DNASU Plasmid Repository • 480.965.5697 | Email

TRIM52 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  TRIM52
Gene Name:  tripartite motif containing 52
Sequence             
Original Clone ID: FLH268160.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329703

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 894nts         Open reading frame : 1 to 894
Reference Sequence Alignment

ATGGCTGGTTATGCCACTACTCCCAGCCCCATGCAGACCCTTCAGGAGGAAGCGGTGTGT
GCCATCTGCTTGGATTACTTCAAGGACCCCGTGTCCATCAGCTGTGGGCACAACTTCTGC
CGAGGGTGTGTGACCCAGCTGTGGAGTAAGGAGGACGAGGAGGACCAGAACGAGGAGGAA
GATGAATGGGAGGAGGAGGAGGACGAGGAAGCGGTGGGGGCCATGGATGGATGGGACGGC
TCCATTCGAGAGGTGTTGTATCGGGGGAATGCTGACGAAGAGTTGTTCCAAGACCAAGAT
GACGATGAACTCTGGCTCGGTGACAGTGGTATAACTAATTGGGACAACGTAGACTATATG
TGGGACGAGGAGGAAGAAGAAGAAGAGGAAGATCAGGACTATTACCTAGGAGGCTTGAGA
CCTGACCTGAGAATTGATGTCTACCGAGAAGAAGAAATACTGGAAGCATACGATGAGGAC
GAAGATGAAGAGCTGTATCCTGACATCCACCCGCCTCCTTCCTTGCCCCTTCCAGGGCAG
TTCACCTGCCCCCAGTGCCGAAAGAGCTTTACACGTCGCAGCTTTCGTCCCAACTTGCAG
CTGGCCAACATGGTCCAGATAATTCGCCAGATGTGCCCCACTCCTTATCGGGGAAACCGG
AGTAATGATCAGGGCATGTGCTTTAAACACCAGGAAGCCCTGAAACTCTTCTGTGAGGTG
GACAAAGAGGCCATCTGTGTGGTGTGCCGAGAATCCAGGAGCCACAAACAGCACAGCGTG
CTGCCTTTGGAGGAGGTGGTGCAGGAGTACCAGGAAATAAAGTTGGAAACAACTCTGGTG
GGAATACTTCAGATAGAGCAAGAAAGCATTCACAGCAAGGCCTATAATCAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1208
Start on reference sequence 315
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TRIM52
Symbol Nomenclature : TRIM52
SYNONYM : RNF102
Designation : RING finger protein 102|tripartite motif-containing 52|tripartite motif-containing protein 52
Full Nomenclature : tripartite motif containing 52
GENEID : 84851
HGNC : 19024
Target GenBank: BC007372

 

Reference Sequence Alignment

  

HsCD00329703        ATGGCTGGTTATGCCACTACTCCCAGCCCCATGCAGACCCTTCAGGAGGAAGCGGTGTGT
XM_005266000.1      ATGGCTGGTTATGCCACTACTCCCAGCCCCATGCAGACCCTTCAGGAGGAAGCGGTGTGT
NM_032765.2         ATGGCTGGTTATGCCACTACTCCCAGCCCCATGCAGACCCTTCAGGAGGAAGCGGTGTGT
                    ************************************************************

HsCD00329703        GCCATCTGCTTGGATTACTTCAAGGACCCCGTGTCCATCAGCTGTGGGCACAACTTCTGC
XM_005266000.1      GCCATCTGCTTGGATTACTTCAAGGACCCCGTGTCCATCAGCTGTGGGCACAACTTCTGC
NM_032765.2         GCCATCTGCTTGGATTACTTCAAGGACCCCGTGTCCATCAGCTGTGGGCACAACTTCTGC
                    ************************************************************

HsCD00329703        CGAGGGTGTGTGACCCAGCTGTGGAGTAAGGAGGACGAGGAGGACCAGAACGAGGAGGAA
XM_005266000.1      CGAGGGTGTGTGACCCAGCTGTGGAGTAAGGAGGACGAGGAGGACCAGAACGAGGAGGAA
NM_032765.2         CGAGGGTGTGTGACCCAGCTGTGGAGTAAGGAGGACGAGGAGGACCAGAACGAGGAGGAA
                    ************************************************************

HsCD00329703        GATGAATGGGAGGAGGAGGAGGACGAGGAAGCGGTGGGGGCCATGGATGGATGGGACGGC
XM_005266000.1      GATGAATGGGAGGAGGAGGAGGACGAGGAAGCGGTGGGGGCCATGGATGGATGGGACGGC
NM_032765.2         GATGAATGGGAGGAGGAGGAGGACGAGGAAGCGGTGGGGGCCATGGATGGATGGGACGGC
                    ************************************************************

HsCD00329703        TCCATTCGAGAGGTGTTGTATCGGGGGAATGCTGACGAAGAGTTGTTCCAAGACCAAGAT
XM_005266000.1      TCCATTCGAGAGGTGTTGTATCGGGGGAATGCTGACGAAGAGTTGTTCCAAGACCAAGAT
NM_032765.2         TCCATTCGAGAGGTGTTGTATCGGGGGAATGCTGACGAAGAGTTGTTCCAAGACCAAGAT
                    ************************************************************

HsCD00329703        GACGATGAACTCTGGCTCGGTGACAGTGGTATAACTAATTGGGACAACGTAGACTATATG
XM_005266000.1      GACGATGAACTCTGGCTCGGTGACAGTGGTATAACTAATTGGGACAACGTAGACTATATG
NM_032765.2         GACGATGAACTCTGGCTCGGTGACAGTGGTATAACTAATTGGGACAACGTAGACTATATG
                    ************************************************************

HsCD00329703        TGGGACGAGGAGGAAGAAGAAGAAGAGGAAGATCAGGACTATTACCTAGGAGGCTTGAGA
XM_005266000.1      TGGGACGAGGAGGAAGAAGAAGAAGAGGAAGATCAGGACTATTACCTAGGAGGCTTGAGA
NM_032765.2         TGGGACGAGGAGGAAGAAGAAGAAGAGGAAGATCAGGACTATTACCTAGGAGGCTTGAGA
                    ************************************************************

HsCD00329703        CCTGACCTGAGAATTGATGTCTACCGAGAAGAAGAAATACTGGAAGCATACGATGAGGAC
XM_005266000.1      CCTGACCTGAGAATTGATGTCTACCGAGAAGAAGAAATACTGGAAGCATACGATGAGGAC
NM_032765.2         CCTGACCTGAGAATTGATGTCTACCGAGAAGAAGAAATACTGGAAGCATACGATGAGGAC
                    ************************************************************

HsCD00329703        GAAGATGAAGAGCTGTATCCTGACATCCACCCGCCTCCTTCCTTGCCCCTTCCAGGGCAG
XM_005266000.1      GAAGATGAAGAGCTGTATCCTGACATCCACCCGCCTCCTTCCTTGCCCCTTCCAGGGCAG
NM_032765.2         GAAGATGAAGAGCTGTATCCTGACATCCACCCGCCTCCTTCCTTGCCCCTTCCAGGGCAG
                    ************************************************************

HsCD00329703        TTCACCTGCCCCCAGTGCCGAAAGAGCTTTACACGTCGCAGCTTTCGTCCCAACTTGCAG
XM_005266000.1      TTCACCTGCCCCCAGTGCCGAAAGAGCTTTACACGTCGCAGCTTTCGTCCCAACTTGCAG
NM_032765.2         TTCACCTGCCCCCAGTGCCGAAAGAGCTTTACACGTCGCAGCTTTCGTCCCAACTTGCAG
                    ************************************************************

HsCD00329703        CTGGCCAACATGGTCCAGATAATTCGCCAGATGTGCCCCACTCCTTATCGGGGAAACCGG
XM_005266000.1      CTGGCCAACATGGTCCAGATAATTCGCCAGATGTGCCCCACTCCTTATCGGGGAAACCGG
NM_032765.2         CTGGCCAACATGGTCCAGATAATTCGCCAGATGTGCCCCACTCCTTATCGGGGAAACCGG
                    ************************************************************

HsCD00329703        AGTAATGATCAGGGCATGTGCTTTAAACACCAGGAAGCCCTGAAACTCTTCTGTGAGGTG
XM_005266000.1      AGTAATGATCAGGGCATGTGCTTTAAACACCAGGAAGCCCTGAAACTCTTCTGTGAGGTG
NM_032765.2         AGTAATGATCAGGGCATGTGCTTTAAACACCAGGAAGCCCTGAAACTCTTCTGTGAGGTG
                    ************************************************************

HsCD00329703        GACAAAGAGGCCATCTGTGTGGTGTGCCGAGAATCCAGGAGCCACAAACAGCACAGCGTG
XM_005266000.1      GACAAAGAGGCCATCTGTGTGGTGTGCCGAGAATCCAGGAGCCACAAACAGCACAGCGTG
NM_032765.2         GACAAAGAGGCCATCTGTGTGGTGTGCCGAGAATCCAGGAGCCACAAACAGCACAGCGTG
                    ************************************************************

HsCD00329703        CTGCCTTTGGAGGAGGTGGTGCAGGAGTACCAGGAAATAAAGTTGGAAACAACTCTGGTG
XM_005266000.1      CTGCCTTTGGAGGAGGTGGTGCAGGAGTACCAGAAGATAGGGTCAACTTCTTCAGTTGAG
NM_032765.2         CTGCCTTTGGAGGAGGTGGTGCAGGAGTACCAGGAAATAAAGTTGGAAACAACTCTGGTG
                    *********************************.*.***..** ...::*::*: * *:*

HsCD00329703        GGAATACTTCAGATAGAGCAAGAAAGCATTCACAGCAAGGCCTATAATCAGTTG
XM_005266000.1      TTATCTGAAAGTGTTGGGCAGTGA------------------------------
NM_032765.2         GGAATACTTCAGATAGAGCAAGAAAGCATTCACAGCAAGGCCTATAATCAGTAA
                      *: : ::.. .*:*.***. .*                              

Protein

SMART domain : BBOX : B-Box-type zinc finger
SMART domain : RING : Ring finger
UniProt : Q96A61
HPRD : 10283

Clone

Cloning Information : 268160
HIP Master Clone ID : 194791
Original Clone ID : FLH268160.01L

Organism


TAX_ID : 9606
Species Specific ID: 84851

Genome

Chromosome : 5
Map Location : 5q35.3

Reagents

Labome : TRIM52-antibody