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ZNF530 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF530
Gene Name:  zinc finger protein 530
Sequence             
Original Clone ID: FLH268087.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329638

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1800nts         Open reading frame : 1 to 1800
Reference Sequence Alignment

ATGGCGGCGGCACTGAGGGCCCCGACCCAGCAGGTTTTTGTAGCCTTTGAGGATGTGGCC
ATTTACTTCTCCCAGGAGGAGTGGGAGCTCCTTGATGAGATGCAGAGGCTCCTGTACCGC
GATGTGATGCTGGAGAACTTTGCAGTTATGGCATCCCTAGGTTGCTGGTGTGGAGCAGTA
GATGAGGGGACGCCTTCTGCAGAGAGCGTTTCTGTGGAAGAACTGTCACAGGGCAGGACT
CCAAAGGCAGATACATCCACTGATAAGAGTCACCCCTGTGAGATTTGTACCCCAGTCCTG
AGAGACATTTTACAAATGATTGAGCTCCAAGCCTCACCCTGTGGACAGAAATTGTACTTG
GGTGGAGCACCAAGAGATTTCTGGATGAGTTCAAACCTTCACCAGCTCCAGAAGCTTGAT
AATGGAGAGAAGCTCTTTAAAGTGGATGGGGACCAGGCCTCATTTATGATGAACTGCAGG
TTCCATGTGTCAGGAAAACCCTTCACGTTTGGGGAAGTCGGGAGGGACTTTTCAGCCACC
TCAGGACTTCTCCAGCATCAGGTGACTCCCACCATTGAGAGACCACACAGCAGGATTAGA
CACTTGAGAGTTCCCACTGGACGAAAGCCTCTCAAATACACTGAATCCAGGAAATCTTTT
AGAGAGAAATCTGTATTCATTCAACACCAAAGAGCTGACTCTGGAGAAAGGCCTTACAAG
TGCAGTGAATGTGGGAAATCCTTTAGTCAAAGTTCTGGCTTTCTTCGACACAGGAAAGCA
CACGGTAGAACAAGGACTCATGAATGTAGTGAATGTGGGAAATCATTTAGTCGCAAAACT
CACCTAACTCAACACCAAAGAGTTCACACTGGAGAAAGGCCTTATGACTGCAGTGAATGT
GGCAAATCCTTTCGCCAGGTATCTGTCCTCATTCAACATCAACGAGTTCACACTGGAGAA
AGGCCTTATGAGTGCAGTGAATGTGGGAAATCTTTTAGCCACAGCACTAACCTCTATCGT
CACAGGAGTGCCCACACTAGCACAAGGCCTTATGAGTGCAGTGAATGTGGAAAATCCTTT
AGCCATAGCACTAACCTCTTTCGACACTGGAGAGTTCACACTGGAGTAAGGCCTTATGAG
TGTAGTGAATGTGGGAAAGCATTTAGTTGCAATATCTACCTTATTCACCACCAAAGATTT
CACACTGGAGAAAGACCTTATGTGTGCAGTGAATGTGGGAAATCATTTGGCCAGAAATCT
GTCCTCATTCAACACCAAAGAGTTCACACTGGAGAAAGGCCTTATGAGTGCAGTGAATGT
GGGAAAGTTTTTAGCCAAAGCTCTGGCCTCTTTCGACACAGAAGAGCTCACACTAAAACA
AAGCCTTATGAGTGCAGTGAATGTGAAAAATCATTTAGTTGCAAAACTGACCTCATTCGA
CACCAGACAGTTCACACTGGAGAAAGGCCTTATGAGTGCAGTGTATGTGGGAAATCTTTT
ATCCGAAAAACCCACCTCATTCGACACCAGACTGTTCACACTAATGAAAGGCCTTATGAG
TGCGATGAATGTGGGAAATCCTATAGCCAAAGCTCTGCCCTCCTTCAGCATAGGAGAGTT
CACACTGGAGAAAGGCCTTATGAGTGCAGAGAATGTGGGAAATCTTTTACCCGCAAAAAT
CACCTCATTCAACACAAGACAGTTCACACTGGAGAAAGGCCTTATGAATGCAGTGAATGT
GGAAAATCCTTTAGCCAAAGCTCTGGCCTCTTAAGACACAGAAGAGTTCATGTGCAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2020
Start on reference sequence 221
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF530
Symbol Nomenclature : ZNF530
Full Nomenclature : zinc finger protein 530
GENEID : 348327
HGNC : 29297
Vega : OTTHUMG00000183469
Target GenBank: BC060865

 

Reference Sequence Alignment

  

HsCD00329638      ATGGCGGCGGCACTGAGGGCCCCGACCCAGCAGGTTTTTGTAGCCTTTGAGGATGTGGCC
NM_020880.3       ATGGCGGCGGCACTGAGGGCCCCGACCCAGCAGGTTTTTGTAGCCTTTGAGGATGTGGCC
                  ************************************************************

HsCD00329638      ATTTACTTCTCCCAGGAGGAGTGGGAGCTCCTTGATGAGATGCAGAGGCTCCTGTACCGC
NM_020880.3       ATTTACTTCTCCCAGGAGGAGTGGGAGCTCCTTGATGAGATGCAGAGGCTCCTGTACCGC
                  ************************************************************

HsCD00329638      GATGTGATGCTGGAGAACTTTGCAGTTATGGCATCCCTAGGTTGCTGGTGTGGAGCAGTA
NM_020880.3       GATGTGATGCTGGAGAACTTTGCAGTTATGGCATCCCTAGGTTGCTGGTGTGGAGCAGTA
                  ************************************************************

HsCD00329638      GATGAGGGGACGCCTTCTGCAGAGAGCGTTTCTGTGGAAGAACTGTCACAGGGCAGGACT
NM_020880.3       GATGAGGGGACGCCTTCTGCAGAGAGCGTTTCTGTGGAAGAACTGTCACAGGGCAGGACT
                  ************************************************************

HsCD00329638      CCAAAGGCAGATACATCCACTGATAAGAGTCACCCCTGTGAGATTTGTACCCCAGTCCTG
NM_020880.3       CCAAAGGCAGATACATCCACTGATAAGAGTCACCCCTGTGAGATTTGTACCCCAGTCCTG
                  ************************************************************

HsCD00329638      AGAGACATTTTACAAATGATTGAGCTCCAAGCCTCACCCTGTGGACAGAAATTGTACTTG
NM_020880.3       AGAGACATTTTACAAATGATTGAGCTCCATGCCTCACCCTGTGGACAGAAATTGTACTTG
                  *****************************:******************************

HsCD00329638      GGTGGAGCACCAAGAGATTTCTGGATGAGTTCAAACCTTCACCAGCTCCAGAAGCTTGAT
NM_020880.3       GGTGGAGCATCAAGAGATTTCTGGATGAGTTCAAACCTTCACCAGCTCCAGAAGCTTGAT
                  ********* **************************************************

HsCD00329638      AATGGAGAGAAGCTCTTTAAAGTGGATGGGGACCAGGCCTCATTTATGATGAACTGCAGG
NM_020880.3       AATGGAGAGAAGCTCTTTAAAGTGGATGGGGACCAGGCCTCATTTATGATGAACTGCAGG
                  ************************************************************

HsCD00329638      TTCCATGTGTCAGGAAAACCCTTCACGTTTGGGGAAGTCGGGAGGGACTTTTCAGCCACC
NM_020880.3       TTCCATGTGTCAGGAAAACCCTTCACGTTTGGGGAAGTCGGGAGGGACTTTTCAGCCACC
                  ************************************************************

HsCD00329638      TCAGGACTTCTCCAGCATCAGGTGACTCCCACCATTGAGAGACCACACAGCAGGATTAGA
NM_020880.3       TCAGGACTTCTCCAGCATCAGGTGACTCCCACCATTGAGAGACCACACAGCAGGATTAGA
                  ************************************************************

HsCD00329638      CACTTGAGAGTTCCCACTGGACGAAAGCCTCTCAAATACACTGAATCCAGGAAATCTTTT
NM_020880.3       CACTTGAGAGTTCCCACTGGACGAAAGCCTCTCAAATACACTGAATCCAGGAAATCTTTT
                  ************************************************************

HsCD00329638      AGAGAGAAATCTGTATTCATTCAACACCAAAGAGCTGACTCTGGAGAAAGGCCTTACAAG
NM_020880.3       AGAGAGAAATCTGTATTCATTCAACACCAAAGAGCTGACTCTGGAGAAAGGCCTTACAAG
                  ************************************************************

HsCD00329638      TGCAGTGAATGTGGGAAATCCTTTAGTCAAAGTTCTGGCTTTCTTCGACACAGGAAAGCA
NM_020880.3       TGCAGTGAATGTGGGAAATCCTTTAGTCAAAGTTCTGGCTTTCTTCGACACAGGAAAGCA
                  ************************************************************

HsCD00329638      CACGGTAGAACAAGGACTCATGAATGTAGTGAATGTGGGAAATCATTTAGTCGCAAAACT
NM_020880.3       CACGGTAGAACAAGGACTCATGAATGTAGTGAATGTGGGAAATCATTTAGTCGCAAAACT
                  ************************************************************

HsCD00329638      CACCTAACTCAACACCAAAGAGTTCACACTGGAGAAAGGCCTTATGACTGCAGTGAATGT
NM_020880.3       CACCTAACTCAACACCAAAGAGTTCACACTGGAGAAAGGCCTTATGACTGCAGTGAATGT
                  ************************************************************

HsCD00329638      GGCAAATCCTTTCGCCAGGTATCTGTCCTCATTCAACATCAACGAGTTCACACTGGAGAA
NM_020880.3       GGCAAATCCTTTCGCCAGGTATCTGTCCTCATTCAACATCAACGAGTTCACACTGGAGAA
                  ************************************************************

HsCD00329638      AGGCCTTATGAGTGCAGTGAATGTGGGAAATCTTTTAGCCACAGCACTAACCTCTATCGT
NM_020880.3       AGGCCTTATGAGTGCAGTGAATGTGGGAAATCTTTTAGCCACAGCACTAACCTCTATCGT
                  ************************************************************

HsCD00329638      CACAGGAGTGCCCACACTAGCACAAGGCCTTATGAGTGCAGTGAATGTGGAAAATCCTTT
NM_020880.3       CACAGGAGTGCCCACACTAGCACAAGGCCTTATGAGTGCAGTGAATGTGGAAAATCCTTT
                  ************************************************************

HsCD00329638      AGCCATAGCACTAACCTCTTTCGACACTGGAGAGTTCACACTGGAGTAAGGCCTTATGAG
NM_020880.3       AGCCATAGCACTAACCTCTTTCGACACTGGAGAGTTCACACTGGAGTAAGGCCTTATGAG
                  ************************************************************

HsCD00329638      TGTAGTGAATGTGGGAAAGCATTTAGTTGCAATATCTACCTTATTCACCACCAAAGATTT
NM_020880.3       TGTAGTGAATGTGGGAAAGCATTTAGTTGCAATATCTACCTTATTCACCACCAAAGATTT
                  ************************************************************

HsCD00329638      CACACTGGAGAAAGACCTTATGTGTGCAGTGAATGTGGGAAATCATTTGGCCAGAAATCT
NM_020880.3       CACACTGGAGAAAGACCTTATGTGTGCAGTGAATGTGGGAAATCATTTGGCCAGAAATCT
                  ************************************************************

HsCD00329638      GTCCTCATTCAACACCAAAGAGTTCACACTGGAGAAAGGCCTTATGAGTGCAGTGAATGT
NM_020880.3       GTCCTCATTCAACACCAAAGAGTTCACACTGGAGAAAGGCCTTATGAGTGCAGTGAATGT
                  ************************************************************

HsCD00329638      GGGAAAGTTTTTAGCCAAAGCTCTGGCCTCTTTCGACACAGAAGAGCTCACACTAAAACA
NM_020880.3       GGGAAAGTTTTTAGCCAAAGCTCTGGCCTCTTTCGACACAGAAGAGCTCACACTAAAACA
                  ************************************************************

HsCD00329638      AAGCCTTATGAGTGCAGTGAATGTGAAAAATCATTTAGTTGCAAAACTGACCTCATTCGA
NM_020880.3       AAGCCTTATGAGTGCAGTGAATGTGAAAAATCATTTAGTTGCAAAACTGACCTCATTCGA
                  ************************************************************

HsCD00329638      CACCAGACAGTTCACACTGGAGAAAGGCCTTATGAGTGCAGTGTATGTGGGAAATCTTTT
NM_020880.3       CACCAGACAGTTCACACTGGAGAAAGGCCTTATGAGTGCAGTGTATGTGGGAAATCTTTT
                  ************************************************************

HsCD00329638      ATCCGAAAAACCCACCTCATTCGACACCAGACTGTTCACACTAATGAAAGGCCTTATGAG
NM_020880.3       ATCCGAAAAACCCACCTCATTCGACACCAGACTGTTCACACTAATGAAAGGCCTTATGAG
                  ************************************************************

HsCD00329638      TGCGATGAATGTGGGAAATCCTATAGCCAAAGCTCTGCCCTCCTTCAGCATAGGAGAGTT
NM_020880.3       TGCGATGAATGTGGGAAATCCTATAGCCAAAGCTCTGCCCTCCTTCAGCATAGGAGAGTT
                  ************************************************************

HsCD00329638      CACACTGGAGAAAGGCCTTATGAGTGCAGAGAATGTGGGAAATCTTTTACCCGCAAAAAT
NM_020880.3       CACACTGGAGAAAGGCCTTATGAGTGCAGAGAATGTGGGAAATCTTTTACCCGCAAAAAT
                  ************************************************************

HsCD00329638      CACCTCATTCAACACAAGACAGTTCACACTGGAGAAAGGCCTTATGAATGCAGTGAATGT
NM_020880.3       CACCTCATTCAACACAAGACAGTTCACACTGGAGAAAGGCCTTATGAATGCAGTGAATGT
                  ************************************************************

HsCD00329638      GGAAAATCCTTTAGCCAAAGCTCTGGCCTCTTAAGACACAGAAGAGTTCATGTGCAGTTG
NM_020880.3       GGAAAATCCTTTAGCCAAAGCTCTGGCCTCTTAAGACACAGAAGAGTTCATGTGCAGTGA
                  ********************************************************** .

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
SMART domain : KRAB : krueppel associated box
UniProt : Q6P9A1
HPRD : 15822

Clone

Cloning Information : 268087
HIP Master Clone ID : 193120
Original Clone ID : FLH268087.01L

Organism


TAX_ID : 9606
Species Specific ID: 348327

Genome

Chromosome : 19
Map Location : 19q13.43
Ensembl : ENSG00000183647

Reagents

Labome : ZNF530-antibody