GCCATTGGAGGGAAAAGGAGGAAGGATTTTGCTCAGACAACAAGTGCTTGTTTAAGTTTT
NM_001201536.1      GCCATTGGAGGGAAAAGGAGGAAGGATTTTGCTCAGACAACAAGTGCTTGTTTAAGTTTT
                    ************************************************************

HsCD00329549        ATCCAAGAAGCTCTGCTGAAGCACCAATGGCAGCAAGCTGCAGAATACATGTACAGTTAT
NM_005681.3         ATCCAAGAAGCTCTGCTGAAGCACCAATGGCAGCAAGCTGCAGAATACATGTACAGTTAT
NM_001201536.1      ATCCAAGAAGCTCTGCTGAAGCACCAATGGCAGCAAGCTGCAGAATACATGTACAGTTAT
                    ************************************************************

HsCD00329549        TTTCAGACCTTGGAAGATTCAGATAGCTACAAAAGGCAGGCTGCACCTGAGATTATTTGG
NM_005681.3         TTTCAGACCTTGGAAGATTCAGATAGCTACAAAAGGCAGGCTGCACCTGAGATTATTTGG
NM_001201536.1      TTTCAGACCTTGGAAGATTCAGATAGCTACAAAAGGCAGGCTGCACCTGAGATTATTTGG
                    ************************************************************

HsCD00329549        AAGCTCGGAAGTGAAATTCTATTTTATCATCCCAAAAGCAACATGGAGAGTTTCAATACT
NM_005681.3         AAGCTCGGAAGTGAAATTCTATTTTATCATCCCAAAAGCAACATGGAGAGTTTCAATACT
NM_001201536.1      AAGCTCGGAAGTGAAATTCTATTTTATCATCCCAAAAGCAACATGGAGAGTTTCAATACT
                    ************************************************************

HsCD00329549        TTTGCTAACCGGATGAAAAATATTGGCGTCATGAATTATTTAAAGATCTCCTTACAACAT
NM_005681.3         TTTGCTAACCGGATGAAAAATATTGGCGTCATGAATTATTTAAAGATCTCCTTACAACAT
NM_001201536.1      TTTGCTAACCGGATGAAAAATATTGGCGTCATGAATTATTTAAAGATCTCCTTACAACAT
                    ************************************************************

HsCD00329549        GCATTATACCTTCTGCATCATGGAATGCTTAAAGATGCTAAGAGAAATCTGAGTGAGGCA
NM_005681.3         GCATTATACCTTCTGCATCATGGAATGCTTAAAGATGCTAAGAGAAATCTGAGTGAGGCA
NM_001201536.1      GCATTATACCTTCTGCATCATGGAATGCTTAAAGATGCTAAGAGAAATCTGAGTGAGGCA
                    ************************************************************

HsCD00329549        GAGACATGGAGACATGGTGAAAATACGTCTTCCCGGGAAATATTAATCAACCTTATTCAG
NM_005681.3         GAGACATGGAGACATGGTGAAAATACGTCTTCCCGGGAAATATTAATCAACCTTATTCAG
NM_001201536.1      GAGACATGGAGACATGGTGAAAATACGTCTTCCCGGGAAATATTAATCAACCTTATTCAG
                    ************************************************************

HsCD00329549        GCCTATAAAGGGCTTTTACAGTATTATACCTGGTCTGAAAAGAAGATGGAATTGTCAAAG
NM_005681.3         GCCTATAAAGGGCTTTTACAGTATTATACCTGGTCTGAAAAGAAGATGGAATTGTCAAAG
NM_001201536.1      GCCTATAAAGGGCTTTTACAGTATTATACCTGGTCTGAAAAGAAGATGGAATTGTCAAAG
                    ************************************************************

HsCD00329549        CTTGATAAGGATGATTATGCTTACAATGCAGTAGCCCAGGATGTGTTCAACCACAGCTGG
NM_005681.3         CTTGATAAGGATGATTATGCTTACAATGCAGTAGCCCAGGATGTGTTCAACCACAGCTGG
NM_001201536.1      CTTGATAAGGATGATTATGCTTACAATGCAGTAGCCCAGGATGTGTTCAACCACAGCTGG
                    ************************************************************

HsCD00329549        AAGACATCTGCAAATATTTCTGCATTGATTAAAATTCCTGGAGTTTGGGACCCTTTTGTG
NM_005681.3         AAGACATCTGCAAATATTTCTGCATTGATTAAAATTCCTGGAGTTTGGGACCCTTTTGTG
NM_001201536.1      AAGACATCTGCAAATATTTCTGCATTGATTAAAATTCCTGGAGTTTGGGACCCTTTTGTG
                    ************************************************************

HsCD00329549        AAGAGTTATGTAGAAATGCTGGAATTCTATGGGGATCGAGATGGAGCCCAAGAGGTACTC
NM_005681.3         AAGAGTTATGTAGAAATGCTGGAATTCTATGGGGATCGAGATGGAGCCCAAGAGGTACTC
NM_001201536.1      AAGAGTTATGTAGAAATGCTGGAATTCTATGGGGATCGAGATGGAGCCCAAGAGGTACTC
                    ************************************************************

HsCD00329549        ACCAATTATGCATATGATGAAAAGTTTCCATCAAATCCAAATGCCCATATCTACTTATAC
NM_005681.3         ACCAATTATGCATATGATGAAAAGTTTCCATCAAATCCAAATGCCCATATCTACTTATAC
NM_001201536.1      ACCAATTATGCATATGATGAAAAGTTTCCATCAAATCCAAATGCCCATATCTACTTATAC
                    ************************************************************

HsCD00329549        AACTTTCTAAAGAGACAGAAGGCACCAAGATCAAAATTGATAAGTGTGCTTAAGATTTTG
NM_005681.3         AACTTTCTAAAGAGACAGAAGGCACCAAGATCAAAATTGATAAGTGTGCTTAAGATTTTG
NM_001201536.1      AACTTTCTAAAGAGACAGAAGGCACCAAGATCAAAATTGATAAGTGTGCTTAAGATTTTG
                    ************************************************************

HsCD00329549        TATCAGATTGTACCATCTCATAAATTGATGTTGGAATTCCATACATTACTTAGAAAATCA
NM_005681.3         TATCAGATTGTACCATCTCATAAATTGATGTTGGAATTCCATACATTACTTAGAAAATCA
NM_001201536.1      TATCAGATTGTACCATCTCATAAATTGATGTTGGAATTCCATACATTACTTAGAAAATCA
                    ************************************************************

HsCD00329549        GAAAAAGAAGAACACCGTAAACTGGGGTTGGAGGTATTATTTGGAGTCTTAGATTTTGCC
NM_005681.3         GAAAAAGAAGAACACCGTAAACTGGGGTTGGAGGTATTATTTGGAGTCTTAGATTTTGCC
NM_001201536.1      GAAAAAGAAGAACACCGTAAACTGGGGTTGGAGGTATTATTTGGAGTCTTAGATTTTGCC
                    ************************************************************

HsCD00329549        GGATGCACTAAGAATATAACTGCTTGGAAATACTTGGCAAAATATCTGAAAAATATCTTA
NM_005681.3         GGATGCACTAAGAATATAACTGCTTGGAAATACTTGGCAAAATATCTGAAAAATATCTTA
NM_001201536.1      GGATGCACTAAGAATATAACTGCTTGGAAATACTTGGCAAAATATCTGAAAAATATCTTA
                    ************************************************************

HsCD00329549        ATGGGAAACCACCTTGCGTGGGTTCAAGAAGAGTGGAACTCCAGGAAAAACTGGTGGCCA
NM_005681.3         ATGGGAAACCACCTTGCGTGGGTTCAAGAAGAGTGGAACTCCAGGAAAAACTGGTGGCCA
NM_001201536.1      ATGGGAAACCACCTTGCGTGGGTTCAAGAAGAGTGGAACTCCAGGAAAAACTGGTGGCCA
                    ************************************************************

HsCD00329549        GGGTTTCATTTCAGCTACTTTTGGGCAAAAAGTGATTGGAAGGAAGATACAGCTTTGGCC
NM_005681.3         GGCTTTCATTTCAGCTACTTTTGGGCAAAAAGTGATTGGAAGGAAGATACAGCTTTGGCC
NM_001201536.1      GGCTTTCATTTCAGCTACTTTTGGGCAAAAAGTGATTGGAAGGAAGATACAGCTTTGGCC
                    ** *********************************************************

HsCD00329549        TGTGAGAAAGCTTTTGTGGCTGGTTTACTGTTAGGAAAAGGTTGTAGATATTTCCGGTAT
NM_005681.3         TGTGAGAAAGCTTTTGTGGCTGGTTTACTGTTAGGAAAAGGTTGTAGATATTTCCGGTAT
NM_001201536.1      TGTGAGAAAGCTTTTGTGGCTGGTTTACTGTTAGGAAAAGGTTGTAGATATTTCCGGTAT
                    ************************************************************

HsCD00329549        ATTTTAAAGCAAGATCACCAAATCTTAGGGAAGAAAATTAAGCGGATGAAGAGATCTGTG
NM_005681.3         ATTTTAAAGCAAGATCACCAAATCTTAGGGAAGAAAATTAAGCGGATGAAGAGATCTGTG
NM_001201536.1      ATTTTAAAGCAAGATCACCAAATCTTAGGGAAGAAAATTAAGCGGATGAAGAGATCTGTG
                    ************************************************************

HsCD00329549        AAAAAATACAGTATTGTAAATCCAAGACTCTTG
NM_005681.3         AAAAAATACAGTATTGTAAATCCAAGACTCTGA
NM_001201536.1      AAAAAATACAGTATTGTAAATCCAAGACTCTGA
                    ******************************* .

Pathways

Panther : General transcription by RNA polymerase I
Reactome : Epigenetic regulation of gene expression
Reactome : Gene Expression
Reactome : Negative epigenetic regulation of rRNA expression
Reactome : NoRC negatively regulates rRNA expression
Reactome : RNA Polymerase I Chain Elongation
Reactome : RNA Polymerase I Promoter Clearance
Reactome : RNA Polymerase I Promoter Escape
Reactome : RNA Polymerase I Transcription
Reactome : RNA Polymerase I Transcription Initiation
Reactome : RNA Polymerase I Transcription Termination
Reactome : RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
Reactome : SIRT1 negatively regulates rRNA Expression
Reactome : Transcription

Protein

UniProt : A8K4K5
UniProt : B4DS21
UniProt : Q15573
HPRD : 05362

Clone

Cloning Information : 269047
HIP Master Clone ID : 142608
Original Clone ID : FLH269047.01L

Organism


TAX_ID : 9606
Species Specific ID: 9015

Genome

Chromosome : 1
Map Location : 1q42
Ensembl : ENSG00000143498

Reagents

Labome : TAF1A-antibody