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ZNF556 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF556
Gene Name:  zinc finger protein 556
Sequence             
Original Clone ID: FLH268128.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329673

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1368nts         Open reading frame : 1 to 1368
Reference Sequence Alignment

ATGGACACAGTGGTCTTTGAAGACGTGGTTGTGGATTTCACGCTGGAGGAGTGGGCCTTG
CTGAATCCTGCTCAGAGAAAACTCTACAGAGATGTCATGCTGGAGACCTTCAAGCACCTG
GCCTCAGTAGATAATGAGGCTCAGCTTAAAGCCAGTGGGTCTATTTCTCAGCAGGATACT
TCTGGAGAAAAATTATCCCTCAAACAGAAAATAGAAAAGTTCACAAGAAAGAATATATGG
GCCTCCCTTTTAGGAAAAAATTGGGAAGAACATAGCGTTAAAGACAAGCACAACACCAAG
GAGAGACATTTGAGAAATCCAAGGGTGGAGAGACCATGTAAAAGCAGTAAAGGTAATAAA
CGTGGAAGAACCTTCAGAAAGACTCGAAATTGTAATCGTCATCTGCGCAAGAATTGTTGT
ACTAGTGTAAGACGGTACGAATGCAGTCAGTGTGGAAAACTCTTCACCCATTCCTCATCC
CTGATAAGGCACAAAAGAGCTCACTCTGGACAAAAATTATATAAATGTAAGGAATGTGGG
AAAGCCTTCAGTCGCCCTTCCTACCTACAGACGCATGAGAAAACTCACAGTGGAGAGAAA
CCCTATGCCTGTCAATCTTGCGGGAAGACATTTCTTCGTTCCCACTCTCTCACTGAACAT
GTAAGGACTCACACTGGAGAGAAACCCTACGAATGTGGGCAGTGTGGGAAAGGCTTCAGT
TGTCCCAAATCCTTTCGCGCACATGTGATGATGCACGCCGGAGGGAGACCGTATGAGTGC
AAGCACTGTGGGAAAGCCTTCAGGTGTCAGAAATCCTTTCGAGTCCATATGATCATGCAC
GCCGGAGGGAGACCGTATGAGTGCAAGCAGTGTGGGAAAGCCTACTGCTGGGCAACATCC
TTTCAACGACACGTGAGAATTCACAACGGGGAGAAACCCTATAAGTGTGGAAAATGCGGG
AAAGCATTCGGTTGGCCCTCATCCTTACACAAACACGCGAGAACGCATGCTAAAAAGAAA
CCTGTGAGTGGGGGCAGCGTGGGAAAGTCTTCCGCGAGGCCTCGCCCCTCCACAGATGTC
AAATCACAAACTAGAGAGAAAGTCTATAAATGTGAAACGTGTGGGAAAACGTATGGTTGG
TCCTCATCTTTACACAAACATGAGAGAAAGCACACTGGGGAGAAACCTGTAAATGCAGCC
AGTGTGGGAAAACCTTCAGGCGGGCTTTGCTCTTCCAAAAATGTAAGAACGCAGATTGGA
CAGAAGCCCAGTAAATGCGAAAAATGTGGGAAAGCTTTCAGTTGTCCCAAAGCCTTTCAA
GGTCATGTGAGAAGTCACACAGGAAAGAAATCCTGTACATCTAAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1444
Start on reference sequence 77
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF556
Symbol Nomenclature : ZNF556
Full Nomenclature : zinc finger protein 556
GENEID : 80032
HGNC : 25669
Vega : OTTHUMG00000180501
Target GenBank: BC009374

 

Reference Sequence Alignment

  

HsCD00329673        ATGGACACAGTGGTCTTTGAAGACGTGGTTGTGGATTTCACGCTGGAGGAGTGGGCCTTG
NM_024967.1         ATGGACACAGTGGTCTTTGAAGACGTGGTTGTGGATTTCACGCTGGAGGAGTGGGCCTTG
XM_005259647.1      ATGGACACAGTGGTCTTTGAAGACGTGGTTGTGGATTTCACGCTGGAGGAGTGGGCCTTG
                    ************************************************************

HsCD00329673        CTGAATCCTGCTCAGAGAAAACTCTACAGAGATGTCATGCTGGAGACCTTCAAGCACCTG
NM_024967.1         CTGAATCCTGCTCAGAGAAAACTCTACAGAGATGTCATGCTGGAGACCTTCAAGCACCTG
XM_005259647.1      CTGAATCCTGCTCAGAGAAAACTCTACAGAGATGTCATGCTGGAGACCTTCAAGCACCTG
                    ************************************************************

HsCD00329673        GCCTCAGTAGATAATGAGGCTCAGCTTAAAGCCAGTGGGTCTATTTCTCAGCAGGATACT
NM_024967.1         GCCTCAGTAGATAATGAGGCTCAGCTTAAAGCCAGTGGGTCTATTTCTCAGCAGGATACT
XM_005259647.1      GCCTCAGTAGATAATGAGGCTCAGCTTAAAGCCAGTGGGTCTATTTCTCAGCAGGATACT
                    ************************************************************

HsCD00329673        TCTGGAGAAAAATTATCCCTCAAACAGAAAATAGAAAAGTTCACAAGAAAGAATATATGG
NM_024967.1         TCTGGAGAAAAATTATCCCTCAAACAGAAAATAGAAAAGTTCACAAGAAAGAATATATGG
XM_005259647.1      TCTGGAGAAAAATTATCCCTCAAACAGAAAATAGAAAAGTTCACAAGAAAGAATATATGG
                    ************************************************************

HsCD00329673        GCCTCCCTTTTAGGAAAAAATTGGGAAGAACATAGCGTTAAAGACAAGCACAACACCAAG
NM_024967.1         GCCTCCCTTTTAGGAAAAAATTGGGAAGAACATAGCGTTAAAGACAAGCACAACACCAAG
XM_005259647.1      GCCTCCCTTTTAGGAAAAAATTGGGAAGAACATAGCGTTAAAGACAAGCACAACACCAAG
                    ************************************************************

HsCD00329673        GAGAGACATTTG---AGAAATCCAAGGGTGGAGAGACCATGTAAAAGCAGTAAAGGTAAT
NM_024967.1         GAGAGACATTTGAGCAGAAATCCAAGGGTGGAGAGACCGTGTAAAAGCAGTAAAGGTAAT
XM_005259647.1      GAGAGACATTT---GAGAAATCCAAGGGTGGAGAGACCATGTAAAAGCAGTAAAGGTAAT
                    ***********    ***********************.*********************

HsCD00329673        AAACGTGGAAGAACCTTCAGAAAGACTCGAAATTGTAATCGTCATCTGCGCAAGAATTGT
NM_024967.1         AAACGTGGAAGAACCTTCAGAAAGACTCGAAATTGTAATCGTCATCTGCGCAAGAATTGT
XM_005259647.1      AAACGTGGAAGAACCTTCAGAAAGACTCGAAATTGTAATCGTCATCTGCGCAAGAATTGT
                    ************************************************************

HsCD00329673        TGTACTAGTGTAAGACGGTACGAATGCAGTCAGTGTGGAAAACTCTTCACCCATTCCTCA
NM_024967.1         TGTACTAGTGTAAGACGGTACGAATGCAGTCAGTGTGGAAAACTCTTCACCCATTCCTCA
XM_005259647.1      TGTACTAGTGTAAGACGGTACGAATGCAGTCAGTGTGGAAAACTCTTCACCCATTCCTCA
                    ************************************************************

HsCD00329673        TCCCTGATAAGGCACAAAAGAGCTCACTCTGGACAAAAATTATATAAATGTAAGGAATGT
NM_024967.1         TCCCTGATAAGGCACAAAAGAGCTCACTCTGGACAAAAATTATATAAATGTAAGGAATGT
XM_005259647.1      TCCCTGATAAGGCACAAAAGAGCTCACTCTGGACAAAAATTATATAAATGTAAGGAATGT
                    ************************************************************

HsCD00329673        GGGAAAGCCTTCAGTCGCCCTTCCTACCTACAGACGCATGAGAAAACTCACAGTGGAGAG
NM_024967.1         GGGAAAGCCTTCAGTCGCCCTTCCTACCTACAGACGCATGAGAAAACTCACAGTGGAGAG
XM_005259647.1      GGGAAAGCCTTCAGTCGCCCTTCCTACCTACAGACGCATGAGAAAACTCACAGTGGAGAG
                    ************************************************************

HsCD00329673        AAACCCTATGCCTGTCAATCTTGCGGGAAGACATTTCTTCGTTCCCACTCTCTCACTGAA
NM_024967.1         AAACCCTATGCCTGTCAATCTTGCGGGAAGACATTTCTTCGTTCCCACTCTCTCACTGAA
XM_005259647.1      AAACCCTATGCCTGTCAATCTTGCGGGAAGACATTTCTTCGTTCCCACTCTCTCACTGAA
                    ************************************************************

HsCD00329673        CATGTAAGGACTCACACTGGAGAGAAACCCTACGAATGTGGGCAGTGTGGGAAAGGCTTC
NM_024967.1         CATGTAAGGACTCACACTGGAGAGAAACCCTACGAATGTGGGCAGTGTGGGAAAGGCTTC
XM_005259647.1      CATGTAAGGACTCACACTGGAGAGAAACCCTACGAATGTGGGCAGTGTGGGAAAGGCTTC
                    ************************************************************

HsCD00329673        AGTTGTCCCAAATCCTTTCGCGCACATGTGATGATGCACGCCGGAGGGAGACCGTATGAG
NM_024967.1         AGTTGTCCCAAATCCTTTCGCGCACATGTGATGATGCACGCCGGAGGGAGACCGTATGAG
XM_005259647.1      AGTTGTCCCAAATCCTTTCGCGCACATGTGATGATGCACGCCGGAGGGAGACCGTATGAG
                    ************************************************************

HsCD00329673        TGCAAGCACTGTGGGAAAGCCTTCAGGTGTCAGAAATCCTTTCGAGTCCATATGATCATG
NM_024967.1         TGCAAGCACTGTGGGAAAGCCTTCAGGTGTCAGAAATCCTTTCGAGTCCATATGATCATG
XM_005259647.1      TGCAAGCACTGTGGGAAAGCCTTCAGGTGTCAGAAATCCTTTCGAGTCCATATGATCATG
                    ************************************************************

HsCD00329673        CACGCCGGAGGGAGACCGTATGAGTGCAAGCAGTGTGGGAAAGCCTACTGCTGGGCAACA
NM_024967.1         CACGCCGGAGGGAGACCGTATGAGTGCAAGCAGTGTGGGAAAGCCTACTGCTGGGCAACA
XM_005259647.1      CACGCCGGAGGGAGACCGTATGAGTGCAAGCAGTGTGGGAAAGCCTACTGCTGGGCAACA
                    ************************************************************

HsCD00329673        TCCTTTCAACGACACGTGAGAATTCACAACGGGGAGAAACCCTATAAGTGTGGAAAATGC
NM_024967.1         TCCTTTCAACGACACGTGAGAATTCACAACGGGGAGAAACCCTATAAGTGTGGAAAATGC
XM_005259647.1      TCCTTTCAACGACACGTGAGAATTCACAACGGGGAGAAACCCTATAAGTGTGGAAAATGC
                    ************************************************************

HsCD00329673        GGGAAAGCATTCGGTTGGCCCTCATCCTTACACAAACACGCGAGAACGCATGCTAAAAAG
NM_024967.1         GGGAAAGCATTCGGTTGGCCCTCATCCTTACACAAACACGCGAGAACGCATGCTAAAAAG
XM_005259647.1      GGGAAAGCATTCGGTTGGCCCTCATCCTTACACAAACACGCGAGAACGCATGCTAAAAAG
                    ************************************************************

HsCD00329673        AAACCTGTGAGTGGGGGCAGCGTGGGAAAGTCTTCCGCGAGGCCTCGCCCCTCCACAGAT
NM_024967.1         AAACCTGTGAGTGGGGGCAGCGTGGGAAAGTCTTCCGCGAGGCCTCGCCCCTCCACAGAT
XM_005259647.1      AAACCTGTGAGTGGGGGCAGCGTGGGAAAGTCTTCCGCGAGGCCTCGCCCCTCCACAGAT
                    ************************************************************

HsCD00329673        GTCAAATCACAAACTAGAGAGAAAGTCTATAAATGTGAAACGTGTGGGAAAACGTATGGT
NM_024967.1         GTCAAATCACAAACTAGAGAGAAAGTCTATAAATGTGAAACGTGTGGGAAAACGTATGGT
XM_005259647.1      GTCAAATCACAAACTAGAGAGAAAGTCTATAAATGTGAAACGTGTGGGAAAACGTATGGT
                    ************************************************************

HsCD00329673        TGGTCCTCATCTTTACACAAACATGAGAGAAAGCACACTGGGGAGAAACCTGTAAATGCA
NM_024967.1         TGGTCCTCATCTTTACACAAACATGAGAGAAAGCACACTGGGGAGAAACCTGTAAATGCA
XM_005259647.1      TGGTCCTCATCTTTACACAAACATGAGAGAAAGCACACTGGGGAGAAACCTGTAAATGCA
                    ************************************************************

HsCD00329673        GCCAGTGTGGGAAAACCTTCAGGCGGGCTTTGCTCTTCCAAAAATGTAAGAACGCAGATT
NM_024967.1         GCCAGTGTGGGAAAACCTTCAGGCGGGCTTTGCTCTTCCAAAAATGTAAGAACGCAGATT
XM_005259647.1      GCCAGTGTGGGAAAACCTTCAGGCGGGCTTTGCTCTTCCAAAAATGTAAGAACGCAGATT
                    ************************************************************

HsCD00329673        GGACAGAAGCCCAGTAAATGCGAAAAATGTGGGAAAGCTTTCAGTTGTCCCAAAGCCTTT
NM_024967.1         GGACAGAAGCCCAGTAAATGCGAAAAATGTGGGAAAGCTTTCAGTTGTCCCAAAGCCTTT
XM_005259647.1      GGACAGAAGCCCAGTAAATGCGAAAAATGTGGGAAAGCTTTCAGTTGTCCCAAAGCCTTT
                    ************************************************************

HsCD00329673        CAAGGTCATGTGAGAAGTCACACAGGAAAGAAATCCTGTACATCTAAGTTG
NM_024967.1         CAAGGTCATGTGAGAAGTCACACAGGAAAGAAATCCTGTACATCTAAGTAA
XM_005259647.1      CAAGGTCATGTGAGAAGTCACACAGGAAAGAAATCCTGTACATCTAAGTAA
                    *************************************************:.

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q9HAH1
HPRD : 15839

Clone

Cloning Information : 268128
HIP Master Clone ID : 193166
Original Clone ID : FLH268128.01L

Organism


TAX_ID : 9606
Species Specific ID: 80032

Genome

Chromosome : 19
Map Location : 19p13.3
Ensembl : ENSG00000172000

Reagents

Labome : ZNF556-antibody