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ZNF75 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF75D
Gene Name:  zinc finger protein 75D
Sequence             
Original Clone ID: FLH268143.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329686

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1533nts         Open reading frame : 1 to 1533
Reference Sequence Alignment

ATGGCGATGAGAGAGCTGAACGCGGATTCATGCTCAAGCCCCCAGATGGGGGCTATGTGG
GAGACTAGTGGGTCTGTGAAAGAGAACTCCAGTCAGAGTAAGAAATACAGCACAAAAATA
GAGAATCTTGGTCCTGAGAGCGCTTGCAGGCACTTCTGGAGCTTCCGTTATCATGAAGCA
ACCGGACCGCTTGAGACTATCAGCCAACTTCAGAAATTGTGCCATCAGTGGCTGAGGCCA
GAGATCCACTCAAAAGAGCAGATCTTGGAAATGCTGGTGTTAGAGCAGTTCCTGAGCATT
CTGCCCAAGGAGACCCAGAACTGGGTGCAGAAGCATCATCCACAGAATGTCAAACAGGCT
CTGGTCCTGGTGGAATTCTTGCAGAGGGAGCCTGATGGAACAAAGAATGAGGTCACAGCC
CATGAGCTGGGAAAGGAGGCAGTGCTCTTGGGAGGAACAGCAGTGGCCCCAGGCTTCAAG
TGGAAGCCAGCAGAGCCCCAACCAATGGGTGTGTTCCAGAAAGAATATTGGAATACATAC
CGGGTACTACAAGAACAGCTGGGCTGGAACACTCACAAAGAAACCCAGCCTGTATATGAA
AGAGCTGTGCATGATCAACAGATGTTAGCCCTTTCTGAGCAGAAAAGAATCAAACACTGG
AAGATGGCATCTAAACTCATCCTGCCTGAGTCCCTGAGTTTGTTGACATTTGAAGATGTG
GCTGTGTATTTTTCTGAGGAAGAGTGGCAATTATTGAATCCTCTTGAGAAGACTCTCTAC
AATGATGTAATGCAGGATATCTATGAGACTGTCATCTCTCTAGGGTTAAAGCTAAAAAAT
GACACTGGAAATGATCATCCTATATCTGTTTCTACATCAGAAATACAAACATCAGGATGC
GAAGTATCAAAAAAGACCAGAATGAAAATTGCCCAGAAAACAATGGGCAGGGAAAATCCT
GGTGATACACACAGTGTACAGAAATGGCATCGAGCTTTTCCAAGGAAGAAAAGAAAGAAA
CCTGCAACTTGTAAACAAGAGCTTCCAAAACTTATGGATCTTCATGGGAAAGGCCCCACA
GGGGAGAAACCTTTTAAGTGTCAGGAATGTGGGAAAAGCTTCAGAGTTAGCTCTGATCTT
ATTAAACACCACAGAATTCACACTGGAGAGAAACCCTATAAATGTCAACAATGTGACAGG
AGGTTTAGATGGAGTTCAGATCTTAATAAGCACTTCATGACCCATCAAGGAATAAAACCA
TATAGATGCTCATGGTGTGGGAAAAGCTTTAGTCATAACACAAATCTACACACACACCAA
AGAATTCACACAGGAGAGAAGCCCTTTAAATGTGATGAATGTGGAAAAAGATTCATTCAG
AACTCCCACCTTATTAAACACCAGAGAACTCACACAGGTGAGCAGCCTTATACATGTAGC
TTATGCAAGAGAAACTTTAGTAGGCGATCGAGCCTTCTTAGACACCAGAAACTCCACAGA
AGAAGGGAAGCATGTCTAGTGTCTCCAAACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1762
Start on reference sequence 230
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF75D
Symbol Nomenclature : ZNF75D
SYNONYM : D8C6; ZKSCAN24; ZNF75; ZNF82; ZSCAN28
Designation : zinc finger protein 75|zinc finger protein 82
Full Nomenclature : zinc finger protein 75D
GENEID : 7626
Locus Tag : RP13-210D15.7
HGNC : 13145
MIM : 314997
Vega : OTTHUMG00000022482
Target GenBank: BC109100

 

Reference Sequence Alignment

  

HsCD00329686        ATGGCGATGAGAGAGCTGAACGCGGATTCATGCTCAAGCCCCCAGATGGGGGCTATGTGG
XM_005262469.1      ATGGCGATGAGAGAGCTGAACGCGGATTCATGCTCAAGCCCCCAGATGGGGGCTATGTGG
                    ************************************************************

HsCD00329686        GAGACTAGTGGGTCTGTGAAAGAGAACTCCAGTCAGAGTAAGAAATACAGCACAAAAATA
XM_005262469.1      GAGACTAGTGGGTCTGTGAAAGAGAACTCCAGTCAGAGTAAGAAATACAGCACAAAAATA
                    ************************************************************

HsCD00329686        GAGAATCTTGGTCCTGAGAGCGCTTGCAGGCACTTCTGGAGCTTCCGTTATCATGAAGCA
XM_005262469.1      GAGAATCTTGGTCCTGAGAGCGCTTGCAGGCACTTCTGGAGCTTCCGTTATCATGAAGCA
                    ************************************************************

HsCD00329686        ACCGGACCGCTTGAGACTATCAGCCAACTTCAGAAATTGTGCCATCAGTGGCTGAGGCCA
XM_005262469.1      ACCGGACCGCTTGAGACTATCAGCCAACTTCAGAAATTGTGCCATCAGTGGCTGAGGCCA
                    ************************************************************

HsCD00329686        GAGATCCACTCAAAAGAGCAGATCTTGGAAATGCTGGTGTTAGAGCAGTTCCTGAGCATT
XM_005262469.1      GAGATCCACTCAAAAGAGCAGATCTTGGAAATGCTGGTGTTAGAGCAGTTCCTGAGCATT
                    ************************************************************

HsCD00329686        CTGCCCAAGGAGACCCAGAACTGGGTGCAGAAGCATCATCCACAGAATGTCAAACAGGCT
XM_005262469.1      CTGCCCAAGGAGACCCAGAACTGGGTGCAGAAGCATCATCCACAGAATGTCAAACAGGCT
                    ************************************************************

HsCD00329686        CTGGTCCTGGTGGAATTCTTGCAGAGGGAGCCTGATGGAACAAAGAATGAGGTCACAGCC
XM_005262469.1      CTGGTCCTGGTGGAATTCTTGCAGAGGGAGCCTGATGGAACAAAGAATGAGGTCACAGCC
                    ************************************************************

HsCD00329686        CATGAGCTGGGAAAGGAGGCAGTGCTCTTGGGAGGAACAGCAGTGGCCCCAGGCTTCAAG
XM_005262469.1      CATGAGCTGGGAAAGGAGGCAGTGCTCTTGGGAGGAACAGCAGTGGCCCCAGGCTTCAAG
                    ************************************************************

HsCD00329686        TGGAAGCCAGCAGAGCCCCAACCAATGGGTGTGTTCCAGAAAGAATATTGGAATACATAC
XM_005262469.1      TGGAAGCCAGCAGAGCCCCAACCAATGGGTGTGTTCCAGAAAGAATATTGGAATACATAC
                    ************************************************************

HsCD00329686        CGGGTACTACAAGAACAGCTGGGCTGGAACACTCACAAAGAAACCCAGCCTGTATATGAA
XM_005262469.1      CGGGTACTACAAGAACAGCTGGGCTGGAACACTCACAAAGAAACCCAGCCTGTATATGAA
                    ************************************************************

HsCD00329686        AGAGCTGTGCATGATCAACAGATGTTAGCCCTTTCTGAGCAGAAAAGAATCAAACACTGG
XM_005262469.1      AGAGCTGTGCATGATCAACAGATGTTAGCCCTTTCTGAGCAGAAAAGAATCAAACACTGG
                    ************************************************************

HsCD00329686        AAGATGGCATCTAAACTCATCCTGCCTGAGTCCCTGAGTTTGTTGACATTTGAAGATGTG
XM_005262469.1      AAGATGGCATCTAAACTCATCCTGCCTGAGTCCCTGAGTTTGTTGACATTTGAAGATGTG
                    ************************************************************

HsCD00329686        GCTGTGTATTTTTCTGAGGAAGAGTGGCAATTATTGAATCCTCTTGAGAAGACTCTCTAC
XM_005262469.1      GCTGTGTATTTTTCTGAGGAAGAGTGGCAATTATTGAATCCTCTTGAGAAGACTCTCTAC
                    ************************************************************

HsCD00329686        AATGATGTAATGCAGGATATCTATGAGACTGTCATCTCTCTAGGGTTAAAGCTAAAAAAT
XM_005262469.1      AATGATGTAATGCAGGATATCTATGAGACTGTCATCTCTCTAGGGTTAAAGCTAAAAAAT
                    ************************************************************

HsCD00329686        GACACTGGAAATGATCATCCTATATCTGTTTCTACATCAGAAATACAAACATCAGGATGC
XM_005262469.1      GACACTGGAAATGATCATCCTATATCTGTTTCTACATCAGAAATACAAACATCAGGATGC
                    ************************************************************

HsCD00329686        GAAGTATCAAAAAAGACCAGAATGAAAATTGCCCAGAAAACAATGGGCAGGGAAAATCCT
XM_005262469.1      GAAGTATCAAAAAAGACCAGAATGAAAATTGCCCAGAAAACAATGGGCAGGGAAAATCCT
                    ************************************************************

HsCD00329686        GGTGATACACACAGTGTACAGAAATGGCATCGAGCTTTTCCAAGGAAGAAAAGAAAGAAA
XM_005262469.1      GGTGATACACACAGTGTACAGAAATGGCATCGAGCTTTTCCAAGGAAGAAAAGAAAGAAA
                    ************************************************************

HsCD00329686        CCTGCAACTTGTAAACAAGAGCTTCCAAAACTTATGGATCTTCATGGGAAAGGCCCCACA
XM_005262469.1      CCTGCAACTTGTAAACAAGAGCTTCCAAAACTTATGGATCTTCATGGGAAAGGCCCCACA
                    ************************************************************

HsCD00329686        GGGGAGAAACCTTTTAAGTGTCAGGAATGTGGGAAAAGCTTCAGAGTTAGCTCTGATCTT
XM_005262469.1      GGGGAGAAACCTTTTAAGTGTCAGGAATGTGGGAAAAGCTTCAGAGTTAGCTCTGATCTT
                    ************************************************************

HsCD00329686        ATTAAACACCACAGAATTCACACTGGAGAGAAACCCTATAAATGTCAACAATGTGACAGG
XM_005262469.1      ATTAAACACCACAGAATTCACACTGGAGAGAAACCCTATAAATGTCAACAATGTGACAGG
                    ************************************************************

HsCD00329686        AGGTTTAGATGGAGTTCAGATCTTAATAAGCACTTCATGACCCATCAAGGAATAAAACCA
XM_005262469.1      AGGTTTAGATGGAGTTCAGATCTTAATAAGCACTTCATGACCCATCAAGGAATAAAACCA
                    ************************************************************

HsCD00329686        TATAGATGCTCATGGTGTGGGAAAAGCTTTAGTCATAACACAAATCTACACACACACCAA
XM_005262469.1      TATAGATGCTCATGGTGTGGGAAAAGCTTTAGTCATAACACAAATCTACACACACACCAA
                    ************************************************************

HsCD00329686        AGAATTCACACAGGAGAGAAGCCCTTTAAATGTGATGAATGTGGAAAAAGATTCATTCAG
XM_005262469.1      AGAATTCACACAGGAGAGAAGCCCTTTAAATGTGATGAATGTGGAAAAAGATTCATTCAG
                    ************************************************************

HsCD00329686        AACTCCCACCTTATTAAACACCAGAGAACTCACACAGGTGAGCAGCCTTATACATGTAGC
XM_005262469.1      AACTCCCACCTTATTAAACACCAGAGAACTCACACAGGTGAGCAGCCTTATACGTGTAGC
                    *****************************************************.******

HsCD00329686        TTATGCAAGAGAAACTTTAGTAGGCGATCGAGCCTTCTTAGACACCAGAAACTCCACAGA
XM_005262469.1      TTATGCAAGAGAAACTTTAGTAGGCGATCGAGCCTTCTTAGACACCAGAAACTCCACAGA
                    ************************************************************

HsCD00329686        AGAAGGGAAGCATGTCTAGTGTCTCCAAACTTG
XM_005262469.1      AGAAGGGAAGCATGTCTAGTGTCTCCAAACTGA
                    ******************************* .

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : SCAN : leucine rich region
SMART domain : ZnF_C2H2 : zinc finger
UniProt : P51815
UniProt : B3KRI7
UniProt : A6NK62
HPRD : 02448

Clone

Cloning Information : 268143
HIP Master Clone ID : 193185
Original Clone ID : FLH268143.01L

Organism


TAX_ID : 9606
Species Specific ID: 7626

Genome

Chromosome : X
Map Location : Xq26.3
Ensembl : ENSG00000186376

Reagents

Labome : ZNF75D-antibody