DNASU Plasmid Repository • 480.965.5697 | Email

NFKBIL1 (H. sapiens ) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  NFKBIL1
Gene Name:  nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1
Sequence             
Original Clone ID: FLH268097.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329648

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1146nts         Open reading frame : 1 to 1146
Reference Sequence Alignment

ATGAGTAACCCCTCCCCCCAGGTTCCAGAGGAAGAAGCCTCCACATCTGTCTGCCGGCCC
AAGAGTTCCATGGCCTCCACTTCCCGCCGCCAACGCCGAGAACGTCGCTTTCGTCGTTAC
TTGTCTGCAGGACGGCTGGTCCGGGCCCAGGCCCTCCTCCAGCGACACCCAGGCCTCGAT
GTAGATGCTGGGCAGCCCCCACCACTGCACCGGGCCTGTGCCCGCCACGATGCCCCTGCC
CTGTGCCTGCTGCTTCGGCTCGGGGCTGACCCTGCCCACCAGGACCGCCATGGGGACACG
GCACTGCATGCTGCTGCCCGCCAGGGCCCAGATGCCTACACCGATTTCTTCCTCCCGCTG
CTAAGCCGCTGTCCCTCCGCCATGGGAATAAAGAATAAGGATGGGGAGACCCCTGGCCAA
ATTTTGGGCTGGGGACCCCCCTGGGATTCTGCTGAAGAGGAGGAAGAAGATGATGCCTCC
AAGGAGCGGGAATGGAGACAGAAGCTCCAGGGTGAGCTGGAGGACGAGTGGCAGGAAGTC
ATGGGGAGGTTTGAAGGTGATGCCTCCCATGAAACCCAGGAACCTGAGTCCTTCTCAGCC
TGGTCAGATCGCCTGGCCCGGGAACATGCCCAGAAGTGCCAGCAGCAGCAGCGAGAAGCA
GAGGGATCCTGTCGACCCCCACGTGCTGAGGGCTCCAGCCAGAGCTGGCGACAGCAGGAG
GAGGAGCAGCGGCTCTTCAGGGAGCGAGCCCGGGCCAAGGAGGAAGAGCTGCGTGAGAGC
CGAGCCAGGAGGGCGCAGGAGGCTCTAGGGGACCGAGAACCCAAGCCAACCAGGGCCGGG
CCCAGGGAAGAGCACCCCAGAGGAGCGGGGAGGGGCAGCCTCTGGCGATTTGGTGATGTG
CCCTGGCCCTGCCCTGGGGGAGGGGACCCAGAGGCCATGGCTGCAGCCCTGGTGGCCAGG
GGCCCCCCTTTGGAGGAACAGGGGGCTCTGAGGAGGTACTTGAGGGTCCAGCAGGTCCGC
TGGCACCCTGACCGCTTCCTGCAGCGATTCCGAAGCCAGATTGAGACCTGGGAGCTGGGC
CGTGTGATGGGAGCAGTGACAGCCCTTTCTCAGGCCCTGAATCGCCATGCAGAGGCCCTC
AAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1203
Start on reference sequence 58
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NFKBIL1
Symbol Nomenclature : NFKBIL1
SYNONYM : IKBL; LST1; NFKBIL
Designation : I-kappa-B-like protein|NF-kappa-B inhibitor-like protein 1|ikappaBL|inhibitor of kappa B-like protein
Full Nomenclature : nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1
GENEID : 4795
Locus Tag : DADB-70P7.2
HGNC : 7800
MIM : 601022
Vega : OTTHUMG00000031038
Target GenBank: BC105068

 

Reference Sequence Alignment

  

HsCD00329648        ATGAGTAACCCCTCCCCCCAGGTTCCAGAGGAAGAAGCCTCCACATCTGTCTGCCGGCCC
NM_005007.3         ATGAGTAACCCCTCCCCCCAGGTTCCAGAGGAAGAAGCCTCCACATCTGTCTGCCGGCCC
NM_001144962.1      ------------------------------------------------------------
NM_001144961.1      ATGAGTAACCCCTCCCCCCAGGTTCCAGAGGAAGAAGCCTCCACATCTGTCTGCCGGCCC
                                                                                

HsCD00329648        AAGAGTTCCATGGCCTCCACTTCCCGCCGCCAACGCCGAGAACGTCGCTTTCGTCGTTAC
NM_005007.3         AAGAGTTCCATGGCCTCCACTTCCCGCCGCCAACGCCGAGAACGTCGCTTTCGTCGTTAC
NM_001144962.1      ---------ATGGCCTCCACTTCCCGCCGCCAACGCCGAGAACGTCGCTTTCGTCGTTAC
NM_001144961.1      AAGAGTTCCATGGCCTCCACTTCCCGCCGCCAACGCCGAGAACGTCGCTTTCGTCGTTAC
                             ***************************************************

HsCD00329648        TTGTCTGCAGGACGGCTGGTCCGGGCCCAGGCCCTCCTCCAGCGACACCCAGGCCTCGAT
NM_005007.3         TTGTCTGCAGGACGGCTGGTCCGGGCCCAGGCCCTCCTCCAGCGACACCCAGGCCTCGAT
NM_001144962.1      TTGTCTGCAGGACGGCTGGTCCGGGCCCAGGCCCTCCTCCAGCGACACCCAGGCCTCGAT
NM_001144961.1      TTGTCTGCAGGACGGCTGGTCCGGGCCCAGGCCCTCCTCCAGCGACACCCAGGCCTCGAT
                    ************************************************************

HsCD00329648        GTAGATGCTGGGCAGCCCCCACCACTGCACCGGGCCTGTGCCCGCCACGATGCCCCTGCC
NM_005007.3         GTAGATGCTGGGCAGCCCCCACCACTGCACCGGGCCTGTGCCCGCCACGATGCCCCTGCC
NM_001144962.1      GTAGATGCTGGGCAGCCCCCACCACTGCACCGGGCCTGTGCCCGCCACGATGCCCCTGCC
NM_001144961.1      GTAGATGCTGGGCAGCCCCCACCACTGCACCGGGCCTGTGCCCGCCACGATGCCCCTGCC
                    ************************************************************

HsCD00329648        CTGTGCCTGCTGCTTCGGCTCGGGGCTGACCCTGCCCACCAGGACCGCCATGGGGACACG
NM_005007.3         CTGTGCCTGCTGCTTCGGCTCGGGGCTGACCCTGCCCACCAGGACCGCCATGGGGACACG
NM_001144962.1      CTGTGCCTGCTGCTTCGGCTCGGGGCTGACCCTGCCCACCAGGACCGCCATGGGGACACG
NM_001144961.1      CTGTGCCTGCTGCTTCGGCTCGGGGCTGACCCTGCCCACCAGGACCGCCATGGGGACACG
                    ************************************************************

HsCD00329648        GCACTGCATGCTGCTGCCCGCCAGGGCCCAGATGCCTACACCGATTTCTTCCTCCCGCTG
NM_005007.3         GCACTGCATGCTGCTGCCCGCCAGGGCCCAGATGCCTACACCGATTTCTTCCTCCCGCTG
NM_001144962.1      GCACTGCATGCTGCTGCCCGCCAGGGCCCAGATGCCTACACCGATTTCTTCCTCCCGCTG
NM_001144961.1      GCACTGCATGCTGCTGCCCGCCAGGGCCCAGATGCCTACACCGATTTCTTCCTCCCGCTG
                    ************************************************************

HsCD00329648        CTAAGCCGCTGTCCCTCCGCCATGGGAATAAAGAATAAGGATGGGGAGACCCCTGGCCAA
NM_005007.3         CTAAGCCGCTGTCCCTCCGCCATGGGAATAAAGAATAAGGATGGGGAGACCCCTGGCCAA
NM_001144962.1      CTAAGCCGCTGTCCCTCCGCCATGGGAATAAAGAATAAGGATGGGGAGACCCCTGGCCAA
NM_001144961.1      CTAAGCCGCTGTCCCTCCGCCATGGGAATAAAGAATAAGGATGGGGAGACCCCTGGCCAA
                    ************************************************************

HsCD00329648        ATTTTGGGCTGGGGACCCCCCTGGGATTCTGCTGAAGAGGAGGAAGAAGATGATGCCTCC
NM_005007.3         ATTTTGGGCTGGGGACCCCCCTGGGATTCTGCTGAAGAGGAGGAAGAAGATGATGCCTCC
NM_001144962.1      ATTTTGGGCTGGGGACCCCCCTGGGATTCTGCTGAAGAGGAGGAAGAAGATGATGCCTCC
NM_001144961.1      ATTTTGGGCTGGGGACCCCCCTGGGATTCTGCTGAAGAGGAGGAAGAAGATGATGCCTCC
                    ************************************************************

HsCD00329648        AAGGAGCGGGAATGGAGACAGAAGCTCCAGGGTGAGCTGGAGGACGAGTGGCAGGAAGTC
NM_005007.3         AAGGAGCGGGAATGGAGACAGAAGCTCCAGGGTGAGCTGGAGGACGAGTGGCAGGAAGTC
NM_001144962.1      AAGGAGCGGGAATGGAGACAGAAGCTCCAGGGTGAGCTGGAGGACGAGTGGCAGGAAGTC
NM_001144961.1      AAGGAGCGGGAATGGAGACAGAAGCTCCAGG-----------------------------
                    *******************************                             

HsCD00329648        ATGGGGAGGTTTGAAGGTGATGCCTCCCATGAAACCCAGGAACCTGAGTCCTTCTCAGCC
NM_005007.3         ATGGGGAGGTTTGAAGGTGATGCCTCCCATGAAACCCAGGAACCTGAGTCCTTCTCAGCC
NM_001144962.1      ATGGGGAGGTTTGAAGGTGATGCCTCCCATGAAACCCAGGAACCTGAGTCCTTCTCAGCC
NM_001144961.1      ----------------GTGATGCCTCCCATGAAACCCAGGAACCTGAGTCCTTCTCAGCC
                                    ********************************************

HsCD00329648        TGGTCAGATCGCCTGGCCCGGGAACATGCCCAGAAGTGCCAGCAGCAGCAGCGAGAAGCA
NM_005007.3         TGGTCAGATCGCCTGGCCCGGGAACATGCCCAGAAGTGCCAGCAGCAGCAGCGAGAAGCA
NM_001144962.1      TGGTCAGATCGCCTGGCCCGGGAACATGCCCAGAAGTGCCAGCAGCAGCAGCGAGAAGCA
NM_001144961.1      TGGTCAGATCGCCTGGCCCGGGAACATGCCCAGAAGTGCCAGCAGCAGCAGCGAGAAGCA
                    ************************************************************

HsCD00329648        GAGGGATCCTGTCGACCCCCACGTGCTGAGGGCTCCAGCCAGAGCTGGCGACAGCAGGAG
NM_005007.3         GAGGGATCCCGTCGACCCCCACGTGCTGAGGGCTCCAGCCAGAGCTGGCGACAGCAGGAG
NM_001144962.1      GAGGGATCCCGTCGACCCCCACGTGCTGAGGGCTCCAGCCAGAGCTGGCGACAGCAGGAG
NM_001144961.1      GAGGGATCCCGTCGACCCCCACGTGCTGAGGGCTCCAGCCAGAGCTGGCGACAGCAGGAG
                    ********* **************************************************

HsCD00329648        GAGGAGCAGCGGCTCTTCAGGGAGCGAGCCCGGGCCAAGGAGGAAGAGCTGCGTGAGAGC
NM_005007.3         GAGGAGCAGCGGCTCTTCAGGGAGCGAGCCCGGGCCAAGGAGGAAGAGCTGCGTGAGAGC
NM_001144962.1      GAGGAGCAGCGGCTCTTCAGGGAGCGAGCCCGGGCCAAGGAGGAAGAGCTGCGTGAGAGC
NM_001144961.1      GAGGAGCAGCGGCTCTTCAGGGAGCGAGCCCGGGCCAAGGAGGAAGAGCTGCGTGAGAGC
                    ************************************************************

HsCD00329648        CGAGCCAGGAGGGCGCAGGAGGCTCTAGGGGACCGAGAACCCAAGCCAACCAGGGCCGGG
NM_005007.3         CGAGCCAGGAGGGCGCAGGAGGCTCTAGGGGACCGAGAACCCAAGCCAACCAGGGCCGGG
NM_001144962.1      CGAGCCAGGAGGGCGCAGGAGGCTCTAGGGGACCGAGAACCCAAGCCAACCAGGGCCGGG
NM_001144961.1      CGAGCCAGGAGGGCGCAGGAGGCTCTAGGGGACCGAGAACCCAAGCCAACCAGGGCCGGG
                    ************************************************************

HsCD00329648        CCCAGGGAAGAGCACCCCAGAGGAGCGGGGAGGGGCAGCCTCTGGCGATTTGGTGATGTG
NM_005007.3         CCCAGGGAAGAGCACCCCAGAGGAGCGGGGAGGGGCAGCCTCTGGCGATTTGGTGATGTG
NM_001144962.1      CCCAGGGAAGAGCACCCCAGAGGAGCGGGGAGGGGCAGCCTCTGGCGATTTGGTGATGTG
NM_001144961.1      CCCAGGGAAGAGCACCCCAGAGGAGCGGGGAGGGGCAGCCTCTGGCGATTTGGTGATGTG
                    ************************************************************

HsCD00329648        CCCTGGCCCTGCCCTGGGGGAGGGGACCCAGAGGCCATGGCTGCAGCCCTGGTGGCCAGG
NM_005007.3         CCCTGGCCCTGCCCTGGGGGAGGGGACCCAGAGGCCATGGCTGCAGCCCTGGTGGCCAGG
NM_001144962.1      CCCTGGCCCTGCCCTGGGGGAGGGGACCCAGAGGCCATGGCTGCAGCCCTGGTGGCCAGG
NM_001144961.1      CCCTGGCCCTGCCCTGGGGGAGGGGACCCAGAGGCCATGGCTGCAGCCCTGGTGGCCAGG
                    ************************************************************

HsCD00329648        GGCCCCCCTTTGGAGGAACAGGGGGCTCTGAGGAGGTACTTGAGGGTCCAGCAGGTCCGC
NM_005007.3         GGCCCCCCTTTGGAGGAACAGGGGGCTCTGAGGAGGTACTTGAGGGTCCAGCAGGTCCGC
NM_001144962.1      GGCCCCCCTTTGGAGGAACAGGGGGCTCTGAGGAGGTACTTGAGGGTCCAGCAGGTCCGC
NM_001144961.1      GGCCCCCCTTTGGAGGAACAGGGGGCTCTGAGGAGGTACTTGAGGGTCCAGCAGGTCCGC
                    ************************************************************

HsCD00329648        TGGCACCCTGACCGCTTCCTGCAGCGATTCCGAAGCCAGATTGAGACCTGGGAGCTGGGC
NM_005007.3         TGGCACCCTGACCGCTTCCTGCAGCGATTCCGAAGCCAGATTGAGACCTGGGAGCTGGGC
NM_001144962.1      TGGCACCCTGACCGCTTCCTGCAGCGATTCCGAAGCCAGATTGAGACCTGGGAGCTGGGC
NM_001144961.1      TGGCACCCTGACCGCTTCCTGCAGCGATTCCGAAGCCAGATTGAGACCTGGGAGCTGGGC
                    ************************************************************

HsCD00329648        CGTGTGATGGGAGCAGTGACAGCCCTTTCTCAGGCCCTGAATCGCCATGCAGAGGCCCTC
NM_005007.3         CGTGTGATGGGAGCAGTGACAGCCCTTTCTCAGGCCCTGAATCGCCATGCAGAGGCCCTC
NM_001144962.1      CGTGTGATGGGAGCAGTGACAGCCCTTTCTCAGGCCCTGAATCGCCATGCAGAGGCCCTC
NM_001144961.1      CGTGTGATGGGAGCAGTGACAGCCCTTTCTCAGGCCCTGAATCGCCATGCAGAGGCCCTC
                    ************************************************************

HsCD00329648        AAGTTG
NM_005007.3         AAGTGA
NM_001144962.1      AAGTGA
NM_001144961.1      AAGTGA
                    **** .

Pathways

Panther : B cell activation

Protein

UniProt : Q9UBC1
UniProt : A8K778
UniProt : Q5STV4
UniProt : Q5ST96
UniProt : Q5STV6
HPRD : 07043

Clone

Cloning Information : 268097
HIP Master Clone ID : 193131
Original Clone ID : FLH268097.01L

Organism


TAX_ID : 9606
Species Specific ID: 4795

Genome

Chromosome : 6
Map Location : 6p21.3
Ensembl : ENSG00000204498

Reagents

Labome : NFKBIL1-antibody