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SMYD3 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SMYD3
Gene Name:  SET and MYND domain containing 3
Sequence             
Original Clone ID: FLH269053.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329555

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1287nts         Open reading frame : 1 to 1287
Reference Sequence Alignment

ATGGAGCCGCTGAAGGTGGAAAAGTTCGCAACCGCCAACAGGGGAAACGGGCTGCGCGCC
GTGACCCCGCTGCGCCCCGGAGAGCTACTCTTCCGCTCGGATCCCTTGGCGTACACGGTG
TGCAAGGGGAGTCGTGGCGTCGTCTGCGACCGCTGCCTTCTCGGGAAGGAAAAGCTGATG
CGATGCTCTCAGTGCCGCGTCGCCAAATACTGTAGTGCTAAGTGTCAGAAAAAAGCTTGG
CCAGACCACAAGCGGGAATGCAAATGCCTTAAAAGCTGCAAACCCAGATATCCTCCAGAC
TCCGTTCGACTTCTTGGCAGAGTTGTCTTCAAACTTATGGATGGAGCACCTTCAGAATCA
GAGAAGCTTTACTCATTTTATGATCTGGAGTCAAATATTAACAAACTGACTGAAGATAGG
AAAGAGGGCCTCAGGCAACTCGTAATGACATTTCAACATTTCATGAGAGAAGAAATACAG
GATGCCTCTCAGCTGCCACCTGCCTTTGACCTTTTTGAAGCCTTTGCAAAAGTGATCTGC
AACTCTTTCACCATCTGTAATGCGGAGATGCAGGAAGTTGGTGTTGGCCTATATCCCAGT
ATCTCTTTGCTCAATCACAGCTGTGACCCCAACTGTTCGATTGTGTTCAATGGGCCCCAC
CTCTTACTGCGAGCAGTCCGAGACATCGAGGTGGGAGAGGAGCTCACCATCTGCTACCTG
GATATGCTGATGACCAGTGAGGAGCGCCGGAAGCAGCTGAGGGACCAGTACTGCTTTGAA
TGTGACTGTTTCCGTTGCCAAACCCAGGACAAGGATGCTGATATGCTAACTGGTGATGAG
CAAGTATGGAAGGAAGTTCAAGAATCCCTGAAAAAAATTGAAGAACTGAAGGCACACTGG
AAGTGGGAGCAGGTTCTGGCCATGTGCCAGGCGATCATAAGCAGCAATTCTGAACGGCTT
CCCGATATCAACATCTACCAGCTGAAGGTGCTCGACTGCGCCATGGATGCCTGCATCAAC
CTCGGCCTGTTGGAGGAAGCCTTGTTCTATGGTACTCGGACCATGGAGCCATACAGGATT
TTTTTCCCAGGAAGCCATCCCGTCAGAGGGGTTCAAGTGATGAAAGTTGGCAAACTGCAG
CTACATCAAGGCATGTTTCCCCAAGCAATGAAGAATCTGAGACTGGCTTTTGATATTATG
AGAGTGACACATGGCAGAGAACACAGCCTGATTGAAGATTTGATTCTACTTTTAGAAGAA
TGCGACGCCAACATCAGAGCATCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1327
Start on reference sequence 41
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SMYD3
Symbol Nomenclature : SMYD3
SYNONYM : KMT3E; ZMYND1; ZNFN3A1; bA74P14.1
Designation : SET and MYND domain-containing protein 3|bA74P14.1 (novel protein)|histone-lysine N-methyltransferase SMYD3|zinc finger MYND domain-containing protein 1|zinc finger protein, subfamily 3A (MYND domain containing), 1|zinc finger, MYND domain containing 1
Full Nomenclature : SET and MYND domain containing 3
GENEID : 64754
Locus Tag : RP11-49H10.1
HGNC : 15513
MIM : 608783
Vega : OTTHUMG00000040508
Target GenBank: BC031010

 

Reference Sequence Alignment

  

HsCD00329555        ATGGAGCCGCTGAAGGTGGAAAAGTTCGCAACCGCCAACAGGGGAAACGGGCTGCGCGCC
NM_001167740.1      ATGGAGCCGCTGAAGGTGGAAAAGTTCGCAACCGCCAAGAGGGGAAACGGGCTGCGCGCC
                    ************************************** *********************

HsCD00329555        GTGACCCCGCTGCGCCCCGGAGAGCTACTCTTCCGCTCGGATCCCTTGGCGTACACGGTG
NM_001167740.1      GTGACCCCGCTGCGCCCCGGAGAGCTACTCTTCCGCTCGGATCCCTTGGCGTACACGGTG
                    ************************************************************

HsCD00329555        TGCAAGGGGAGTCGTGGCGTCGTCTGCGACCGCTGCCTTCTCGGGAAGGAAAAGCTGATG
NM_001167740.1      TGCAAGGGGAGTCGTGGCGTCGTCTGCGACCGCTGCCTTCTCGGGAAGGAAAAGCTGATG
                    ************************************************************

HsCD00329555        CGATGCTCTCAGTGCCGCGTCGCCAAATACTGTAGTGCTAAGTGTCAGAAAAAAGCTTGG
NM_001167740.1      CGATGCTCTCAGTGCCGCGTCGCCAAATACTGTAGTGCTAAGTGTCAGAAAAAAGCTTGG
                    ************************************************************

HsCD00329555        CCAGACCACAAGCGGGAATGCAAATGCCTTAAAAGCTGCAAACCCAGATATCCTCCAGAC
NM_001167740.1      CCAGACCACAAGCGGGAATGCAAATGCCTTAAAAGCTGCAAACCCAGATATCCTCCAGAC
                    ************************************************************

HsCD00329555        TCCGTTCGACTTCTTGGCAGAGTTGTCTTCAAACTTATGGATGGAGCACCTTCAGAATCA
NM_001167740.1      TCCGTTCGACTTCTTGGCAGAGTTGTCTTCAAACTTATGGATGGAGCACCTTCAGAATCA
                    ************************************************************

HsCD00329555        GAGAAGCTTTACTCATTTTATGATCTGGAGTCAAATATTAACAAACTGACTGAAGATAGG
NM_001167740.1      GAGAAGCTTTACTCATTTTATGATCTGGAGTCAAATATTAACAAACTGACTGAAGATAAG
                    **********************************************************.*

HsCD00329555        AAAGAGGGCCTCAGGCAACTCGTAATGACATTTCAACATTTCATGAGAGAAGAAATACAG
NM_001167740.1      AAAGAGGGCCTCAGGCAACTCGTAATGACATTTCAACATTTCATGAGAGAAGAAATACAG
                    ************************************************************

HsCD00329555        GATGCCTCTCAGCTGCCACCTGCCTTTGACCTTTTTGAAGCCTTTGCAAAAGTGATCTGC
NM_001167740.1      GATGCCTCTCAGCTGCCACCTGCCTTTGACCTTTTTGAAGCCTTTGCAAAAGTGATCTGC
                    ************************************************************

HsCD00329555        AACTCTTTCACCATCTGTAATGCGGAGATGCAGGAAGTTGGTGTTGGCCTATATCCCAGT
NM_001167740.1      AACTCTTTCACCATCTGTAATGCGGAGATGCAGGAAGTTGGTGTTGGCCTATATCCCAGT
                    ************************************************************

HsCD00329555        ATCTCTTTGCTCAATCACAGCTGTGACCCCAACTGTTCGATTGTGTTCAATGGGCCCCAC
NM_001167740.1      ATCTCTTTGCTCAATCACAGCTGTGACCCCAACTGTTCGATTGTGTTCAATGGGCCCCAC
                    ************************************************************

HsCD00329555        CTCTTACTGCGAGCAGTCCGAGACATCGAGGTGGGAGAGGAGCTCACCATCTGCTACCTG
NM_001167740.1      CTCTTACTGCGAGCAGTCCGAGACATCGAGGTGGGAGAGGAGCTCACCATCTGCTACCTG
                    ************************************************************

HsCD00329555        GATATGCTGATGACCAGTGAGGAGCGCCGGAAGCAGCTGAGGGACCAGTACTGCTTTGAA
NM_001167740.1      GATATGCTGATGACCAGTGAGGAGCGCCGGAAGCAGCTGAGGGACCAGTACTGCTTTGAA
                    ************************************************************

HsCD00329555        TGTGACTGTTTCCGTTGCCAAACCCAGGACAAGGATGCTGATATGCTAACTGGTGATGAG
NM_001167740.1      TGTGACTGTTTCCGTTGCCAAACCCAGGACAAGGATGCTGATATGCTAACTGGTGATGAG
                    ************************************************************

HsCD00329555        CAAGTATGGAAGGAAGTTCAAGAATCCCTGAAAAAAATTGAAGAACTGAAGGCACACTGG
NM_001167740.1      CAAGTATGGAAGGAAGTTCAAGAATCCCTGAAAAAAATTGAAGAACTGAAGGCACACTGG
                    ************************************************************

HsCD00329555        AAGTGGGAGCAGGTTCTGGCCATGTGCCAGGCGATCATAAGCAGCAATTCTGAACGGCTT
NM_001167740.1      AAGTGGGAGCAGGTTCTGGCCATGTGCCAGGCAATCATAAGCAGCAATTCTGAACGGCTT
                    ********************************.***************************

HsCD00329555        CCCGATATCAACATCTACCAGCTGAAGGTGCTCGACTGCGCCATGGATGCCTGCATCAAC
NM_001167740.1      CCCGATATCAACATCTACCAGCTGAAGGTGCTCGACTGCGCCATGGATGCCTGCATCAAC
                    ************************************************************

HsCD00329555        CTCGGCCTGTTGGAGGAAGCCTTGTTCTATGGTACTCGGACCATGGAGCCATACAGGATT
NM_001167740.1      CTCGGCCTGTTGGAGGAAGCCTTGTTCTATGGTACTCGGACCATGGAGCCATACAGGATT
                    ************************************************************

HsCD00329555        TTTTTCCCAGGAAGCCATCCCGTCAGAGGGGTTCAAGTGATGAAAGTTGGCAAACTGCAG
NM_001167740.1      TTTTTCCCAGGAAGCCATCCCGTCAGAGGGGTTCAAGTGATGAAAGTTGGCAAACTGCAG
                    ************************************************************

HsCD00329555        CTACATCAAGGCATGTTTCCCCAAGCAATGAAGAATCTGAGACTGGCTTTTGATATTATG
NM_001167740.1      CTACATCAAGGCATGTTTCCCCAAGCAATGAAGAATCTGAGACTGGCTTTTGATATTATG
                    ************************************************************

HsCD00329555        AGAGTGACACATGGCAGAGAACACAGCCTGATTGAAGATTTGATTCTACTTTTAGAAGAA
NM_001167740.1      AGAGTGACACATGGCAGAGAACACAGCCTGATTGAAGATTTGATTCTACTTTTAGAAGAA
                    ************************************************************

HsCD00329555        TGCGACGCCAACATCAGAGCATCCTTG
NM_001167740.1      TGCGACGCCAACATCAGAGCATCCTAA
                    *************************:.

Clone

Cloning Information : 269053
HIP Master Clone ID : 162675
Original Clone ID : FLH269053.01L

Organism


TAX_ID : 9606
Species Specific ID: 64754

Genome

Chromosome : 1
Map Location : 1q44
Ensembl : ENSG00000185420

Reagents

Labome : SMYD3-antibody