DNASU Plasmid Repository • 480.965.5697 | Email

OTX1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  OTX1
Gene Name:  orthodenticle homeobox 1
Sequence             
Original Clone ID: FLH269095.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329593

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1065nts         Open reading frame : 1 to 1065
Reference Sequence Alignment

ATGATGTCTTACCTCAAACAACCCCCATACGGCATGAACGGGCTGGGCCTGGCCGGGCCC
GCCATGGACCTCCTGCACCCATCCGTGGGCTATCCGGCCACTCCGCGGAAGCAGCGGCGG
GAGCGCACCACCTTCACGCGTTCACAGCTGGACGTGCTCGAGGCGCTCTTCGCCAAGACT
CGCTACCCTGACATCTTCATGCGGGAGGAGGTGGCGCTCAAGATCAACCTGCCGGAGTCT
AGAGTCCAGGTCTGGTTCAAGAACCGCCGCGCCAAATGCCGCCAGCAGCAGCAGAGCGGG
AGCGGAACCAAGAGCCGCCCAGCCAAGAAGAAGTCCTCTCCAGTGCGGGAGAGCTCGGGC
TCCGAAAGCAGTGGCCAATTCACGCCGCCAGCTGTGTCCAGCTCTGCCTCGTCCTCTAGC
TCGGCGTCCAGCTCTTCCGCCAACCCAGCGGCTGCAGCGGCTGCGGGACTAGGTGGGAAC
CCGGTGGCGGCCGCGTCGTCGCTGAGTACACCAGCTGCCTCATCTATCTGGAGCCCGGCC
TCCATCTCGCCAGGCTCAGCGCCCGCGTCCGTGTCGGTGCCGGAGCCATTGGCCGCGCCT
AGCAACACCTCGTGTATGCAGCGCTCCGTAGCTGCAGGCGCCGCCACCGCAGCAGCCTCT
TATCCCATGTCCTACGGCCAGGGCGGCAGCTACGGCCAAGGCTACCCTACGCCCTCCTCT
TCCTACTTTGGCGGCGTGGACTGCAGCTCATACCTAGCGCCCATGCACTCACATCACCAC
CCGCACCAGCTGAGCCCCATGGCACCCTCCTCCATGGCGGGCCACCATCATCACCACCCA
CATGCGCACCACCCGTTGAGCCAGTCCTCAGGCCACCACCACCACCATCACCACCACCAC
CACCAAGGCTACGGTGGCTCTGGGCTTGCCTTCAACTCTGCCGACTGCTTGGATTACAAG
GAGCCTGGCGCCGCTGCTGCTTCCTCCGCCTGGAAACTCAACTTCAACTCCCCCGACTGT
CTGGACTATAAGGACCAAGCCTCATGGCGGTTCCAGGTCTTGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1285
Start on reference sequence 221
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : OTX1
Symbol Nomenclature : OTX1
Designation : homeobox protein OTX1|orthodenticle homolog 1
Full Nomenclature : orthodenticle homeobox 1
GENEID : 5013
HGNC : 8521
MIM : 600036
Vega : OTTHUMG00000129454
Target GenBank: BC007621

 

Reference Sequence Alignment

  

HsCD00329593        ATGATGTCTTACCTCAAACAACCCCCATACGGCATGAACGGGCTGGGCCTGGCCGGGCCC
NM_014562.3         ATGATGTCTTACCTCAAACAACCCCCATACGGCATGAACGGGCTGGGCCTGGCCGGGCCC
NM_001199770.1      ATGATGTCTTACCTCAAACAACCCCCATACGGCATGAACGGGCTGGGCCTGGCCGGGCCC
                    ************************************************************

HsCD00329593        GCCATGGACCTCCTGCACCCATCCGTGGGCTATCCGGCCACTCCGCGGAAGCAGCGGCGG
NM_014562.3         GCCATGGACCTCCTGCACCCATCCGTGGGCTATCCGGCCACTCCGCGGAAGCAGCGGCGG
NM_001199770.1      GCCATGGACCTCCTGCACCCATCCGTGGGCTATCCGGCCACTCCGCGGAAGCAGCGGCGG
                    ************************************************************

HsCD00329593        GAGCGCACCACCTTCACGCGTTCACAGCTGGACGTGCTCGAGGCGCTCTTCGCCAAGACT
NM_014562.3         GAGCGCACCACCTTCACGCGTTCACAGCTGGACGTGCTCGAGGCGCTCTTCGCCAAGACT
NM_001199770.1      GAGCGCACCACCTTCACGCGTTCACAGCTGGACGTGCTCGAGGCGCTCTTCGCCAAGACT
                    ************************************************************

HsCD00329593        CGCTACCCTGACATCTTCATGCGGGAGGAGGTGGCGCTCAAGATCAACCTGCCGGAGTCT
NM_014562.3         CGCTACCCTGACATCTTCATGCGGGAGGAGGTGGCGCTCAAGATCAACCTGCCGGAGTCT
NM_001199770.1      CGCTACCCTGACATCTTCATGCGGGAGGAGGTGGCGCTCAAGATCAACCTGCCGGAGTCT
                    ************************************************************

HsCD00329593        AGAGTCCAGGTCTGGTTCAAGAACCGCCGCGCCAAATGCCGCCAGCAGCAGCAGAGCGGG
NM_014562.3         AGAGTCCAGGTCTGGTTCAAGAACCGCCGCGCCAAATGCCGCCAGCAGCAGCAGAGCGGG
NM_001199770.1      AGAGTCCAGGTCTGGTTCAAGAACCGCCGCGCCAAATGCCGCCAGCAGCAGCAGAGCGGG
                    ************************************************************

HsCD00329593        AGCGGAACCAAGAGCCGCCCAGCCAAGAAGAAGTCCTCTCCAGTGCGGGAGAGCTCGGGC
NM_014562.3         AGCGGAACCAAGAGCCGCCCAGCCAAGAAGAAGTCCTCTCCAGTGCGGGAGAGCTCGGGC
NM_001199770.1      AGCGGAACCAAGAGCCGCCCAGCCAAGAAGAAGTCCTCTCCAGTGCGGGAGAGCTCGGGC
                    ************************************************************

HsCD00329593        TCCGAAAGCAGTGGCCAATTCACGCCGCCAGCTGTGTCCAGCTCTGCCTCGTCCTCTAGC
NM_014562.3         TCCGAAAGCAGTGGCCAATTCACGCCGCCAGCTGTGTCCAGCTCTGCCTCGTCCTCTAGC
NM_001199770.1      TCCGAAAGCAGTGGCCAATTCACGCCGCCAGCTGTGTCCAGCTCTGCCTCGTCCTCTAGC
                    ************************************************************

HsCD00329593        TCGGCGTCCAGCTCTTCCGCCAACCCAGCGGCTGCAGCGGCTGCGGGACTAGGTGGGAAC
NM_014562.3         TCGGCGTCCAGCTCTTCCGCCAACCCAGCGGCTGCAGCGGCTGCGGGACTAGGTGGGAAC
NM_001199770.1      TCGGCGTCCAGCTCTTCCGCCAACCCAGCGGCTGCAGCGGCTGCGGGACTAGGTGGGAAC
                    ************************************************************

HsCD00329593        CCGGTGGCGGCCGCGTCGTCGCTGAGTACACCAGCTGCCTCATCTATCTGGAGCCCGGCC
NM_014562.3         CCGGTGGCGGCCGCGTCGTCGCTGAGTACACCAGCTGCCTCATCTATCTGGAGCCCGGCC
NM_001199770.1      CCGGTGGCGGCCGCGTCGTCGCTGAGTACACCAGCTGCCTCATCTATCTGGAGCCCGGCC
                    ************************************************************

HsCD00329593        TCCATCTCGCCAGGCTCAGCGCCCGCGTCCGTGTCGGTGCCGGAGCCATTGGCCGCGCCT
NM_014562.3         TCCATCTCGCCAGGCTCAGCGCCCGCGTCCGTGTCGGTGCCGGAGCCATTGGCCGCGCCT
NM_001199770.1      TCCATCTCGCCAGGCTCAGCGCCCGCGTCCGTGTCGGTGCCGGAGCCATTGGCCGCGCCT
                    ************************************************************

HsCD00329593        AGCAACACCTCGTGTATGCAGCGCTCCGTAGCTGCAGGCGCCGCCACCGCAGCAGCCTCT
NM_014562.3         AGCAACACCTCGTGTATGCAGCGCTCCGTAGCTGCAGGCGCCGCCACCGCAGCAGCCTCT
NM_001199770.1      AGCAACACCTCGTGTATGCAGCGCTCCGTAGCTGCAGGCGCCGCCACCGCAGCAGCCTCT
                    ************************************************************

HsCD00329593        TATCCCATGTCCTACGGCCAGGGCGGCAGCTACGGCCAAGGCTACCCTACGCCCTCCTCT
NM_014562.3         TATCCCATGTCCTACGGCCAGGGCGGCAGCTACGGCCAAGGCTACCCTACGCCCTCCTCT
NM_001199770.1      TATCCCATGTCCTACGGCCAGGGCGGCAGCTACGGCCAAGGCTACCCTACGCCCTCCTCT
                    ************************************************************

HsCD00329593        TCCTACTTTGGCGGCGTGGACTGCAGCTCATACCTAGCGCCCATGCACTCACATCACCAC
NM_014562.3         TCCTACTTTGGCGGCGTGGACTGCAGCTCATACCTAGCGCCCATGCACTCACATCACCAC
NM_001199770.1      TCCTACTTTGGCGGCGTGGACTGCAGCTCATACCTAGCGCCCATGCACTCACATCACCAC
                    ************************************************************

HsCD00329593        CCGCACCAGCTGAGCCCCATGGCACCCTCCTCCATGGCGGGCCACCATCATCACCACCCA
NM_014562.3         CCGCACCAGCTCAGCCCCATGGCACCCTCCTCCATGGCGGGCCACCATCATCACCACCCA
NM_001199770.1      CCGCACCAGCTCAGCCCCATGGCACCCTCCTCCATGGCGGGCCACCATCATCACCACCCA
                    *********** ************************************************

HsCD00329593        CATGCGCACCACCCGTTGAGCCAGTCCTCAGGCCACCACCACCACCATCACCACCACCAC
NM_014562.3         CATGCGCACCACCCGTTGAGCCAGTCCTCAGGCCACCACCACCACCATCACCACCACCAC
NM_001199770.1      CATGCGCACCACCCGTTGAGCCAGTCCTCAGGCCACCACCACCACCATCACCACCACCAC
                    ************************************************************

HsCD00329593        CACCAAGGCTACGGTGGCTCTGGGCTTGCCTTCAACTCTGCCGACTGCTTGGATTACAAG
NM_014562.3         CACCAAGGCTACGGTGGCTCTGGGCTTGCCTTCAACTCTGCCGACTGCTTGGATTACAAG
NM_001199770.1      CACCAAGGCTACGGTGGCTCTGGGCTTGCCTTCAACTCTGCCGACTGCTTGGATTACAAG
                    ************************************************************

HsCD00329593        GAGCCTGGCGCCGCTGCTGCTTCCTCCGCCTGGAAACTCAACTTCAACTCCCCCGACTGT
NM_014562.3         GAGCCTGGCGCCGCTGCTGCTTCCTCCGCCTGGAAACTCAACTTCAACTCCCCCGACTGT
NM_001199770.1      GAGCCTGGCGCCGCTGCTGCTTCCTCCGCCTGGAAACTCAACTTCAACTCCCCCGACTGT
                    ************************************************************

HsCD00329593        CTGGACTATAAGGACCAAGCCTCATGGCGGTTCCAGGTCTTGTTG
NM_014562.3         CTGGACTATAAGGACCAAGCCTCATGGCGGTTCCAGGTCTTGTGA
NM_001199770.1      CTGGACTATAAGGACCAAGCCTCATGGCGGTTCCAGGTCTTGTGA
                    ******************************************* .

Protein

SMART domain : HOX : Homeodomain
UniProt : B3KTJ4
UniProt : P32242
HPRD : 08964

Clone

Cloning Information : 269095
HIP Master Clone ID : 169718
Original Clone ID : FLH269095.01L

Organism


TAX_ID : 9606
Species Specific ID: 5013

Genome

Chromosome : 2
Map Location : 2p13
Ensembl : ENSG00000115507

Reagents

Labome : OTX1-antibody