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ZNF221 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF221
Gene Name:  zinc finger protein 221
Sequence             
Original Clone ID: FLH268093.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329644

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1854nts         Open reading frame : 1 to 1854
Reference Sequence Alignment

ATGATTTCACCTTCACTTGAACTGCTTCATTCAGGACTCTGCAAATTCCCTGAAGTAGAA
GGAAAAATGACCACATTCAAAGAGGCAGTGACATTCAAGGATGTGGCTGTGGTCTTCACT
GAGGAGGAGCTGGGGCTGCTGGACCCTGCCCAGAGGAAGCTGTACCGAGATGTGATGCTA
GAGAACTTCAGGAACCTGCTCTCAGTAGGGAATCAACCATTCCACCAAGATACTTTCCAC
TTCTTAGGGAAGGAAAAGTTTTGGAAGATGAAGACAACAAGCCAAAGAGAAGGGAATTCA
GGAGGCAAGATCCAAATTGAGATGGAGACTGTTCCAGAAGCAGGACCACATGAAGAGTGG
TCCTGTCAGCAAATATGGGAACAAATTGCAAGTGACCTAACCAGGTCTCAAAACTCCATA
AGGAACAGCTCTCAGTTCTTCAAAGAAGGTGATGTCCCCTGCCAGATTGAGGCAAGACTA
TCTATAAGTCACGTGCAACAGAAACCTTACCGTTGTAATGAATGTAAACAGTCCATCAGT
GATGTTTCTGTCTTTGATCTTCATCAACAATCACACTCAGGAGAGAAATCTCATACATGT
GGTGAGTGTGGAAAAAGCTTCTGTTACAGCCCAGCCCTTCATATTCATCAGAGAGTCCAT
ATGGGAGAAAAATGCTATAAGTGTGATGTGTGTGGTAAGGAATTTAATCAGAGCTCACAT
CTGCAAACTCATCAGAGAGTCCATACTGGAGAGAAACCATTCAAACGTGGGCAATGTGGG
AAAGGCTTCCATAGTAGATCAGCACTTAATGTTCATTGCAAATTGCACACAGGAGAGAAA
CCTTATAATTGTGAGGAATGTGGGAAAGCCTTCATTCATGATTCACAGCTTCAGGAACAT
CAGAGAATCCATACTGGGGAGAAGCCATTCAAATGTGATATATGTGGTAAGAGCTTCCGT
GTTAGATCAAGACTTAATAGGCATTCCATGGTTCACACAGGAGAAAAACCATTCAGATGT
GATACATGTGGCAAGAACTTTCGTCAGAGATCAGCACTTAATAGTCATTCCATGGTCCAC
ATAGAAGAGAAGCCATACAAATGTGAGCAATGTGGAAAAGGCTTCATTTGTAGGCGAGAT
TTTTGTAAGCATCAGATGGTCCACACAGGAGAGAAACCATATAATTGTAAAGAATGTGGG
AAGACCTTCAGATGGTCCTCATGTCTTTTGAACCATCAGCAAGTCCACAGTGGACAAAAA
TCCTTCAAATGTGAAGAATGTGGGAAGGGATTTTATACAAATTCACGACGATCTTCCCAT
CAGAGATCCCACAATGGAGAAAAGCCATATAACTGTGAGGAGTGTGGTAAGGACTATAAA
AGGAGGTTGGATCTTGAGTTTCACCAGAGGGTCCACACGGGTGAGAGACCCTATAATTGT
AAGGAATGTGGCAAGAGCTTTGGCTGGGCCTCCTGTCTTTTGAAACATCAGAGACTCCAC
AGTGGGGAAAAACCTTTCAAATGTGAAGAGTGTGGAAAGAGATTTACTCAGAGTACACAA
CTTCATTCCCATCAGACATGCCATACTGGAGAAAAGCTATACAAATGTGAGCAGTGTGAG
AAGGGGTACAACAGTAAATTTAATCTTGACATGCACCAGAGGGTCCACAGGGGAGAGCGA
CCCTATAATTGTAAGGAATGTGGAAAGAGCTTTGGCTGGGCTTCATGTCTTTTGAAACAT
CAGAGACTCCACAGTGGAGAAAAGCCATTGAAATCTGGAGTGTGGGAAGAGATCTACTCA
GAATTCACAGCTTCATTTACATCAGTAAGTCTATGTGGGAGAAAAGCCATATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1937
Start on reference sequence 84
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF221
Symbol Nomenclature : ZNF221
Full Nomenclature : zinc finger protein 221
GENEID : 7638
HGNC : 13014
Vega : OTTHUMG00000182214
Target GenBank: BC111981

 

Reference Sequence Alignment

  

HsCD00329644      ATGATTTCACCTTCACTTGAACTGCTTCATTCAGGACTCTGCAAATTCCCTGAAGTAGAA
NM_013359.2       ATGATTTCACCTTCACTTGAACTGCTTCATTCAGGACTCTGCAAATTCCCTGAAGTAGAA
                  ************************************************************

HsCD00329644      GGAAAAATGACCACATTCAAAGAGGCAGTGACATTCAAGGATGTGGCTGTGGTCTTCACT
NM_013359.2       GGAAAAATGACCACATTCAAAGAGGCAGTGACATTCAAGGATGTGGCTGTGGTCTTCACT
                  ************************************************************

HsCD00329644      GAGGAGGAGCTGGGGCTGCTGGACCCTGCCCAGAGGAAGCTGTACCGAGATGTGATGCTA
NM_013359.2       GAGGAGGAGCTGGGGCTGCTGGACCCTGCCCAGAGGAAGCTGTACCGAGATGTGATGCTA
                  ************************************************************

HsCD00329644      GAGAACTTCAGGAACCTGCTCTCAGTAGGGAATCAACCATTCCACCAAGATACTTTCCAC
NM_013359.2       GAGAACTTCAGGAACCTGCTCTCAGTAGGGAATCAACCATTCCACCAAGATACTTTCCAC
                  ************************************************************

HsCD00329644      TTCTTAGGGAAGGAAAAGTTTTGGAAGATGAAGACAACAAGCCAAAGAGAAGGGAATTCA
NM_013359.2       TTCTTAGGGAAGGAAAAGTTTTGGAAGATGAAGACAACAAGCCAAAGAGAAGGGAATTCA
                  ************************************************************

HsCD00329644      GGAGGCAAGATCCAAATTGAGATGGAGACTGTTCCAGAAGCAGGACCACATGAAGAGTGG
NM_013359.2       GGAGGCAAGATCCAAATTGAGATGGAGACTGTTCCAGAAGCAGGACCACATGAAGAGTGG
                  ************************************************************

HsCD00329644      TCCTGTCAGCAAATATGGGAACAAATTGCAAGTGACCTAACCAGGTCTCAAAACTCCATA
NM_013359.2       TCCTGTCAGCAAATATGGGAACAAATTGCAAGTGACCTAACCAGGTCTCAAAACTCCATA
                  ************************************************************

HsCD00329644      AGGAACAGCTCTCAGTTCTTCAAAGAAGGTGATGTCCCCTGCCAGATTGAGGCAAGACTA
NM_013359.2       AGGAACAGCTCTCAGTTCTTCAAAGAAGGTGATGTCCCCTGCCAGATTGAGGCAAGACTA
                  ************************************************************

HsCD00329644      TCTATAAGTCACGTGCAACAGAAACCTTACCGTTGTAATGAATGTAAACAGTCCATCAGT
NM_013359.2       TCTATAAGTCACGTGCAACAGAAACCTTACCGTTGTAATGAATGTAAACAGTCCTTCAGT
                  ******************************************************:*****

HsCD00329644      GATGTTTCTGTCTTTGATCTTCATCAACAATCACACTCAGGAGAGAAATCTCATACATGT
NM_013359.2       GATGTTTCTGTCTTTGATCTTCATCAACAATCACACTCAGGAGAGAAATCTCATACATGT
                  ************************************************************

HsCD00329644      GGTGAGTGTGGAAAAAGCTTCTGTTACAGCCCAGCCCTTCATATTCATCAGAGAGTCCAT
NM_013359.2       GGTGAGTGTGGAAAAAGCTTCTGTTACAGCCCAGCCCTTCATATTCATCAGAGAGTCCAT
                  ************************************************************

HsCD00329644      ATGGGAGAAAAATGCTATAAGTGTGATGTGTGTGGTAAGGAATTTAATCAGAGCTCACAT
NM_013359.2       ATGGGAGAAAAATGCTATAAGTGTGATGTGTGTGGTAAGGAATTTAATCAGAGCTCACAT
                  ************************************************************

HsCD00329644      CTGCAAACTCATCAGAGAGTCCATACTGGAGAGAAACCATTCAAACGTGGGCAATGTGGG
NM_013359.2       CTGCAAACTCATCAGAGAGTCCATACTGGAGAGAAACCATTCAAATGTGGGCAATGTGGG
                  ********************************************* **************

HsCD00329644      AAAGGCTTCCATAGTAGATCAGCACTTAATGTTCATTGCAAATTGCACACAGGAGAGAAA
NM_013359.2       AAAGGCTTCCATAGTAGATCAGCACTTAATGTTCATTGCAAATTGCACACAGGAGAGAAA
                  ************************************************************

HsCD00329644      CCTTATAATTGTGAGGAATGTGGGAAAGCCTTCATTCATGATTCACAGCTTCAGGAACAT
NM_013359.2       CCTTATAATTGTGAGGAATGTGGGAAAGCCTTCATTCATGATTCACAGCTTCAAGAACAT
                  *****************************************************.******

HsCD00329644      CAGAGAATCCATACTGGGGAGAAGCCATTCAAATGTGATATATGTGGTAAGAGCTTCCGT
NM_013359.2       CAGAGAATCCATACTGGGGAGAAGCCATTCAAATGTGATATATGTGGTAAGAGCTTCCGT
                  ************************************************************

HsCD00329644      GTTAGATCAAGACTTAATAGGCATTCCATGGTTCACACAGGAGAAAAACCATTCAGATGT
NM_013359.2       GTTAGATCAAGACTTAATAGGCATTCCATGGTTCACACAGGAGAAAAACCATTCAGATGT
                  ************************************************************

HsCD00329644      GATACATGTGGCAAGAACTTTCGTCAGAGATCAGCACTTAATAGTCATTCCATGGTCCAC
NM_013359.2       GATACATGTGGCAAGAACTTTCGTCAGAGATCAGCACTTAATAGTCATTCCATGGTCCAC
                  ************************************************************

HsCD00329644      ATAGAAGAGAAGCCATACAAATGTGAGCAATGTGGAAAAGGCTTCATTTGTAGGCGAGAT
NM_013359.2       ATAGAAGAGAAGCCATACAAATGTGAGCAATGTGGAAAAGGCTTCATTTGTAGGCGAGAT
                  ************************************************************

HsCD00329644      TTTTGTAAGCATCAGATGGTCCACACAGGAGAGAAACCATATAATTGTAAAGAATGTGGG
NM_013359.2       TTTTGTAAGCATCAGATGGTCCACACAGGAGAGAAACCATATAATTGTAAAGAATGTGGG
                  ************************************************************

HsCD00329644      AAGACCTTCAGATGGTCCTCATGTCTTTTGAACCATCAGCAAGTCCACAGTGGACAAAAA
NM_013359.2       AAGACCTTCAGATGGTCCTCATGTCTTTTGAACCATCAGCAAGTCCACAGTGGACAAAAA
                  ************************************************************

HsCD00329644      TCCTTCAAATGTGAAGAATGTGGGAAGGGATTTTATACAAATTCACGACGATCTTCCCAT
NM_013359.2       TCCTTCAAATGTGAAGAATGTGGGAAGGGATTTTATACAAATTCACGACGATCTTCCCAT
                  ************************************************************

HsCD00329644      CAGAGATCCCACAATGGAGAAAAGCCATATAACTGTGAGGAGTGTGGTAAGGACTATAAA
NM_013359.2       CAGAGATCCCACAATGGAGAAAAGCCATATAACTGTGAGGAGTGTGGTAAGGACTATAAA
                  ************************************************************

HsCD00329644      AGGAGGTTGGATCTTGAGTTTCACCAGAGGGTCCACACGGGTGAGAGACCCTATAATTGT
NM_013359.2       AGGAGGTTGGATCTTGAGTTTCACCAGAGGGTCCACACGGGTGAGAGACCCTATAATTGT
                  ************************************************************

HsCD00329644      AAGGAATGTGGCAAGAGCTTTGGCTGGGCCTCCTGTCTTTTGAAACATCAGAGACTCCAC
NM_013359.2       AAGGAATGTGGCAAGAGCTTTGGCTGGGCCTCCTGTCTTTTGAAACATCAGAGACTCCAC
                  ************************************************************

HsCD00329644      AGTGGGGAAAAACCTTTCAAATGTGAAGAGTGTGGAAAGAGATTTACTCAGAGTACACAA
NM_013359.2       AGTGGGGAAAAACCTTTCAAATGTGAAGAGTGTGGAAAGAGATTTACTCAGAGTTCACAA
                  ******************************************************:*****

HsCD00329644      CTTCATTCCCATCAGACATGCCATACTGGAGAAAAGCTATACAAATGTGAGCAGTGTGAG
NM_013359.2       CTTCATTCCCATCAGACATGCCATACTGGAGAAAAGCTATACAAATGTGAGCAGTGTGAG
                  ************************************************************

HsCD00329644      AAGGGGTACAACAGTAAATTTAATCTTGACATGCACCAGAGGGTCCACAGGGGAGAGCGA
NM_013359.2       AAGGGGTACAACAGTAAATTTAATCTTGACATGCACCAGAGGGTCCACGGGGGAGAGCGA
                  ************************************************.***********

HsCD00329644      CCCTATAATTGTAAGGAATGTGGAAAGAGCTTTGGCTGGGCTTCATGTCTTTTGAAACAT
NM_013359.2       CCCTATAATTGTAAGGAATGTGGAAAGAGCTTTGGCTGGGCTTCATGTCTTTTGAAACAT
                  ************************************************************

HsCD00329644      CAGAGACTCCACAGTGGAGAAAAGCCATTGAAATCTGGAGTGTGGGAAGAGATCTACTCA
NM_013359.2       CAGAGACTCCACAGTGGAGAAAAGCCATTGAAATCTGGAGTGTGGGAAGAGATCTACTCA
                  ************************************************************

HsCD00329644      GAATTCACAGCTTCATTTACATCAGTAAGTCTATGTGGGAGAAAAGCCATATTG
NM_013359.2       GAATTCACAGCTTCATTTACATCAGTAAGTCTATGTGGGAGAAAAGCCATATAA
                  ****************************************************:.

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q9UK13
HPRD : 10323

Clone

Cloning Information : 268093
HIP Master Clone ID : 193126
Original Clone ID : FLH268093.01L

Organism


TAX_ID : 9606
Species Specific ID: 7638

Genome

Chromosome : 19
Map Location : 19q13.2
Ensembl : ENSG00000159905

Reagents

Labome : ZNF221-antibody