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DAZAP1 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  DAZAP1
Gene Name:  DAZ associated protein 1
Sequence             
Original Clone ID: FLH268102.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329653

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1224nts         Open reading frame : 1 to 1224
Reference Sequence Alignment

ATGAACAACTCGGGCGCCGACGAGATCGGGAAGCTCTTCGTGGGCGGTCTTGACTGGAGC
ACGACCCAAGAGACTCTGCGCAGCTACTTTTCCCAATATGGAGAAGTCGTAGATTGTGTT
ATCATGAAAGATAAAACCACCAACCAGTCTCGAGGCTTTGGGTTTGTCAAATTTAAAGAC
CCAAACTGTGTGGGGACGGTGCTGGCCAGCAGACCGCACACGCTAGATGGCCGAAACATC
GACCCCAAGCCATGCACACCCCGGGGGATGCAGCCGGAGAGAACACGGCCGAAGGAAGGA
TGGCAGAAAGGACCCAGGAGCGATAACAGTAAATCAAATAAGATATTTGTCGGTGGAATT
CCTCACAATTGTGGTGAGACAGAGCTCAGGGAATACTTCAAGAAGTTCGGAGTGGTCACG
GAGGTAGTCATGATCTATGACGCCGAGAAGCAGAGGCCCCGAGGTTTTGGATTTATTACT
TTCGAGGACGAACAATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGGGC
AAAAAAGTGGAAGTTAAACGAGCTGAGCCTCGGGACAGCAAGAGCCAAGCGCCGGGACAG
CCAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAACGCTGCCAATGGCTGGGCAGGC
CAGCCCCCGCCCACGTGGCAGCAAGGATATGGCCCGCAAGGAATGTGGGTGCCGGCAGGA
CAGGCGATTGGTGGCTATGGACCGCCCCCTGCAGGAAGAGGAGCCCCCCCGCCACCCCCA
CCGTTCACCTCCTACATCGTGTCCACCCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTC
CCTCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCACCG
CCAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACCAGCCACTCCCGGGGCAGCACCT
CTGGCTTTCCCACCGCCTCCGTCTCAGGCTGCCCCGGACATGAGCAAGCCCCCGACAGCT
CAGCCAGACTTCCCCTATGGTCAGTATGCAGGTTACGGGCAGGACTTGAGTGGCTTCGGA
CAGGGCTTCTCAGACCCCAGCCAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAGGG
TCGGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCACAACGTGCAA
GGGTTCCACCCCTACCGACGCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1297
Start on reference sequence 74
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : DAZAP1
Symbol Nomenclature : DAZAP1
Designation : DAZ-associated protein 1|deleted in azoospermia-associated protein 1
Full Nomenclature : DAZ associated protein 1
GENEID : 26528
HGNC : 2683
MIM : 607430
Vega : OTTHUMG00000180058
Target GenBank: BC012062

 

Reference Sequence Alignment

  

HsCD00329653        ATGAACAACTCGGGCGCCGACGAGATCGGGAAGCTCTTCGTGGGCGGTCTTGACTGGAGC
XM_005259531.1      ATGAACAACTCGGGCGCCGACGAGATCGGGAAGCTCTTCGTGGGCGGTCTTGACTGGAGC
XM_005259532.1      ATGAACAACTCGGGCGCCGACGAGATCGGGAAGCTCTTCGTGGGCGGTCTTGACTGGAGC
XM_005259530.1      ATGAACAACTCGGGCGCCGACGAGATCGGGAAGCTCTTCGTGGGCGGTCTTGACTGGAGC
NM_018959.3         ATGAACAACTCGGGCGCCGACGAGATCGGGAAGCTCTTCGTGGGCGGTCTTGACTGGAGC
                    ************************************************************

HsCD00329653        ACGACCCAAGAGACTCTGCGCAGCTACTTTTCCCAATATGGAGAAGTCGTAGATTGTGTT
XM_005259531.1      ACGACCCAAGAGACTCTGCGCAGCTACTTTTCCCAATATGGAGAAGTCGTAGATTGTGTT
XM_005259532.1      ACGACCCAAGAGACTCTGCGCAGCTACTTTTCCCAATATGGAGAAGTCGTAGATTGTGTT
XM_005259530.1      ACGACCCAAGAGACTCTGCGCAGCTACTTTTCCCAATATGGAGAAGTCGTAGATTGTGTT
NM_018959.3         ACGACCCAAGAGACTCTGCGCAGCTACTTTTCCCAATATGGAGAAGTCGTAGATTGTGTT
                    ************************************************************

HsCD00329653        ATCATGAAAGATAAAACCACCAACCAGTCTCGAGGCTTTGGGTTTGTCAAATTTAAAGAC
XM_005259531.1      ATCATGAAAGATAAAACCACCAACCAGTCTCGAGGCTTTGGGTTTGTCAAATTTAAAGAC
XM_005259532.1      ATCATGAAAGATAAAACCACCAACCAGTCTCGAGGCTTTGGGTTTGTCAAATTTAAAGAC
XM_005259530.1      ATCATGAAAGATAAAACCACCAACCAGTCTCGAGGCTTTGGGTTTGTCAAATTTAAAGAC
NM_018959.3         ATCATGAAAGATAAAACCACCAACCAGTCTCGAGGCTTTGGGTTTGTCAAATTTAAAGAC
                    ************************************************************

HsCD00329653        CCAAACTGTGTGGGGACGGTGCTGGCCAGCAGACCGCACACGCTAGATGGCCGAAACATC
XM_005259531.1      CCAAACTGTGTGGGGACGGTGCTGGCCAGCAGACCGCACACGCTAGATGGCCGAAACATC
XM_005259532.1      CCAAACTGTGTGGGGACGGTGCTGGCCAGCAGACCGCACACGCTAGATGGCCGAAACATC
XM_005259530.1      CCAAACTGTGTGGGGACGGTGCTGGCCAGCAGACCGCACACGCTAGATGGCCGAAACATC
NM_018959.3         CCAAACTGTGTGGGGACGGTGCTGGCCAGCAGACCGCACACGCTAGATGGCCGAAACATC
                    ************************************************************

HsCD00329653        GACCCCAAGCCATGCACACCCCGGGGGATGCAGCCGGAGAGAACACGGCCGAAGGAAGGA
XM_005259531.1      GACCCCAAGCCATGCACACCCCGGGGGATGCAGCCGGAGAGAACACGGCCGAAGGAAGGA
XM_005259532.1      GACCCCAAGCCATGCACACCCCGGGGGATGCAGCCGGAGAGAACACGGCCGAAGGAAGGA
XM_005259530.1      GACCCCAAGCCATGCACACCCCGGGGGATGCAGCCGGAGAGAACACGGCCGAAGGAAGGA
NM_018959.3         GACCCCAAGCCATGCACACCCCGGGGGATGCAGCCGGAGAGAACACGGCCGAAGGAAGGA
                    ************************************************************

HsCD00329653        TGGCAGAAAGGACCCAGGAGCGATAACAGTAAATCAAATAAGATATTTGTCGGTGGAATT
XM_005259531.1      TG---GAAAGGACCCAGGAGCGATAACAGTAAATCAAATAAGATATTTGTCGGTGGAATT
XM_005259532.1      TG---GAAAGGACCCAGGAGCGATAACAGTAAATCAAATAAGATATTTGTCGGTGGAATT
XM_005259530.1      TGGCAGAAAGGACCCAGGAGCGATAACAGTAAATCAAATAAGATATTTGTCGGTGGAATT
NM_018959.3         TGGCAGAAAGGACCCAGGAGCGATAACAGTAAATCAAATAAGATATTTGTCGGTGGAATT
                    **   *******************************************************

HsCD00329653        CCTCACAATTGTGGTGAGACAGAGCTCAGGGAATACTTCAAGAAGTTCGGAGTGGTCACG
XM_005259531.1      CCTCACAATTGTGGTGAGACAGAGCTCAGGGAATACTTCAAGAAGTTCGGAGTGGTCACG
XM_005259532.1      CCTCACAATTGTGGTGAGACAGAGCTCAGGGAATACTTCAAGAAGTTCGGAGTGGTCACG
XM_005259530.1      CCTCACAATTGTGGTGAGACAGAGCTCAGGGAATACTTCAAGAAGTTCGGAGTGGTCACG
NM_018959.3         CCTCACAATTGTGGTGAGACAGAGCTCAGGGAATACTTCAAGAAGTTCGGAGTGGTCACG
                    ************************************************************

HsCD00329653        GAGGTAGTCATGATCTATGACGCCGAGAAGCAGAGGCCCCGAGGTTTTGGATTTATTACT
XM_005259531.1      GAGGTAGTCATGATCTATGACGCCGAGAAGCAGAGGCCCCGAGGTTTTGGATTTATTACT
XM_005259532.1      GAGGTAGTCATGATCTATGACGCCGAGAAGCAGAGGCCCCGAGGTTTTGGATTTATTACT
XM_005259530.1      GAGGTAGTCATGATCTATGACGCCGAGAAGCAGAGGCCCCGAGGTTTTGGATTTATTACT
NM_018959.3         GAGGTAGTCATGATCTATGACGCCGAGAAGCAGAGGCCCCGAGGTTTTGGATTTATTACT
                    ************************************************************

HsCD00329653        TTCGAGGACGAACAATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGGGC
XM_005259531.1      TTCGAGGACGAACAATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGGGC
XM_005259532.1      TTCGAGGACGAACAATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGGGC
XM_005259530.1      TTCGAGGACGAACAATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGGGC
NM_018959.3         TTCGAGGACGAACAATCAGTGGACCAGGCTGTCAACATGCATTTTCACGACATCATGGGC
                    ************************************************************

HsCD00329653        AAAAAAGTGGAAGTTAAACGAGCTGAGCCTCGGGACAGCAAGAGCCAAGCGCCGGGACAG
XM_005259531.1      AAAAAAGTGGAAGTTAAACGAGCTGAGCCTCGGGACAGCAAGAGCCAAGCGCCGGGACAG
XM_005259532.1      AAAAAAGTGGAAGTTAAACGAGCTGAGCCTCGGGACAGCAAGAGCCAAGCGCCGGGACAG
XM_005259530.1      AAAAAAGTGGAAGTTAAACGAGCTGAGCCTCGGGACAGCAAGAGCCAAGCGCCGGGACAG
NM_018959.3         AAAAAAGTGGAAGTTAAACGAGCTGAGCCTCGGGACAGCAAGAGCCAAGCGCCGGGACAG
                    ************************************************************

HsCD00329653        CCAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAACGCTGCCAATGGCTGGGCAGGC
XM_005259531.1      CCAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAACGCTGCCAATGGCTGGGCAGGC
XM_005259532.1      CCAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAACGCTGCCAATGGCTGGGCAGGC
XM_005259530.1      CCAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAACGCTGCCAATGGCTGGGCAGGC
NM_018959.3         CCAGGTGCCAGCCAGTGGGGGAGCCGGGTTGTGCCCAACGCTGCCAATGGCTGGGCAGGC
                    ************************************************************

HsCD00329653        CAGCCCCCGCCCACGTGGCAGCAAGGATATGGCCCGCAAGGAATGTGGGTGCCGGCAGGA
XM_005259531.1      CAGCCCCCGCCCACGTGGCAGCAAGGATATGGCCCGCAAGGAATGTGGGTGCCGGCAGGA
XM_005259532.1      CAGCCCCCGCCCACGTGGCAGCAAGGATATGGCCCGCAAGGAATGTGGGTGCCGGCAGGA
XM_005259530.1      CAGCCCCCGCCCACGTGGCAGCAAGGATATGGCCCGCAAGGAATGTGGGTGCCGGCAGGA
NM_018959.3         CAGCCCCCGCCCACGTGGCAGCAAGGATATGGCCCGCAAGGAATGTGGGTGCCGGCAGGA
                    ************************************************************

HsCD00329653        CAGGCGATTGGTGGCTATGGACCGCCCCCTGCAGGAAGAGGAGCCCCCCCGCCACCCCCA
XM_005259531.1      CAGGCGATTGGTGGCTATGGACCGCCCCCTGCAGGAAGAGGAGCCCCCCCGCCACCCCCA
XM_005259532.1      CAGGCGATTGGTGGCTATGGACCGCCCCCTGCAGGAAGAGGAGCCCCCCCGCCACCCCCA
XM_005259530.1      CAGGCGATTGGTGGCTATGGACCGCCCCCTGCAGGAAGAGGAGCCCCCCCGCCACCCCCA
NM_018959.3         CAGGCGATTGGTGGCTATGGACCGCCCCCTGCAGGAAGAGGAGCCCCCCCGCCACCCCCA
                    ************************************************************

HsCD00329653        CCGTTCACCTCCTACATCGTGTCCACCCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTC
XM_005259531.1      CCGTTCACCTCCTACATCGTGTCCACCCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTC
XM_005259532.1      CCGTTCACCTCCTACATCGTGTCCACCCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTC
XM_005259530.1      CCGTTCACCTCCTACATCGTGTCCACCCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTC
NM_018959.3         CCGTTCACCTCCTACATCGTGTCCACCCCTCCTGGAGGCTTTCCCCCTCCCCAGGGCTTC
                    ************************************************************

HsCD00329653        CCTCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCACCG
XM_005259531.1      CCTCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCACCG
XM_005259532.1      CCTCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCACCG
XM_005259530.1      CCTCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCACCG
NM_018959.3         CCTCAGGGCTACGGTGCCCCGCCACAGTTCAGTTTTGGCTACGGGCCTCCACCTCCACCG
                    ************************************************************

HsCD00329653        CCAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACCAGCCACTCCCGGGGCAGCACCT
XM_005259531.1      CCAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACCAGCCACTCCCGGGGCAGCACCT
XM_005259532.1      CCAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACCAGCCACTCCCGGGGCAGCACCT
XM_005259530.1      CCAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACCAGCCACTCCCGGGGCAGCACCT
NM_018959.3         CCAGATCAGTTTGCCCCTCCGGGGGTTCCTCCTCCACCAGCCACTCCCGGGGCAGCACCT
                    ************************************************************

HsCD00329653        CTGGCTTTCCCACCGCCTCCGTCTCAGGCTGCCCCGGACATGAGCAAGCCCCCGACAGCT
XM_005259531.1      CTGGCTTTCCCACCGCCTCCGTCTCAGGCTGCCCCGGACATGAGCAAGCCCCCGACAGCT
XM_005259532.1      CTGGCTTTCCCACCGCCTCCGTCTCAGGCTGCCCCGGACATGAGCAAGCCCCCGACAGCT
XM_005259530.1      CTGGCTTTCCCACCGCCTCCGTCTCAGGCTGCCCCGGACATGAGCAAGCCCCCGACAGCT
NM_018959.3         CTGGCTTTCCCACCGCCTCCGTCTCAGGCTGCCCCGGACATGAGCAAGCCCCCGACAGCT
                    ************************************************************

HsCD00329653        CAGCCAGACTTCCCCTATGGTCAGTATGCAGGTTACGGGCAGGACTTGAGTGGCTTCGGA
XM_005259531.1      CAGCCAGACTTCCCCTATGGTCAGTATGCAGGTTACGGGCAGGACTTGAGTGGCTTCGGA
XM_005259532.1      CAGCCAGACTTCCCCTATGGTCAGTAT---GGTTACGGGCAGGACTTGAGTGGCTTCGGA
XM_005259530.1      CAGCCAGACTTCCCCTATGGTCAGTAT---GGTTACGGGCAGGACTTGAGTGGCTTCGGA
NM_018959.3         CAGCCAGACTTCCCCTATGGTCAGTATGCAGGTTACGGGCAGGACTTGAGTGGCTTCGGA
                    ***************************   ******************************

HsCD00329653        CAGGGCTTCTCAGACCCCAGCCAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAGGG
XM_005259531.1      CAGGGCTTCTCAGACCCCAGCCAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAGGG
XM_005259532.1      CAGGGCTTCTCAGACCCCAGCCAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAGGG
XM_005259530.1      CAGGGCTTCTCAGACCCCAGCCAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAGGG
NM_018959.3         CAGGGCTTCTCAGACCCCAGCCAGCAGCCTCCTTCCTACGGGGGTCCCTCCGTGCCAGGG
                    ************************************************************

HsCD00329653        TCGGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCACAACGTGCAA
XM_005259531.1      TCGGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCACAACGTGCAA
XM_005259532.1      TCGGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCACAACGTGCAA
XM_005259530.1      TCGGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCACAACGTGCAA
NM_018959.3         TCGGGGGGCCCCCCCGCCGGCGGCAGCGGCTTTGGACGAGGGCAGAACCACAACGTGCAA
                    ************************************************************

HsCD00329653        GGGTTCCACCCCTACCGACGCTTG
XM_005259531.1      GGGTTCCACCCCTACCGACGCTAG
XM_005259532.1      GGGTTCCACCCCTACCGACGCTAG
XM_005259530.1      GGGTTCCACCCCTACCGACGCTAG
NM_018959.3         GGGTTCCACCCCTACCGACGCTAG
                    **********************:*

Protein

SMART domain : RRM : RNA recognition motif
UniProt : Q96EP5
HPRD : 08462

Clone

Cloning Information : 268102
HIP Master Clone ID : 193136
Original Clone ID : FLH268102.01L

Organism


TAX_ID : 9606
Species Specific ID: 26528

Genome

Chromosome : 19
Map Location : 19p13.3
Ensembl : ENSG00000071626

Reagents

Labome : DAZAP1-antibody