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CTCF (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CTCF
Gene Name:  CCCTC-binding factor (zinc finger protein)
Sequence             
Original Clone ID: FLH269055.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329557

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2184nts         Open reading frame : 1 to 2184
Reference Sequence Alignment

ATGGAAGGTGATGCAGTCGAAGCCATTGTGGAGGAGTCCGAAACTTTTATTAAAGGAAAG
GAGAGAAAGACTTACCAGAGACGCCGGGAAGGGGGCCAGGAAGAAGATGCCTGCCACTTA
CCCCAGAACCAGACGGATGGGGGTGAGGTGGTCCAGGATGTCAACAGCAGTGTACAGATG
GTGATGATGGAACAGCTGGACCCCACCCTTCTTCAGATGAAGACTGAAGTAATGGAGGGC
ACAGTGGCTCCAGAAGCAGAGGCTGCTGTGGACGATACCCAGATTATAACTTTACAGGTT
GTAAATATGGAGGAACAGCCCATAAACATAGGAGAACTTCAGCTTGTTCAAGTACCTGTT
CCTGTGACTGTACCTGTTGCTACCACTTCAGTAGAAGAACTTCAGGGGGCTTATGAAAAT
GAAGTGTCTAAAGAGGGCCTTGCGGAAAGTGAACCCATGATATGCCACACCCTACCTTTG
CCTGAAGGGTTTCAGGTGGTTAAAGTGGGGGCCAATGGAGAGGTGGAGACACTAGAACAA
GGGGAACTTCCACCCCAGGAAGATCCTAGTTGGCAAAAAGACCCAGACTATCAGCCACCA
GCCAAAAAAACAAAGAAAACCAAAAAGAGCAAACTGCGTTATACAGAGGAGGGCAAAGAT
GTAGATGTGTCTGTCTACGATTTTGAGGAAGAACAGCAGGAGGGTCTGCTATCAGAGGTT
AATGCAGAGAAAGTGGTTGGTAATATGAAGCCTCCAAAGCCAACAAAAATTAAAAAGAAA
GGTGTAAAGAAGACATTCCAGTGTGAGCTTTGCAGTTACACGTGTCCACGGCGTTCAAAT
TTGGATCGTCACATGAAAAGCCACACTGATGAGAGACCACACAAGTGCCATCTCTGTGGC
AGGGCATTCAGAACAGTCACCCTCCTGAGGAATCACCTTAACACACACACAGGTACTCGT
CCTCACAAGTGCCCAGACTGCGACATGGCCTTTGTGACCAGTGGAGAATTGGTTCGGCAT
CGTCGTTACAAACACACCCACGAGAAGCCATTCAAGTGTTCCATGTGCGATTACGCCAGT
GTAGAAGTCAGCAAATTAAAACGTCACATTCGCTCTCATACTGGAGAGCGTCCGTTTCAG
TGCAGTTTGTGCAGTTATGCCAGCAGGGACACATACAAGCTGAAAAGGCACATGAGAACC
CATTCAGGGGAAAAGCCTTATGAATGTTATATTTGTCATGCTCGGTTTACCCAAAGTGGT
ACCATGAAGATGCACATTTTACAGAAGCACACAGAAAATGTGGCCAAATTTCACTGTCCC
CACTGTGACACAGTCATAGCCCGAAAAAGTGATTTGGGTGTCCACTTGCGAAAGCAGCAT
TCCTATATTGAGCAAGGCAAGAAATGCCGTTACTGTGATGCTGTGTTTCATGAGCGCTAT
GCCCTCATCCAGCATCAGAAGTCACACAAGAATGAGAAGCGCTTTAAGTGTGACCAGTGT
GATTACGCTTGTAGACAGGAGAGGCACATGATCATGCACAAGCGCACCCACACCGGGGAG
AAGCCTTACGCCTGCAGCCACTGCGATAAGACCTTCCGCCAGAAGCAGCTTCTCGACATG
CACTTCAAGCGCTATCACGACCCCAACTTCGTCCCTGCGGCTTTTGTCTGTTCTAAGTGT
GGGAAAACATTTACACGTCGGAATACCATGGCAAGACATGCTGATAATTGTGCTGGCCCA
GATGGCGTAGAGGGGGAAAATGGAGGAGAAACGAAGAAGAGTAAACGTGGAAGAAAAAGA
AAGATGCGCTCTAAGAAAGAAGATTCCTCTGACAGTGAAAATGCTGAACCAGATCTGGAC
GACAATGAGGATGAGGAGGAGCCTGCCGTAGAAATTGAACCTGAGCCAGAGCCTCAGCCT
GTGACCCCAGCCCCACCACCCGCCAAGAAGCGGAGAGGACGACCCCCTGGCAGAACCAAC
CAGCCCAAACAGAACCAGCCAACAGCTATCATTCAGGTTGAAGACCAGAATACAGGTGCA
ATTGAGAACATTATAGTTGAAGTAAAAAAAGAGCCAGATGCTGAGCCCGCAGAGGGAGAG
GAAGAGGAGGCCCAGCCAGCTGCCACAGATGCCCCCAACGGAGACCTCACGCCCGAGATG
ATCCTCAGCATGATGGACCGGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2471
Start on reference sequence 288
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CTCF
Symbol Nomenclature : CTCF
SYNONYM : MRD21
Designation : 11 zinc finger transcriptional repressor|11-zinc finger protein|CTCFL paralog|transcriptional repressor CTCF
Full Nomenclature : CCCTC-binding factor (zinc finger protein)
GENEID : 10664
HGNC : 13723
MIM : 604167
Vega : OTTHUMG00000137539
Target GenBank: BC014267

 

Reference Sequence Alignment

  

HsCD00329557      ATGGAAGGTGATGCAGTCGAAGCCATTGTGGAGGAGTCCGAAACTTTTATTAAAGGAAAG
NM_006565.3       ATGGAAGGTGATGCAGTCGAAGCCATTGTGGAGGAGTCCGAAACTTTTATTAAAGGAAAG
                  ************************************************************

HsCD00329557      GAGAGAAAGACTTACCAGAGACGCCGGGAAGGGGGCCAGGAAGAAGATGCCTGCCACTTA
NM_006565.3       GAGAGAAAGACTTACCAGAGACGCCGGGAAGGGGGCCAGGAAGAAGATGCCTGCCACTTA
                  ************************************************************

HsCD00329557      CCCCAGAACCAGACGGATGGGGGTGAGGTGGTCCAGGATGTCAACAGCAGTGTACAGATG
NM_006565.3       CCCCAGAACCAGACGGATGGGGGTGAGGTGGTCCAGGATGTCAACAGCAGTGTACAGATG
                  ************************************************************

HsCD00329557      GTGATGATGGAACAGCTGGACCCCACCCTTCTTCAGATGAAGACTGAAGTAATGGAGGGC
NM_006565.3       GTGATGATGGAACAGCTGGACCCCACCCTTCTTCAGATGAAGACTGAAGTAATGGAGGGC
                  ************************************************************

HsCD00329557      ACAGTGGCTCCAGAAGCAGAGGCTGCTGTGGACGATACCCAGATTATAACTTTACAGGTT
NM_006565.3       ACAGTGGCTCCAGAAGCAGAGGCTGCTGTGGACGATACCCAGATTATAACTTTACAGGTT
                  ************************************************************

HsCD00329557      GTAAATATGGAGGAACAGCCCATAAACATAGGAGAACTTCAGCTTGTTCAAGTACCTGTT
NM_006565.3       GTAAATATGGAGGAACAGCCCATAAACATAGGAGAACTTCAGCTTGTTCAAGTACCTGTT
                  ************************************************************

HsCD00329557      CCTGTGACTGTACCTGTTGCTACCACTTCAGTAGAAGAACTTCAGGGGGCTTATGAAAAT
NM_006565.3       CCTGTGACTGTACCTGTTGCTACCACTTCAGTAGAAGAACTTCAGGGGGCTTATGAAAAT
                  ************************************************************

HsCD00329557      GAAGTGTCTAAAGAGGGCCTTGCGGAAAGTGAACCCATGATATGCCACACCCTACCTTTG
NM_006565.3       GAAGTGTCTAAAGAGGGCCTTGCGGAAAGTGAACCCATGATATGCCACACCCTACCTTTG
                  ************************************************************

HsCD00329557      CCTGAAGGGTTTCAGGTGGTTAAAGTGGGGGCCAATGGAGAGGTGGAGACACTAGAACAA
NM_006565.3       CCTGAAGGGTTTCAGGTGGTTAAAGTGGGGGCCAATGGAGAGGTGGAGACACTAGAACAA
                  ************************************************************

HsCD00329557      GGGGAACTTCCACCCCAGGAAGATCCTAGTTGGCAAAAAGACCCAGACTATCAGCCACCA
NM_006565.3       GGGGAACTTCCACCCCAGGAAGATCCTAGTTGGCAAAAAGACCCAGACTATCAGCCACCA
                  ************************************************************

HsCD00329557      GCCAAAAAAACAAAGAAAACCAAAAAGAGCAAACTGCGTTATACAGAGGAGGGCAAAGAT
NM_006565.3       GCCAAAAAAACAAAGAAAACCAAAAAGAGCAAACTGCGTTATACAGAGGAGGGCAAAGAT
                  ************************************************************

HsCD00329557      GTAGATGTGTCTGTCTACGATTTTGAGGAAGAACAGCAGGAGGGTCTGCTATCAGAGGTT
NM_006565.3       GTAGATGTGTCTGTCTACGATTTTGAGGAAGAACAGCAGGAGGGTCTGCTATCAGAGGTT
                  ************************************************************

HsCD00329557      AATGCAGAGAAAGTGGTTGGTAATATGAAGCCTCCAAAGCCAACAAAAATTAAAAAGAAA
NM_006565.3       AATGCAGAGAAAGTGGTTGGTAATATGAAGCCTCCAAAGCCAACAAAAATTAAAAAGAAA
                  ************************************************************

HsCD00329557      GGTGTAAAGAAGACATTCCAGTGTGAGCTTTGCAGTTACACGTGTCCACGGCGTTCAAAT
NM_006565.3       GGTGTAAAGAAGACATTCCAGTGTGAGCTTTGCAGTTACACGTGTCCACGGCGTTCAAAT
                  ************************************************************

HsCD00329557      TTGGATCGTCACATGAAAAGCCACACTGATGAGAGACCACACAAGTGCCATCTCTGTGGC
NM_006565.3       TTGGATCGTCACATGAAAAGCCACACTGATGAGAGACCACACAAGTGCCATCTCTGTGGC
                  ************************************************************

HsCD00329557      AGGGCATTCAGAACAGTCACCCTCCTGAGGAATCACCTTAACACACACACAGGTACTCGT
NM_006565.3       AGGGCATTCAGAACAGTCACCCTCCTGAGGAATCACCTTAACACACACACAGGTACTCGT
                  ************************************************************

HsCD00329557      CCTCACAAGTGCCCAGACTGCGACATGGCCTTTGTGACCAGTGGAGAATTGGTTCGGCAT
NM_006565.3       CCTCACAAGTGCCCAGACTGCGACATGGCCTTTGTGACCAGTGGAGAATTGGTTCGGCAT
                  ************************************************************

HsCD00329557      CGTCGTTACAAACACACCCACGAGAAGCCATTCAAGTGTTCCATGTGCGATTACGCCAGT
NM_006565.3       CGTCGTTACAAACACACCCACGAGAAGCCATTCAAGTGTTCCATGTGCGATTACGCCAGT
                  ************************************************************

HsCD00329557      GTAGAAGTCAGCAAATTAAAACGTCACATTCGCTCTCATACTGGAGAGCGTCCGTTTCAG
NM_006565.3       GTAGAAGTCAGCAAATTAAAACGTCACATTCGCTCTCATACTGGAGAGCGTCCGTTTCAG
                  ************************************************************

HsCD00329557      TGCAGTTTGTGCAGTTATGCCAGCAGGGACACATACAAGCTGAAAAGGCACATGAGAACC
NM_006565.3       TGCAGTTTGTGCAGTTATGCCAGCAGGGACACATACAAGCTGAAAAGGCACATGAGAACC
                  ************************************************************

HsCD00329557      CATTCAGGGGAAAAGCCTTATGAATGTTATATTTGTCATGCTCGGTTTACCCAAAGTGGT
NM_006565.3       CATTCAGGGGAAAAGCCTTATGAATGTTATATTTGTCATGCTCGGTTTACCCAAAGTGGT
                  ************************************************************

HsCD00329557      ACCATGAAGATGCACATTTTACAGAAGCACACAGAAAATGTGGCCAAATTTCACTGTCCC
NM_006565.3       ACCATGAAGATGCACATTTTACAGAAGCACACAGAAAATGTGGCCAAATTTCACTGTCCC
                  ************************************************************

HsCD00329557      CACTGTGACACAGTCATAGCCCGAAAAAGTGATTTGGGTGTCCACTTGCGAAAGCAGCAT
NM_006565.3       CACTGTGACACAGTCATAGCCCGAAAAAGTGATTTGGGTGTCCACTTGCGAAAGCAGCAT
                  ************************************************************

HsCD00329557      TCCTATATTGAGCAAGGCAAGAAATGCCGTTACTGTGATGCTGTGTTTCATGAGCGCTAT
NM_006565.3       TCCTATATTGAGCAAGGCAAGAAATGCCGTTACTGTGATGCTGTGTTTCATGAGCGCTAT
                  ************************************************************

HsCD00329557      GCCCTCATCCAGCATCAGAAGTCACACAAGAATGAGAAGCGCTTTAAGTGTGACCAGTGT
NM_006565.3       GCCCTCATCCAGCATCAGAAGTCACACAAGAATGAGAAGCGCTTTAAGTGTGACCAGTGT
                  ************************************************************

HsCD00329557      GATTACGCTTGTAGACAGGAGAGGCACATGATCATGCACAAGCGCACCCACACCGGGGAG
NM_006565.3       GATTACGCTTGTAGACAGGAGAGGCACATGATCATGCACAAGCGCACCCACACCGGGGAG
                  ************************************************************

HsCD00329557      AAGCCTTACGCCTGCAGCCACTGCGATAAGACCTTCCGCCAGAAGCAGCTTCTCGACATG
NM_006565.3       AAGCCTTACGCCTGCAGCCACTGCGATAAGACCTTCCGCCAGAAGCAGCTTCTCGACATG
                  ************************************************************

HsCD00329557      CACTTCAAGCGCTATCACGACCCCAACTTCGTCCCTGCGGCTTTTGTCTGTTCTAAGTGT
NM_006565.3       CACTTCAAGCGCTATCACGACCCCAACTTCGTCCCTGCGGCTTTTGTCTGTTCTAAGTGT
                  ************************************************************

HsCD00329557      GGGAAAACATTTACACGTCGGAATACCATGGCAAGACATGCTGATAATTGTGCTGGCCCA
NM_006565.3       GGGAAAACATTTACACGTCGGAATACCATGGCAAGACATGCTGATAATTGTGCTGGCCCA
                  ************************************************************

HsCD00329557      GATGGCGTAGAGGGGGAAAATGGAGGAGAAACGAAGAAGAGTAAACGTGGAAGAAAAAGA
NM_006565.3       GATGGCGTAGAGGGGGAAAATGGAGGAGAAACGAAGAAGAGTAAACGTGGAAGAAAAAGA
                  ************************************************************

HsCD00329557      AAGATGCGCTCTAAGAAAGAAGATTCCTCTGACAGTGAAAATGCTGAACCAGATCTGGAC
NM_006565.3       AAGATGCGCTCTAAGAAAGAAGATTCCTCTGACAGTGAAAATGCTGAACCAGATCTGGAC
                  ************************************************************

HsCD00329557      GACAATGAGGATGAGGAGGAGCCTGCCGTAGAAATTGAACCTGAGCCAGAGCCTCAGCCT
NM_006565.3       GACAATGAGGATGAGGAGGAGCCTGCCGTAGAAATTGAACCTGAGCCAGAGCCTCAGCCT
                  ************************************************************

HsCD00329557      GTGACCCCAGCCCCACCACCCGCCAAGAAGCGGAGAGGACGACCCCCTGGCAGAACCAAC
NM_006565.3       GTGACCCCAGCCCCACCACCCGCCAAGAAGCGGAGAGGACGACCCCCTGGCAGAACCAAC
                  ************************************************************

HsCD00329557      CAGCCCAAACAGAACCAGCCAACAGCTATCATTCAGGTTGAAGACCAGAATACAGGTGCA
NM_006565.3       CAGCCCAAACAGAACCAGCCAACAGCTATCATTCAGGTTGAAGACCAGAATACAGGTGCA
                  ************************************************************

HsCD00329557      ATTGAGAACATTATAGTTGAAGTAAAAAAAGAGCCAGATGCTGAGCCCGCAGAGGGAGAG
NM_006565.3       ATTGAGAACATTATAGTTGAAGTAAAAAAAGAGCCAGATGCTGAGCCCGCAGAGGGAGAG
                  ************************************************************

HsCD00329557      GAAGAGGAGGCCCAGCCAGCTGCCACAGATGCCCCCAACGGAGACCTCACGCCCGAGATG
NM_006565.3       GAAGAGGAGGCCCAGCCAGCTGCCACAGATGCCCCCAACGGAGACCTCACGCCCGAGATG
                  ************************************************************

HsCD00329557      ATCCTCAGCATGATGGACCGGTTG
NM_006565.3       ATCCTCAGCATGATGGACCGGTGA
                  ********************** .

Pathways

WikiPathway : SIDS Susceptibility Pathways

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : B5MC38
UniProt : P49711
UniProt : Q59EL8
HPRD : 05005

Clone

Cloning Information : 269055
HIP Master Clone ID : 163051
Original Clone ID : FLH269055.01L

Organism


TAX_ID : 9606
Species Specific ID: 10664

Genome

Chromosome : 16
Map Location : 16q21-q22.3
Ensembl : ENSG00000102974

Reagents

Labome : CTCF-antibody