DNASU Plasmid Repository • 480.965.5697 | Email

ZNF10 (Homo sapiens) in pLenti6.2/V5-DEST (V5-tagged lentiviral mammalian expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  ZNF10
Gene Name:  zinc finger protein 10
Sequence             
Original Clone ID: FLH268163.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLenti6.2/V5-DEST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00329705

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1722nts         Open reading frame : 1 to 1722
Reference Sequence Alignment

ATGGATGCTAAGTCACTAACTGCCTGGTCCCGGACACTGGTGACCTTCAAGGATGTATTT
GTGGACTTCACCAGGGAGGAGTGGAAGCTGCTGGACACTGCTCAGCAGATCGTGTACAGA
AATGTGATGCTGGAGAACTATAAGAACCTGGTTTCCTTGGGTTATCAGCTTACTAAGCCA
GATGTGATCCTCCGGTTGGAGAAGGGAGAAGAGCCCTGGCTGGTGGAGAGAGAAATTCAC
CAAGAGACCCATCCTGATTCAGAGACTGCATTTGAAATCAAATCATCAGTTTCCAGCAGG
AGCATTTTTAAAGATAAGCAATCCTGTGACATTAAAATGGAAGGAATGGCAAGGAATGAT
CTCTGGTATTTGTCATTAGAAGAAGTCTGGAAATGTAGAGACCAGTTAGACAAGTATCAG
GAAAACCCAGAGAGACATTTGAGGCAAGTGGCATTCACCCAAAAGAAAGTACTTACTCAG
GAGAGAGTCTCTGAAAGTGGTAAATATGGGGGAAACTGTCTTCTTCCTGCTCAGCTAGTA
CTGAGAGAGTATTTCCATAAACGTGACTCACATACTAAAAGTTTAAAACATGATTTAGTT
CTTAATGGTCATCAGGACAGTTGTGCAAGTAACAGTAATGAATGTGGTCAAACTTTCTGT
CAAAACATTCACCTTATTCAGTTTGCAAGAACTCACACAGGTGATAAATCCTACAAATGC
CCTGATAATGACAACTCTCTTACTCATGGTTCATCTCTTGGTATATCAAAGGGCATACAT
AGAGAGAAACCCTATGAATGTAAGGAATGTGGAAAATTCTTCAGCTGGCGCTCTAATCTT
ACTAGGCATCAGCTTATTCATACTGGAGAAAAACCGTATGAGTGTAAAGAATGTGGAAAG
TCTTTCAGCCGGAGTTCTCACCTCATTGGACATCAAAAGACCCATACTGGTGAGGAACCC
TATGAATGTAAAGAATGTGGAAAATCCTTCAGCTGGTTCTCTCACCTTGTTACTCATCAG
AGAACTCATACAGGAGACAAACTGTACACATGTAATCAGTGTGGGAAATCTTTTGTTCAT
AGCTCTAGGCTTATTAGACACCAGAGGACACATACTGGAGAGAAACCCTATGAATGTCCT
GAATGTGGGAAATCTTTCAGACAGAGCACACATCTCATTCTGCATCAGAGAACCCATGTG
AGAGTGAGGCCCTATGAATGCAATGAATGTGGAAAGTCTTACAGCCAGAGATCTCACCTT
GTTGTGCATCATAGAATTCACACTGGACTAAAACCTTTTGAGTGTAAGGATTGTGGAAAA
TGTTTTAGTCGAAGCTCTCACCTTTATTCACATCAAAGAACCCACACTGGAGAGAAACCA
TATGAGTGTCATGATTGTGGAAAATCTTTCAGCCAGAGTTCTGCCCTTATTGTGCATCAG
AGGATACACACTGGAGAGAAACCATATGAATGCTGTCAGTGTGGGAAAGCCTTCATCCGG
AAGAATGACCTCATTAAGCACCAGAGAATTCATGTTGGAGAAGAGACCTATAAATGTAAT
CAATGTGGCATTATCTTCAGCCAGAACTCTCCATTTATAGTTCATCAAATAGCTCACACT
GGAGAGCAGTTCTTAACATGCAATCAATGTGGGACAGCGCTTGTTAATACCTCTAACCTT
ATTGGATACCAGACAAATCATATTAGAGAAAATGCTTACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1914
Start on reference sequence 193
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLenti6.2/V5-DEST

pLenti6.2V5-DEST in advanced viewed

Synonyms: pLenti6.2/V5-DEST
Sequencing Primer: Forward:  CMV forward
Reverse:  V5 reverse
Description: Lentiviral mammalian expression vector;CMV promoter and C-terminal V5 tag; ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; Gateway recombinational cloning.
Comments: None
Size (bp): 8813
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pUC ori pUC origin of replication 7733 8406
death cassette ccdB ccdB death cassette (removed when insert is present) 3767 3976
packaging signal HIV-1 psi HIV-1 psi packaging signal 521 565
poly-A signal SV40 SV40 polyadenylation signal 5639 5770
promoter RSV RSV/5' LTR hybrid promoter 1 410
promoter CMV CMV promoter driving gene of interest 1809 2392
promoter PGK PGK promoter to drive selection marker in mammalian cells 4266 4771
recombination site attR2 attR2 recombinational cloning site 4127 4142
recombination site attR1 attR1 recombinational cloning site 2440 2456
response element HIV-1 RRE HIV-1 Rev response element (RRE) 1075 1308
selectable marker AmpR Ampicillin resistance gene for selection in bacteria 6728 7588
selectable marker BlastR Blasticidin resistance gene in mammalian cells 4849 5249
selectable marker CmR chloramphenicol resistance marker (removed in plasmids with insert) 2673 3329
splice acceptor site 3' splice acceptor two 3' splice acceptors at 1656 and 1684 1656 1656
splice donor site 5' splice donor 5' splice donor 520 520
tag V5 C-terminal V5 epitope tag 4197 4238

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF10
Symbol Nomenclature : ZNF10
SYNONYM : KOX1
Designation : zinc finger protein 10 (KOX 1)|zinc finger protein KOX1
Full Nomenclature : zinc finger protein 10
GENEID : 7556
HGNC : 12879
MIM : 194538
Vega : OTTHUMG00000167944
Target GenBank: BC024182

 

Reference Sequence Alignment

  

HsCD00329705      ATGGATGCTAAGTCACTAACTGCCTGGTCCCGGACACTGGTGACCTTCAAGGATGTATTT
NM_015394.4       ATGGATGCTAAGTCACTAACTGCCTGGTCCCGGACACTGGTGACCTTCAAGGATGTATTT
                  ************************************************************

HsCD00329705      GTGGACTTCACCAGGGAGGAGTGGAAGCTGCTGGACACTGCTCAGCAGATCGTGTACAGA
NM_015394.4       GTGGACTTCACCAGGGAGGAGTGGAAGCTGCTGGACACTGCTCAGCAGATCGTGTACAGA
                  ************************************************************

HsCD00329705      AATGTGATGCTGGAGAACTATAAGAACCTGGTTTCCTTGGGTTATCAGCTTACTAAGCCA
NM_015394.4       AATGTGATGCTGGAGAACTATAAGAACCTGGTTTCCTTGGGTTATCAGCTTACTAAGCCA
                  ************************************************************

HsCD00329705      GATGTGATCCTCCGGTTGGAGAAGGGAGAAGAGCCCTGGCTGGTGGAGAGAGAAATTCAC
NM_015394.4       GATGTGATCCTCCGGTTGGAGAAGGGAGAAGAGCCCTGGCTGGTGGAGAGAGAAATTCAC
                  ************************************************************

HsCD00329705      CAAGAGACCCATCCTGATTCAGAGACTGCATTTGAAATCAAATCATCAGTTTCCAGCAGG
NM_015394.4       CAAGAGACCCATCCTGATTCAGAGACTGCATTTGAAATCAAATCATCAGTTTCCAGCAGG
                  ************************************************************

HsCD00329705      AGCATTTTTAAAGATAAGCAATCCTGTGACATTAAAATGGAAGGAATGGCAAGGAATGAT
NM_015394.4       AGCATTTTTAAAGATAAGCAATCCTGTGACATTAAAATGGAAGGAATGGCAAGGAATGAT
                  ************************************************************

HsCD00329705      CTCTGGTATTTGTCATTAGAAGAAGTCTGGAAATGTAGAGACCAGTTAGACAAGTATCAG
NM_015394.4       CTCTGGTATTTGTCATTAGAAGAAGTCTGGAAATGTAGAGACCAGTTAGACAAGTATCAG
                  ************************************************************

HsCD00329705      GAAAACCCAGAGAGACATTTGAGGCAAGTGGCATTCACCCAAAAGAAAGTACTTACTCAG
NM_015394.4       GAAAACCCAGAGAGACATTTGAGGCAAGTGGCATTCACCCAAAAGAAAGTACTTACTCAG
                  ************************************************************

HsCD00329705      GAGAGAGTCTCTGAAAGTGGTAAATATGGGGGAAACTGTCTTCTTCCTGCTCAGCTAGTA
NM_015394.4       GAGAGAGTCTCTGAAAGTGGTAAATATGGGGGAAACTGTCTTCTTCCTGCTCAGCTAGTA
                  ************************************************************

HsCD00329705      CTGAGAGAGTATTTCCATAAACGTGACTCACATACTAAAAGTTTAAAACATGATTTAGTT
NM_015394.4       CTGAGAGAGTATTTCCATAAACGTGACTCACATACTAAAAGTTTAAAACATGATTTAGTT
                  ************************************************************

HsCD00329705      CTTAATGGTCATCAGGACAGTTGTGCAAGTAACAGTAATGAATGTGGTCAAACTTTCTGT
NM_015394.4       CTTAATGGTCATCAGGACAGTTGTGCAAGTAACAGTAATGAATGTGGTCAAACTTTCTGT
                  ************************************************************

HsCD00329705      CAAAACATTCACCTTATTCAGTTTGCAAGAACTCACACAGGTGATAAATCCTACAAATGC
NM_015394.4       CAAAACATTCACCTTATTCAGTTTGCAAGAACTCACACAGGTGATAAATCCTACAAATGC
                  ************************************************************

HsCD00329705      CCTGATAATGACAACTCTCTTACTCATGGTTCATCTCTTGGTATATCAAAGGGCATACAT
NM_015394.4       CCTGATAATGACAACTCTCTTACTCATGGTTCATCTCTTGGTATATCAAAGGGCATACAT
                  ************************************************************

HsCD00329705      AGAGAGAAACCCTATGAATGTAAGGAATGTGGAAAATTCTTCAGCTGGCGCTCTAATCTT
NM_015394.4       AGAGAGAAACCCTATGAATGTAAGGAATGTGGAAAATTCTTCAGCTGGCGCTCTAATCTT
                  ************************************************************

HsCD00329705      ACTAGGCATCAGCTTATTCATACTGGAGAAAAACCGTATGAGTGTAAAGAATGTGGAAAG
NM_015394.4       ACTAGGCATCAGCTTATTCATACTGGAGAAAAACCCTATGAGTGTAAAGAATGTGGAAAG
                  *********************************** ************************

HsCD00329705      TCTTTCAGCCGGAGTTCTCACCTCATTGGACATCAAAAGACCCATACTGGTGAGGAACCC
NM_015394.4       TCTTTCAGCCGGAGTTCTCACCTCATTGGACATCAAAAGACCCATACTGGTGAGGAACCC
                  ************************************************************

HsCD00329705      TATGAATGTAAAGAATGTGGAAAATCCTTCAGCTGGTTCTCTCACCTTGTTACTCATCAG
NM_015394.4       TATGAATGTAAAGAATGTGGAAAATCCTTCAGCTGGTTCTCTCACCTTGTTACTCATCAG
                  ************************************************************

HsCD00329705      AGAACTCATACAGGAGACAAACTGTACACATGTAATCAGTGTGGGAAATCTTTTGTTCAT
NM_015394.4       AGAACTCATACAGGAGACAAACTGTACACATGTAATCAGTGTGGGAAATCTTTTGTTCAT
                  ************************************************************

HsCD00329705      AGCTCTAGGCTTATTAGACACCAGAGGACACATACTGGAGAGAAACCCTATGAATGTCCT
NM_015394.4       AGCTCTAGGCTTATTAGACACCAGAGGACACATACTGGAGAGAAACCCTATGAATGTCCT
                  ************************************************************

HsCD00329705      GAATGTGGGAAATCTTTCAGACAGAGCACACATCTCATTCTGCATCAGAGAACCCATGTG
NM_015394.4       GAATGTGGGAAATCTTTCAGACAGAGCACACATCTCATTCTGCATCAGAGAACCCATGTG
                  ************************************************************

HsCD00329705      AGAGTGAGGCCCTATGAATGCAATGAATGTGGAAAGTCTTACAGCCAGAGATCTCACCTT
NM_015394.4       AGAGTGAGGCCCTATGAATGCAATGAATGTGGAAAGTCTTACAGCCAGAGATCTCACCTT
                  ************************************************************

HsCD00329705      GTTGTGCATCATAGAATTCACACTGGACTAAAACCTTTTGAGTGTAAGGATTGTGGAAAA
NM_015394.4       GTTGTGCATCATAGAATTCACACTGGACTAAAACCTTTTGAGTGTAAGGATTGTGGAAAA
                  ************************************************************

HsCD00329705      TGTTTTAGTCGAAGCTCTCACCTTTATTCACATCAAAGAACCCACACTGGAGAGAAACCA
NM_015394.4       TGTTTTAGTCGAAGCTCTCACCTTTATTCACATCAAAGAACCCACACTGGAGAGAAACCA
                  ************************************************************

HsCD00329705      TATGAGTGTCATGATTGTGGAAAATCTTTCAGCCAGAGTTCTGCCCTTATTGTGCATCAG
NM_015394.4       TATGAGTGTCATGATTGTGGAAAATCTTTCAGCCAGAGTTCTGCCCTTATTGTGCATCAG
                  ************************************************************

HsCD00329705      AGGATACACACTGGAGAGAAACCATATGAATGCTGTCAGTGTGGGAAAGCCTTCATCCGG
NM_015394.4       AGGATACACACTGGAGAGAAACCATATGAATGCTGTCAGTGTGGGAAAGCCTTCATCCGG
                  ************************************************************

HsCD00329705      AAGAATGACCTCATTAAGCACCAGAGAATTCATGTTGGAGAAGAGACCTATAAATGTAAT
NM_015394.4       AAGAATGACCTCATTAAGCACCAGAGAATTCATGTTGGAGAAGAGACCTATAAATGTAAT
                  ************************************************************

HsCD00329705      CAATGTGGCATTATCTTCAGCCAGAACTCTCCATTTATAGTTCATCAAATAGCTCACACT
NM_015394.4       CAATGTGGCATTATCTTCAGCCAGAACTCTCCATTTATAGTTCATCAAATAGCTCACACT
                  ************************************************************

HsCD00329705      GGAGAGCAGTTCTTAACATGCAATCAATGTGGGACAGCGCTTGTTAATACCTCTAACCTT
NM_015394.4       GGAGAGCAGTTCTTAACATGCAATCAATGTGGGACAGCGCTTGTTAATACCTCTAACCTT
                  ************************************************************

HsCD00329705      ATTGGATACCAGACAAATCATATTAGAGAAAATGCTTACTTG
NM_015394.4       ATTGGATACCAGACAAATCATATTAGAGAAAATGCTTACTAA
                  ****************************************:.

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
SMART domain : KRAB : krueppel associated box
UniProt : P21506
UniProt : Q9UG14
HPRD : 01923

Clone

Cloning Information : 268163
HIP Master Clone ID : 194797
Original Clone ID : FLH268163.01L

Organism


TAX_ID : 9606
Species Specific ID: 7556

Genome

Chromosome : 12
Map Location : 12q24.33
Ensembl : ENSG00000256223

Reagents

Labome : ZNF10-antibody