DNASU Plasmid Repository • 480.965.5697 | Email

OLIG3 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  OLIG3
Gene Name:  oligodendrocyte transcription factor 3
Sequence             
Original Clone ID: FLH268072.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329623

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 819nts         Open reading frame : 1 to 819
Reference Sequence Alignment

ATGAATTCTGATTCGAGCTCTGTCTCCAGCAGAGCTTCATCTCCGGACATGGATGAGATG
TACCTGAGGGACCACCACCACCGCCACCACCACCACCAGGAGAGCCGTCTCAACTCGGTC
TCGTCCACGCAGGGCGATATGATGCAGAAGATGCCCGGGGAAAGCCTCTCGCGGGCTGGC
GCCAAGGCCGCGGGAGAGAGCAGCAAGTACAAAATCAAGAAGCAGCTGTCGGAGCAGGAC
CTACAGCAGTTGAGGCTGAAGATCAACGGACGCGAACGCAAGCGGATGCACGACCTGAAC
CTAGCCATGGACGGGCTGCGCGAAGTCATGCCCTACGCGCATGGGCCGTCGGTGCGCAAG
CTCTCCAAGATCGCCACACTCCTGCTCGCCAGAAACTACATCCTCATGCTCACCAGCTCC
CTGGAGGAGATGAAGAGGCTGGTTGGCGAGATCTATGGGGGCCACCACTCGGCCTTTCAC
TGCGGGACCGTGGGCCACTCGGCCGGCCACCCCGCGCACGCGGCCAACTCCGTGCACCCG
GTGCACCCCATCTTGGGCGGCGCGCTCTCATCTGGCAACGCCTCGTCACCGCTGTCCGCC
GCCTCACTTCCCGCCATCGGCACCATCCGGCCTCCCCACTCGCTACTCAAGGCGCCCTCC
ACGCCGCCCGCGCTGCAGCTGGGCAGCGGCTTCCAGCACTGGGCTGGTCTGCCCTGCCCC
TGCACCATCTGCCAGATGCCGCCGCCGCCGCACCTGTCCGCTCTCTCCACAGCCAACATG
GCCCGGCTGTCGGCCGAGTCCAAGGACTTGCTCAAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1034
Start on reference sequence 216
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : OLIG3
Symbol Nomenclature : OLIG3
SYNONYM : Bhlhb7; bHLHe20
Designation : class B basic helix-loop-helix protein 7|class E basic helix-loop-helix protein 20|oligo3
Full Nomenclature : oligodendrocyte transcription factor 3
GENEID : 167826
Locus Tag : RP3-372K1.2
HGNC : 18003
MIM : 609323
Vega : OTTHUMG00000015657
Target GenBank: BC051352

 

Reference Sequence Alignment

  

HsCD00329623      ATGAATTCTGATTCGAGCTCTGTCTCCAGCAGAGCTTCATCTCCGGACATGGATGAGATG
NM_175747.2       ATGAATTCTGATTCGAGCTCTGTCTCCAGCAGAGCTTCATCTCCGGACATGGATGAGATG
                  ************************************************************

HsCD00329623      TACCTGAGGGACCACCACCACCGCCACCACCACCACCAGGAGAGCCGTCTCAACTCGGTC
NM_175747.2       TACCTGAGGGACCACCACCACCGCCACCACCACCACCAGGAGAGCCGTCTCAACTCGGTC
                  ************************************************************

HsCD00329623      TCGTCCACGCAGGGCGATATGATGCAGAAGATGCCCGGGGAAAGCCTCTCGCGGGCTGGC
NM_175747.2       TCGTCCACGCAGGGCGATATGATGCAGAAGATGCCCGGGGAAAGCCTCTCGCGGGCTGGC
                  ************************************************************

HsCD00329623      GCCAAGGCCGCGGGAGAGAGCAGCAAGTACAAAATCAAGAAGCAGCTGTCGGAGCAGGAC
NM_175747.2       GCCAAGGCCGCGGGAGAGAGCAGCAAGTACAAAATCAAGAAGCAGCTGTCGGAGCAGGAC
                  ************************************************************

HsCD00329623      CTACAGCAGTTGAGGCTGAAGATCAACGGACGCGAACGCAAGCGGATGCACGACCTGAAC
NM_175747.2       CTACAGCAGTTGAGGCTGAAGATCAACGGACGCGAACGCAAGCGGATGCACGACCTGAAC
                  ************************************************************

HsCD00329623      CTAGCCATGGACGGGCTGCGCGAAGTCATGCCCTACGCGCATGGGCCGTCGGTGCGCAAG
NM_175747.2       CTAGCCATGGACGGGCTGCGCGAAGTCATGCCCTACGCGCATGGGCCGTCGGTGCGCAAG
                  ************************************************************

HsCD00329623      CTCTCCAAGATCGCCACACTCCTGCTCGCCAGAAACTACATCCTCATGCTCACCAGCTCC
NM_175747.2       CTCTCCAAGATCGCCACACTCCTGCTCGCCAGAAACTACATCCTCATGCTCACCAGCTCC
                  ************************************************************

HsCD00329623      CTGGAGGAGATGAAGAGGCTGGTTGGCGAGATCTATGGGGGCCACCACTCGGCCTTTCAC
NM_175747.2       CTGGAGGAGATGAAGAGGCTGGTTGGCGAGATCTATGGGGGCCACCACTCGGCCTTTCAC
                  ************************************************************

HsCD00329623      TGCGGGACCGTGGGCCACTCGGCCGGCCACCCCGCGCACGCGGCCAACTCCGTGCACCCG
NM_175747.2       TGCGGGACCGTGGGCCACTCGGCCGGCCACCCCGCGCACGCGGCCAACTCCGTGCACCCG
                  ************************************************************

HsCD00329623      GTGCACCCCATCTTGGGCGGCGCGCTCTCATCTGGCAACGCCTCGTCACCGCTGTCCGCC
NM_175747.2       GTGCACCCCATCTTGGGCGGCGCGCTCTCATCTGGCAACGCCTCGTCACCGCTGTCCGCC
                  ************************************************************

HsCD00329623      GCCTCACTTCCCGCCATCGGCACCATCCGGCCTCCCCACTCGCTACTCAAGGCGCCCTCC
NM_175747.2       GCCTCACTTCCCGCCATCGGCACCATCCGGCCTCCCCACTCGCTACTCAAGGCGCCCTCC
                  ************************************************************

HsCD00329623      ACGCCGCCCGCGCTGCAGCTGGGCAGCGGCTTCCAGCACTGGGCTGGTCTGCCCTGCCCC
NM_175747.2       ACGCCGCCCGCGCTGCAGCTGGGCAGCGGCTTCCAGCACTGGGCTGGTCTGCCCTGCCCC
                  ************************************************************

HsCD00329623      TGCACCATCTGCCAGATGCCGCCGCCGCCGCACCTGTCCGCTCTCTCCACAGCCAACATG
NM_175747.2       TGCACCATCTGCCAGATGCCGCCGCCGCCGCACCTGTCCGCTCTCTCCACAGCCAACATG
                  ************************************************************

HsCD00329623      GCCCGGCTGTCGGCCGAGTCCAAGGACTTGCTCAAGTTG
NM_175747.2       GCCCGGCTGTCGGCCGAGTCCAAGGACTTGCTCAAGTGA
                  ************************************* .

Protein

SMART domain : HLH : helix loop helix domain
UniProt : Q7RTU3
HPRD : 17662

Clone

Cloning Information : 268072
HIP Master Clone ID : 193102
Original Clone ID : FLH268072.01L

Organism


TAX_ID : 9606
Species Specific ID: 167826

Genome

Chromosome : 6
Map Location : 6q23.3
Ensembl : ENSG00000177468

Reagents

Labome : OLIG3-antibody