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PHOX2A (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PHOX2A
Gene Name:  paired-like homeobox 2a
Sequence             
Original Clone ID: FLH268078.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329629

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 855nts         Open reading frame : 1 to 855
Reference Sequence Alignment

ATGGACTACTCCTACCTCAATTCGTACGACTCGTGCGTGGCGGCCATGGAGGCGTCCGCC
TACGGCGACTTTGGCGCCTGCAGCCAGCCCGGCGGCTTCCAATACAGCCCCCTGCGGCCC
GCTTTCCCCGCGGCAGGGCCGCCCTGCCCCGCGCTCGGCTCCTCCAACTGCGCACTTGGC
GCCCTACGCGACCACCAGCCCGCGCCCTACTCGGCAGTGCCCTACAAGTTCTTCCCAGAG
CCATCCGGCCTGCACGAGAAGCGCAAGCAGCGGCGCATCCGCACCACGTTCACCAGCGCG
CAGCTCAAGGAGCTGGAGCGCGTTTTCGCTGAGACCCACTACCCCGACATTTACACGCGT
GAGGAGCTGGCGCTCAAGATCGACCTCACTGAGGCTCGCGTGCAGGTCTGGTTCCAGAAC
CGCCGGGCCAAGTTCCGCAAACAGGAGCGCGCGGCCAGCGCCAAGGGCGCGGCGGGCGCG
GCGGGCGCCAAAAAGGGCGAGGCGCGCTGCTCCTCCGAGGACGACGATTCCAAGGAGTCC
ACGTGCAGCCCCACGCCCGATAGCACCGCCTCGCTGCCGCCGCCGCCTGCGCCCGGCCTG
GCCAGCCCGCGCCTGAGCCCCAGCCCGCTGCCCGTCGCACTGGGCTCCGGGCCGGGACCT
GGGCCGGGGCCACAGCCGCTCAAGGGCGCACTGTGGGCCGGTGTGGCGGGCGGTGGGGGC
GGCGGGCCTGGCGCGGGAGCGGCCGAACTACTTAAGGCTTGGCAGCCGGCGGAGTCCGGC
CCCGGGCCCTTCTCCGGGGTTCTGTCCTCCTTTCACCGGAAGCCCGGCCCCGCCCTGAAG
ACCAATCTCTTCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1009
Start on reference sequence 155
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PHOX2A
Symbol Nomenclature : PHOX2A
SYNONYM : ARIX; CFEOM2; FEOM2; NCAM2; PMX2A
Designation : ARIX1 homeodomain protein|aristaless homeobox homolog|aristaless homeobox protein homolog|arix homeodomain protein|paired mesoderm homeobox protein 2A
Full Nomenclature : paired-like homeobox 2a
GENEID : 401
HGNC : 691
MIM : 602753
Vega : OTTHUMG00000167899
Target GenBank: BC041564

 

Reference Sequence Alignment

  

HsCD00329629      ATGGACTACTCCTACCTCAATTCGTACGACTCGTGCGTGGCGGCCATGGAGGCGTCCGCC
NM_005169.3       ATGGACTACTCCTACCTCAATTCGTACGACTCGTGCGTGGCGGCCATGGAGGCGTCCGCC
                  ************************************************************

HsCD00329629      TACGGCGACTTTGGCGCCTGCAGCCAGCCCGGCGGCTTCCAATACAGCCCCCTGCGGCCC
NM_005169.3       TACGGCGACTTTGGCGCCTGCAGCCAGCCCGGCGGCTTCCAATACAGCCCCCTGCGGCCC
                  ************************************************************

HsCD00329629      GCTTTCCCCGCGGCAGGGCCGCCCTGCCCCGCGCTCGGCTCCTCCAACTGCGCACTTGGC
NM_005169.3       GCTTTCCCCGCGGCAGGGCCGCCCTGCCCCGCGCTCGGCTCCTCCAACTGCGCACTTGGC
                  ************************************************************

HsCD00329629      GCCCTACGCGACCACCAGCCCGCGCCCTACTCGGCAGTGCCCTACAAGTTCTTCCCAGAG
NM_005169.3       GCCCTACGCGACCACCAGCCCGCGCCCTACTCGGCAGTGCCCTACAAGTTCTTCCCAGAG
                  ************************************************************

HsCD00329629      CCATCCGGCCTGCACGAGAAGCGCAAGCAGCGGCGCATCCGCACCACGTTCACCAGCGCG
NM_005169.3       CCATCCGGCCTGCACGAGAAGCGCAAGCAGCGGCGCATCCGCACCACGTTCACCAGCGCG
                  ************************************************************

HsCD00329629      CAGCTCAAGGAGCTGGAGCGCGTTTTCGCTGAGACCCACTACCCCGACATTTACACGCGT
NM_005169.3       CAGCTCAAGGAGCTGGAGCGCGTTTTCGCTGAGACCCACTACCCCGACATTTACACGCGT
                  ************************************************************

HsCD00329629      GAGGAGCTGGCGCTCAAGATCGACCTCACTGAGGCTCGCGTGCAGGTCTGGTTCCAGAAC
NM_005169.3       GAGGAGCTGGCGCTCAAGATCGACCTCACTGAGGCTCGCGTGCAGGTCTGGTTCCAGAAC
                  ************************************************************

HsCD00329629      CGCCGGGCCAAGTTCCGCAAACAGGAGCGCGCGGCCAGCGCCAAGGGCGCGGCGGGCGCG
NM_005169.3       CGCCGGGCCAAGTTCCGCAAACAGGAGCGCGCGGCCAGCGCCAAGGGCGCGGCGGGCGCG
                  ************************************************************

HsCD00329629      GCGGGCGCCAAAAAGGGCGAGGCGCGCTGCTCCTCCGAGGACGACGATTCCAAGGAGTCC
NM_005169.3       GCGGGCGCCAAAAAGGGCGAGGCGCGCTGCTCCTCCGAGGACGACGATTCCAAGGAGTCC
                  ************************************************************

HsCD00329629      ACGTGCAGCCCCACGCCCGATAGCACCGCCTCGCTGCCGCCGCCGCCTGCGCCCGGCCTG
NM_005169.3       ACGTGCAGCCCCACGCCCGATAGCACCGCCTCGCTGCCGCCGCCGCCTGCGCCCGGCCTG
                  ************************************************************

HsCD00329629      GCCAGCCCGCGCCTGAGCCCCAGCCCGCTGCCCGTCGCACTGGGCTCCGGGCCGGGACCT
NM_005169.3       GCCAGCCCGCGCCTGAGCCCCAGCCCGCTGCCCGTCGCACTGGGCTCCGGGCCGGGACCT
                  ************************************************************

HsCD00329629      GGGCCGGGGCCACAGCCGCTCAAGGGCGCACTGTGGGCCGGTGTGGCGGGCGGTGGGGGC
NM_005169.3       GGGCCGGGGCCACAGCCGCTCAAGGGCGCACTGTGGGCCGGTGTGGCGGGCGGTGGGGGC
                  ************************************************************

HsCD00329629      GGCGGGCCTGGCGCGGGAGCGGCCGAACTACTTAAGGCTTGGCAGCCGGCGGAGTCCGGC
NM_005169.3       GGCGGGCCTGGCGCGGGAGCGGCCGAACTACTTAAGGCTTGGCAGCCGGCGGAGTCCGGC
                  ************************************************************

HsCD00329629      CCCGGGCCCTTCTCCGGGGTTCTGTCCTCCTTTCACCGGAAGCCCGGCCCCGCCCTGAAG
NM_005169.3       CCCGGGCCCTTCTCCGGGGTTCTGTCCTCCTTTCACCGGAAGCCCGGCCCCGCCCTGAAG
                  ************************************************************

HsCD00329629      ACCAATCTCTTCTTG
NM_005169.3       ACCAATCTCTTCTAG
                  *************:*

Pathways

WikiPathway : SIDS Susceptibility Pathways

Protein

SMART domain : HOX : Homeodomain
UniProt : O14813
HPRD : 04129

Clone

Cloning Information : 268078
HIP Master Clone ID : 193109
Original Clone ID : FLH268078.01L

Organism


TAX_ID : 9606
Species Specific ID: 401

Genome

Chromosome : 11
Map Location : 11q13.2
Ensembl : ENSG00000165462

Reagents

Labome : PHOX2A-antibody