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PYGO2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PYGO2
Gene Name:  pygopus family PHD finger 2
Sequence             
Original Clone ID: FLH269065.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329566

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1221nts         Open reading frame : 1 to 1221
Reference Sequence Alignment

ATGGCCGCCTCGGCGCCGCCCCCACCGGACAAGCTGGAGGGAGGTGGCGGCCCCGCACCG
CCCCCTGCGCCGCCCAGCACCGGGAGGAAGCAGGGCAAGGCCGGTCTGCAAATGAAGAGT
CCAGAAAAGAAGCGAAGGAAGTCAAATACTCAGGGCCCTGCATACTCACATCTGACGGAG
TTTGCACCACCCCCAACTCCCATGGTGGATCACCTGGTTGCATCCAACCCTTTTGAAGAT
GACTTCGGAGCCCCCAAAGTGGGGGTTGCAGCCCCTCCATTCCTTGGCAGTCCTGTGCCC
TTCGGAGGCTTCCGTGTGCAGGGGGGCATGGCGGGCCAGGTACCCCCAGGCTACAGCACT
GGAGGTGGAGGGGGCCCCCAGCCACTCCGTCGACAGCCACCCCCCTTCCCTCCCAATCCT
ATGGGCCCTGCTTTCAACATGCCCCCCCAGGGTCCTGGCTACCCACCCCCAGGCAACATG
AACTTTCCCAGCCAACCCTTCAACCAGCCTCTGGGTCAAAACTTTAGTCCTCCCAGTGGG
CAGATGATGCCGGGCCCAGTGGGGGGATTTGGTCCCATGATCTCACCCACCATGGGACAG
CCTCCCAGAGCAGAGCTGGGCCCACCTTCTCTGTCCCAACGATTTGCTCAGCCAGGGGCT
CCTTTTGGCCCTTCTCCTCTCCAGAGACCTGGTCAGGGGCTCCCCAGCCTGCCGCCTAAC
ACAAGTCCCTTTCCTGGTCCGGACCCTGGCTTTCCTGGCCCTGGTGGTGAGGATGGGGGG
AAGCCCTTGAATCCACCTGCTTCTACTGCTTTTCCCCAGGAGCCCCACTCAGGCTCCCCG
GCTGCTGCTGTTAATGGGAACCAGCCCAGTTTCCCCCCGAACAGCAGTGGGCGGGGTGGG
GGCACTCCAGATGCCAACAGCTTGGCACCCCCTGGCAAGGCAGGTGGGGGCTCCGGGCCC
CAGCCTCCCCCAGGCTTGGTGTACCCATGTGGTGCCTGTCGGAGTGAGGTGAACGATGAC
CAGGATGCCATTCTGTGTGAGGCCTCCTGCCAGAAATGGTTCCACCGTGAGTGCACAGGC
ATGACTGAGAGCGCCTATGGGCTGCTGACCACTGAAGCTTCTGCCGTCTGGGCCTGCGAT
CTCTGCCTCAAGACCAAGGAGATCCAGTCTGTCTACATCCGTGAGGGCATGGGGCAGCTG
GTGGCTGCTAACGATGGGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1393
Start on reference sequence 173
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PYGO2
Symbol Nomenclature : PYGO2
SYNONYM : 1190004M21Rik
Designation : pygopus 2|pygopus homolog 2
Full Nomenclature : pygopus family PHD finger 2
GENEID : 90780
Locus Tag : PP7910
HGNC : 30257
MIM : 606903
Vega : OTTHUMG00000037370
Target GenBank: BC032099

 

Reference Sequence Alignment

  

HsCD00329566      ATGGCCGCCTCGGCGCCGCCCCCACCGGACAAGCTGGAGGGAGGTGGCGGCCCCGCACCG
NM_138300.3       ATGGCCGCCTCGGCGCCGCCCCCACCGGACAAGCTGGAGGGAGGTGGCGGCCCCGCACCG
                  ************************************************************

HsCD00329566      CCCCCTGCGCCGCCCAGCACCGGGAGGAAGCAGGGCAAGGCCGGTCTGCAAATGAAGAGT
NM_138300.3       CCCCCTGCGCCGCCCAGCACCGGGAGGAAGCAGGGCAAGGCCGGTCTGCAAATGAAGAGT
                  ************************************************************

HsCD00329566      CCAGAAAAGAAGCGAAGGAAGTCAAATACTCAGGGCCCTGCATACTCACATCTGACGGAG
NM_138300.3       CCAGAAAAGAAGCGAAGGAAGTCAAATACTCAGGGCCCTGCATACTCACATCTGACGGAG
                  ************************************************************

HsCD00329566      TTTGCACCACCCCCAACTCCCATGGTGGATCACCTGGTTGCATCCAACCCTTTTGAAGAT
NM_138300.3       TTTGCACCACCCCCAACTCCCATGGTGGATCACCTGGTTGCATCCAACCCTTTTGAAGAT
                  ************************************************************

HsCD00329566      GACTTCGGAGCCCCCAAAGTGGGGGTTGCAGCCCCTCCATTCCTTGGCAGTCCTGTGCCC
NM_138300.3       GACTTCGGAGCCCCCAAAGTGGGGGTTGCAGCCCCTCCATTCCTTGGCAGTCCTGTGCCC
                  ************************************************************

HsCD00329566      TTCGGAGGCTTCCGTGTGCAGGGGGGCATGGCGGGCCAGGTACCCCCAGGCTACAGCACT
NM_138300.3       TTCGGAGGCTTCCGTGTGCAGGGGGGCATGGCGGGCCAGGTACCCCCAGGCTACAGCACT
                  ************************************************************

HsCD00329566      GGAGGTGGAGGGGGCCCCCAGCCACTCCGTCGACAGCCACCCCCCTTCCCTCCCAATCCT
NM_138300.3       GGAGGTGGAGGGGGCCCCCAGCCACTCCGTCGACAGCCACCCCCCTTCCCTCCCAATCCT
                  ************************************************************

HsCD00329566      ATGGGCCCTGCTTTCAACATGCCCCCCCAGGGTCCTGGCTACCCACCCCCAGGCAACATG
NM_138300.3       ATGGGCCCTGCTTTCAACATGCCCCCCCAGGGTCCTGGCTACCCACCCCCAGGCAACATG
                  ************************************************************

HsCD00329566      AACTTTCCCAGCCAACCCTTCAACCAGCCTCTGGGTCAAAACTTTAGTCCTCCCAGTGGG
NM_138300.3       AACTTTCCCAGCCAACCCTTCAACCAGCCTCTGGGTCAAAACTTTAGTCCTCCCAGTGGG
                  ************************************************************

HsCD00329566      CAGATGATGCCGGGCCCAGTGGGGGGATTTGGTCCCATGATCTCACCCACCATGGGACAG
NM_138300.3       CAGATGATGCCGGGCCCAGTGGGGGGATTTGGTCCCATGATCTCACCCACCATGGGACAG
                  ************************************************************

HsCD00329566      CCTCCCAGAGCAGAGCTGGGCCCACCTTCTCTGTCCCAACGATTTGCTCAGCCAGGGGCT
NM_138300.3       CCTCCCAGAGCAGAGCTGGGCCCACCTTCTCTGTCCCAACGATTTGCTCAGCCAGGGGCT
                  ************************************************************

HsCD00329566      CCTTTTGGCCCTTCTCCTCTCCAGAGACCTGGTCAGGGGCTCCCCAGCCTGCCGCCTAAC
NM_138300.3       CCTTTTGGCCCTTCTCCTCTCCAGAGACCTGGTCAGGGGCTCCCCAGCCTGCCGCCTAAC
                  ************************************************************

HsCD00329566      ACAAGTCCCTTTCCTGGTCCGGACCCTGGCTTTCCTGGCCCTGGTGGTGAGGATGGGGGG
NM_138300.3       ACAAGTCCCTTTCCTGGTCCGGACCCTGGCTTTCCTGGCCCTGGTGGTGAGGATGGGGGG
                  ************************************************************

HsCD00329566      AAGCCCTTGAATCCACCTGCTTCTACTGCTTTTCCCCAGGAGCCCCACTCAGGCTCCCCG
NM_138300.3       AAGCCCTTGAATCCACCTGCTTCTACTGCTTTTCCCCAGGAGCCCCACTCAGGCTCCCCG
                  ************************************************************

HsCD00329566      GCTGCTGCTGTTAATGGGAACCAGCCCAGTTTCCCCCCGAACAGCAGTGGGCGGGGTGGG
NM_138300.3       GCTGCTGCTGTTAATGGGAACCAGCCCAGTTTCCCCCCGAACAGCAGTGGGCGGGGTGGG
                  ************************************************************

HsCD00329566      GGCACTCCAGATGCCAACAGCTTGGCACCCCCTGGCAAGGCAGGTGGGGGCTCCGGGCCC
NM_138300.3       GGCACTCCAGATGCCAACAGCTTGGCACCCCCTGGCAAGGCAGGTGGGGGCTCCGGGCCC
                  ************************************************************

HsCD00329566      CAGCCTCCCCCAGGCTTGGTGTACCCATGTGGTGCCTGTCGGAGTGAGGTGAACGATGAC
NM_138300.3       CAGCCTCCCCCAGGCTTGGTGTACCCATGTGGTGCCTGTCGGAGTGAGGTGAACGATGAC
                  ************************************************************

HsCD00329566      CAGGATGCCATTCTGTGTGAGGCCTCCTGCCAGAAATGGTTCCACCGTGAGTGCACAGGC
NM_138300.3       CAGGATGCCATTCTGTGTGAGGCCTCCTGCCAGAAATGGTTCCACCGTGAGTGCACAGGC
                  ************************************************************

HsCD00329566      ATGACTGAGAGCGCCTATGGGCTGCTGACCACTGAAGCTTCTGCCGTCTGGGCCTGCGAT
NM_138300.3       ATGACTGAGAGCGCCTATGGGCTGCTGACCACTGAAGCTTCTGCCGTCTGGGCCTGCGAT
                  ************************************************************

HsCD00329566      CTCTGCCTCAAGACCAAGGAGATCCAGTCTGTCTACATCCGTGAGGGCATGGGGCAGCTG
NM_138300.3       CTCTGCCTCAAGACCAAGGAGATCCAGTCTGTCTACATCCGTGAGGGCATGGGGCAGCTG
                  ************************************************************

HsCD00329566      GTGGCTGCTAACGATGGGTTG
NM_138300.3       GTGGCTGCTAACGATGGGTGA
                  ******************* .

Pathways

Panther : Wnt signaling pathway
Reactome : Signal Transduction
Reactome : Signaling by Wnt
Reactome : TCF dependent signaling in response to WNT
Reactome : deactivation of the beta-catenin transactivating complex
Reactome : formation of the beta-catenin:TCF transactivating complex

Protein

SMART domain : PHD : PHD zinc finger
UniProt : Q9BRQ0
UniProt : Q5T170
HPRD : 06065

Clone

Cloning Information : 269065
HIP Master Clone ID : 174068
Original Clone ID : FLH269065.01L

Organism


TAX_ID : 9606
Species Specific ID: 90780

Genome

Chromosome : 1
Map Location : 1q21.3
Ensembl : ENSG00000163348

Reagents

Labome : PYGO2-antibody