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ZA20D2 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZFAND5
Gene Name:  zinc finger, AN1-type domain 5
Sequence             
Original Clone ID: FLH268142.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329685

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 642nts         Open reading frame : 1 to 642
Reference Sequence Alignment

ATGGCTCAGGAGACTAACCAGACCCCGGGGCCCATGCTGTGTAGCACAGGATGTGGCTTT
TATGGAAATCCTAGGACAAATGGAATGTGTTCAGTTTGCTACAAAGAACATCTTCAGAGG
CAGCAAAATAGTGGCAGAATGAGCCCAATGGGGACAGCTAGTGGTTCCAACAGTCCTACC
TCAGATTCTGCATCTGTACAGAGAGCAGACACTAGCTTAAACAACTGTGAAGGTGCTGCT
GGCAGCACATCTGAAAAATCAAGAAATGTGCCTGTGGCTGCCTTGCCTGTAACTCAGCAA
ATGACAGAAATGAGCATTTCAAGAGAGGACAAAATAACTACCCCGAAAACAGAGGTGTCA
GAGCCAGTTGTCACTCAGCCCAGTCCATCAGTTTCTCAGCCCAGTACTTCTCAGAGTGAA
GAAAAAGCTCCTGAATTGCCCAAACCAAAGAAAAACAGATGTTTCATGTGCAGAAAGAAA
GTTGGTCTTACAGGGTTTGACTGCCGATGTGGAAATTTGTTTTGTGGACTTCACCGTTAC
TCTGACAAGCACAACTGTCCGTATGATTACAAAGCAGAAGCTGCAGCAAAAATCAGAAAA
GAGAATCCAGTTGTTGTGGCTGAAAAAATTCAGAGAATATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 704
Start on reference sequence 63
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZFAND5
Symbol Nomenclature : ZFAND5
SYNONYM : ZA20D2; ZFAND5A; ZNF216
Designation : AN1-type zinc finger protein 5|zinc finger A20 domain-containing protein 2|zinc finger protein 216|zinc finger, A20 domain containing 2
Full Nomenclature : zinc finger, AN1-type domain 5
GENEID : 7763
Locus Tag : RP11-63P12.8
HGNC : 13008
MIM : 604761
Target GenBank: BC073131

 

Reference Sequence Alignment

  

HsCD00329685        ATGGCTCAGGAGACTAACCAGACCCCGGGGCCCATGCTGTGTAGCACAGGATGTGGCTTT
NM_001278243.1      ATGGCTCAGGAGACTAACCAGACCCCGGGGCCCATGCTGTGTAGCACAGGATGTGGCTTT
NM_006007.3         ATGGCTCAGGAGACTAACCAGACCCCGGGGCCCATGCTGTGTAGCACAGGATGTGGCTTT
NM_001278244.1      ATGGCTCAGGAGACTAACCAGACCCCGGGGCCCATGCTGTGTAGCACAGGATGTGGCTTT
NM_001102421.2      ATGGCTCAGGAGACTAACCAGACCCCGGGGCCCATGCTGTGTAGCACAGGATGTGGCTTT
NM_001102420.2      ATGGCTCAGGAGACTAACCAGACCCCGGGGCCCATGCTGTGTAGCACAGGATGTGGCTTT
NM_001278245.1      ATGGCTCAGGAGACTAACCAGACCCCGGGGCCCATGCTGTGTAGCACAGGATGTGGCTTT
                    ************************************************************

HsCD00329685        TATGGAAATCCTAGGACAAATGGAATGTGTTCAGTTTGCTACAAAGAACATCTTCAGAGG
NM_001278243.1      TATGGAAATCCTAGGACAAATGGAATGTGTTCAGTTTGCTACAAAGAACATCTTCAGAGG
NM_006007.3         TATGGAAATCCTAGGACAAATGGAATGTGTTCAGTTTGCTACAAAGAACATCTTCAGAGG
NM_001278244.1      TATGGAAATCCTAGGACAAATGGAATGTGTTCAGTTTGCTACAAAGAACATCTTCAGAGG
NM_001102421.2      TATGGAAATCCTAGGACAAATGGAATGTGTTCAGTTTGCTACAAAGAACATCTTCAGAGG
NM_001102420.2      TATGGAAATCCTAGGACAAATGGAATGTGTTCAGTTTGCTACAAAGAACATCTTCAGAGG
NM_001278245.1      TATGGAAATCCTAGGACAAATGGAATGTGTTCAGTTTGCTACAAAGAACATCTTCAGAGG
                    ************************************************************

HsCD00329685        CAGCAAAATAGTGGCAGAATGAGCCCAATGGGGACAGCTAGTGGTTCCAACAGTCCTACC
NM_001278243.1      CAGCAAAATAGTGGCAGAATGAGCCCAATGGGGACAGCTAGTGGTTCCAACAGTCCTACC
NM_006007.3         CAGCAAAATAGTGGCAGAATGAGCCCAATGGGGACAGCTAGTGGTTCCAACAGTCCTACC
NM_001278244.1      CAGCAAAATAGTGGCAGAATGAGCCCAATGGGGACAGCTAGTGGTTCCAACAGTCCTACC
NM_001102421.2      CAGCAAAATAGTGGCAGAATGAGCCCAATGGGGACAGCTAGTGGTTCCAACAGTCCTACC
NM_001102420.2      CAGCAAAATAGTGGCAGAATGAGCCCAATGGGGACAGCTAGTGGTTCCAACAGTCCTACC
NM_001278245.1      CAGCAAAATAGTGGCAGAATGAGCCCAATGGGGACAGCTAGTGGTTCCAACAGTCCTACC
                    ************************************************************

HsCD00329685        TCAGATTCTGCATCTGTACAGAGAGCAGACACTAGCTTAAACAACTGTGAAGGTGCTGCT
NM_001278243.1      TCAGATTCTGCATCTGTACAGAGAGCAGACACTAGCTTAAACAACTGTGAAGGTGCTGCT
NM_006007.3         TCAGATTCTGCATCTGTACAGAGAGCAGACACTAGCTTAAACAACTGTGAAGGTGCTGCT
NM_001278244.1      TCAGATTCTGCATCTGTACAGAGAGCAGACACTAGCTTAAACAACTGTGAAGGTGCTGCT
NM_001102421.2      TCAGATTCTGCATCTGTACAGAGAGCAGACACTAGCTTAAACAACTGTGAAGGTGCTGCT
NM_001102420.2      TCAGATTCTGCATCTGTACAGAGAGCAGACACTAGCTTAAACAACTGTGAAGGTGCTGCT
NM_001278245.1      TCAGATTCTGCATCTGTACAGAGAGCAGACACTAGCTTAAACAACTGTGAAGGTGCTGCT
                    ************************************************************

HsCD00329685        GGCAGCACATCTGAAAAATCAAGAAATGTGCCTGTGGCTGCCTTGCCTGTAACTCAGCAA
NM_001278243.1      GGCAGCACATCTGAAAAATCAAGAAATGTGCCTGTGGCTGCCTTGCCTGTAACTCAGCAA
NM_006007.3         GGCAGCACATCTGAAAAATCAAGAAATGTGCCTGTGGCTGCCTTGCCTGTAACTCAGCAA
NM_001278244.1      GGCAGCACATCTGAAAAATCAAGAAATGTGCCTGTGGCTGCCTTGCCTGTAACTCAGCAA
NM_001102421.2      GGCAGCACATCTGAAAAATCAAGAAATGTGCCTGTGGCTGCCTTGCCTGTAACTCAGCAA
NM_001102420.2      GGCAGCACATCTGAAAAATCAAGAAATGTGCCTGTGGCTGCCTTGCCTGTAACTCAGCAA
NM_001278245.1      GGCAGCACATCTGAAAAATCAAGAAATGTGCCTGTGGCTGCCTTGCCTGTAACTCAGCAA
                    ************************************************************

HsCD00329685        ATGACAGAAATGAGCATTTCAAGAGAGGACAAAATAACTACCCCGAAAACAGAGGTGTCA
NM_001278243.1      ATGACAGAAATGAGCATTTCAAGAGAGGACAAAATAACTACCCCGAAAACAGAGGTGTCA
NM_006007.3         ATGACAGAAATGAGCATTTCAAGAGAGGACAAAATAACTACCCCGAAAACAGAGGTGTCA
NM_001278244.1      ATGACAGAAATGAGCATTTCAAGAGAGGACAAAATAACTACCCCGAAAACAGAGGTGTCA
NM_001102421.2      ATGACAGAAATGAGCATTTCAAGAGAGGACAAAATAACTACCCCGAAAACAGAGGTGTCA
NM_001102420.2      ATGACAGAAATGAGCATTTCAAGAGAGGACAAAATAACTACCCCGAAAACAGAGGTGTCA
NM_001278245.1      ATGACAGAAATGAGCATTTCAAGAGAGGACAAAATAACTACCCCGAAAACAGAGGTGTCA
                    ************************************************************

HsCD00329685        GAGCCAGTTGTCACTCAGCCCAGTCCATCAGTTTCTCAGCCCAGTACTTCTCAGAGTGAA
NM_001278243.1      GAGCCAGTTGTCACTCAGCCCAGTCCATCAGTTTCTCAGCCCAGTACTTCTCAGAGTGAA
NM_006007.3         GAGCCAGTTGTCACTCAGCCCAGTCCATCAGTTTCTCAGCCCAGTACTTCTCAGAGTGAA
NM_001278244.1      GAGCCAGTTGTCACTCAGCCCAGTCCATCAGTTTCTCAGCCCAGTACTTCTCAGAGTGAA
NM_001102421.2      GAGCCAGTTGTCACTCAGCCCAGTCCATCAGTTTCTCAGCCCAGTACTTCTCAGAGTGAA
NM_001102420.2      GAGCCAGTTGTCACTCAGCCCAGTCCATCAGTTTCTCAGCCCAGTACTTCTCAGAGTGAA
NM_001278245.1      GAGCCAGTTGTCACTCAGCCCAGTCCATCAGTTTCTCAGCCCAGTACTTCTCAGAGTGAA
                    ************************************************************

HsCD00329685        GAAAAAGCTCCTGAATTGCCCAAACCAAAGAAAAACAGATGTTTCATGTGCAGAAAGAAA
NM_001278243.1      GAAAAAGCTCCTGAATTGCCCAAACCAAAGAAAAACAGATGTTTCATGTGCAGAAAGAAA
NM_006007.3         GAAAAAGCTCCTGAATTGCCCAAACCAAAGAAAAACAGATGTTTCATGTGCAGAAAGAAA
NM_001278244.1      GAAAAAGCTCCTGAATTGCCCAAACCAAAGAAAAACAGATGTTTCATGTGCAGAAAGAAA
NM_001102421.2      GAAAAAGCTCCTGAATTGCCCAAACCAAAGAAAAACAGATGTTTCATGTGCAGAAAGAAA
NM_001102420.2      GAAAAAGCTCCTGAATTGCCCAAACCAAAGAAAAACAGATGTTTCATGTGCAGAAAGAAA
NM_001278245.1      GAAAAAGCTCCTGAATTGCCCAAACCAAAGAAAAACAGATGTTTCATGTGCAGAAAGAAA
                    ************************************************************

HsCD00329685        GTTGGTCTTACAGGGTTTGACTGCCGATGTGGAAATTTGTTTTGTGGACTTCACCGTTAC
NM_001278243.1      GTTGGTCTTACAGGGTTTGACTGCCGATGTGGAAATTTGTTTTGTGGACTTCACCGTTAC
NM_006007.3         GTTGGTCTTACAGGGTTTGACTGCCGATGTGGAAATTTGTTTTGTGGACTTCACCGTTAC
NM_001278244.1      GTTGGTCTTACAGGGTTTGACTGCCGATGTGGAAATTTGTTTTGTGGACTTCACCGTTAC
NM_001102421.2      GTTGGTCTTACAGGGTTTGACTGCCGATGTGGAAATTTGTTTTGTGGACTTCACCGTTAC
NM_001102420.2      GTTGGTCTTACAGGGTTTGACTGCCGATGTGGAAATTTGTTTTGTGGACTTCACCGTTAC
NM_001278245.1      GTTGGTCTTACAGGGTTTGACTGCCGATGTGGAAATTTGTTTTGTGGACTTCACCGTTAC
                    ************************************************************

HsCD00329685        TCTGACAAGCACAACTGTCCGTATGATTACAAAGCAGAAGCTGCAGCAAAAATCAGAAAA
NM_001278243.1      TCTGACAAGCACAACTGTCCGTATGATTACAAAGCAGAAGCTGCAGCAAAAATCAGAAAA
NM_006007.3         TCTGACAAGCACAACTGTCCGTATGATTACAAAGCAGAAGCTGCAGCAAAAATCAGAAAA
NM_001278244.1      TCTGACAAGCACAACTGTCCGTATGATTACAAAGCAGAAGCTGCAGCAAAAATCAGAAAA
NM_001102421.2      TCTGACAAGCACAACTGTCCGTATGATTACAAAGCAGAAGCTGCAGCAAAAATCAGAAAA
NM_001102420.2      TCTGACAAGCACAACTGTCCGTATGATTACAAAGCAGAAGCTGCAGCAAAAATCAGAAAA
NM_001278245.1      TCTGACAAGCACAACTGTCCGTATGATTACAAAGCAGAAGCTGCAGCAAAAATCAGAAAA
                    ************************************************************

HsCD00329685        GAGAATCCAGTTGTTGTGGCTGAAAAAATTCAGAGAATATTG
NM_001278243.1      GAGAATCCAGTTGTTGTGGCTGAAAAAATTCAGAGAATATAA
NM_006007.3         GAGAATCCAGTTGTTGTGGCTGAAAAAATTCAGAGAATATAA
NM_001278244.1      GAGAATCCAGTTGTTGTGGCTGAAAAAATTCAGAGAATATAA
NM_001102421.2      GAGAATCCAGTTGTTGTGGCTGAAAAAATTCAGAGAATATAA
NM_001102420.2      GAGAATCCAGTTGTTGTGGCTGAAAAAATTCAGAGAATATAA
NM_001278245.1      GAGAATCCAGTTGTTGTGGCTGAAAAAATTCAGAGAATATAA
                    ****************************************:.

Pathways

NCI : FoxO family signaling

Protein

SMART domain : ZnF_A20 : A20-like zinc fingers
SMART domain : ZnF_AN1 : AN1-like Zinc finger
UniProt : O76080
HPRD : 05302

Clone

Cloning Information : 268142
HIP Master Clone ID : 193184
Original Clone ID : FLH268142.01L

Organism


TAX_ID : 9606
Species Specific ID: 7763

Genome

Chromosome : 9
Map Location : 9q13-q21
Ensembl : ENSG00000107372

Reagents

Labome : ZFAND5-antibody