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SUFU (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SUFU
Gene Name:  suppressor of fused homolog (Drosophila)
Sequence             
Original Clone ID: FLH269049.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329551

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1455nts         Open reading frame : 1 to 1455
Reference Sequence Alignment

ATGGCGGAGCTGCGGCCTAGCGGCGCCCCCGGCCCCACCGCGCCCCCGGCCCCTGGCCCG
ACTGCCCCCCCGGCCTTCGCTTCGCTCTTTCCCCCGGGACTGCACGCCATCTACGGAGAG
TGCCGCCGCCTTTACCCTGACCAGCCGAACCCGCTCCAGGTTACCGCTATCGTCAAGTAC
TGGTTGGGTGGCCCAGACCCCTTGGACTATGTTAGCATGTACAGGAATGTGGGGAGCCCT
TCTGCTAACATCCCCGAGCACTGGCACTACATCAGCTTCGGCCTGAGTGATCTCTATGGT
GACAACAGAGTCCATGAGTTTACAGGAACAGATGGACCTAGTGGTTTTGGCTTTGAGTTG
ACCTTTCGTCTGAAGAGAGAAACTGGGGAGTCTGCCCCACCAACATGGCCCGCAGAGTTA
ATGCAGGGCTTGGCACGATACGTGTTCCAGTCAGAGAACACCTTCTGCAGTGGGGACCAT
GTGTCCTGGCACAGCCCTTTGGATAACAGTGAGTCAAGAATTCAGCACATGCTGCTGACA
GAGGACCCACAGATGCAGCCCGTGCAGACACCCTTTGGGGTAGTTACCTTCCTCCAGATC
GTTGGTGTCTGCACTGAAGAGCTACACTCAGCCCAGCAGTGGAACGGGCAGGGCATCCTG
GAGCTGCTGCGGACAGTGCCTATTGCTGGCGGCCCCTGGCTGATAACTGACATGCGGAGG
GGAGAGACCATATTTGAGATCGATCCACACCTGCAAGAGAGAGTTGACAAAGGCATCGAG
ACAGATGGCTCCAACCTGAGTGGTGTCAGTGCCAAGTGTGCCTGGGATGACCTGAGCCGG
CCCCCCGAGGATGACGAGGACAGCCGGAGCATCTGCATCGGCACACAGCCCCGGCGACTC
TCTGGCAAAGACACAGAGCAGATCCGGGAGACCCTGAGGAGAGGACTCGAGATCAACAGC
AAACCTGTCCTTCCACCAATCAACCCTCAGCGGCAGAATGGCCTCGCCCACGACCGGGCC
CCGAGCCGCAAAGACAGCCTGGAAAGTGACAGCTCCACGGCCATCATTCCCCATGAGCTG
ATTCGCACGCGGCAGCTTGAGAGCGTACATCTGAAATTCAACCAGGAGTCCGGAGCCCTC
ATTCCTCTCTGCCTAAGGGGCAGGCTCCTGCATGGACGGCACTTTACATATAAAAGTATC
ACAGGTGACATGGCCATCACGTTTGTCTCCACGGGAGTGGAAGGCGCCTTTGCCACTGAG
GAGCATCCTTACGCGGCTCATGGACCCTGGTTACAAATTCTGTTGACCGAAGAGTTTGTA
GAGAAAATGTTGGAGGATTTAGAAGATTTGACTTCTCCAGAGGAATTCAAACTTCCCAAA
GAGTACAGCTGGCCTGAAAAGAAGCTGAAGGTCTCCATCCTGCCTGACGTGGTGTTCGAC
AGTCCGCTACACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1631
Start on reference sequence 177
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SUFU
Symbol Nomenclature : SUFU
SYNONYM : PRO1280; SUFUH; SUFUXL
Designation : suppressor of fused homolog
Full Nomenclature : suppressor of fused homolog (Drosophila)
GENEID : 51684
Locus Tag : RP11-47A8.1
HGNC : 16466
MIM : 607035
Vega : OTTHUMG00000018966
Target GenBank: BC013291

 

Reference Sequence Alignment

  

HsCD00329551      ATGGCGGAGCTGCGGCCTAGCGGCGCCCCCGGCCCCACCGCGCCCCCGGCCCCTGGCCCG
NM_016169.3       ATGGCGGAGCTGCGGCCTAGCGGCGCCCCCGGCCCCACCGCGCCCCCGGCCCCTGGCCCG
                  ************************************************************

HsCD00329551      ACTGCCCCCCCGGCCTTCGCTTCGCTCTTTCCCCCGGGACTGCACGCCATCTACGGAGAG
NM_016169.3       ACTGCCCCCCCGGCCTTCGCTTCGCTCTTTCCCCCGGGACTGCACGCCATCTACGGAGAG
                  ************************************************************

HsCD00329551      TGCCGCCGCCTTTACCCTGACCAGCCGAACCCGCTCCAGGTTACCGCTATCGTCAAGTAC
NM_016169.3       TGCCGCCGCCTTTACCCTGACCAGCCGAACCCGCTCCAGGTTACCGCTATCGTCAAGTAC
                  ************************************************************

HsCD00329551      TGGTTGGGTGGCCCAGACCCCTTGGACTATGTTAGCATGTACAGGAATGTGGGGAGCCCT
NM_016169.3       TGGTTGGGTGGCCCAGACCCCTTGGACTATGTTAGCATGTACAGGAATGTGGGGAGCCCT
                  ************************************************************

HsCD00329551      TCTGCTAACATCCCCGAGCACTGGCACTACATCAGCTTCGGCCTGAGTGATCTCTATGGT
NM_016169.3       TCTGCTAACATCCCCGAGCACTGGCACTACATCAGCTTCGGCCTGAGTGATCTCTATGGT
                  ************************************************************

HsCD00329551      GACAACAGAGTCCATGAGTTTACAGGAACAGATGGACCTAGTGGTTTTGGCTTTGAGTTG
NM_016169.3       GACAACAGAGTCCATGAGTTTACAGGAACAGATGGACCTAGTGGTTTTGGCTTTGAGTTG
                  ************************************************************

HsCD00329551      ACCTTTCGTCTGAAGAGAGAAACTGGGGAGTCTGCCCCACCAACATGGCCCGCAGAGTTA
NM_016169.3       ACCTTTCGTCTGAAGAGAGAAACTGGGGAGTCTGCCCCACCAACATGGCCCGCAGAGTTA
                  ************************************************************

HsCD00329551      ATGCAGGGCTTGGCACGATACGTGTTCCAGTCAGAGAACACCTTCTGCAGTGGGGACCAT
NM_016169.3       ATGCAGGGCTTGGCACGATACGTGTTCCAGTCAGAGAACACCTTCTGCAGTGGGGACCAT
                  ************************************************************

HsCD00329551      GTGTCCTGGCACAGCCCTTTGGATAACAGTGAGTCAAGAATTCAGCACATGCTGCTGACA
NM_016169.3       GTGTCCTGGCACAGCCCTTTGGATAACAGTGAGTCAAGAATTCAGCACATGCTGCTGACA
                  ************************************************************

HsCD00329551      GAGGACCCACAGATGCAGCCCGTGCAGACACCCTTTGGGGTAGTTACCTTCCTCCAGATC
NM_016169.3       GAGGACCCACAGATGCAGCCCGTGCAGACACCCTTTGGGGTAGTTACCTTCCTCCAGATC
                  ************************************************************

HsCD00329551      GTTGGTGTCTGCACTGAAGAGCTACACTCAGCCCAGCAGTGGAACGGGCAGGGCATCCTG
NM_016169.3       GTTGGTGTCTGCACTGAAGAGCTACACTCAGCCCAGCAGTGGAACGGGCAGGGCATCCTG
                  ************************************************************

HsCD00329551      GAGCTGCTGCGGACAGTGCCTATTGCTGGCGGCCCCTGGCTGATAACTGACATGCGGAGG
NM_016169.3       GAGCTGCTGCGGACAGTGCCTATTGCTGGCGGCCCCTGGCTGATAACTGACATGCGGAGG
                  ************************************************************

HsCD00329551      GGAGAGACCATATTTGAGATCGATCCACACCTGCAAGAGAGAGTTGACAAAGGCATCGAG
NM_016169.3       GGAGAGACCATATTTGAGATCGATCCACACCTGCAAGAGAGAGTTGACAAAGGCATCGAG
                  ************************************************************

HsCD00329551      ACAGATGGCTCCAACCTGAGTGGTGTCAGTGCCAAGTGTGCCTGGGATGACCTGAGCCGG
NM_016169.3       ACAGATGGCTCCAACCTGAGTGGTGTCAGTGCCAAGTGTGCCTGGGATGACCTGAGCCGG
                  ************************************************************

HsCD00329551      CCCCCCGAGGATGACGAGGACAGCCGGAGCATCTGCATCGGCACACAGCCCCGGCGACTC
NM_016169.3       CCCCCCGAGGATGACGAGGACAGCCGGAGCATCTGCATCGGCACACAGCCCCGGCGACTC
                  ************************************************************

HsCD00329551      TCTGGCAAAGACACAGAGCAGATCCGGGAGACCCTGAGGAGAGGACTCGAGATCAACAGC
NM_016169.3       TCTGGCAAAGACACAGAGCAGATCCGGGAGACCCTGAGGAGAGGACTCGAGATCAACAGC
                  ************************************************************

HsCD00329551      AAACCTGTCCTTCCACCAATCAACCCTCAGCGGCAGAATGGCCTCGCCCACGACCGGGCC
NM_016169.3       AAACCTGTCCTTCCACCAATCAACCCTCAGCGGCAGAATGGCCTCGCCCACGACCGGGCC
                  ************************************************************

HsCD00329551      CCGAGCCGCAAAGACAGCCTGGAAAGTGACAGCTCCACGGCCATCATTCCCCATGAGCTG
NM_016169.3       CCGAGCCGCAAAGACAGCCTGGAAAGTGACAGCTCCACGGCCATCATTCCCCATGAGCTG
                  ************************************************************

HsCD00329551      ATTCGCACGCGGCAGCTTGAGAGCGTACATCTGAAATTCAACCAGGAGTCCGGAGCCCTC
NM_016169.3       ATTCGCACGCGGCAGCTTGAGAGCGTACATCTGAAATTCAACCAGGAGTCCGGAGCCCTC
                  ************************************************************

HsCD00329551      ATTCCTCTCTGCCTAAGGGGCAGGCTCCTGCATGGACGGCACTTTACATATAAAAGTATC
NM_016169.3       ATTCCTCTCTGCCTAAGGGGCAGGCTCCTGCATGGACGGCACTTTACATATAAAAGTATC
                  ************************************************************

HsCD00329551      ACAGGTGACATGGCCATCACGTTTGTCTCCACGGGAGTGGAAGGCGCCTTTGCCACTGAG
NM_016169.3       ACAGGTGACATGGCCATCACGTTTGTCTCCACGGGAGTGGAAGGCGCCTTTGCCACTGAG
                  ************************************************************

HsCD00329551      GAGCATCCTTACGCGGCTCATGGACCCTGGTTACAAATTCTGTTGACCGAAGAGTTTGTA
NM_016169.3       GAGCATCCTTACGCGGCTCATGGACCCTGGTTACAAATTCTGTTGACCGAAGAGTTTGTA
                  ************************************************************

HsCD00329551      GAGAAAATGTTGGAGGATTTAGAAGATTTGACTTCTCCAGAGGAATTCAAACTTCCCAAA
NM_016169.3       GAGAAAATGTTGGAGGATTTAGAAGATTTGACTTCTCCAGAGGAATTCAAACTTCCCAAA
                  ************************************************************

HsCD00329551      GAGTACAGCTGGCCTGAAAAGAAGCTGAAGGTCTCCATCCTGCCTGACGTGGTGTTCGAC
NM_016169.3       GAGTACAGCTGGCCTGAAAAGAAGCTGAAGGTCTCCATCCTGCCTGACGTGGTGTTCGAC
                  ************************************************************

HsCD00329551      AGTCCGCTACACTTG
NM_016169.3       AGTCCGCTACACTAG
                  *************:*

Pathways

NCI : Hedgehog signaling events mediated by Gli proteins
Panther : Hedgehog signaling pathway
WikiPathway : Hedgehog Signaling Pathway

Protein

UniProt : Q9UMX1
HPRD : 06124

Clone

Cloning Information : 269049
HIP Master Clone ID : 162739
Original Clone ID : FLH269049.01L

Organism


TAX_ID : 9606
Species Specific ID: 51684

Genome

Chromosome : 10
Map Location : 10q24.32
Ensembl : ENSG00000107882

Reagents

Labome : SUFU-antibody