DNASU Plasmid Repository • 480.965.5697 | Email

HLX1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  HLX
Gene Name:  H2.0-like homeobox
Sequence             
Original Clone ID: FLH269102.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329600

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1467nts         Open reading frame : 1 to 1467
Reference Sequence Alignment

ATGTTCGCAGCCGGGCTGGCTCCCTTCTACGCCTCCAACTTCAGCCTCTGGTCGGCCGCT
TACTGCTCCTCGGCCGGCCCAGGCGGCTGCTCCTTCCCCTTGGACCCCGCCGCCGTCAAA
AAGCCCTCCTTCTGCATCGCAGACATTCTGCACGCCGGCGTGGGGGATCTGGGGGCGGCC
CCGGAGGGCCTGGCAGGGGCCTCGGCCGCCGCCCTCACCGCGCACTTGGGCTCGGTTCAC
CCGCACGCCTCTTTCCAAGCGGCGGCCAGATCCCCGCTTCGACCCACCCCAGTGGTGGCG
CCCTCCGAAGTCCCGGCTGGCTTCCCGCAGCGGCTGTCTCCGCTCTCAGCCGCCTACCAC
CACCATCACCCGCAACAACAACAGCAGCAGCAACAGCCGCAGCAGCAACAGCCTCCGCCT
CCGCCCCGGGCTGGCGCCCTGCAGCCCCCGGCCTCGGGGACGCGAGTGGTTCCGAACCCC
CACCACAGTGGCTCTGCCCCGGCCCCCTCCAGCAAAGACCTCAAATTTGGAATTGACCGC
ATTTTATCTGCAGAATTTGACCCAAAAGTCAAAGAAGGCAACACGCTGAGAGATCTCACT
TCCCTGCTAACCGGTGGGCGGCCCGCCGGGGTGCACCTCTCAGGCCTGCAGCCCTCGGCC
GGCCAGTTCTTCGCATCTCTAGATCCCATTAACGAGGCTTCTGCAATCCTGAGTCCCTTA
AACTCGAACCCAAGAAATTCAGTTCAGCATCAGTTCCAAGACACGTTTCCAGGTCCCTAT
GCTGTGCTCACGAAGGACACCATGCCGCAGACGTACAAAAGGAAGCGTTCATGGTCGCGC
GCTGTGTTCTCCAACCTGCAGAGGAAAGGCCTGGAGAAAAGGTTTGAGATTCAGAAGTAC
GTGACCAAGCCGGACCGAAAGCAGCTGGCGGCGATGCTGGGCCTCACGGACGCACAGGTG
AAGGTGTGGTTCCAGAACCGGCGGATGAAGTGGCGGCACTCCAAGGAGGCCCAGGCCCAA
AAGGACAAGGACAAGGAGGCTGGCGAGAAGCCATCAGGTGGAGCCCCGGCTGCGGATGGC
GAGCAGGACGAGAGGAGCCCCAGCCGTTCTGAAGGCGAGGCTGAGAGCGAGAGCAGCGAC
TCCGAGTCCCTGGACATGGCCCCCAGCGACACGGAGCGGACTGAGGGGAGTGAGCGTTCT
CTGCACCAAACAACAGTTATTAAGGCCCCGGTCACTGGCGCCCTCATTACCGCCAGCAGT
GCTGGGAGTGGTGGGAGCAGCGGCGGCGGCGGCAATAGTTTCAGCTTCAGCAGCGCCAGC
AGTCTTAGTAGCAGCAGCACCAGTGCGGGTTGCGCCAGCAGCCTTGGCGGCGGCGGCGCC
TCGGAGCTTCTCCCTGCAACACAGCCCACAGCCAGCAGCGCTCCCAAAAGCCCCGAGCCA
GCCCAAGGCGCGCTTGGCTGCTTATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1909
Start on reference sequence 443
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HLX
Symbol Nomenclature : HLX
SYNONYM : HB24; HLX1
Designation : H2.0-like homeo box 1|H2.0-like homeo box-1|H2.0-like homeobox 1|H2.0-like homeobox protein|homeobox protein HB24|homeobox protein HLX1
Full Nomenclature : H2.0-like homeobox
GENEID : 3142
Locus Tag : RP11-295M18.3
HGNC : 4978
MIM : 142995
Target GenBank: BC007294

 

Reference Sequence Alignment

  

HsCD00329600      ATGTTCGCAGCCGGGCTGGCTCCCTTCTACGCCTCCAACTTCAGCCTCTGGTCGGCCGCT
NM_021958.3       ATGTTCGCAGCCGGGCTGGCTCCCTTCTACGCCTCCAACTTCAGCCTCTGGTCGGCCGCT
                  ************************************************************

HsCD00329600      TACTGCTCCTCGGCCGGCCCAGGCGGCTGCTCCTTCCCCTTGGACCCCGCCGCCGTCAAA
NM_021958.3       TACTGCTCCTCGGCCGGCCCAGGCGGCTGCTCCTTCCCCTTGGACCCCGCCGCCGTCAAA
                  ************************************************************

HsCD00329600      AAGCCCTCCTTCTGCATCGCAGACATTCTGCACGCCGGCGTGGGGGATCTGGGGGCGGCC
NM_021958.3       AAGCCCTCCTTCTGCATCGCAGACATTCTGCACGCCGGCGTGGGGGATCTGGGGGCGGCC
                  ************************************************************

HsCD00329600      CCGGAGGGCCTGGCAGGGGCCTCGGCCGCCGCCCTCACCGCGCACTTGGGCTCGGTTCAC
NM_021958.3       CCGGAGGGCCTGGCAGGGGCCTCGGCCGCCGCCCTCACCGCGCACTTGGGCTCGGTTCAC
                  ************************************************************

HsCD00329600      CCGCACGCCTCTTTCCAAGCGGCGGCCAGATCCCCGCTTCGACCCACCCCAGTGGTGGCG
NM_021958.3       CCGCACGCCTCTTTCCAAGCGGCGGCCAGATCCCCGCTTCGACCCACCCCAGTGGTGGCG
                  ************************************************************

HsCD00329600      CCCTCCGAAGTCCCGGCTGGCTTCCCGCAGCGGCTGTCTCCGCTCTCAGCCGCCTACCAC
NM_021958.3       CCCTCCGAAGTCCCGGCTGGCTTCCCGCAGCGGCTGTCTCCGCTCTCAGCCGCCTACCAC
                  ************************************************************

HsCD00329600      CACCATCACCCGCAACAACAACAGCAGCAGCAACAGCCGCAGCAGCAACAGCCTCCGCCT
NM_021958.3       CACCATCACCCGCAACAACAACAGCAGCAGCAACAGCCGCAGCAGCAACAGCCTCCGCCT
                  ************************************************************

HsCD00329600      CCGCCCCGGGCTGGCGCCCTGCAGCCCCCGGCCTCGGGGACGCGAGTGGTTCCGAACCCC
NM_021958.3       CCGCCCCGGGCTGGCGCCCTGCAGCCCCCGGCCTCGGGGACGCGAGTGGTTCCGAACCCC
                  ************************************************************

HsCD00329600      CACCACAGTGGCTCTGCCCCGGCCCCCTCCAGCAAAGACCTCAAATTTGGAATTGACCGC
NM_021958.3       CACCACAGTGGCTCTGCCCCGGCCCCCTCCAGCAAAGACCTCAAATTTGGAATTGACCGC
                  ************************************************************

HsCD00329600      ATTTTATCTGCAGAATTTGACCCAAAAGTCAAAGAAGGCAACACGCTGAGAGATCTCACT
NM_021958.3       ATTTTATCTGCAGAATTTGACCCAAAAGTCAAAGAAGGCAACACGCTGAGAGATCTCACT
                  ************************************************************

HsCD00329600      TCCCTGCTAACCGGTGGGCGGCCCGCCGGGGTGCACCTCTCAGGCCTGCAGCCCTCGGCC
NM_021958.3       TCCCTGCTAACCGGTGGGCGGCCCGCCGGGGTGCACCTCTCAGGCCTGCAGCCCTCGGCC
                  ************************************************************

HsCD00329600      GGCCAGTTCTTCGCATCTCTAGATCCCATTAACGAGGCTTCTGCAATCCTGAGTCCCTTA
NM_021958.3       GGCCAGTTCTTCGCATCTCTAGATCCCATTAACGAGGCTTCTGCAATCCTGAGTCCCTTA
                  ************************************************************

HsCD00329600      AACTCGAACCCAAGAAATTCAGTTCAGCATCAGTTCCAAGACACGTTTCCAGGTCCCTAT
NM_021958.3       AACTCGAACCCAAGAAATTCAGTTCAGCATCAGTTCCAAGACACGTTTCCAGGTCCCTAT
                  ************************************************************

HsCD00329600      GCTGTGCTCACGAAGGACACCATGCCGCAGACGTACAAAAGGAAGCGTTCATGGTCGCGC
NM_021958.3       GCTGTGCTCACGAAGGACACCATGCCGCAGACGTACAAAAGGAAGCGTTCATGGTCGCGC
                  ************************************************************

HsCD00329600      GCTGTGTTCTCCAACCTGCAGAGGAAAGGCCTGGAGAAAAGGTTTGAGATTCAGAAGTAC
NM_021958.3       GCTGTGTTCTCCAACCTGCAGAGGAAAGGCCTGGAGAAAAGGTTTGAGATTCAGAAGTAC
                  ************************************************************

HsCD00329600      GTGACCAAGCCGGACCGAAAGCAGCTGGCGGCGATGCTGGGCCTCACGGACGCACAGGTG
NM_021958.3       GTGACCAAGCCGGACCGAAAGCAGCTGGCGGCGATGCTGGGCCTCACGGACGCACAGGTG
                  ************************************************************

HsCD00329600      AAGGTGTGGTTCCAGAACCGGCGGATGAAGTGGCGGCACTCCAAGGAGGCCCAGGCCCAA
NM_021958.3       AAGGTGTGGTTCCAGAACCGGCGGATGAAGTGGCGGCACTCCAAGGAGGCCCAGGCCCAA
                  ************************************************************

HsCD00329600      AAGGACAAGGACAAGGAGGCTGGCGAGAAGCCATCAGGTGGAGCCCCGGCTGCGGATGGC
NM_021958.3       AAGGACAAGGACAAGGAGGCTGGCGAGAAGCCATCAGGTGGAGCCCCGGCTGCGGATGGC
                  ************************************************************

HsCD00329600      GAGCAGGACGAGAGGAGCCCCAGCCGTTCTGAAGGCGAGGCTGAGAGCGAGAGCAGCGAC
NM_021958.3       GAGCAGGACGAGAGGAGCCCCAGCCGTTCTGAAGGCGAGGCTGAGAGCGAGAGCAGCGAC
                  ************************************************************

HsCD00329600      TCCGAGTCCCTGGACATGGCCCCCAGCGACACGGAGCGGACTGAGGGGAGTGAGCGTTCT
NM_021958.3       TCCGAGTCCCTGGACATGGCCCCCAGCGACACGGAGCGGACTGAGGGGAGTGAGCGTTCT
                  ************************************************************

HsCD00329600      CTGCACCAAACAACAGTTATTAAGGCCCCGGTCACTGGCGCCCTCATTACCGCCAGCAGT
NM_021958.3       CTGCACCAAACAACAGTTATTAAGGCCCCGGTCACTGGCGCCCTCATTACCGCCAGCAGT
                  ************************************************************

HsCD00329600      GCTGGGAGTGGTGGGAGCAGCGGCGGCGGCGGCAATAGTTTCAGCTTCAGCAGCGCCAGC
NM_021958.3       GCTGGGAGTGGTGGGAGCAGCGGCGGCGGCGGCAATAGTTTCAGCTTCAGCAGCGCCAGC
                  ************************************************************

HsCD00329600      AGTCTTAGTAGCAGCAGCACCAGTGCGGGTTGCGCCAGCAGCCTTGGCGGCGGCGGCGCC
NM_021958.3       AGTCTTAGTAGCAGCAGCACCAGTGCGGGTTGCGCCAGCAGCCTTGGCGGCGGCGGCGCC
                  ************************************************************

HsCD00329600      TCGGAGCTTCTCCCTGCAACACAGCCCACAGCCAGCAGCGCTCCCAAAAGCCCCGAGCCA
NM_021958.3       TCGGAGCTTCTCCCTGCAACACAGCCCACAGCCAGCAGCGCTCCCAAAAGCCCCGAGCCA
                  ************************************************************

HsCD00329600      GCCCAAGGCGCGCTTGGCTGCTTATTG
NM_021958.3       GCCCAAGGCGCGCTTGGCTGCTTATAG
                  *************************:*

Pathways

NCI : IL12-mediated signaling events

Protein

SMART domain : HOX : Homeodomain
UniProt : Q14774
HPRD : 00875

Clone

Cloning Information : 269102
HIP Master Clone ID : 169386
Original Clone ID : FLH269102.01L

Organism


TAX_ID : 9606
Species Specific ID: 3142

Genome

Chromosome : 1
Map Location : 1q41

Reagents

Labome : HLX-antibody