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GABPB2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  GABPB1
Gene Name:  GA binding protein transcription factor, beta subunit 1
Sequence             
Original Clone ID: FLH269118.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329616

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1152nts         Open reading frame : 1 to 1152
Reference Sequence Alignment

ATGTCCCTGGTAGATTTGGGAAAGAAGCTTTTAGAAGCGGCACGAGCAGGTCAAGATGAT
GAAGTTCGTATTTTGATGGCAAATGGAGCTCCCTTTACTACAGACTGGCTGGGAACTTCT
CCACTTCATCTAGCAGCACAGTATGGTCATTATTCCACCACAGAGGTACTGCTGCGAGCT
GGTGTGAGCAGAGATGCCAGAACCAAAGTGGACCGAACACCATTACATATGGCAGCTTCT
GAGGGCCATGCCAGCATAGTAGAGGTTTTACTTAAGCATGGTGCTGATGTCAATGCAAAG
GACATGTTAAAGATGACAGCTCTCCATTGGGCCACAGAACACAATCATCAAGAGGTGGTG
GAACTTTTAATCAAATATGGTGCTGATGTACACACGCAAAGTAAATTTTGTAAAACTGCA
TTTGATATTTCAATAGACAATGGAAATGAAGATTTAGCAGAGATATTACAGATTGCTATG
CAGAACCAAATCAACACAAACCCAGAGAGTCCTGACACTGTGACAATACATGCTGCAACA
CCACAGTTTATCATTGGACCTGGAGGGGTGGTGAACCTAACAGATGAAACGGGTGTATCT
GCTGTTCAGTTTGGAAACTCTTCTACATCAGTATTAGCTACATTAGCTGCCTTAGCTGAA
GCATCTGCTCCATTGTCCAATTCTTCAGAAACTCCAGTAGTGGCCACAGAAGAAGTAGTT
ACTGCAGAATCTGTGGATGGTGCCATTCAGCAAGTAGTTAGTTCAGGGGGTCAGCAAGTC
ATCGCAATAGTTACAGATGGAATTCAGCTTGGAAATTTGCACTCTATTCCAACCAGTGGA
ATTGGTCAGCCCATCATTGTGACCATGCCAGATGGACAACAAGTATTAACAGTACCAGCA
ACAGACATTGCTGAAGAAACTGTTATAAGTGAAGAACCACCAGCTAAGAGACAATGTATC
GAAATAATTGAAAACCGGGTGGAATCTGCAGAAATAGAAGAGAGAGAAGCTCTTCAGAAA
CAGCTGGATGAAGCAAATCGAGAAGCACAAAAATATCGACAGCAGCTCCTAAAGAAAGAA
CAGGAAGCAGAGGCCTACAGACAGAAGTTGGAAGCTATGACTCGTCTTCAGACTAATAAA
GAAGCTGTTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1370
Start on reference sequence 219
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GABPB1
Symbol Nomenclature : GABPB1
SYNONYM : BABPB2; E4TF1; E4TF1-47; E4TF1-53; E4TF1B; GABPB; GABPB2; NRF2B1; NRF2B2
Designation : GA binding protein transcription factor beta subunit 1 transcript variant gamma-2|GA-binding protein subunit beta-1|GABP subunit beta-2|GABPB-1|nuclear respiratory factor 2|transcription factor E4TF1-47|transcription factor E4TF1-53
Full Nomenclature : GA binding protein transcription factor, beta subunit 1
GENEID : 2553
HGNC : 4074
MIM : 600610
Vega : OTTHUMG00000131642
Target GenBank: BC036080

 

Reference Sequence Alignment

  

HsCD00329616      ATGTCCCTGGTAGATTTGGGAAAGAAGCTTTTAGAAGCGGCACGAGCAGGTCAAGATGAT
NM_005254.5       ATGTCCCTGGTAGATTTGGGAAAGAAGCTTTTAGAAGCGGCACGAGCAGGTCAAGATGAT
NM_016654.4       ATGTCCCTGGTAGATTTGGGAAAGAAGCTTTTAGAAGCGGCACGAGCAGGTCAAGATGAT
                  ************************************************************

HsCD00329616      GAAGTTCGTATTTTGATGGCAAATGGAGCTCCCTTTACTACAGACTGGCTGGGAACTTCT
NM_005254.5       GAAGTTCGTATTTTGATGGCAAATGGAGCTCCCTTTACTACAGACTGGCTGGGAACTTCT
NM_016654.4       GAAGTTCGTATTTTGATGGCAAATGGAGCTCCCTTTACTACAGACTGGCTGGGAACTTCT
                  ************************************************************

HsCD00329616      CCACTTCATCTAGCAGCACAGTATGGTCATTATTCCACCACAGAGGTACTGCTGCGAGCT
NM_005254.5       CCACTTCATCTAGCAGCACAGTATGGTCATTATTCCACCACAGAGGTACTGCTGCGAGCT
NM_016654.4       CCACTTCATCTAGCAGCACAGTATGGTCATTATTCCACCACAGAGGTACTGCTGCGAGCT
                  ************************************************************

HsCD00329616      GGTGTGAGCAGAGATGCCAGAACCAAAGTGGACCGAACACCATTACATATGGCAGCTTCT
NM_005254.5       GGTGTGAGCAGAGATGCCAGAACCAAAGTGGACCGAACACCATTACATATGGCAGCTTCT
NM_016654.4       GGTGTGAGCAGAGATGCCAGAACCAAAGTGGACCGAACACCATTACATATGGCAGCTTCT
                  ************************************************************

HsCD00329616      GAGGGCCATGCCAGCATAGTAGAGGTTTTACTTAAGCATGGTGCTGATGTCAATGCAAAG
NM_005254.5       GAGGGCCATGCCAGCATAGTAGAGGTTTTACTTAAGCATGGTGCTGATGTCAATGCAAAG
NM_016654.4       GAGGGCCATGCCAGCATAGTAGAGGTTTTACTTAAGCATGGTGCTGATGTCAATGCAAAG
                  ************************************************************

HsCD00329616      GACATGTTAAAGATGACAGCTCTCCATTGGGCCACAGAACACAATCATCAAGAGGTGGTG
NM_005254.5       GACATGTTAAAGATGACAGCTCTCCATTGGGCCACAGAACACAATCATCAAGAGGTGGTG
NM_016654.4       GACATGTTAAAGATGACAGCTCTCCATTGGGCCACAGAACACAATCATCAAGAGGTGGTG
                  ************************************************************

HsCD00329616      GAACTTTTAATCAAATATGGTGCTGATGTACACACGCAAAGTAAATTTTGTAAAACTGCA
NM_005254.5       GAACTTTTAATCAAATATGGTGCTGATGTACACACGCAAAGTAAATTTTGTAAAACTGCA
NM_016654.4       GAACTTTTAATCAAATATGGTGCTGATGTACACACGCAAAGTAAATTTTGTAAAACTGCA
                  ************************************************************

HsCD00329616      TTTGATATTTCAATAGACAATGGAAATGAAGATTTAGCAGAGATATTACAGATTGCTATG
NM_005254.5       TTTGATATTTCAATAGACAATGGAAATGAAGATTTAGCAGAGATATTACAGATTGCTATG
NM_016654.4       TTTGATATTTCAATAGACAATGGAAATGAAGATTTAGCAGAGATATTACAGATTGCTATG
                  ************************************************************

HsCD00329616      CAGAACCAAATCAACACAAACCCAGAGAGTCCTGACACTGTGACAATACATGCTGCAACA
NM_005254.5       CAGAACCAAATCAACACAAACCCAGAGAGTCCTGACACTGTGACAATACATGCTGCAACA
NM_016654.4       CAGAACCAAATCAACACAAACCCAGAGAGTCCTGACACTGTGACAATACATGCTGCAACA
                  ************************************************************

HsCD00329616      CCACAGTTTATCATTGGACCTGGAGGGGTGGTGAACCTAACAG-----------------
NM_005254.5       CCACAGTTTATCATTGGACCTGGAGGGGTGGTGAACCTAACAGGTCTGGTATCTTCAGAA
NM_016654.4       CCACAGTTTATCATTGGACCTGGAGGGGTGGTGAACCTAACAG-----------------
                  *******************************************                 

HsCD00329616      -------------------ATGAAACGGGTGTATCTGCTGTTCAGTTTGGAAACTCTTCT
NM_005254.5       AATTCATCCAAGGCAACAGATGAAACGGGTGTATCTGCTGTTCAGTTTGGAAACTCTTCT
NM_016654.4       -------------------ATGAAACGGGTGTATCTGCTGTTCAGTTTGGAAACTCTTCT
                                     *****************************************

HsCD00329616      ACATCAGTATTAGCTACATTAGCTGCCTTAGCTGAAGCATCTGCTCCATTGTCCAATTCT
NM_005254.5       ACATCAGTATTAGCTACATTAGCTGCCTTAGCTGAAGCATCTGCTCCATTGTCCAATTCT
NM_016654.4       ACATCAGTATTAGCTACATTAGCTGCCTTAGCTGAAGCATCTGCTCCATTGTCCAATTCT
                  ************************************************************

HsCD00329616      TCAGAAACTCCAGTAGTGGCCACAGAAGAAGTAGTTACTGCAGAATCTGTGGATGGTGCC
NM_005254.5       TCAGAAACTCCAGTAGTGGCCACAGAAGAAGTAGTTACTGCAGAATCTGTGGATGGTGCC
NM_016654.4       TCAGAAACTCCAGTAGTGGCCACAGAAGAAGTAGTTACTGCAGAATCTGTGGATGGTGCC
                  ************************************************************

HsCD00329616      ATTCAGCAAGTAGTTAGTTCAGGGGGTCAGCAAGTCATCGCAATAGTTACAGATGGAATT
NM_005254.5       ATTCAGCAAGTAGTTAGTTCAGGGGGTCAGCAAGTCATCACAATAGTTACAGATGGAATT
NM_016654.4       ATTCAGCAAGTAGTTAGTTCAGGGGGTCAGCAAGTCATCACAATAGTTACAGATGGAATT
                  ***************************************.********************

HsCD00329616      CAGCTTGGAAATTTGCACTCTATTCCAACCAGTGGAATTGGTCAGCCCATCATTGTGACC
NM_005254.5       CAGCTTGGAAATTTGCACTCTATTCCAACCAGTGGAATTGGTCAGCCCATCATTGTGACC
NM_016654.4       CAGCTTGGAAATTTGCACTCTATTCCAACCAGTGGAATTGGTCAGCCCATCATTGTGACC
                  ************************************************************

HsCD00329616      ATGCCAGATGGACAACAAGTATTAACAGTACCAGCAACAGACATTGCTGAAGAAACTGTT
NM_005254.5       ATGCCAGATGGACAACAAGTATTAACAGTACCAGCAACAGACATTGCTGAAGAAACTGTT
NM_016654.4       ATGCCAGATGGACAACAAGTATTAACAGTACCAGCAACAGACATTGCTGAAGAAACTGTT
                  ************************************************************

HsCD00329616      ATAAGTGAAGAACCACCAGCTAAGAGACAATGTATCGAAATAATTGAAAACCGGGTGGAA
NM_005254.5       ATAAGTGAAGAACCACCAGCTAAGAGACAATGTATCGAAATAATTGAAAACCGGGTGGAA
NM_016654.4       ATAAGTGAAGAACCACCAGCTAAGAGACAATGTATCGAAATAATTGAAAACCGGGTGGAA
                  ************************************************************

HsCD00329616      TCTGCAGAAATAGAAGAGAGAGAAGCTCTTCAGAAACAGCTGGATGAAGCAAATCGAGAA
NM_005254.5       TCTGCAGAAATAGAAGAGAGAGAAGCTCTTCAGAAACAGCTGGATGAAGCAAATCGAGAA
NM_016654.4       TCTGCAGAAATAGAAGAGAGAGAAGCTCTTCAGAAACAGCTGGATGAAGCAAATCGAGAA
                  ************************************************************

HsCD00329616      GCACAAAAATATCGACAGCAGCTCCTAAAGAAAGAACAGGAAGCAGAGGCCTACAGACAG
NM_005254.5       GCACAAAAATATCGACAGCAGCTCCTAAAGAAAGAACAGGAAGCAGAGGCCTACAGACAG
NM_016654.4       GCACAAAAATATCGACAGCAGCTCCTAAAGAAAGAACAGGAAGCAGAGGCCTACAGACAG
                  ************************************************************

HsCD00329616      AAGTTGGAAGCTATGACTCGTCTTCAGACTAATAAAGAAGCTGTTTTG
NM_005254.5       AAGTTGGAAGCTATGACTCGTCTTCAGACTAATAAAGAAGCTGTTTAA
NM_016654.4       AAGTTGGAAGCTATGACTCGTCTTCAGACTAATAAAGAAGCTGTTTAA
                  **********************************************:.

Pathways

Reactome : Mitochondrial biogenesis
Reactome : Organelle biogenesis and maintenance
Reactome : Transcriptional activation of mitochondrial biogenesis
WikiPathway : Mitochondrial Gene Expression
WikiPathway : Myometrial Relaxation and Contraction Pathways

Protein

SMART domain : ANK : ankyrin repeats
UniProt : Q06547
HPRD : 15975

Clone

Cloning Information : 269118
HIP Master Clone ID : 141489
Original Clone ID : FLH269118.01L

Organism


TAX_ID : 9606
Species Specific ID: 2553

Genome

Chromosome : 15
Map Location : 15q21.2
Ensembl : ENSG00000104064

Reagents

Labome : GABPB1-antibody