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GTF2H4 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  GTF2H4
Gene Name:  general transcription factor IIH, polypeptide 4, 52kDa
Sequence             
Original Clone ID: FLH269096.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329594

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1389nts         Open reading frame : 1 to 1389
Reference Sequence Alignment

ATGGAGAGCACCCCTTCAAGGGGACTGAACCGAGTACACCTACAATGCAGGAATCTGCAG
GAATTCTTAGGGGGCCTGAGCCCTGGGGTATTGGACCGATTGTATGGGCACCCTGCCACA
TGTCTGGCTGTCTTCAGGGAGCTCCCATCCTTGGCTAAGAACTGGGTGATGCGGATGCTC
TTTCTGGAGCAGCCTTTGCCACAGGCTGCTGTAGCTCTGTGGGTAAAGAAGGAATTCAGC
AAGGCTCAGGAGGAAAGTACAGGGCTGCTGAGCGGCCTCCGGATCTGGCACACACAGCTG
CTCCCAGGCGGGCTCCAGGGCCTCATCCTCAACCCCATTTTCCGCCAGAACCTCCGCATT
GCCCTTCTGGGTGGGGGGAAGGCCTGGTCTGATGACACAAGTCAGCTGGGACCAGACAAG
CATGCCCGGGACGTTCCCTCCCTTGACAAGTACGCCGAGGAGCGATGGGAGGTGGTCTTG
CACTTCATGGTGGGCTCCCCCAGTGCAGCTGTCAGCCAGGACTTGGCTCAGCTCCTCAGC
CAGGCTGGGCTCATGAAGAGTACTGAACCTGGAGAGCCGCCCTGCATTACTTCCGCTGGC
TTCCAGTTCCTGTTGCTGGACACCCCGGCTCAGCTCTGGTACTTTATGTTGCAGTATTTG
CAGACAGCCCAGAGCCGGGGCATGGACCTGGTAGAGATTCTCTCCTTCCTCTTCCAGCTC
AGCTTCTCTACTCTGGGCAAGGATTACTCTGTGGAAGGTATGAGTGATTCTCTGTTGAAC
TTCCTGCAACATCTGCGTGAGTTTGGGCTTGTTTTCCAGAGGAAGAGGAAATCTCGGCGT
TACTACCCCACACGCCTGGCCATCAATCTCTCATCAGGTGTCTCTGGAGCTGGGGGCACT
GTGCATCAGCCAGGTTTCATTGTCGTGGAAACCAATTACCGACTGTATGCCTACACGGAG
TCGGAGCTGCAGATTGCCCTCATTGCCCTCTTCTCTGAGATGCTCTATCGGTTCCCCAAC
ATGGTGGTGGCGCAGGTGACCCGGGAGAGTGTGCAGCAGGCAATCGCCAGTGGCATCACA
GCCCAGCAGATAATCCATTTCCTAAGGACAAGAGCCCACCCAGTGATGCTCAAACAGACA
CCTGTGCTGCCCCCCACCATCACCGACCAGATCCGGCTCTGGGAGCTGGAAAGGGACAGA
CTCCGGTTCACTGAGGGTGTCCTGTATAACCAGTTCCTGTCGCAAGTGGACTTTGAGCTG
CTGCTGGCCCACGCGCGGGAGCTGGGCGTGCTCGTGTTCGAGAACTCGGCCAAGCGGCTC
ATGGTGGTGACCCCGGCCGGGCACAGCGACGTCAAGCGCTTTTGGAAGCGGCAGAAACAT
AGCTCCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1568
Start on reference sequence 180
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GTF2H4
Symbol Nomenclature : GTF2H4
SYNONYM : P52; TFB2; TFIIH
Designation : BTF2 p52|TFIIH basal transcription factor complex p52 subunit|basic transcription factor 2 52 kDa subunit|general transcription factor IIH polypeptide 4|general transcription factor IIH subunit 4
Full Nomenclature : general transcription factor IIH, polypeptide 4, 52kDa
GENEID : 2968
Locus Tag : DADB-160J14.4
HGNC : 4658
MIM : 601760
Vega : OTTHUMG00000031043
Target GenBank: BC004935

 

Reference Sequence Alignment

  

HsCD00329594      ATGGAGAGCACCCCTTCAAGGGGACTGAACCGAGTACACCTACAATGCAGGAATCTGCAG
NM_001517.4       ATGGAGAGCACCCCTTCAAGGGGACTGAACCGAGTACACCTACAATGCAGGAATCTGCAG
                  ************************************************************

HsCD00329594      GAATTCTTAGGGGGCCTGAGCCCTGGGGTATTGGACCGATTGTATGGGCACCCTGCCACA
NM_001517.4       GAATTCTTAGGGGGCCTGAGCCCTGGGGTATTGGACCGATTGTATGGGCACCCTGCCACA
                  ************************************************************

HsCD00329594      TGTCTGGCTGTCTTCAGGGAGCTCCCATCCTTGGCTAAGAACTGGGTGATGCGGATGCTC
NM_001517.4       TGTCTGGCTGTCTTCAGGGAGCTCCCATCCTTGGCTAAGAACTGGGTGATGCGGATGCTC
                  ************************************************************

HsCD00329594      TTTCTGGAGCAGCCTTTGCCACAGGCTGCTGTAGCTCTGTGGGTAAAGAAGGAATTCAGC
NM_001517.4       TTTCTGGAGCAGCCTTTGCCACAGGCTGCTGTAGCTCTGTGGGTAAAGAAGGAATTCAGC
                  ************************************************************

HsCD00329594      AAGGCTCAGGAGGAAAGTACAGGGCTGCTGAGCGGCCTCCGGATCTGGCACACACAGCTG
NM_001517.4       AAGGCTCAGGAGGAAAGTACAGGGCTGCTGAGCGGCCTCCGGATCTGGCACACACAGCTG
                  ************************************************************

HsCD00329594      CTCCCAGGCGGGCTCCAGGGCCTCATCCTCAACCCCATTTTCCGCCAGAACCTCCGCATT
NM_001517.4       CTCCCAGGCGGGCTCCAGGGCCTCATCCTCAACCCCATTTTCCGCCAGAACCTCCGCATT
                  ************************************************************

HsCD00329594      GCCCTTCTGGGTGGGGGGAAGGCCTGGTCTGATGACACAAGTCAGCTGGGACCAGACAAG
NM_001517.4       GCCCTTCTGGGTGGGGGGAAGGCCTGGTCTGATGACACAAGTCAGCTGGGACCAGACAAG
                  ************************************************************

HsCD00329594      CATGCCCGGGACGTTCCCTCCCTTGACAAGTACGCCGAGGAGCGATGGGAGGTGGTCTTG
NM_001517.4       CATGCCCGGGACGTTCCCTCCCTTGACAAGTACGCCGAGGAGCGATGGGAGGTGGTCTTG
                  ************************************************************

HsCD00329594      CACTTCATGGTGGGCTCCCCCAGTGCAGCTGTCAGCCAGGACTTGGCTCAGCTCCTCAGC
NM_001517.4       CACTTCATGGTGGGCTCCCCCAGTGCAGCTGTCAGCCAGGACTTGGCTCAGCTCCTCAGC
                  ************************************************************

HsCD00329594      CAGGCTGGGCTCATGAAGAGTACTGAACCTGGAGAGCCGCCCTGCATTACTTCCGCTGGC
NM_001517.4       CAGGCTGGGCTCATGAAGAGTACTGAACCTGGAGAGCCGCCCTGCATTACTTCCGCTGGC
                  ************************************************************

HsCD00329594      TTCCAGTTCCTGTTGCTGGACACCCCGGCTCAGCTCTGGTACTTTATGTTGCAGTATTTG
NM_001517.4       TTCCAGTTCCTGTTGCTGGACACCCCGGCTCAGCTCTGGTACTTTATGTTGCAGTATTTG
                  ************************************************************

HsCD00329594      CAGACAGCCCAGAGCCGGGGCATGGACCTGGTAGAGATTCTCTCCTTCCTCTTCCAGCTC
NM_001517.4       CAGACAGCCCAGAGCCGGGGCATGGACCTGGTAGAGATTCTCTCCTTCCTCTTCCAGCTC
                  ************************************************************

HsCD00329594      AGCTTCTCTACTCTGGGCAAGGATTACTCTGTGGAAGGTATGAGTGATTCTCTGTTGAAC
NM_001517.4       AGCTTCTCTACTCTGGGCAAGGATTACTCTGTGGAAGGTATGAGTGATTCTCTGTTGAAC
                  ************************************************************

HsCD00329594      TTCCTGCAACATCTGCGTGAGTTTGGGCTTGTTTTCCAGAGGAAGAGGAAATCTCGGCGT
NM_001517.4       TTCCTGCAACATCTGCGTGAGTTTGGGCTTGTTTTCCAGAGGAAGAGGAAATCTCGGCGT
                  ************************************************************

HsCD00329594      TACTACCCCACACGCCTGGCCATCAATCTCTCATCAGGTGTCTCTGGAGCTGGGGGCACT
NM_001517.4       TACTACCCCACACGCCTGGCCATCAATCTCTCATCAGGTGTCTCTGGAGCTGGGGGCACT
                  ************************************************************

HsCD00329594      GTGCATCAGCCAGGTTTCATTGTCGTGGAAACCAATTACCGACTGTATGCCTACACGGAG
NM_001517.4       GTGCATCAGCCAGGTTTCATTGTCGTGGAAACCAATTACCGACTGTATGCCTACACGGAG
                  ************************************************************

HsCD00329594      TCGGAGCTGCAGATTGCCCTCATTGCCCTCTTCTCTGAGATGCTCTATCGGTTCCCCAAC
NM_001517.4       TCGGAGCTGCAGATTGCCCTCATTGCCCTCTTCTCTGAGATGCTCTATCGGTTCCCCAAC
                  ************************************************************

HsCD00329594      ATGGTGGTGGCGCAGGTGACCCGGGAGAGTGTGCAGCAGGCAATCGCCAGTGGCATCACA
NM_001517.4       ATGGTGGTGGCGCAGGTGACCCGGGAGAGTGTGCAGCAGGCAATCGCCAGTGGCATCACA
                  ************************************************************

HsCD00329594      GCCCAGCAGATAATCCATTTCCTAAGGACAAGAGCCCACCCAGTGATGCTCAAACAGACA
NM_001517.4       GCCCAGCAGATAATCCATTTCCTAAGGACAAGAGCCCACCCAGTGATGCTCAAACAGACA
                  ************************************************************

HsCD00329594      CCTGTGCTGCCCCCCACCATCACCGACCAGATCCGGCTCTGGGAGCTGGAAAGGGACAGA
NM_001517.4       CCTGTGCTGCCCCCCACCATCACCGACCAGATCCGGCTCTGGGAGCTGGAAAGGGACAGA
                  ************************************************************

HsCD00329594      CTCCGGTTCACTGAGGGTGTCCTGTATAACCAGTTCCTGTCGCAAGTGGACTTTGAGCTG
NM_001517.4       CTCCGGTTCACTGAGGGTGTCCTGTATAACCAGTTCCTGTCGCAAGTGGACTTTGAGCTG
                  ************************************************************

HsCD00329594      CTGCTGGCCCACGCGCGGGAGCTGGGCGTGCTCGTGTTCGAGAACTCGGCCAAGCGGCTC
NM_001517.4       CTGCTGGCCCACGCGCGGGAGCTGGGCGTGCTCGTGTTCGAGAACTCGGCCAAGCGGCTC
                  ************************************************************

HsCD00329594      ATGGTGGTGACCCCGGCCGGGCACAGCGACGTCAAGCGCTTTTGGAAGCGGCAGAAACAT
NM_001517.4       ATGGTGGTGACCCCGGCCGGGCACAGCGACGTCAAGCGCTTTTGGAAGCGGCAGAAACAT
                  ************************************************************

HsCD00329594      AGCTCCTTG
NM_001517.4       AGCTCCTGA
                  ******* .

Pathways

Panther : General transcription regulation
Panther : Transcription regulation by bZIP transcription factor
Reactome : DNA Repair
Reactome : Disease
Reactome : Dual incision reaction in GG-NER
Reactome : Dual incision reaction in TC-NER
Reactome : Epigenetic regulation of gene expression
Reactome : Formation of HIV elongation complex in the absence of HIV Tat
Reactome : Formation of HIV-1 elongation complex containing HIV-1 Tat
Reactome : Formation of RNA Pol II elongation complex
Reactome : Formation of incision complex in GG-NER
Reactome : Formation of the Early Elongation Complex
Reactome : Formation of the HIV-1 Early Elongation Complex
Reactome : Formation of transcription-coupled NER (TC-NER) repair complex
Reactome : Gene Expression
Reactome : Global Genomic NER (GG-NER)
Reactome : HIV Infection
Reactome : HIV Life Cycle
Reactome : HIV Transcription Elongation
Reactome : HIV Transcription Initiation
Reactome : Late Phase of HIV Life Cycle
Reactome : Negative epigenetic regulation of rRNA expression
Reactome : NoRC negatively regulates rRNA expression
Reactome : Nucleotide Excision Repair
Reactome : RNA Pol II CTD phosphorylation and interaction with CE
Reactome : RNA Polymerase I Chain Elongation
Reactome : RNA Polymerase I Promoter Clearance
Reactome : RNA Polymerase I Promoter Escape
Reactome : RNA Polymerase I Transcription
Reactome : RNA Polymerase I Transcription Initiation
Reactome : RNA Polymerase I Transcription Termination
Reactome : RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
Reactome : RNA Polymerase II HIV Promoter Escape
Reactome : RNA Polymerase II Pre-transcription Events
Reactome : RNA Polymerase II Promoter Escape
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Elongation
Reactome : RNA Polymerase II Transcription Initiation
Reactome : RNA Polymerase II Transcription Initiation And Promoter Clearance
Reactome : RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
Reactome : Tat-mediated elongation of the HIV-1 transcript
Reactome : Transcription
Reactome : Transcription of the HIV genome
Reactome : Transcription-coupled NER (TC-NER)
Reactome : mRNA Capping
WikiPathway : Eukaryotic Transcription Initiation

Protein

UniProt : Q76KU4
UniProt : Q92759
HPRD : 03456

Clone

Cloning Information : 269096
HIP Master Clone ID : 169258
Original Clone ID : FLH269096.01L

Organism


TAX_ID : 9606
Species Specific ID: 2968

Genome

Chromosome : 6
Map Location : 6p21.3
Ensembl : ENSG00000213780

Reagents

Labome : GTF2H4-antibody