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ZNF496 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF496
Gene Name:  zinc finger protein 496
Sequence             
Original Clone ID: FLH269093.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329591

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1764nts         Open reading frame : 1 to 1764
Reference Sequence Alignment

ATGCCCACAGCCCTGTGCCCCCGAGTCTTGGCTCCGAAGGAAAGTGAGGAGCCCAGGAAA
ATGAGGAGCCCACCTGGAGAGAACCCTAGCCCCCAGGGGGAGCTTCCCAGCCCCGAGTCC
TCTCGGCGTCTCTTCCGCCGTTTCCGCTACCAGGAGGCGGCGGGCCCCCGGGAGGCCCTG
CAGCGCCTCTGGGACCTGTGCGGGGGCTGGCTGCGGCCTGAGAGGCACACCAAGGAGCAG
ATTCTGGAGCTGCTGGTGCTGGAGCAGTTCCTGGCCATCCTGCCCCGGGAGATCCAGAGC
TGGGTGCGGGCGCAGGAGCCTGAGAGCGGAGAGCAGGCTGTGGCCGCGGTGGAGGCACTG
GAACGGGAGCCCGGGAGACCCTGGCAGTGGCTCAAGCACTGTGAAGACCCTGTGGTGATT
GATGATGGGGACAGCCCTCTGGACCAGGAGCAGGAGCAGCTGCCGGTAGAGCCCCACAGC
GACCTTGCAAAGAACCAGGATGCCCAGCCCATAACCCTGGCACAGTGCCTGGGGCTCCCA
AGCAGACCACCAAGCCAGCTCAGCGGGGACCCAGTTCTGCAAGATGCTTTCCTTCTTCAG
GAAGAGAATGTGCGGGACACACAGCAGGTGACCACCCTCCAGCTACCCCCGAGTCGGGTT
TCTCCATTCAAGGACATGATTTTATGCTTCTCTGAAGAGGATTGGTCCCTTCTAGATCCT
GCCCAGACTGGCTTCTATGGAGAGTTCATCATTGGGGAGGATTACGGGGTCTCAATGCCT
CCAAACGACCTAGCTGCCCAGCCAGATCTCTCCCAGGGAGAGGAGAATGAGCCACGCGTT
CCAGAGTTGCAGGATCTCCAGGGCAAAGAAGTGCCCCAGGTCTCCTACTTGGACTCTCCA
AGTCTCCAGCCATTCCAGGTAGAAGAAAGAAGGAAACGTGAGGAGCTGCAGGTGCCAGAG
TTCCAGGCCTGCCCACAGACGGTGGTGCCTCAAAACACCTACCCAGCTGGCGGCAACCCG
CGATCTCTAGAGAACAGCCTGGATGAAGAAGTGACCATCGAGATCGTTCTCTCCAGCTCT
GGGGACGAGGACTCCCAGCATGGCCCGTACTGCACAGAAGAGCTGGGGAGCCCCACAGAG
AAGCAGCGCAGCCTCCCCGCCTCCCACCGGAGCAGCACCGAGGCCGGAGGCGAGGTGCAG
ACCTCCAAGAAGTCCTACGTGTGTCCGAACTGTGGGAAAATCTTCCGCTGGAGGGTCAAC
TTCATCCGGCATCTGCGGAGCCGCAGGGAGCAGGAGAAGCCGCACGAGTGCTCGGTGTGC
GGGGAGCTGTTCAGCGACAGCGAGGACCTGGATGGGCACCTAGAGAGCCACGAGGCCCAG
AAGCCTTACCGGTGTGGTGCCTGCGGGAAGAGCTTCCGCCTGAACTCCCACCTGCTCTCC
CACCGGCGGATACACCTGCAGCCGGACAGACTCCAGCCGGTGGAGAAGAGAGAGCAGGCG
GCATCCGAGGACGCGGACAAGGGTCCCAAAGAGCCGCTGGAAAACGGCAAGGCCAAACTG
AGCTTCCAGTGCTGTGAGTGTGGGAAGGCCTTCCAGCGGCACGACCACCTGGCTCGGCAC
CGCAGCCACTTTCACCTGAAGGACAAAGCCCGGCCCTTCCAGTGCCGGTACTGCGTCAAG
AGCTTCACGCAGAACTATGACCTCCTCCGCCACGAGCGCCTGCACATGAAGCGCCGTTCC
AAGCAGGCTCTGAACTCCTACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2229
Start on reference sequence 466
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF496
Symbol Nomenclature : ZNF496
SYNONYM : NIZP1; ZFP496; ZKSCAN17; ZSCAN49
Designation : NSD1 (nuclear receptor binding SET-domain containing 1)-interacting zinc finger protein 1|zinc finger protein with KRAB and SCAN domains 17
Full Nomenclature : zinc finger protein 496
GENEID : 84838
HGNC : 23713
MIM : 613911
Vega : OTTHUMG00000041164
Target GenBank: BC007263

 

Reference Sequence Alignment

  

HsCD00329591      ATGCCCACAGCCCTGTGCCCCCGAGTCTTGGCTCCGAAGGAAAGTGAGGAGCCCAGGAAA
NM_032752.1       ATGCCCACAGCCCTGTGCCCCCGAGTCTTGGCTCCGAAGGAAAGTGAGGAGCCCAGGAAA
                  ************************************************************

HsCD00329591      ATGAGGAGCCCACCTGGAGAGAACCCTAGCCCCCAGGGGGAGCTTCCCAGCCCCGAGTCC
NM_032752.1       ATGAGGAGCCCACCTGGAGAGAACCCTAGCCCCCAGGGGGAGCTTCCCAGCCCCGAGTCC
                  ************************************************************

HsCD00329591      TCTCGGCGTCTCTTCCGCCGTTTCCGCTACCAGGAGGCGGCGGGCCCCCGGGAGGCCCTG
NM_032752.1       TCTCGGCGTCTCTTCCGCCGTTTCCGCTACCAGGAGGCGGCGGGCCCCCGGGAGGCCCTG
                  ************************************************************

HsCD00329591      CAGCGCCTCTGGGACCTGTGCGGGGGCTGGCTGCGGCCTGAGAGGCACACCAAGGAGCAG
NM_032752.1       CAGCGCCTCTGGGACCTGTGCGGGGGCTGGCTGCGGCCTGAGAGGCACACCAAGGAGCAG
                  ************************************************************

HsCD00329591      ATTCTGGAGCTGCTGGTGCTGGAGCAGTTCCTGGCCATCCTGCCCCGGGAGATCCAGAGC
NM_032752.1       ATTCTGGAGCTGCTGGTGCTGGAGCAGTTCCTGGCCATCCTGCCCCGGGAGATCCAGAGC
                  ************************************************************

HsCD00329591      TGGGTGCGGGCGCAGGAGCCTGAGAGCGGAGAGCAGGCTGTGGCCGCGGTGGAGGCACTG
NM_032752.1       TGGGTGCGGGCGCAGGAGCCTGAGAGCGGAGAGCAGGCTGTGGCCGCGGTGGAGGCACTG
                  ************************************************************

HsCD00329591      GAACGGGAGCCCGGGAGACCCTGGCAGTGGCTCAAGCACTGTGAAGACCCTGTGGTGATT
NM_032752.1       GAACGGGAGCCCGGGAGACCCTGGCAGTGGCTCAAGCACTGTGAAGACCCTGTGGTGATT
                  ************************************************************

HsCD00329591      GATGATGGGGACAGCCCTCTGGACCAGGAGCAGGAGCAGCTGCCGGTAGAGCCCCACAGC
NM_032752.1       GATGATGGGGACAGCCCTCTGGACCAGGAGCAGGAGCAGCTGCCGGTAGAGCCCCACAGC
                  ************************************************************

HsCD00329591      GACCTTGCAAAGAACCAGGATGCCCAGCCCATAACCCTGGCACAGTGCCTGGGGCTCCCA
NM_032752.1       GACCTTGCAAAGAACCAGGATGCCCAGCCCATAACCCTGGCACAGTGCCTGGGGCTCCCA
                  ************************************************************

HsCD00329591      AGCAGACCACCAAGCCAGCTCAGCGGGGACCCAGTTCTGCAAGATGCTTTCCTTCTTCAG
NM_032752.1       AGCAGACCACCAAGCCAGCTCAGCGGGGACCCAGTTCTGCAAGATGCTTTCCTTCTTCAG
                  ************************************************************

HsCD00329591      GAAGAGAATGTGCGGGACACACAGCAGGTGACCACCCTCCAGCTACCCCCGAGTCGGGTT
NM_032752.1       GAAGAGAATGTGCGGGACACACAGCAGGTGACCACCCTCCAGCTACCCCCGAGTCGGGTT
                  ************************************************************

HsCD00329591      TCTCCATTCAAGGACATGATTTTATGCTTCTCTGAAGAGGATTGGTCCCTTCTAGATCCT
NM_032752.1       TCTCCATTCAAGGACATGATTTTATGCTTCTCTGAAGAGGATTGGTCCCTTCTAGATCCT
                  ************************************************************

HsCD00329591      GCCCAGACTGGCTTCTATGGAGAGTTCATCATTGGGGAGGATTACGGGGTCTCAATGCCT
NM_032752.1       GCCCAGACTGGCTTCTATGGAGAGTTCATCATTGGGGAGGATTACGGGGTCTCAATGCCT
                  ************************************************************

HsCD00329591      CCAAACGACCTAGCTGCCCAGCCAGATCTCTCCCAGGGAGAGGAGAATGAGCCACGCGTT
NM_032752.1       CCAAACGACCTAGCTGCCCAGCCAGATCTCTCCCAGGGAGAGGAGAATGAGCCACGCGTT
                  ************************************************************

HsCD00329591      CCAGAGTTGCAGGATCTCCAGGGCAAAGAAGTGCCCCAGGTCTCCTACTTGGACTCTCCA
NM_032752.1       CCAGAGTTGCAGGATCTCCAGGGCAAAGAAGTGCCCCAGGTCTCCTACTTGGACTCTCCA
                  ************************************************************

HsCD00329591      AGTCTCCAGCCATTCCAGGTAGAAGAAAGAAGGAAACGTGAGGAGCTGCAGGTGCCAGAG
NM_032752.1       AGTCTCCAGCCATTCCAGGTAGAAGAAAGAAGGAAACGTGAGGAGCTGCAGGTGCCAGAG
                  ************************************************************

HsCD00329591      TTCCAGGCCTGCCCACAGACGGTGGTGCCTCAAAACACCTACCCAGCTGGCGGCAACCCG
NM_032752.1       TTCCAGGCCTGCCCACAGACGGTGGTGCCTCAAAACACCTACCCAGCTGGCGGCAACCCG
                  ************************************************************

HsCD00329591      CGATCTCTAGAGAACAGCCTGGATGAAGAAGTGACCATCGAGATCGTTCTCTCCAGCTCT
NM_032752.1       CGATCTCTAGAGAACAGCCTGGATGAAGAAGTGACCATCGAGATCGTTCTCTCCAGCTCT
                  ************************************************************

HsCD00329591      GGGGACGAGGACTCCCAGCATGGCCCGTACTGCACAGAAGAGCTGGGGAGCCCCACAGAG
NM_032752.1       GGGGACGAGGACTCCCAGCATGGCCCGTACTGCACAGAAGAGCTGGGGAGCCCCACAGAG
                  ************************************************************

HsCD00329591      AAGCAGCGCAGCCTCCCCGCCTCCCACCGGAGCAGCACCGAGGCCGGAGGCGAGGTGCAG
NM_032752.1       AAGCAGCGCAGCCTCCCCGCCTCCCACCGGAGCAGCACCGAGGCCGGAGGCGAGGTGCAG
                  ************************************************************

HsCD00329591      ACCTCCAAGAAGTCCTACGTGTGTCCGAACTGTGGGAAAATCTTCCGCTGGAGGGTCAAC
NM_032752.1       ACCTCCAAGAAGTCCTACGTGTGTCCGAACTGTGGGAAAATCTTCCGCTGGAGGGTCAAC
                  ************************************************************

HsCD00329591      TTCATCCGGCATCTGCGGAGCCGCAGGGAGCAGGAGAAGCCGCACGAGTGCTCGGTGTGC
NM_032752.1       TTCATCCGGCATCTGCGGAGCCGCAGGGAGCAGGAGAAGCCGCACGAGTGCTCGGTGTGC
                  ************************************************************

HsCD00329591      GGGGAGCTGTTCAGCGACAGCGAGGACCTGGATGGGCACCTAGAGAGCCACGAGGCCCAG
NM_032752.1       GGGGAGCTGTTCAGCGACAGCGAGGACCTGGATGGGCACCTAGAGAGCCACGAGGCCCAG
                  ************************************************************

HsCD00329591      AAGCCTTACCGGTGTGGTGCCTGCGGGAAGAGCTTCCGCCTGAACTCCCACCTGCTCTCC
NM_032752.1       AAGCCTTACCGGTGTGGTGCCTGCGGGAAGAGCTTCCGCCTGAACTCCCACCTGCTCTCC
                  ************************************************************

HsCD00329591      CACCGGCGGATACACCTGCAGCCGGACAGACTCCAGCCGGTGGAGAAGAGAGAGCAGGCG
NM_032752.1       CACCGGCGGATACACCTGCAGCCGGACAGACTCCAGCCGGTGGAGAAGAGAGAGCAGGCG
                  ************************************************************

HsCD00329591      GCATCCGAGGACGCGGACAAGGGTCCCAAAGAGCCGCTGGAAAACGGCAAGGCCAAACTG
NM_032752.1       GCATCCGAGGACGCGGACAAGGGTCCCAAAGAGCCGCTGGAAAACGGCAAGGCCAAACTG
                  ************************************************************

HsCD00329591      AGCTTCCAGTGCTGTGAGTGTGGGAAGGCCTTCCAGCGGCACGACCACCTGGCTCGGCAC
NM_032752.1       AGCTTCCAGTGCTGTGAGTGTGGGAAGGCCTTCCAGCGGCACGACCACCTGGCTCGGCAC
                  ************************************************************

HsCD00329591      CGCAGCCACTTTCACCTGAAGGACAAAGCCCGGCCCTTCCAGTGCCGGTACTGCGTCAAG
NM_032752.1       CGCAGCCACTTTCACCTGAAGGACAAAGCCCGGCCCTTCCAGTGCCGGTACTGCGTCAAG
                  ************************************************************

HsCD00329591      AGCTTCACGCAGAACTATGACCTCCTCCGCCACGAGCGCCTGCACATGAAGCGCCGTTCC
NM_032752.1       AGCTTCACGCAGAACTATGACCTCCTCCGCCACGAGCGCCTGCACATGAAGCGCCGTTCC
                  ************************************************************

HsCD00329591      AAGCAGGCTCTGAACTCCTACTTG
NM_032752.1       AAGCAGGCTCTGAACTCCTACTGA
                  ********************** .

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
SMART domain : KRAB : krueppel associated box
SMART domain : SCAN : leucine rich region
UniProt : Q96IT1
HPRD : 15805

Clone

Cloning Information : 269093
HIP Master Clone ID : 169526
Original Clone ID : FLH269093.01L

Organism


TAX_ID : 9606
Species Specific ID: 84838

Genome

Chromosome : 1
Map Location : 1q44
Ensembl : ENSG00000162714

Reagents

Labome : ZNF496-antibody